mbinfo MBInfo http://www.mechanobio.info/ Imaging sites of N-wasp activity in lamellipodia and invadopodia of carcinoma cells. Imaging sites of N-wasp activity in lamellipodia and invadopodia of carcinoma cells. Curr. Biol. (0960-9822) 2004 imex curation Lorenz M., Yamaguchi H., Wang Y., Singer R.H., Condeelis J. The source of N-WASP specified by the author. mlorenz@aecom.yu.edu 27-Mar-2013: Contacted by IntAct-Help. Only protein-protein interactions human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. fret fluorescent resonance energy transfer FRET RET FRET analysis FRET RET FRET analysis fret tag fluorescence tag visualisation by fluorescence tag fluorescence Lorenz M., Yamaguchi H., Wang Y., Singer R.H., Condeelis J. The source of N-WASP specified by the author. mlorenz@aecom.yu.edu Accepted 2013-MAR-06 by ORCHARD 27-Mar-2013: Contacted by IntAct-Help. Curr. Biol. (0960-9822) 2004 Only protein-protein interactions imex curation wasl_rat Neural Wiskott-Aldrich syndrome protein Wasl protein protein rat Rattus norvegicus (Rat) Rat MSSGQQPPRRVTNVGSLLLTPQENESLFSFLGKKCVTMSSAVVQLYAADRNCMWSKKCSGVACLVKDNPQRSYFLRIFDIKDGKLLWEQELYNNFVYNSPRGYFHTFAGDTCQVALNFANEEEAKKFRKAVTDLLGRRQRKSEKRRDAPNGPNLPMATVDIKNPEITTNRFYSSQVNNISHTKEKKKGKAKKKRLTKADIGTPSNFQHIGHVGWDPNTGFDLNNLDPELKNLFDMCGISEAQLKDRETSKVIYDFIEKTGGVEAVKNELRRQAPPPPPPSRGGPPPPPPPPHSSGPPPPPARGRGAPPPPPSRAPTAAPPPPPPSRPGVVVPPPPPNRMYPPPPPALPSSAPSGPPPPPPLSMAGSTAPPPPPPPPPPPGPPPPPGLPSDGDHQVPASSGNKAALLDQIREGAQLKKVEQNSRPVSCSGRDALLDQIRQGIQLKSVSDGQESTPPTPAPTSGIVGALMEVMQKRSKAIHSSDEDEDDDDEEDFQDDDEWED DIP protein O08816 original sequence version: 145 DIP protein O08816 original sequence version: 115 480E21F26F7FC77E wasl-1 1 2 unspecified role unspecified role self self n-terminal region cfp tag cyan fluorescent protein tag CFP cyan fluorescent protein cyan fluorescent protein CFP cfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range c-terminal region yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range self interaction self interaction true Fig 1A, 1B, S1, S2. The FRET efficiency decreased in the presence of constitutively active CDC42 and WISH, suggesting the activation of N-WASP, by interfering with the autoinhibitory association of the N- and C-terminal regions of N-WASP. The FRET efficiency remained unchanged in the presence of dominant negative Cdc42 and Rac1.