mbinfo MBInfo http://www.mechanobio.info/ ADIP, a novel Afadin- and alpha-actinin-binding protein localized at cell-cell adherens junctions. ADIP, a novel Afadin- and alpha-actinin-binding protein localized at cell-cell adherens junctions. J. Biol. Chem. (0021-9258) 2003 imex curation Asada M., Irie K., Morimoto K., Yamada A., Ikeda W., Takeuchi M., Takai Y. Interaction detection: Yeast two hybrid screen. The source of Afadin inferred from the amino acid length (1829 aa.) specified by the author and the source of ADIP (mouse and rat) inferred from the libraries (mouse embryo and rat brain) used or specified by the author. The source of alpha-actinin inferred from the library used for screening (rat lung). ytakai@molbio.med.osaka-u.ac.jp 17-Jun-2013: Contacted by IntAct-Help. rat lung and rat brain cDNA Only protein-protein interactions canlf-mdck Canis familiaris (dog) kidney epithelial cells Canis lupus familiaris Dog Canis familiaris mdck Canine Cocker Spaniel kidney epithelial cell line fluorescence imaging fluorescence microscopy fluorescence imaging immunostaining immunostaining Asada M., Irie K., Morimoto K., Yamada A., Ikeda W., Takeuchi M., Takai Y. Interaction detection: Yeast two hybrid screen. The source of Afadin inferred from the amino acid length (1829 aa.) specified by the author and the source of ADIP (mouse and rat) inferred from the libraries (mouse embryo and rat brain) used or specified by the author. The source of alpha-actinin inferred from the library used for screening (rat lung). ytakai@molbio.med.osaka-u.ac.jp Accepted 2013-MAY-16 by ORCHARD 17-Jun-2013: Contacted by IntAct-Help. rat lung and rat brain cDNA J. Biol. Chem. (0021-9258) 2003 Only protein-protein interactions imex curation ADIP, a novel Afadin- and alpha-actinin-binding protein localized at cell-cell adherens junctions. ADIP, a novel Afadin- and alpha-actinin-binding protein localized at cell-cell adherens junctions. J. Biol. Chem. (0021-9258) 2003 imex curation Asada M., Irie K., Morimoto K., Yamada A., Ikeda W., Takeuchi M., Takai Y. Interaction detection: Yeast two hybrid screen. The source of Afadin inferred from the amino acid length (1829 aa.) specified by the author and the source of ADIP (mouse and rat) inferred from the libraries (mouse embryo and rat brain) used or specified by the author. The source of alpha-actinin inferred from the library used for screening (rat lung). ytakai@molbio.med.osaka-u.ac.jp 17-Jun-2013: Contacted by IntAct-Help. rat lung and rat brain cDNA Only protein-protein interactions yeast Saccharomyces cerevisiae Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Asada M., Irie K., Morimoto K., Yamada A., Ikeda W., Takeuchi M., Takai Y. Interaction detection: Yeast two hybrid screen. The source of Afadin inferred from the amino acid length (1829 aa.) specified by the author and the source of ADIP (mouse and rat) inferred from the libraries (mouse embryo and rat brain) used or specified by the author. The source of alpha-actinin inferred from the library used for screening (rat lung). ytakai@molbio.med.osaka-u.ac.jp Accepted 2013-MAY-16 by ORCHARD 17-Jun-2013: Contacted by IntAct-Help. rat lung and rat brain cDNA J. Biol. Chem. (0021-9258) 2003 Only protein-protein interactions imex curation ADIP, a novel Afadin- and alpha-actinin-binding protein localized at cell-cell adherens junctions. ADIP, a novel Afadin- and alpha-actinin-binding protein localized at cell-cell adherens junctions. J. Biol. Chem. (0021-9258) 2003 imex curation Asada M., Irie K., Morimoto K., Yamada A., Ikeda W., Takeuchi M., Takai Y. Interaction detection: Yeast two hybrid screen. The source of Afadin inferred from the amino acid length (1829 aa.) specified by the author and the source of ADIP (mouse and rat) inferred from the libraries (mouse embryo and rat brain) used or specified by the author. The source of alpha-actinin inferred from the library used for screening (rat lung). ytakai@molbio.med.osaka-u.ac.jp 17-Jun-2013: Contacted by IntAct-Help. rat lung and rat brain cDNA Only protein-protein interactions in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Asada M., Irie K., Morimoto K., Yamada A., Ikeda W., Takeuchi M., Takai Y. Interaction detection: Yeast two hybrid screen. The source of Afadin inferred from the amino acid length (1829 aa.) specified by the author and the source of ADIP (mouse and rat) inferred from the libraries (mouse embryo and rat brain) used or specified by the author. The source of alpha-actinin inferred from the library used for screening (rat lung). ytakai@molbio.med.osaka-u.ac.jp Accepted 2013-MAY-16 by ORCHARD 17-Jun-2013: Contacted by IntAct-Help. rat lung and rat brain cDNA J. Biol. Chem. (0021-9258) 2003 Only protein-protein interactions imex curation ADIP, a novel Afadin- and alpha-actinin-binding protein localized at cell-cell adherens junctions. ADIP, a novel Afadin- and alpha-actinin-binding protein localized at cell-cell adherens junctions. J. Biol. Chem. (0021-9258) 2003 imex curation Asada M., Irie K., Morimoto K., Yamada A., Ikeda W., Takeuchi M., Takai Y. Interaction detection: Yeast two hybrid screen. The source of Afadin inferred from the amino acid length (1829 aa.) specified by the author and the source of ADIP (mouse and rat) inferred from the libraries (mouse embryo and rat brain) used or specified by the author. The source of alpha-actinin inferred from the library used for screening (rat lung). ytakai@molbio.med.osaka-u.ac.jp 17-Jun-2013: Contacted by IntAct-Help. rat lung and rat brain cDNA Only protein-protein interactions human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Asada M., Irie K., Morimoto K., Yamada A., Ikeda W., Takeuchi M., Takai Y. Interaction detection: Yeast two hybrid screen. The source of Afadin inferred from the amino acid length (1829 aa.) specified by the author and the source of ADIP (mouse and rat) inferred from the libraries (mouse embryo and rat brain) used or specified by the author. The source of alpha-actinin inferred from the library used for screening (rat lung). ytakai@molbio.med.osaka-u.ac.jp Accepted 2013-MAY-16 by ORCHARD 17-Jun-2013: Contacted by IntAct-Help. rat lung and rat brain cDNA J. Biol. Chem. (0021-9258) 2003 Only protein-protein interactions imex curation ADIP, a novel Afadin- and alpha-actinin-binding protein localized at cell-cell adherens junctions. ADIP, a novel Afadin- and alpha-actinin-binding protein localized at cell-cell adherens junctions. J. Biol. Chem. (0021-9258) 2003 imex curation Asada M., Irie K., Morimoto K., Yamada A., Ikeda W., Takeuchi M., Takai Y. Interaction detection: Yeast two hybrid screen. The source of Afadin inferred from the amino acid length (1829 aa.) specified by the author and the source of ADIP (mouse and rat) inferred from the libraries (mouse embryo and rat brain) used or specified by the author. The source of alpha-actinin inferred from the library used for screening (rat lung). ytakai@molbio.med.osaka-u.ac.jp 17-Jun-2013: Contacted by IntAct-Help. rat lung and rat brain cDNA Only protein-protein interactions yeast Saccharomyces cerevisiae Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Asada M., Irie K., Morimoto K., Yamada A., Ikeda W., Takeuchi M., Takai Y. Interaction detection: Yeast two hybrid screen. The source of Afadin inferred from the amino acid length (1829 aa.) specified by the author and the source of ADIP (mouse and rat) inferred from the libraries (mouse embryo and rat brain) used or specified by the author. The source of alpha-actinin inferred from the library used for screening (rat lung). ytakai@molbio.med.osaka-u.ac.jp Accepted 2013-MAY-16 by ORCHARD 17-Jun-2013: Contacted by IntAct-Help. rat lung and rat brain cDNA J. Biol. Chem. (0021-9258) 2003 Only protein-protein interactions imex curation ADIP, a novel Afadin- and alpha-actinin-binding protein localized at cell-cell adherens junctions. ADIP, a novel Afadin- and alpha-actinin-binding protein localized at cell-cell adherens junctions. J. Biol. Chem. (0021-9258) 2003 imex curation Asada M., Irie K., Morimoto K., Yamada A., Ikeda W., Takeuchi M., Takai Y. Interaction detection: Yeast two hybrid screen. The source of Afadin inferred from the amino acid length (1829 aa.) specified by the author and the source of ADIP (mouse and rat) inferred from the libraries (mouse embryo and rat brain) used or specified by the author. The source of alpha-actinin inferred from the library used for screening (rat lung). ytakai@molbio.med.osaka-u.ac.jp 17-Jun-2013: Contacted by IntAct-Help. rat lung and rat brain cDNA Only protein-protein interactions in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Asada M., Irie K., Morimoto K., Yamada A., Ikeda W., Takeuchi M., Takai Y. Interaction detection: Yeast two hybrid screen. The source of Afadin inferred from the amino acid length (1829 aa.) specified by the author and the source of ADIP (mouse and rat) inferred from the libraries (mouse embryo and rat brain) used or specified by the author. The source of alpha-actinin inferred from the library used for screening (rat lung). ytakai@molbio.med.osaka-u.ac.jp Accepted 2013-MAY-16 by ORCHARD 17-Jun-2013: Contacted by IntAct-Help. rat lung and rat brain cDNA J. Biol. Chem. (0021-9258) 2003 Only protein-protein interactions imex curation ADIP, a novel Afadin- and alpha-actinin-binding protein localized at cell-cell adherens junctions. ADIP, a novel Afadin- and alpha-actinin-binding protein localized at cell-cell adherens junctions. J. Biol. Chem. (0021-9258) 2003 imex curation Asada M., Irie K., Morimoto K., Yamada A., Ikeda W., Takeuchi M., Takai Y. Interaction detection: Yeast two hybrid screen. The source of Afadin inferred from the amino acid length (1829 aa.) specified by the author and the source of ADIP (mouse and rat) inferred from the libraries (mouse embryo and rat brain) used or specified by the author. The source of alpha-actinin inferred from the library used for screening (rat lung). ytakai@molbio.med.osaka-u.ac.jp 17-Jun-2013: Contacted by IntAct-Help. rat lung and rat brain cDNA Only protein-protein interactions yeast Saccharomyces cerevisiae Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Asada M., Irie K., Morimoto K., Yamada A., Ikeda W., Takeuchi M., Takai Y. Interaction detection: Yeast two hybrid screen. The source of Afadin inferred from the amino acid length (1829 aa.) specified by the author and the source of ADIP (mouse and rat) inferred from the libraries (mouse embryo and rat brain) used or specified by the author. The source of alpha-actinin inferred from the library used for screening (rat lung). ytakai@molbio.med.osaka-u.ac.jp Accepted 2013-MAY-16 by ORCHARD 17-Jun-2013: Contacted by IntAct-Help. rat lung and rat brain cDNA J. Biol. Chem. (0021-9258) 2003 Only protein-protein interactions imex curation ADIP, a novel Afadin- and alpha-actinin-binding protein localized at cell-cell adherens junctions. ADIP, a novel Afadin- and alpha-actinin-binding protein localized at cell-cell adherens junctions. J. Biol. Chem. (0021-9258) 2003 imex curation Asada M., Irie K., Morimoto K., Yamada A., Ikeda W., Takeuchi M., Takai Y. Interaction detection: Yeast two hybrid screen. The source of Afadin inferred from the amino acid length (1829 aa.) specified by the author and the source of ADIP (mouse and rat) inferred from the libraries (mouse embryo and rat brain) used or specified by the author. The source of alpha-actinin inferred from the library used for screening (rat lung). ytakai@molbio.med.osaka-u.ac.jp 17-Jun-2013: Contacted by IntAct-Help. rat lung and rat brain cDNA Only protein-protein interactions mouse-intestinal_epitheliu Mouse intestinal epithelial cells intestinal_epithelium Intestinal epithelial cells May include "microvilli". fluorescence imaging fluorescence microscopy fluorescence imaging immunostaining immunostaining Asada M., Irie K., Morimoto K., Yamada A., Ikeda W., Takeuchi M., Takai Y. Interaction detection: Yeast two hybrid screen. The source of Afadin inferred from the amino acid length (1829 aa.) specified by the author and the source of ADIP (mouse and rat) inferred from the libraries (mouse embryo and rat brain) used or specified by the author. The source of alpha-actinin inferred from the library used for screening (rat lung). ytakai@molbio.med.osaka-u.ac.jp Accepted 2013-MAY-16 by ORCHARD 17-Jun-2013: Contacted by IntAct-Help. rat lung and rat brain cDNA J. Biol. Chem. (0021-9258) 2003 Only protein-protein interactions imex curation ADIP, a novel Afadin- and alpha-actinin-binding protein localized at cell-cell adherens junctions. ADIP, a novel Afadin- and alpha-actinin-binding protein localized at cell-cell adherens junctions. J. Biol. Chem. (0021-9258) 2003 imex curation Asada M., Irie K., Morimoto K., Yamada A., Ikeda W., Takeuchi M., Takai Y. Interaction detection: Yeast two hybrid screen. The source of Afadin inferred from the amino acid length (1829 aa.) specified by the author and the source of ADIP (mouse and rat) inferred from the libraries (mouse embryo and rat brain) used or specified by the author. The source of alpha-actinin inferred from the library used for screening (rat lung). ytakai@molbio.med.osaka-u.ac.jp 17-Jun-2013: Contacted by IntAct-Help. rat lung and rat brain cDNA Only protein-protein interactions canlf-mdck Canis familiaris (dog) kidney epithelial cells Canis lupus familiaris Dog Canis familiaris mdck Canine Cocker Spaniel kidney epithelial cell line anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Asada M., Irie K., Morimoto K., Yamada A., Ikeda W., Takeuchi M., Takai Y. Interaction detection: Yeast two hybrid screen. The source of Afadin inferred from the amino acid length (1829 aa.) specified by the author and the source of ADIP (mouse and rat) inferred from the libraries (mouse embryo and rat brain) used or specified by the author. The source of alpha-actinin inferred from the library used for screening (rat lung). ytakai@molbio.med.osaka-u.ac.jp Accepted 2013-MAY-16 by ORCHARD 17-Jun-2013: Contacted by IntAct-Help. rat lung and rat brain cDNA J. Biol. Chem. (0021-9258) 2003 Only protein-protein interactions imex curation ADIP, a novel Afadin- and alpha-actinin-binding protein localized at cell-cell adherens junctions. ADIP, a novel Afadin- and alpha-actinin-binding protein localized at cell-cell adherens junctions. J. Biol. Chem. (0021-9258) 2003 imex curation Asada M., Irie K., Morimoto K., Yamada A., Ikeda W., Takeuchi M., Takai Y. Interaction detection: Yeast two hybrid screen. The source of Afadin inferred from the amino acid length (1829 aa.) specified by the author and the source of ADIP (mouse and rat) inferred from the libraries (mouse embryo and rat brain) used or specified by the author. The source of alpha-actinin inferred from the library used for screening (rat lung). ytakai@molbio.med.osaka-u.ac.jp 17-Jun-2013: Contacted by IntAct-Help. rat lung and rat brain cDNA Only protein-protein interactions human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Asada M., Irie K., Morimoto K., Yamada A., Ikeda W., Takeuchi M., Takai Y. Interaction detection: Yeast two hybrid screen. The source of Afadin inferred from the amino acid length (1829 aa.) specified by the author and the source of ADIP (mouse and rat) inferred from the libraries (mouse embryo and rat brain) used or specified by the author. The source of alpha-actinin inferred from the library used for screening (rat lung). ytakai@molbio.med.osaka-u.ac.jp Accepted 2013-MAY-16 by ORCHARD 17-Jun-2013: Contacted by IntAct-Help. rat lung and rat brain cDNA J. Biol. Chem. (0021-9258) 2003 Only protein-protein interactions imex curation adip_mouse Afadin- and alpha-actinin-binding protein Ssx2ip Afadin DIL domain-interacting protein protein protein mouse Mus musculus Mouse MGDWMTVTDPVLCTENKNLSQYTSETKMSPSSLYSQQVLCSSVPLSKNVHGVFGVFCTGENIEQSISYLDQELTTFGFPSLYEESKSKEAKRELNIVAVLNCMNELLVLQRKNLLAQESVETQNLKLGSDMDHLQSCYAKLKEQLETSRREMIGLQERDRQLQCKNRSLHQLLKNEKDEVQKLQNIIASRATQYNHDVKRKEREYNKLKERLHQLVMNKKDKNIAMDVLNYVGRADGKRGSWRTDKTEARNEDEMYKILLNDYEYRQKQILMENAELKKVLQQMKKEMISLLSPQKKKPRERAEDGTGTVAISDIEDDSGELSRDSVWGLSCDTVREQLTNSIRKQWRILKSHVEKLDNQASKVHSEGLNEEDVISRQDHEQETEKLELEIERCKEMIKAQQQLLQQQLATTCDDDTTSLLRDCYLLEEKERLKEEWTLFKEQKKNFERERRSFTEAAIRLGLERKAFEEERASWVKQQFLNMTNFDHQNSENVKLFSAFSGSSDPDNLIVHSRPRQKKLHSVANGVPACTSKLTKSLPASPSTSDFRQTHSCVSEHSSISVLNITPEESKPSEVARESTDQKWSVQSRPSSREGCYSGCSSAFRSAHGDRDDLP CC41D7078591919F actn1_human Alpha-actinin-1 ACTN1 Alpha-actinin cytoskeletal isoform Non-muscle alpha-actinin-1 F-actin cross-linking protein protein protein human Homo sapiens Human MDHYDSQQTNDYMQPEEDWDRDLLLDPAWEKQQRKTFTAWCNSHLRKAGTQIENIEEDFRDGLKLMLLLEVISGERLAKPERGKMRVHKISNVNKALDFIASKGVKLVSIGAEEIVDGNVKMTLGMIWTIILRFAIQDISVEETSAKEGLLLWCQRKTAPYKNVNIQNFHISWKDGLGFCALIHRHRPELIDYGKLRKDDPLTNLNTAFDVAEKYLDIPKMLDAEDIVGTARPDEKAIMTYVSSFYHAFSGAQKAETAANRICKVLAVNQENEQLMEDYEKLASDLLEWIRRTIPWLENRVPENTMHAMQQKLEDFRDYRRLHKPPKVQEKCQLEINFNTLQTKLRLSNRPAFMPSEGRMVSDINNAWGCLEQVEKGYEEWLLNEIRRLERLDHLAEKFRQKASIHEAWTDGKEAMLRQKDYETATLSEIKALLKKHEAFESDLAAHQDRVEQIAAIAQELNELDYYDSPSVNARCQKICDQWDNLGALTQKRREALERTEKLLETIDQLYLEYAKRAAPFNNWMEGAMEDLQDTFIVHTIEEIQGLTTAHEQFKATLPDADKERLAILGIHNEVSKIVQTYHVNMAGTNPYTTITPQEINGKWDHVRQLVPRRDQALTEEHARQQHNERLRKQFGAQANVIGPWIQTKMEEIGRISIEMHGTLEDQLSHLRQYEKSIVNYKPKIDQLEGDHQLIQEALIFDNKHTNYTMEHIRVGWEQLLTTIARTINEVENQILTRDAKGISQEQMNEFRASFNHFDRDHSGTLGPEEFKACLISLGYDIGNDPQGEAEFARIMSIVDPNRLGVVTFQAFIDFMSRETADTDTADQVMASFKILAGDKNYITMDELRRELPPDQAEYCIARMAPYTGPDSVPGALDYMSFSTALYGESDL 6DA3E4D1A0289519 f1psu6_canlf AFDN protein protein canlf Canis familiaris (dog) Canis lupus familiaris Dog Canis familiaris MRFYFQDKAAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGEERRLDIDEKPLVVQLNWNKDDREGRFVLKNENDALAPKKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKREKEAVRQASDKDDRPFQGDDIENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLERRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDPADFAVAEALEKYGLEKENPKDYCIARVMLPPGAQHSDEKGAKEMILDDDECPLQIFREWPSDKGLLVFQLKRRPADYIPKKTKKHVDGKLVKGKERAEGSAYGSALPPEKLPYLVELSPGRRNHFAYYNYHTYEDGSDSRDKPKLYRLQLSVTEVGTEKFDENSIQLFGSGIQPHHCDLTNMDGVVTVTPRSMDAETYVEGQRISETTMLQSGMKVQFGASHVFKFVDPSQDHALAKRSVDGSLTAKGPRHKPGIVQETTFDLGGDVHSGTALPTSKSTTRLDGDRVLSASSTAERGMVKPMMRVEQQQDYRRQENRTQDAPGPELILPASIEFRESSEDSFLSAIINYTNSSTVHFKLSPTYVLYMACRYVLSSQYRPDVSPTERTHKVIAIVNKMVSMMEGVIQEVDQVDQKQKNIAGALAFWMANASELLNFIKQDRDLSRITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFLDDPEENSLQRPKIDDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNRLVTDPESGLCSHYWGAIIRQQLGHVEAWAEKQGLELAADCHLSRIVQATTLLTMDKYAPDDIPNINSTCFKLNSLQLQALLQNYHCAPDEPFIPTDLIENVVAVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCDVVRNIPNGLQEFLDPLCQRGFCRLIPHARSPGTWTIYFEGADYESHLLRENTELAQPLRKEPEIITVTLKKQNGMGLSIVAAKGAGQDKLGIYVKSVVKGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTSSVVTLEVAKQGAIYHGLATLLNQPSPMMQRISDRRGSGKPRPKSEGFELYNNSAQNGSPESPQLPWAEYTEPKKLPGDDRLMKNRADHRSSPNVANQPPSPGGKSAYAPGTTAKITSVSTGNLCTEEQTPPPRPEAYPIPTQTYTREYFTFPASKSQDRMAPPQNQWPNYEEKPHMHMDNNHSSIAIQRVTRSQEELREDKAYQLERHRIEAVLDRKSDSDMWIHQSSSVGSSTSSQEHLDHPSKAGTPASTLTKSGPGRWKTPAAVQPLPVAGSQPVQTDLPPPPPPPPTHYAGDFDGIPTDLPLPPPPASQTGPASAQVVAAERKKREEHQRWYEKEKARLEEERDRKRREQERKLGQMRSQSLNPAPFSPVTVPHVKPEKPSTLQRPQETVIRELQPQQQPRTIERRDLQYITVSKEELSSGDSLSPDPWKRDAREKLEKQQQMHIVDMLSREIQELQNKPERSAEENDRLRKLMLEWQFQKRLQESKQKDEDDEEEEDDDVDTMLIMQRLEAERRARQTALPAISVLDLLQDEERRRQQQLEEMRKREAEDRARQEDERRRQEEERAKRDAEEKRRQEEGYYSRLEAERRRQHAEAERRLLEPEEPGLCRPPLPRGYKLPSPSPPAAAPPPPPQRTASSLQAQALSPDSLYTARLVAYHEEEEEEHCSLAGPNSYLGSAGTAVGTHDAYRDPRERLSKSHEADLPGSSGAPENLTFKERQRLFSQGQDVSNKVKASRKLTELENELNTK 2F326C028B805AA0 actn1_rat Alpha-actinin-1 Actn1 Alpha-actinin cytoskeletal isoform Non-muscle alpha-actinin-1 F-actin cross-linking protein protein protein rat Rattus norvegicus (Rat) Rat MDHYDSQQTNDYMQPEEDWDRDLLLDPAWEKQQRKTFTAWCNSHLRKAGTQIENIEEDFRDGLKLMLLLEVISGERLAKPERGKMRVHKISNVNKALDFIASKGVKLVSIGAEEIVDGNVKMTLGMIWTIILRFAIQDISVEETSAKEGLLLWCQRKTAPYKNVNIQNFHISWKDGLGFCALIHRHRPELIDYGKLRKDDPLTNLNTAFDVAERYLDIPKMLDAEDIVGTARPDEKAIMTYVSSFYHAFSGAQKAETAANRICKVLAVNQENEQLMEDYEKLASDLLEWIRRTIPWLENRVPENTMQAMQQKLEDFRDYRRLHKPPKVQEKCQLEINFNTLQTKLRLSNRPAFMPSEGRMVSDINNAWGCLEQAEKGYEEWLLNEIRRLERLDHLAEKFRQKASIHEAWTDGKEAMLRQKDYETATLSEIKALLKKHEAFESDLAAHQDRVEQIAAIAQELNELDYYDSPSVNARCQKICDQWDNLGALTQKRREALERTEKLLETIDQLYLEYAKRAAPFNNWMEGAMEDLQDTFIVHTIEEIQGLTTAHEQFKATLPDADKERLAILGIHNEVSKIVQTYHVNMAGTNPYTTITPQEINGKWDHVRQLVPRRDQALTEEHSRQQHNERLRKQFGAQANVIGPWIQTKMEEIGRISIEMHGTLEDQLSHLRQYEKSIVNYKPKIDQLEGDHQLIQEALIFDNKHTNYTMEHIRVGWEQLLTTIARTINEVENQILTRDAKGISQEQMNEFRASFNHFDRDHSGTLGPEEFKACLISLGYDIGNDPQGEAEFARIMSIVDPNRLGVVTFQAFIDFMSRETADTDTADQVMASFKILAGDKNYITGDELRRELPPDQAEYCIARMAPYAGPDSVPGALDYMSFSTALYGESDL 2360D496D0A84095 e2qy08_canlf ACTN1 protein protein canlf Canis familiaris (dog) Canis lupus familiaris Dog Canis familiaris MDHYDSQQTNDYMQPEEDWDRDLLLDPAWEKQQRKTFTAWCNSHLRKAGTQIENIEEDFRDGLKLMLLLEVISGERLAKPERGKMRVHKISNVNKALDFIASKGVKLVSIGAEEIVDGNVKMTLGMIWTIILRFAIQDISVEETSAKEGLLLWCQRKTAPYKNVNIQNFHISWKDGLGFCALIHRHRPELIDYGKLRKDDPLTNLNTAFDVAEKYLDIPKMLDAEDIVGTARPDEKAIMTYVSSFYHAFSGAQKAETAANRICKVLAVNQENEQLMEDYEKLASDLLEWIRRTIPWLENRAPENTMHAMQQKLEDFRDYRRLHKPPKVQEKCQLEINFNTLQTKLRLSNRPAFMPSEGRMVSDINNAWGCLEQAEKGYEEWLLNEIRRLERLDHLAEKFRQKASIHEAWTDGKEAMLRQKDYETATLSEIKALLKKHEAFESDLAAHQDRVEQIAAIAQELNELDYYDSPSVNARCQKICDQWDNLGALTQKRREALERTEKLLETIDQLYLEYAKRAAPFNNWMEGAMEDLQDTFIVHTIEEIQGLTTAHEQFKATLPDADKERLAILGIHNEVSKIVQTYHVNMAGTNPYTTITPQEINGKWDHVRQLVPRRDQALTEEHARQQHNERLRKQFAAQANVIGPWIQTKMEEIGRISIEMHGTLEDQLSHLRQYEKSIVNYKPKIDQLEGDHQLIQEALIFDNKHTNYTMEHIRVGWEQLLTTIARTINEVENQILTRDAKGISQEQMNEFRASFNHFDRDHSGTLGPEEFKACLISLGYDIGNDPQKKTGMMDTDDFRACLISMGYNMGEAEFARIMSIVDPNRLGVVTFQAFIDFMSRETADTDTADQVMASFKILAGDKNYITVDELRRELPPDQAEYCIARMAPYSGPDAVPGALDYMSFSTALYGESDL 4D0AF2B81FE77713 myo4_yeast Myosin-4 MYO4 SHE1 YAL029C FUN22 SWI5-dependent HO expression protein 1 protein protein yeast Saccharomyces cerevisiae Baker's yeast MSFEVGTKCWYPHKEQGWIGGEVTKNDFFEGTFHLELKLEDGETVSIETNSFENDDDHPTLPVLRNPPILESTDDLTTLSYLNEPAVLHAIKKRYMNGQIYTYSGIVLIAANPFDKVDHLYSREMIQNYSSKRKDELEPHLFAIAEEAYRFMVHEKANQTVVVSGESGAGKTVSAKYIMRYFASVQESNNREGEVEMSQIESQILATNPIMEAFGNAKTTRNDNSSRFGKYLQILFDENTTIRGSKIRTYLLEKSRLVYQPETERNYHIFYQILEGLPEPVKQELHLSSPKDYHYTNQGGQPNIAGIDEAREYKITTDALSLVGINHETQLGIFKILAGLLHIGNIEMKMTRNDASLSSEEQNLQIACELLGIDPFNFAKWIVKKQIVTRSEKIVTNLNYNQALIARDSVAKFIYSTLFDWLVDNINKTLYDPELDQQDHVFSFIGILDIYGFEHFEKNSFEQFCINYANEKLQQEFNQHVFKLEQEEYVKEEIEWSFIEFSDNQPCIDLIENKLGILSLLDEESRLPSGSDESWASKLYSAFNKPPSNEVFSKPRFGQTKFIVSHYAVDVEYEVEGFIEKNRDSVSLGHLDVFKATTNPIFKQILDNRELRSDDAPEEQNTEKKIMIPARLSQKKPTLGSMFKKSLGELMAIINSTNVHYIRCIKPNSEKKPWEFDNLMVLSQLRACGVLETIRISCAGFPSRWTFDEFVQRYFLLTDYSLWSGILYNPDLPKEAIVNFCQSILDATISDSAKYQIGNTKIFFKAGMLAFLEKLRTNKMNEICIIIQKKIRARYYRLQYLQTMESIKKCQSQIRSLLVRTRVDHELKTRAAILLQTNIRALWKREYYRAAIGQIIKLQCTCKRKLILDSVNRKFMLMAAVIIQSYIRSYGHKTDYRTLKRSSILVQSAMRMQLARRRYIVLQKEVEERNIRASYGIGLLEEAIEFKNSFILNLEMLNDSYTRLTQLLQGDLSNIPSKQRQEYETIVNGYNDKISKLKTLQVEIMNTLNKKNALKERKKKQSSLIQSHMQSLAAIKGNKPSRLSDEVKSMKQELAFIENVIAQDFTTTYSANKNDKVKGLGIAGQQVKPKLVNVIRRESGNPDLLELLMDLNCYTLEVTEGYLKKVNVTEVNGDNVLGPIHVITTVVSSLVRNGLLIQSSKFISKVLLTVESIVMSLPKDETMLGGIFWLSNLSRLPAFAANQKTLYEANGGDEKDKLTLIYLNDLENETLKVFDKIYSTWLVKFMKHASAHIEIFDMVLNEKLFKNSGDEKFAKLFTFLNEFDAVLCKFQVVDSMHTKIFNDTLKYLNVMLFNDLITKCPALNWKYGYEVDRNIERLVSWFEPRIEDVRPNLIQIIQAVKILQLKISNLNEFKLLFDFWYALNPAQIQAILLKYKPANKGEAGVPNEILNYLANVIKRENLSLPGKMEIMLSAQFDSAKNHLRYDTSAITQNSNTEGLATVSKIIKLDRK E79C0FE72B041E95 E79C0FE72B041E95 afad_mouse Afadin Afdn Af6 Afadin adherens junction formation factor Mllt4 Protein Af-6 protein protein mouse Mus musculus Mouse MSAGGRDEERRKLADIIHHWNANRLDLFEISQPTEDLEFHGVMRFYFQDKAAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGERRLDIDEKPLVVQLNWNKDDREGRFVLKNENDAIPAKKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKKEKEALRQASDKEERPSQGDDSENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLEKRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDTADFAVAESLEKYGLEKENPKDYCIARVMLPPGAQHSDERGAKEIILDDDECPLQIFREWPSDKGILVFQLKRRPPDYIPKKMKKHVEGKSLKGKDRADGSGYGSALPPEKLPYLVELSPGRRNHFAYYSYHTYEDGSDSRDKPKLYRLQLSVTEVGTEKFDDNSIQLFGPGIQPHHCDLTNMDGVVTVTPRSMDAETYVDGQRISETTMLQSGMRLQFGTSHVFKFVDPIQDHVLSKRSVDGGLMVKGPRHKPGAVQETTFELGGDVHSGTALPASRSTTRLDSDRVSSASSTAERGMVKPMIRLDQEQEYRRRENRTQDATGPELILPASIEFRESSEDSFLSAIINYTNSSTVHFKLSPTYVLYMACRYVLSSQHRPDISPTERTHKAIAVVNKMVSMMEGVIQKQKNIAGALAFWMANASELLNFIKQDRDLSRITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFLDDPEENSLQRPKIDDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNRLVTDPDSGLCSHYWGAIIRQQLGHIEAWAEKQGLELAADCHLSRIVQATTLLTMDKYVPDDIPNINSTCFKLNSLQLQALLQNYHCAPDEPFIPTDLIENVVAVAENTADELARSDGRDVQLEEDPDLQLPFLLPEDGYSCDVVRNIPNGLQEFLDPLCQRGFCRLVPHTRSPGTWTIYFEGADYESHLMRENAELAQPLRKEPEIITVTLKKQNGMGLSIVAAKGAGQDKLGIYVKSVVKGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTSSVVTLEVAKQGAIYHGLATLLNQPSPMMQRISDRRGSGKPRPKSEGFELYNNSAQNGSPESPQMPWTEYSEPKKLPGDDRLMKNRADHRSSPNVANQPPSPGGKGPYTSGTAAKITSVSTGNLCTEEQSPPPRPEAYPIPTQTYTREYFTFPASKSQDRMAPPQSQWPNYEEKPHVHTESNHSSIAIQRVTRSQEELREEKVYQLERHRVEAGMDRKCDSDMWINQSSSVESSTSSQEHLNHSSKSVTPASTLTKSGPGRWKTPAAVLPTPVAVSQPIRTDLPPPPPPPPVHYTSEFDGIPMDLPLPPPPANQAGPQSAQVAAAEWKKREEHQRWYEKEKARLEEERERKRREQERKLGQMRSQTLNPASFSPLATQAKPEKPSTLQRPQETVIRELQPQQQPRTIERKDLQYITISKEELSSGDSLSPDPWKRDAREKLEKQQQMHIVDMLSKEIHELQNKVDRTAEESDRLRKLMLEWQFQKRLQESKQKDEDDDEEEDDDVDTMLIMQRLEAERRARMQDEERRRQQQLEEMRKREAEDRVRQEEDGRHQEEERVKRDAEEKRRQEEGYYSRLEAERRRQHEEAARRLLEPEEPGLSRPPLPRDYEPPSLSSAPCAPPPPPQRNASYLKTQVLSPDSLFTAKFVAYDEEEEDYGPAGPNSYSGSAGTAVGAYDAPREAREKLTRSQDADLPGSSGAPENLTFKERQRLFSQGQDVSDKVKASRKLTELENELNTK DIP protein Q9QZQ1 original sequence version: 107 9FA4F378D840D8B1 adip_rat Afadin- and alpha-actinin-binding protein Ssx2ip Afadin DIL domain-interacting protein protein protein rat Rattus norvegicus (Rat) Rat MGDWMTVTDPVLCTENKNLSQYTSETKMSPSSLYSQQVLCSATPLSKNVHGVFSAFCTGENIEQSISYLDQELTTFGFPSLYEESKSKEAKRELSIVALLNCMNELLVLQRKNLLAQESVETQNLKLGSDMDHLQSCYAKLKEQLEASRREMISLQERDRQLQCKNRNLHQLLKNEKEEVQKLQNIIASRATQYNHDVKRKEREYNKLKERLHQLVMNKKDKNIAMDVLNYVGRVDGKRGSWRTDKTEARNEDEMYKILLNDYEYRQKQILLENAELKKVLQQMKKEMISLLSPQKKKPRERAEDSTGTVVISDVEDDAGELSRDGVWSLSCDTVREQLTNSIRKQWRILKSHVEKLDNQASKVHSEGFHEEDVISRQDHEQETEKLELEIERCKEMIKAQQQLLQQQLATACDDDTTSLLRDCYLLEEKERLKEEWSLFKEQKKNFERERRSFTEAAIRLGLERKAFEEERASWVKQQFLNMTTFDHQNSENVKLFSAFSGSSDPDNLIVHPRPRQKKPHSVANGVPACTSKLAKSLPTSPSDFCPSRSCVSEHSPVSALTVTPEETKPNEVGRESTDQKWSVVSRPSSREGCYGGCSSAYTSSHVERDDLP 55A6174A810AB8DE afad_rat Afadin Afdn Af6 Afadin adherens junction formation factor Mllt4 Protein Af-6 protein protein rat Rattus norvegicus (Rat) Rat MSAGGRDEERRKLADIIHHWNANRLDLFEISQPTEDLEFHGVMRFYFQDKAAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGERRLDIDEKPLVVQLNWNKDDREGRFVLKNENDAIPAKKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKREKEALRQASDKEERPSQGDDSENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLEKRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDPADFAVAESLEKYGLEKENPKDYCIARVMLPPGAQHSDERGAKEIILDDDECPLQIFREWPSDKGILVFQLKRRPPDYIPKKMKKHVEGKPLKGKDRADGSGYGSALPPEKLPYLVELSPGRRNHFAYYSYHTYEDGSDSRDKPKLYRLQLSVTEVGTEKFDDNSIQLFGPGIQPHHCDLTNMDGVVTVTPRSMDAETYVDGQRISETTMLQSGMRLQFGTSHVFKFVDPIQDHVLSKRSVDGGLMVKGPRHKPGAVQETTFELGGDIHSGTALPASRSTTRLDSDRVSSASSTAERGMVKPMIRLDQEQDYRRRESRTQDAAGPELMLPASIEFRESSEDSFLSAIINYTNSSTVHFKLSPTYVLYMACRYVLSSQHRPDISPTERTHKAIAVVNKMVSMMEGVIQEVDQVDQKQKNIAGALAFWMANASELLNFIKQDRDLSRITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFLDDPEENSLQRPKIDDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNRLVTDPDSGLCSHYWGAIIRQQLGHIEAWAEKQGLELAADCHLSRIVQATTLLTMDKYVPDDIPNINSTCFKLNSLQLQALLQNYHCAPDEPFIPTDLIENVVAVAENTADELARSDGRDVQLEEDPDLQLPFLLPEDGYSCDVVRNIPNGLQEFLDPLCQRGFCRLVPHTRSPGTWTIYFEGADYESHLMRENTELTQPLRKEPEVITVTLKKQNGMGLSIVAAKGAGQDKLGIYVKSVVKGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTSSVVTLEVAKQGAIYHGLATLLNQPSPMMQRISDRRGSGKPRPKSEGFELYNNSAQNGSPESPQMPWTEYSEPKKLPGDDRLMKNRADHRSSPNVANQPPSPGGKSPYTSGTAAKITSVSTGNLCTEEQTPPPRPEAYPIPTQTYTREYFTFPASKSQDRMAPVQNQWPNYEEKPHMHTESDHASIAIQRVTRSQEELREEKVYQLERHRVESGMDRKCDSDMWINQSSSVESSTSSQEHLNHSSKSVTPASTLTKSGPGRWKTPAAVLPTPVAVSQPIRTDLPPPPPPPPAHYTSDFDGISMDLPLPPPPANQAAPQSAQVAAAERKKREEHQRWYEKEKARLEEERERKRREQERKLGQMRTQSLNPASFSPLATQAKPEKPSTLQRPQETVIRELQPQQQPRTIERRDLQYITISKEELSSGDSLSPDPWKRDAREKLEKQQQMHIVDMLSKEIHELQNKGDRTAEESDRLRKLMLEWQFQKRLQESKQKDEDDDEEEDDDVDTMLIMQRLEAERRARLQDEERRRQQQLEEMRKREVEDRVRQEEDGRHQEEERVKRDAEEKRRQEEGYYSRLEAERRRQHEEAARRLLEPEEPGLSRPPLPQDYEPPSQSSAPSAPPPPPQRNASYLKTQVLSPDSLFTAKFVAYDDDDEEENYVPAGPNSYSGSAGTTAGTYDAPRDTREKLSRSQDADLPGSSGAPENLTFRERQRLFSQGQDVSDKVKASRKLTELENELNTK 45C597A82F109D6F f1paa2_canlf SSX2IP protein protein canlf Canis familiaris (dog) Canis lupus familiaris Dog Canis familiaris MGEWMTVTDPDLSSESKNICQYTSETKMSPSSLYSQQVLCSSIPLSKNVHSFFSAFCTEENIEQSISYLDQELTTFGFPSLYEESKGKDTKRELNIVAVLNCMNELLVLQRKNLLAQENVETQNLKLGSDMDHLQNCYAKLKEQLETSRREMIGLQERDRQLQCKNRNLHQLLKNEKDEVQKLQNIIASRATQYNHDMKRKEREYNKLKERLHQLVMNKKDKKIAMEVLNYVGRADGKRGSWRTGKTEARNEDEMYKILLNDYEYRQKQILMENAELKKVLQQMKKEMISLLSPQKQKPRERADDSTGTVISDAEEDAGELSRESMWDLSCETVREQLTNSIRKQWRILKSHVEKLDNQVSKVHLEGFTDEDVISRQDHEQETEKLELEIQQCKEMIKTQQQLLQQLATACDDDTTSLLRDCYLLEEKERLKEEWSLFKEQKKNFEKERRSFTEAAIRLGLERKAFEEERASWLKQQFLNMTTFDHQNSENMKLFSAFSGSSDRDSLSVHSRPRQKKPHSVSNGTPVCTSKLTKSLPASPSTSDFCQTRSCASEHSSINVLNITPEETKPNQVGREGTNQKWSMASRPGSQEGCYSGCSLTYTNSHVEKDDLP 2ACA58417340B72A she3_yeast SWI5-dependent HO expression protein 3 YBR1005 YBR130C SHE3 protein protein yeast Saccharomyces cerevisiae Baker's yeast MSDQDNTQTSSSKLAPHHNIFMANLESSPTKDRNTSSQNASSSRVIESLHDQIDMLTKTNLQLTTQSQNLLSKLELAQSKESKLLENLNLLKNENENLNSIFERKNKKLKELEKDYSELSNRYNEQKEKMDQLSKLAKNSSAIEQSCSEKLQNMEVNYNSLLESQNLYRDHYSDEISKLNEKIGLLELELSNQNLNYGSDTSSNSDIELNLNKFNDSVKDLKSLETEKDSKLSKIITHSLDELNLQSWLNLYQTNENLISTFAEKMDLKDVLKRNDEKISNKGAVVQTLKKNVQTQVESNNADALSSNNAQDMLPIKMVKLRKTPNTNDSSSNGNSSNNKRRSFYTASPLLSSGSIPKSASPVLPGVKRTASVRKPSSSSSKTNVTHNNDPSTSPTISVPPGVTRTVSSTHKKKGNSMVVHGAQS 590404D2E140BB7A 590404D2E140BB7A mllt4-ssx2ip-1 2 19 unspecified role unspecified role bait bait dil domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain The sequence range [511-981] expresses the DIL region [606-983] of rat afadin. 11 unspecified role unspecified role prey prey physical association physical association Text reference (p4106). mllt4-ssx2ip-3 5 18 unspecified role unspecified role prey prey 19 unspecified role unspecified role bait bait dil domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain The sequence range [511-981] expresses the DIL region [606-983] of rat afadin. physical association physical association Text reference (p4106). mllt4-ssx2ip-5 7 19 unspecified role unspecified role bait bait dil domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain The sequence range [511-981] expresses the DIL region [606-983] of Afadin. 18 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 32 34 physical association physical association Fig 2A, 2B. mllt4-ssx2ip-7 7 19 unspecified role unspecified role bait bait dil domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain The sequence range [511-981] expresses the DIL region [606-983] of rat afadin. 11 unspecified role unspecified role prey prey coiled-coil region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 39 37 physical association physical association Fig 2A, 2B. ssx2ip-mllt4 4 19 unspecified role unspecified role prey prey n-terminal region t7 tag t7 tag MASMTGGQQMG epitope tag MASMTGGQQMG epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range dil domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 11 unspecified role unspecified role bait bait n-terminal region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range c-terminal region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain The C-terminal region [339-615] contains within it the third coiled-coil region of mouse ADIP. 43 46 physical association physical association Fig 3A. ssx2ip-afadin 6 13 unspecified role unspecified role prey prey canlf-mdck Canis familiaris (dog) kidney epithelial cells Canis lupus familiaris Dog Canis familiaris mdck Canine Cocker Spaniel kidney epithelial cell line 11 unspecified role unspecified role bait bait region mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) physical association physical association Fig 3B. ssx2ip-mllt4-1 3 19 unspecified role unspecified role prey prey region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined dil domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 11 unspecified role unspecified role bait bait region mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined coiled-coil domains sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain The sequence [121-436] contains within it all the coiled coil domains of mouse ADIP. ecoli Escherichia coli (strain K12) 57 54 direct interaction direct interaction Fig 3C. ssx2ip-afadin-1 9 13 unspecified role unspecified role prey prey 20 unspecified role unspecified role bait bait physical association physical association Fig 3D. afadin-ssx2ip 1 13 unspecified role unspecified role neutral component neutral component 20 unspecified role unspecified role neutral component neutral component colocalization colocalization Fig 5A. ssx2ip-actn1 5 11 unspecified role unspecified role bait bait coiled-coil domains sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain The sequence [152-436] contains within it all the coiled-coil domains of ADIP. 14 unspecified role unspecified role prey prey c-terminal region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 66 68 physical association physical association Text reference (p 4108). ssx2ip-actn1-1 7 11 unspecified role unspecified role bait bait coiled-coil region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain The sequence [1-226] contains within it the first coiled-coil region of ADIP. 14 unspecified role unspecified role prey prey physical association physical association Fig 2A. ssx2ip-actn1-2 4 12 unspecified role unspecified role prey prey n-terminal region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range c-terminal region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 11 unspecified role unspecified role bait bait coiled-coil domains sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain The sequence [152-436] contains within it all the coiled-coil domains of mouse ADIP. n-terminal region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range 78 76 physical association physical association Fig 8A. ssx2ip-actn1-3 10 11 unspecified role unspecified role bait bait coiled-coil domains sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain The sequence [152-436] contained within it all the coiled-coil domains of mouse ADIP. n-terminal region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range 12 unspecified role unspecified role prey prey physical association physical association Fig 8B. ssx2ip-actn1-4 3 12 unspecified role unspecified role prey prey c-terminal region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 11 unspecified role unspecified role bait bait region mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined coiled-coil domains sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain The sequence [152-436] contains within it all the coiled-coil domains of mouse ADIP. ecoli Escherichia coli (strain K12) 87 91 direct interaction direct interaction Fig 8C. ssx2ip-actn1-5 9 15 unspecified role unspecified role prey prey 20 unspecified role unspecified role bait bait physical association physical association Fig 8D. myo4-she3-1 7 16 unspecified role unspecified role bait bait dil domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain The sequence range for the DIL domain [1164-1419] was inferred from UniProt. 21 unspecified role unspecified role prey prey physical association physical association Fig 2B. mllt4-ssx2ip-8 8 17 unspecified role unspecified role neutral component neutral component 11 unspecified role unspecified role neutral component neutral component colocalization colocalization Fig 5B, 5C.