bhf-ucl ARH is a modular adaptor protein that interacts with the LDL receptor, clathrin, and AP-2. ARH is a modular adaptor protein that interacts with the LDL receptor, clathrin, and AP-2. J. Biol. Chem. (0021-9258) 2002 imex curation He G., Gupta S., Yi M., Michaely P., Hobbs H.H., Cohen J.C. Cardiac - Interactions involving cardiac related proteins fig 6 was not annotated as the purified bovine clathrin represents a complex of several proteins. The experiment does not define a single protein. The sources of proteins are identified as follows: LDLR and ARH are human as stated by author under experimental procedures and in legend of figure 2. Clathrin is bovine as stated by author under experimental procedures. Beta2-adaptin is human as stated by author. Beta2-adaptin is human as stated by author. jonathan.cohen@utsouthwestern.edu. 13-Jan-2014: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot He G., Gupta S., Yi M., Michaely P., Hobbs H.H., Cohen J.C. Cardiac - Interactions involving cardiac related proteins fig 6 was not annotated as the purified bovine clathrin represents a complex of several proteins. The experiment does not define a single protein. The sources of proteins are identified as follows: LDLR and ARH are human as stated by author under experimental procedures and in legend of figure 2. Clathrin is bovine as stated by author under experimental procedures. Beta2-adaptin is human as stated by author. Beta2-adaptin is human as stated by author. jonathan.cohen@utsouthwestern.edu. Accepted 2013-DEC-04 by ORCHARD J. Biol. Chem. (0021-9258) 2002 imex curation ap2b1_human AP-2 complex subunit beta AP2B1 CLAPB1 Beta-2-adaptin Beta-adaptin Plasma membrane adaptor HA2/AP2 adaptin beta subunit Clathrin assembly protein complex 2 beta large chain AP105B Adaptor protein complex AP-2 subunit beta ADTB2 Adaptor-related protein complex 2 subunit beta protein protein human Homo sapiens Human MTDSKYFTTNKKGEIFELKAELNNEKKEKRKEAVKKVIAAMTVGKDVSSLFPDVVNCMQTDNLELKKLVYLYLMNYAKSQPDMAIMAVNSFVKDCEDPNPLIRALAVRTMGCIRVDKITEYLCEPLRKCLKDEDPYVRKTAAVCVAKLHDINAQMVEDQGFLDSLRDLIADSNPMVVANAVAALSEISESHPNSNLLDLNPQNINKLLTALNECTEWGQIFILDCLSNYNPKDDREAQSICERVTPRLSHANSAVVLSAVKVLMKFLELLPKDSDYYNMLLKKLAPPLVTLLSGEPEVQYVALRNINLIVQKRPEILKQEIKVFFVKYNDPIYVKLEKLDIMIRLASQANIAQVLAELKEYATEVDVDFVRKAVRAIGRCAIKVEQSAERCVSTLLDLIQTKVNYVVQEAIVVIRDIFRKYPNKYESIIATLCENLDSLDEPDARAAMIWIVGEYAERIDNADELLESFLEGFHDESTQVQLTLLTAIVKLFLKKPSETQELVQQVLSLATQDSDNPDLRDRGYIYWRLLSTDPVTAKEVVLSEKPLISEETDLIEPTLLDELICHIGSLASVYHKPPNAFVEGSHGIHRKHLPIHHGSTDAGDSPVGTTTATNLEQPQVIPSQGDLLGDLLNLDLGPPVNVPQVSSMQMGAVDLLGGGLDSLVGQSFIPSSVPATFAPSPTPAVVSSGLNDLFELSTGIGMAPGGYVAPKAVWLPAVKAKGLEISGTFTHRQGHIYMEMNFTNKALQHMTDFAIQFNKNSFGVIPSTPLAIHTPLMPNQSIDVSLPLNTLGPVMKMEPLNNLQVAVKNNIDVFYFSCLIPLNVLFVEDGKMERQVFLATWKDIPNENELQFQIKECHLNADTVSSKLQNNNVYTIAKRNVEGQDMLYQSLKLTNGIWILAELRIQPGNPNYTLSLKCRAPEVSQYIYQVYDSILKN DIP protein P63010 original sequence version: 98 DIP protein P63010-1 original sequence version: 109 DIP protein P63010 original sequence version: 129 DIP protein P63010 original sequence version: 134 B472EE5B2AE176DF ldlr_human Low-density lipoprotein receptor LDLR protein protein human Homo sapiens Human MGPWGWKLRWTVALLLAAAGTAVGDRCERNEFQCQDGKCISYKWVCDGSAECQDGSDESQETCLSVTCKSGDFSCGGRVNRCIPQFWRCDGQVDCDNGSDEQGCPPKTCSQDEFRCHDGKCISRQFVCDSDRDCLDGSDEASCPVLTCGPASFQCNSSTCIPQLWACDNDPDCEDGSDEWPQRCRGLYVFQGDSSPCSAFEFHCLSGECIHSSWRCDGGPDCKDKSDEENCAVATCRPDEFQCSDGNCIHGSRQCDREYDCKDMSDEVGCVNVTLCEGPNKFKCHSGECITLDKVCNMARDCRDWSDEPIKECGTNECLDNNGGCSHVCNDLKIGYECLCPDGFQLVAQRRCEDIDECQDPDTCSQLCVNLEGGYKCQCEEGFQLDPHTKACKAVGSIAYLFFTNRHEVRKMTLDRSEYTSLIPNLRNVVALDTEVASNRIYWSDLSQRMICSTQLDRAHGVSSYDTVISRDIQAPDGLAVDWIHSNIYWTDSVLGTVSVADTKGVKRKTLFRENGSKPRAIVVDPVHGFMYWTDWGTPAKIKKGGLNGVDIYSLVTENIQWPNGITLDLLSGRLYWVDSKLHSISSIDVNGGNRKTILEDEKRLAHPFSLAVFEDKVFWTDIINEAIFSANRLTGSDVNLLAENLLSPEDMVLFHNLTQPRGVNWCERTTLSNGGCQYLCLPAPQINPHSPKFTCACPDGMLLARDMRSCLTEAEAAVATQETSTVRLKVSSTAVRTQHTTTRPVPDTSRLPGATPGLTTVEIVTMSHQALGDVAGRGNEKKPSSVRALSIVLPIVLLVFLCLGVFLLWKNWRLKNINSINFDNPVYQKTTEDEVHICHNQDGYSYPSRQMVSLEDDVA DIP protein P01130 original sequence version: 115 DIP protein P01130 original sequence version: 122 DIP protein P01130 original sequence version: 140 DIP protein P01130 original sequence version: 173 A4C28E9B8BADAD5E clh1_bovin Clathrin heavy chain 1 CLTC protein protein bovin Bos taurus (Bovine) Bovine MAQILPIRFQEHLQLQNLGINPANIGFSTLTMESDKFICIREKVGEQAQVVIIDMNDPSNPIRRPISADSAIMNPASKVIALKAGKTLQIFNIEMKSKMKAHTMTDDVTFWKWISLNTVALVTDNAVYHWSMEGESQPVKMFDRHSSLAGCQIINYRTDAKQKWLLLTGISAQQNRVVGAMQLYSVDRKVSQPIEGHAASFAQFKMEGNAEESTLFCFAVRGQAGGKLHIIEVGTPPTGNQPFPKKAVDVFFPPEAQNDFPVAMQISEKHDVVFLITKYGYIHLYDLETGTCIYMNRISGETIFVTAPHEATAGIIGVNRKGQVLSVCVEEENIIPYITNVLQNPDLALRMAVRNNLAGAEELFARKFNALFAQGNYSEAAKVAANAPKGILRTPDTIRRFQSVPAQPGQTSPLLQYFGILLDQGQLNKYESLELCRPVLQQGRKQLLEKWLKEDKLECSEELGDLVKSVDPTLALSVYLRANVPNKVIQCFAETGQVQKIVLYAKKVGYTPDWIFLLRNVMRISPDQGQQFAQMLVQDEEPLADITQIVDVFMEYNLIQQCTAFLLDALKNNRPSEGPLQTRLLEMNLMHAPQVADAILGNQMFTHYDRAHIAQLCEKAGLLQRALEHFTDLYDIKRAVVHTHLLNPEWLVNYFGSLSVEDSLECLRAMLSANIRQNLQICVQVASKYHEQLSTQSLIELFESFKSFEGLFYFLGSIVNFSQDPDVHFKYIQAACKTGQIKEVERICRESNCYDPERVKNFLKEAKLTDQLPLIIVCDRFDFVHDLVLYLYRNNLQKYIEIYVQKVNPSRLPVVIGGLLDVDCSEDVIKNLILVVRGQFSTDELVAEVEKRNRLKLLLPWLEARIHEGCEEPATHNALAKIYIDSNNNPERFLRENPYYDSRVVGKYCEKRDPHLACVAYERGQCDLELINVCNENSLFKSLSRYLVRRKDPELWGSVLLESNPYRRPLIDQVVQTALSETQDPEEVSVTVKAFMTADLPNELIELLEKIVLDNSVFSEHRNLQNLLILTAIKADRTRVMEYINRLDNYDAPDIANIAISNELFEEAFAIFRKFDVNTSAVQVLIEHIGNLDRAYEFAERCNEPAVWSQLAKAQLQKGMVKEAIDSYIKADDPSSYMEVVQAANTSGNWEELVKYLQMARKKARESYVETELIFALAKTNRLAELEEFINGPNNAHIQQVGDRCYDEKMYDAAKLLYNNVSNFGRLASTLVHLGEYQAAVDGARKANSTRTWKEVCFACVDGKEFRLAQMCGLHIVVHADELEELINYYQDRGYFEELITMLEAALGLERAHMGMFTELAILYSKFKPQKMREHLELFWSRVNIPKVLRAAEQAHLWAELVFLYDKYEEYDNAIITMMNHPTDAWKEGQFKDIITKVANVELYYRAIQFYLEFKPLLLNDLLMVLSPRLDHTRAVNYFSKVKQLPLVKPYLRSVQNHNNKSVNESLNNLFITEEDYQALRTSIDAYDNFDNISLAQRLEKHELIEFRRIAAYLFKGNNRWKQSVELCKKDSLYKDAMQYASESKDTELAEELLQWFLQEEKRECFGACLFTCYDLLRPDVVLETAWRHNIMDFAMPYFIQVMKEYLTKVDKLDASESLRKEEEQATETQPIVYGQPQLMLTAGPSVAVPPQAPFGYGYTAPAYGQPQPGFGYSM DIP protein P49951 original sequence version: 108 6C4F2D54801579E2 arh_human Low density lipoprotein receptor adapter protein 1 Autosomal recessive hypercholesterolemia protein LDLRAP1 ARH protein protein human Homo sapiens Human MDALKSAGRALIRSPSLAKQSWGGGGRHRKLPENWTDTRETLLEGMLFSLKYLGMTLVEQPKGEELSAAAIKRIVATAKASGKKLQKVTLKVSPRGIILTDNLTNQLIENVSIYRISYCTADKMHDKVFAYIAQSQHNQSLECHAFLCTKRKMAQAVTLTVAQAFKVAFEFWQVSKEEKEKRDKASQEGGDVLGARQDCTPSLKSLVATGNLLDLEETAKAPLSTVSANTTNMDEVPRPQALSGSSVVWELDDGLDEAFSRLAQSRTNPQVLDTGLTAQDMHYAQCLSPVDWDKPDSSGTEQDDLFSF DE83168CB328D2A7 ldlrap1-ldlr 1 5 anti tag western anti tag western blot anti tag western western blot western blot Immuno blot Immuno blot unspecified role unspecified role bait bait purified purified binding region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain Q5SW96:p.Phe165Ala mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain F A Wed May 02 19:28:43 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'phe165ala' Tue Jun 30 20:13:23 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Phe165Ala' Q5SW96:p.Phe165Val mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain F V Wed May 02 14:52:53 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'phe165val' Tue Jun 30 17:34:49 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Phe165Val' his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecobd Escherichia coli (strain B / BL21-DE3) 3 polyclonal western polyclonal antibody western blot polyclonal western western blot western blot Immuno blot Immuno blot unspecified role unspecified role prey prey purified purified P01130:p.Tyr828Cys mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain Y C Wed May 02 18:33:32 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'tyr828cys' Tue Jun 30 17:41:18 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Tyr828Cys' P01130:p.Tyr828Ala mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain Y A Wed May 02 16:46:55 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'tyr828ala' Tue Jun 30 16:35:44 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Tyr828Ala' cytoplasmic tail sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain The cytoplasmic domain is mapped to the amino acids 811- 860 based on the UniProt entry for LDLR under the heading: sequence annotations (features), regions, topological domain. However, this does not correlate with a domain in InterPro yet. gst tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecobd Escherichia coli (strain B / BL21-DE3) direct interaction direct interaction Figure 2B, 2C ldlrap1-cltc-2 1 4 monoclonal western monoclonal antibody western blot monoclonal western western blot western blot Immuno blot Immuno blot unspecified role unspecified role prey prey purified purified binding region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain gst tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecobd Escherichia coli (strain B / BL21-DE3) 5 anti tag western anti tag western blot anti tag western western blot western blot Immuno blot Immuno blot unspecified role unspecified role bait bait purified purified region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain Q5SW96:p.[Asp214Ala;Glu216Ala] mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain E A certain certain sequence position certain certain certain sequence position certain D A Fri Jul 22 11:07:24 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'glu216ala,asp214ala' Wed May 02 20:05:54 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asp214ala,glu216ala' Tue Jun 30 19:15:41 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Asp214Ala;Glu216Ala]' his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Q5SW96:p.Leu212_Leu213delinsAlaAla mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain LL AA Thu Jul 07 12:17:42 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was leu212ala_leu213ala Fri Jul 22 11:03:00 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'leu212ala,leu213ala' Wed May 02 16:48:16 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu_leu212-213ala_ala' Tue Jun 30 15:34:54 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu212_Leu213delinsAlaAla' ecobd Escherichia coli (strain B / BL21-DE3) 19 22 physical association physical association Figure 5 ldlrap1-ap2b1-1 1 2 unspecified role unspecified role prey prey P63010:p.Glu902Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain E A Wed May 02 17:47:23 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu902ala' Tue Jun 30 17:31:33 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu902Ala' P63010:p.Glu849Ala mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain E A Wed May 02 14:54:11 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu849ala' Tue Jun 30 17:39:39 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu849Ala' gst tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined binding region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain Both InterPro domain references together represent the protein sequence used in this experiment. ecobd Escherichia coli (strain B / BL21-DE3) 5 anti tag western anti tag western blot anti tag western western blot western blot Immuno blot Immuno blot unspecified role unspecified role bait bait purified purified binding region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecobd Escherichia coli (strain B / BL21-DE3) 31 33 physical association physical association Figure 7 ldlrap1-ap2b1-3 1 2 unspecified role unspecified role prey prey gst tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined binding region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain Both InterPro domain references together represent the protein sequence used in this experiment. ecobd Escherichia coli (strain B / BL21-DE3) 5 anti tag western anti tag western blot anti tag western western blot western blot Immuno blot Immuno blot unspecified role unspecified role bait bait purified purified binding region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Q5SW96:p.Arg266Ala mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain R A Wed May 02 16:36:23 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg266ala' Tue Jun 30 18:51:18 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg266Ala' ecobd Escherichia coli (strain B / BL21-DE3) 38 40 physical association physical association Figure 8 C