HPIDb Host Pathogen Interaction database http://www.agbase.msstate.edu/hpi/main.html Hepatitis C virus non-structural protein NS3 interacts with LMP7, a component of the immunoproteasome, and affects its proteasome activity. Hepatitis C virus non-structural protein NS3 interacts with LMP7, a component of the immunoproteasome, and affects its proteasome activity. Biochem. J. (0264-6021) 2004 imex curation Khu Y.L., Tan Y.J., Lim S.G., Hong W., Goh P.Y. mcbgohpy@imcb.nus.edu.sg 17-Jun-2013: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro pull down pull down autoradiography autoradiography Khu Y.L., Tan Y.J., Lim S.G., Hong W., Goh P.Y. mcbgohpy@imcb.nus.edu.sg Accepted 2013-JUN-04 by ORCHARD 17-Jun-2013: Contacted by IntAct-Help. Biochem. J. (0264-6021) 2004 imex curation Hepatitis C virus non-structural protein NS3 interacts with LMP7, a component of the immunoproteasome, and affects its proteasome activity. Hepatitis C virus non-structural protein NS3 interacts with LMP7, a component of the immunoproteasome, and affects its proteasome activity. Biochem. J. (0264-6021) 2004 imex curation Khu Y.L., Tan Y.J., Lim S.G., Hong W., Goh P.Y. mcbgohpy@imcb.nus.edu.sg 17-Jun-2013: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Khu Y.L., Tan Y.J., Lim S.G., Hong W., Goh P.Y. mcbgohpy@imcb.nus.edu.sg Accepted 2013-JUN-04 by ORCHARD 17-Jun-2013: Contacted by IntAct-Help. Human spleen cDNA Biochem. J. (0264-6021) 2004 imex curation Hepatitis C virus non-structural protein NS3 interacts with LMP7, a component of the immunoproteasome, and affects its proteasome activity. Hepatitis C virus non-structural protein NS3 interacts with LMP7, a component of the immunoproteasome, and affects its proteasome activity. Biochem. J. (0264-6021) 2004 imex curation Khu Y.L., Tan Y.J., Lim S.G., Hong W., Goh P.Y. mcbgohpy@imcb.nus.edu.sg 17-Jun-2013: Contacted by IntAct-Help. Only protein-protein interactions imex curation chlae-cos_7 Cercopithecus aethiops simian cells transformed with SV40 Grivet cos_7 cercopithecus aethiops, by transformation with SV40 anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Khu Y.L., Tan Y.J., Lim S.G., Hong W., Goh P.Y. mcbgohpy@imcb.nus.edu.sg Accepted 2013-JUN-04 by ORCHARD 17-Jun-2013: Contacted by IntAct-Help. Biochem. J. (0264-6021) 2004 imex curation Hepatitis C virus non-structural protein NS3 interacts with LMP7, a component of the immunoproteasome, and affects its proteasome activity. Hepatitis C virus non-structural protein NS3 interacts with LMP7, a component of the immunoproteasome, and affects its proteasome activity. Biochem. J. (0264-6021) 2004 imex curation Khu Y.L., Tan Y.J., Lim S.G., Hong W., Goh P.Y. mcbgohpy@imcb.nus.edu.sg 17-Jun-2013: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Khu Y.L., Tan Y.J., Lim S.G., Hong W., Goh P.Y. mcbgohpy@imcb.nus.edu.sg Accepted 2013-JUN-04 by ORCHARD 17-Jun-2013: Contacted by IntAct-Help. Biochem. J. (0264-6021) 2004 imex curation p27958-pro_0000037573 Serine protease/helicase NS3 protein protein HCV77 Hepatitis C virus genotype 1a (isolate H77) APITAYAQQTRGLLGCIITSLTGRDKNQVEGEVQIVSTATQTFLATCINGVCWTVYHGAGTRTIASPKGPVIQTYTNVDQDLVGWPAPQGSRSLTPCTCGSSDLYLVTRHADVIPVRRRGDSRGSLLSPRPISYLKGSSGGPLLCPTGHAVGLFRAAVCTRGVAKAVDFIPVENLETTMRSPVFTDNSSPPAVPQSFQVAHLHAPTGSGKSTKVPAAYAAKGYKVLVLNPSVAATLGFGAYMSKAHGVDPNIRTGVRTITTGSPITYSTYGKFLADAGCSGGAYDIIICDECHSTDATSISGIGTVLDQAETAGARLVVLATATPPGSVTVSHPNIEEVALSTTGEIPFYGKAIPLEVIKGGRHLIFCHSKKKCDELAAKLVALGINAVAYYRGLDVSVIPTSGDVVVVSTDALMTGFTGDFDSVIDCNTCVTQTVDFSLDPTFTIETTTLPQDAVSRTQRRGRTGRGKPGIYRFVAPGERPSGMFDSSVLCECYDAGCAWYELTPAETTVRLRAYMNTPGLPVCQDHLGFWEGVFTGLTHIDAHFLSQTKQSGENFPYLVAYQATVCARAQAPPPSWDQMRKCLIRLKPTLHGPTPLLYRLGAVQNEVTLTHPITKYIMTCMSADLEVVT 1027 1657 5601AD5882D04C8F psa4_human Proteasome subunit alpha type-4 PSMA4 PSC9 Proteasome component C9 Macropain subunit C9 Multicatalytic endopeptidase complex subunit C9 Proteasome subunit L HC9 protein protein human Homo sapiens Human MSRRYDSRTTIFSPEGRLYQVEYAMEAIGHAGTCLGILANDGVLLAAERRNIHKLLDEVFFSEKIYKLNEDMACSVAGITSDANVLTNELRLIAQRYLLQYQEPIPCEQLVTALCDIKQAYTQFGGKRPFGVSLLYIGWDKHYGFQLYQSDPSGNYGGWKATCIGNNSAAAVSMLKQDYKEGEMTLKSALALAIKVLNKTMDVSKLSAEKVEIATLTRENGKTVIRVLKQKEVEQLIKKHEEEEAKAEREKKEKEQKEKDK DIP protein P25789 original sequence version: 160 DIP protein P25789 original sequence version: 133 DIP protein P25789 original sequence version: 114 DIP protein P25789 original sequence version: 149 7867422B1B31F3B9 psb8_human Proteasome subunit beta type-8 PSMB8 LMP7 RING10 Y2 Proteasome subunit beta-5i Proteasome component C13 Macropain subunit C13 Multicatalytic endopeptidase complex subunit C13 Low molecular mass protein 7 PSMB5i Really interesting new gene 10 protein protein protein human Homo sapiens Human MALLDVCGAPRGQRPESALPVAGSGRRSDPGHYSFSMRSPELALPRGMQPTEFFQSLGGDGERNVQIEMAHGTTTLAFKFQHGVIAAVDSRASAGSYISALRVNKVIEINPYLLGTMSGCAADCQYWERLLAKECRLYYLRNGERISVSAASKLLSNMMCQYRGMGLSMGSMICGWDKKGPGLYYVDEHGTRLSGNMFSTGSGNTYAYGVMDSGYRPNLSPEEAYDLGRRAIAYATHRDSYSGGVVNMYHMKEDGWVKVESTDVSDLLHQYREANQ 4F689501677DBD44 ns3-lmp7-1 2 HCV NS3 5 unspecified role unspecified role bait bait LMP7 7 unspecified role unspecified role prey prey physical association physical association p27958_pro-psmb8-2 1 HCV NS3 5 unspecified role unspecified role bait bait ns3 gst gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) LMP7 7 unspecified role unspecified role prey prey lmp7 35smethionine 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined in vitro In vitro physical association physical association 1 psmb8-p27958_pro-1 3 HCV NS3 5 unspecified role unspecified role prey prey nucl transfection nucleic acid transfection nucl transfection region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain ns3 flagtag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined LMP7 7 unspecified role unspecified role bait bait nucl transfection nucleic acid transfection nucl transfection lmp7 myctag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association 2 NS3 N-terminal protease region (aa1-245) interact with LMP7 (fig 2B). LMP7 N-terminal pro-sequence region (aa1-40) interact with NS3 (fig 3C). psma4-ns3-1 4 HCV NS3 5 unspecified role unspecified role prey prey nucl transfection nucleic acid transfection nucl transfection ns3 flagtag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 6 unspecified role unspecified role bait bait nucl transfection nucleic acid transfection nucl transfection lmp7 myctag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association 5 p27958_pro-psmb8-1 3 HCV NS3 5 unspecified role unspecified role bait bait nucl transfection nucleic acid transfection nucl transfection ns3 flagtag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined LMP7 7 unspecified role unspecified role prey prey nucl transfection nucleic acid transfection nucl transfection lmp7 myctag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain physical association physical association 3 NS3 N-terminal protease region (aa1-245) interact with LMP7 (fig 2B). LMP7 N-terminal pro-sequence region (aa1-40) interact with NS3 (fig 3C).