UniProt UniProt http://www.uniprot.org A functional SNP in CILP, encoding cartilage intermediate layer protein, is associated with susceptibility to lumbar disc disease. A functional SNP in CILP, encoding cartilage intermediate layer protein, is associated with susceptibility to lumbar disc disease. Nat. Genet. (1061-4036) 2005 imex curation Seki S., Kawaguchi Y., Chiba K., Mikami Y., Kizawa H., Oya T., Mio F., Mori M., Miyamoto Y., Masuda I., Tsunoda T., Kamata M., Kubo T., Toyama Y., Kimura T., Nakamura Y., Ikegawa S. sikegawa@ims.u-tokyo.ac.jp 27-Mar-2013: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro solid phase assay solid phase assay predetermined predetermined participant predetermined Seki S., Kawaguchi Y., Chiba K., Mikami Y., Kizawa H., Oya T., Mio F., Mori M., Miyamoto Y., Masuda I., Tsunoda T., Kamata M., Kubo T., Toyama Y., Kimura T., Nakamura Y., Ikegawa S. CILP has been used as competitor; % of bound biotinylated TSP1 relative to control has been used as read-out for CILP-binding. sikegawa@ims.u-tokyo.ac.jp Accepted 2013-MAR-25 by MGT 27-Mar-2013: Contacted by IntAct-Help. Nat. Genet. (1061-4036) 2005 Only protein-protein interactions imex curation A functional SNP in CILP, encoding cartilage intermediate layer protein, is associated with susceptibility to lumbar disc disease. A functional SNP in CILP, encoding cartilage intermediate layer protein, is associated with susceptibility to lumbar disc disease. Nat. Genet. (1061-4036) 2005 imex curation Seki S., Kawaguchi Y., Chiba K., Mikami Y., Kizawa H., Oya T., Mio F., Mori M., Miyamoto Y., Masuda I., Tsunoda T., Kamata M., Kubo T., Toyama Y., Kimura T., Nakamura Y., Ikegawa S. sikegawa@ims.u-tokyo.ac.jp 27-Mar-2013: Contacted by IntAct-Help. Only protein-protein interactions human-nucleus pulposus Homo sapiens nucleus pulposus tissue of intervertebral disc nucleus_pulposus Nucleus pulposus tissue of intervertebral disc fluorescence imaging fluorescence microscopy fluorescence imaging immunostaining immunostaining Seki S., Kawaguchi Y., Chiba K., Mikami Y., Kizawa H., Oya T., Mio F., Mori M., Miyamoto Y., Masuda I., Tsunoda T., Kamata M., Kubo T., Toyama Y., Kimura T., Nakamura Y., Ikegawa S. sikegawa@ims.u-tokyo.ac.jp Accepted 2013-MAR-25 by MGT 27-Mar-2013: Contacted by IntAct-Help. Nat. Genet. (1061-4036) 2005 Only protein-protein interactions imex curation tgfb1_human Transforming growth factor beta-1 proprotein TGFB1 TGFB protein protein human Homo sapiens Human MPPSGLRLLLLLLPLLWLLVLTPGRPAAGLSTCKTIDMELVKRKRIEAIRGQILSKLRLASPPSQGEVPPGPLPEAVLALYNSTRDRVAGESAEPEPEPEADYYAKEVTRVLMVETHNEIYDKFKQSTHSIYMFFNTSELREAVPEPVLLSRAELRLLRLKLKVEQHVELYQKYSNNSWRYLSNRLLAPSDSPEWLSFDVTGVVRQWLSRGGEIEGFRLSAHCSCDSRDNTLQVDINGFTTGRRGDLATIHGMNRPFLLLMATPLERAQHLQSSRHRRALDTNYCFSSTEKNCCVRQLYIDFRKDLGWKWIHEPKGYHANFCLGPCPYIWSLDTQYSKVLALYNQHNPGASAAPCCVPQALEPLPIVYYVGRKPKVEQLSNMIVRSCKCS DIP protein P01137 original sequence version: 149 DIP protein P01137 original sequence version: 185 DIP protein P01137 original sequence version: 160 75391614250288FE tsp1_human Thrombospondin-1 THBS1 TSP TSP1 Glycoprotein G protein protein human Homo sapiens Human MGLAWGLGVLFLMHVCGTNRIPESGGDNSVFDIFELTGAARKGSGRRLVKGPDPSSPAFRIEDANLIPPVPDDKFQDLVDAVRAEKGFLLLASLRQMKKTRGTLLALERKDHSGQVFSVVSNGKAGTLDLSLTVQGKQHVVSVEEALLATGQWKSITLFVQEDRAQLYIDCEKMENAELDVPIQSVFTRDLASIARLRIAKGGVNDNFQGVLQNVRFVFGTTPEDILRNKGCSSSTSVLLTLDNNVVNGSSPAIRTNYIGHKTKDLQAICGISCDELSSMVLELRGLRTIVTTLQDSIRKVTEENKELANELRRPPLCYHNGVQYRNNEEWTVDSCTECHCQNSVTICKKVSCPIMPCSNATVPDGECCPRCWPSDSADDGWSPWSEWTSCSTSCGNGIQQRGRSCDSLNNRCEGSSVQTRTCHIQECDKRFKQDGGWSHWSPWSSCSVTCGDGVITRIRLCNSPSPQMNGKPCEGEARETKACKKDACPINGGWGPWSPWDICSVTCGGGVQKRSRLCNNPTPQFGGKDCVGDVTENQICNKQDCPIDGCLSNPCFAGVKCTSYPDGSWKCGACPPGYSGNGIQCTDVDECKEVPDACFNHNGEHRCENTDPGYNCLPCPPRFTGSQPFGQGVEHATANKQVCKPRNPCTDGTHDCNKNAKCNYLGHYSDPMYRCECKPGYAGNGIICGEDTDLDGWPNENLVCVANATYHCKKDNCPNLPNSGQEDYDKDGIGDACDDDDDNDKIPDDRDNCPFHYNPAQYDYDRDDVGDRCDNCPYNHNPDQADTDNNGEGDACAADIDGDGILNERDNCQYVYNVDQRDTDMDGVGDQCDNCPLEHNPDQLDSDSDRIGDTCDNNQDIDEDGHQNNLDNCPYVPNANQADHDKDGKGDACDHDDDNDGIPDDKDNCRLVPNPDQKDSDGDGRGDACKDDFDHDSVPDIDDICPENVDISETDFRRFQMIPLDPKGTSQNDPNWVVRHQGKELVQTVNCDPGLAVGYDEFNAVDFSGTFFINTERDDDYAGFVFGYQSSSRFYVVMWKQVTQSYWDTNPTRAQGYSGLSVKVVNSTTGPGEHLRNALWHTGNTPGQVRTLWHDPRHIGWKDFTAYRWRLSHRPKTGFIRVVMYEGKKIMADSGPIYDKTYAGGRLGLFVFSQEMVFFSDLKYECRDP DIP protein P07996 original sequence version: 142 74749B2418E0943B cilp1_human Cartilage intermediate layer protein 1 CILP Cartilage intermediate-layer protein UNQ602/PRO1188 protein protein human Homo sapiens Human MVGTKAWVFSFLVLEVTSVLGRQTMLTQSVRRVQPGKKNPSIFAKPADTLESPGEWTTWFNIDYPGGKGDYERLDAIRFYYGDRVCARPLRLEARTTDWTPAGSTGQVVHGSPREGFWCLNREQRPGQNCSNYTVRFLCPPGSLRRDTERIWSPWSPWSKCSAACGQTGVQTRTRICLAEMVSLCSEASEEGQHCMGQDCTACDLTCPMGQVNADCDACMCQDFMLHGAVSLPGGAPASGAAIYLLTKTPKLLTQTDSDGRFRIPGLCPDGKSILKITKVKFAPIVLTMPKTSLKAATIKAEFVRAETPYMVMNPETKARRAGQSVSLCCKATGKPRPDKYFWYHNDTLLDPSLYKHESKLVLRKLQQHQAGEYFCKAQSDAGAVKSKVAQLIVIASDETPCNPVPESYLIRLPHDCFQNATNSFYYDVGRCPVKTCAGQQDNGIRCRDAVQNCCGISKTEEREIQCSGYTLPTKVAKECSCQRCTETRSIVRGRVSAADNGEPMRFGHVYMGNSRVSMTGYKGTFTLHVPQDTERLVLTFVDRLQKFVNTTKVLPFNKKGSAVFHEIKMLRRKKPITLEAMETNIIPLGEVVGEDPMAELEIPSRSFYRQNGEPYIGKVKASVTFLDPRNISTATAAQTDLNFINDEGDTFPLRTYGMFSVDFRDEVTSEPLNAGKVKVHLDSTQVKMPEHISTVKLWSLNPDTGLWEEEGDFKFENQRRNKREDRTFLVGNLEIRERRLFNLDVPESRRCFVKVRAYRSERFLPSEQIQGVVISVINLEPRTGFLSNPRAWGRFDSVITGPNGACVPAFCDDQSPDAYSAYVLASLAGEELQAVESSPKFNPNAIGVPQPYLNKLNYRRTDHEDPRVKKTAFQISMAKPRPNSAEESNGPIYAFENLRACEEAPPSAAHFRFYQIEGDRYDYNTVPFNEDDPMSWTEDYLAWWPKPMEFRACYIKVKIVGPLEVNVRSRNMGGTHRQTVGKLYGIRDVRSTRDRDQPNVSAACLEFKCSGMLYDQDRVDRTLVKVIPQGSCRRASVNPMLHEYLVNHLPLAVNNDTSEYTMLAPLDPLGHNYGIYTVTDQDPRTAKEIALGRCFDGTSDGSSRIMKSNVGVALTFNCVERQVGRQSAFQYLQSTPAQSPAAGTVQGRVPSRRQQRASRGGQRQGGVVASLRFPRVAQQPLIN 190B0316404D3B84 tgfb1-thbs1 1 4 unspecified role unspecified role prey prey region biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 5 competitor competitor competitor prey prey purified purified O75339:p.Ile395Thr mutation increasing mutation increasing interaction mutation increasing certain certain sequence position certain certain certain sequence position certain I T Wed May 02 17:00:08 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ile395thr' Tue Jun 30 19:49:47 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ile395Thr' region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined chlae-cos_7 Cercopithecus aethiops simian cells transformed with SV40 Grivet cos_7 cercopithecus aethiops, by transformation with SV40 3 unspecified role unspecified role bait bait association association 4a and b cilp-tgfb1-1 2 3 unspecified role unspecified role neutral component neutral component 5 unspecified role unspecified role neutral component neutral component colocalization colocalization 5c tgfb1-thbs1-1 1 4 unspecified role unspecified role prey prey region biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 3 unspecified role unspecified role bait bait direct interaction direct interaction 4a and b