NTNU NTNU, Norwegian University of Science and Technology www.ntnu.no/home Identification of Basonuclin2, a DNA-binding zinc-finger protein expressed in germ tissues and skin keratinocytes. Identification of Basonuclin2, a DNA-binding zinc-finger protein expressed in germ tissues and skin keratinocytes. Genomics (0888-7543) 2004 imex curation Romano R.A., Li H., Tummala R., Maul R., Sinha S. ssinha2@buffalo.edu 22-Nov-2013: Contacted by IntAct-Help. in vitro In vitro emsa electrophoretic mobility shift assay Gel retardation assay band shift band shift Gel retardation assay emsa weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Romano R.A., Li H., Tummala R., Maul R., Sinha S. ssinha2@buffalo.edu Accepted 2013-NOV-18 by ORCHARD 22-Nov-2013: Contacted by IntAct-Help. Genomics (0888-7543) 2004 imex curation rrna_human_dna human gene promoter -134 to -91 for rRNA gene dna deoxyribonucleic acid DNA deoxyribonucleic acid dna DNA deoxyribonucleic acid chemical synthesis Chemical synthesis (Chemical synthesis) GGTGGCGTTTTTGGGGACAGGTGTCCGTGTCGCGCGTCGCCTGGGCC bnc2_mouse Zinc finger protein basonuclin-2 Bnc2 protein protein mouse Mus musculus Mouse MARFVPSPPPNCLSYKSEGRLGEQDWQAHFKVPCCGVDPSQLESEEAEVDVRERDTQRDREPKRARDLTLRDSCTDNSMQFGTRTTAAEPGFMGTWQNADTNLLFRMSQQVPLACAGRVLGADFCPNLEEPDQRLEVQAIRCTLVNCTCECFQPGKINLRTCDQCKHGWVAHALDKLSTQHLYHPTQVEIVQSNVVFDISSLMLYGTQAVPVRLKILLDRLFSVLKQEEVLHILHGLGWTLRDYVRGYILQDAAGKVLDRWAIMSREEEIITLQQFLRFGETKSIVELMAIQEKEGQAVAVPSSKTDSDIRTFIESNNRTRSPSLLAHLENSNPSSIHHFENIPNSLAFLLPFQYINPVSAPLLGLPPNGLLLEQPGLRLREPSISTQNEYNESSESEVSPTPYKSDQTPNRNALTSITNVEPKTEPACVSPIQNSAPVSDLSKTEHPKSSFRIHRMRRMGSASRKGRVFCNACGKTFYDKGTLKIHYNAVHLKIKHRCTIEGCNMVFSSLRSRNRHSANPNPRLHMPMLRNNRDKDLIRATSGAATPVIASTKSNLTLTSPGRPPMGFTTPPLDPVLQNPLPSQLVFSGLKTVQPVPPFYRSLLTPGEMVSPPTSLPTSPIIPTSGTIEQHPPPPSEPIVPAVMMGTHEPSADLAPKKKPRKSSMPVKIEKEIIDTADEFDDEDDDPNDGGTVVNDMSHDNHCHSQDEMSPGMSVKDFSKHNRTRCISRTEIRRADSMTSEDQEPERDYENESESSEPKLGEESMEGDEHLHSEVSEKVLMNSERPDENHSEPSHQDVIKVKEEFTDPTYDMFYMSQYGLYNGGGASMAALHESFTSSLNYGSPQKFSPEGDLCSSPDPKICYVCKKSFKSSYSVKLHYRNVHLKEMHVCTVAGCNAAFPSRRSRDRHSANINLHRKLLTKELDDMSLDSSQPSLSKDLRDEFLMKIYGAQHPLGLDGREDASSPAGTEDSHLNGYGRGMAEDYMVLDLSTTSSLQSSSSVHSSRESDAGSDEGILLDDIDGASDSGESTHKAEAPTLPGSLGAEVSGSLMFSSLSGSNGGIMCNICHKMYSNKGTLRVHYKTVHLREMHKCKVPGCNMMFSSVRSRNRHSQNPNLHKNIPFTSID 94B2315C52776CEB bsn2-rrna 1 human rRNA gene promoter 2 unspecified role unspecified role prey prey region 32p radiolabel 32p radiolabel P32 32P 32P P32 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined chemical synthesis Chemical synthesis (Chemical synthesis) bsn2 3 unspecified role unspecified role bait bait tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined direct interaction direct interaction Fig 6B