HPIDb Host Pathogen Interaction database http://www.agbase.msstate.edu/hpi/main.html Multiple signaling interactions of Abl and Arg kinases with the EphB2 receptor. Multiple signaling interactions of Abl and Arg kinases with the EphB2 receptor. Oncogene (0950-9232) 2001 imex curation Yu H.H., Zisch A.H., Dodelet V.C., Pasquale E.B. elenap@burnham.org 17-Jun-2013: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Yu H.H., Zisch A.H., Dodelet V.C., Pasquale E.B. elenap@burnham.org Accepted 2013-MAY-31 by ORCHARD 17-Jun-2013: Contacted by IntAct-Help. Oncogene (0950-9232) 2001 imex curation Multiple signaling interactions of Abl and Arg kinases with the EphB2 receptor. Multiple signaling interactions of Abl and Arg kinases with the EphB2 receptor. Oncogene (0950-9232) 2001 imex curation Yu H.H., Zisch A.H., Dodelet V.C., Pasquale E.B. elenap@burnham.org 17-Jun-2013: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Yu H.H., Zisch A.H., Dodelet V.C., Pasquale E.B. elenap@burnham.org Accepted 2013-MAY-31 by ORCHARD 17-Jun-2013: Contacted by IntAct-Help. Oncogene (0950-9232) 2001 imex curation Multiple signaling interactions of Abl and Arg kinases with the EphB2 receptor. Multiple signaling interactions of Abl and Arg kinases with the EphB2 receptor. Oncogene (0950-9232) 2001 imex curation Yu H.H., Zisch A.H., Dodelet V.C., Pasquale E.B. elenap@burnham.org 17-Jun-2013: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Yu H.H., Zisch A.H., Dodelet V.C., Pasquale E.B. elenap@burnham.org Accepted 2013-MAY-31 by ORCHARD 17-Jun-2013: Contacted by IntAct-Help. Oncogene (0950-9232) 2001 imex curation Multiple signaling interactions of Abl and Arg kinases with the EphB2 receptor. Multiple signaling interactions of Abl and Arg kinases with the EphB2 receptor. Oncogene (0950-9232) 2001 imex curation Yu H.H., Zisch A.H., Dodelet V.C., Pasquale E.B. elenap@burnham.org 17-Jun-2013: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Yu H.H., Zisch A.H., Dodelet V.C., Pasquale E.B. elenap@burnham.org Accepted 2013-MAY-31 by ORCHARD 17-Jun-2013: Contacted by IntAct-Help. Oncogene (0950-9232) 2001 imex curation Multiple signaling interactions of Abl and Arg kinases with the EphB2 receptor. Multiple signaling interactions of Abl and Arg kinases with the EphB2 receptor. Oncogene (0950-9232) 2001 imex curation Yu H.H., Zisch A.H., Dodelet V.C., Pasquale E.B. elenap@burnham.org 17-Jun-2013: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti bait coip anti bait coimmunoprecipitation anti bait coip anti tag western anti tag western blot anti tag western Yu H.H., Zisch A.H., Dodelet V.C., Pasquale E.B. elenap@burnham.org Accepted 2013-MAY-31 by ORCHARD 17-Jun-2013: Contacted by IntAct-Help. Oncogene (0950-9232) 2001 imex curation Multiple signaling interactions of Abl and Arg kinases with the EphB2 receptor. Multiple signaling interactions of Abl and Arg kinases with the EphB2 receptor. Oncogene (0950-9232) 2001 imex curation Yu H.H., Zisch A.H., Dodelet V.C., Pasquale E.B. elenap@burnham.org 17-Jun-2013: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Yu H.H., Zisch A.H., Dodelet V.C., Pasquale E.B. elenap@burnham.org Accepted 2013-MAY-31 by ORCHARD 17-Jun-2013: Contacted by IntAct-Help. Oncogene (0950-9232) 2001 imex curation abl1_mouse Tyrosine-protein kinase ABL1 Abl1 Abl Abelson murine leukemia viral oncogene homolog 1 Proto-oncogene c-Abl p150 Abelson tyrosine-protein kinase 1 protein protein mouse Mus musculus Mouse MLEICLKLVGCKSKKGLSSSSSCYLEEALQRPVASDFEPQGLSEAARWNSKENLLAGPSENDPNLFVALYDFVASGDNTLSITKGEKLRVLGYNHNGEWCEAQTKNGQGWVPSNYITPVNSLEKHSWYHGPVSRNAAEYLLSSGINGSFLVRESESSPGQRSISLRYEGRVYHYRINTASDGKLYVSSESRFNTLAELVHHHSTVADGLITTLHYPAPKRNKPTIYGVSPNYDKWEMERTDITMKHKLGGGQYGEVYEGVWKKYSLTVAVKTLKEDTMEVEEFLKEAAVMKEIKHPNLVQLLGVCTREPPFYIITEFMTYGNLLDYLRECNRQEVSAVVLLYMATQISSAMEYLEKKNFIHRDLAARNCLVGENHLVKVADFGLSRLMTGDTYTAHAGAKFPIKWTAPESLAYNKFSIKSDVWAFGVLLWEIATYGMSPYPGIDLSQVYELLEKDYRMERPEGCPEKVYELMRACWQWNPSDRPSFAEIHQAFETMFQESSISDEVEKELGKRGTRGGAGSMLQAPELPTKTRTCRRAAEQKDAPDTPELLHTKGLGESDALDSEPAVSPLLPRKERGPPDGSLNEDERLLPRDRKTNLFSALIKKKKKMAPTPPKRSSSFREMDGQPDRRGASEDDSRELCNGPPALTSDAAEPTKSPKASNGAGVPNGAFREPGNSGFRSPHMWKKSSTLTGSRLAAAEEESGMSSSKRFLRSCSASCMPHGARDTEWRSVTLPRDLPSAGKQFDSSTFGGHKSEKPALPRKRTSESRSEQVAKSTAMPPPRLVKKNEEAAEEGFKDTESSPGSSPPSLTPKLLRRQVTASPSSGLSHKEEATKGSASGMGTPATAEPAPPSNKVGLSKASSEEMRVRRHKHSSESPGRDKGRLAKLKPAPPPPPACTGKAGKPAQSPSQEAGEAGGPTKTKCTSLAMDAVNTDPTKAGPPGEGLRKPVPPSVPKPQSTAKPPGTPTSPVSTPSTAPAPSPLAGDQQPSSAAFIPLISTRVSLRKTRQPPERIASGTITKGVVLDSTEALCLAISRNSEQMASHSAVLEAGKNLYTFCVSYVDSIQQMRNKFAFREAINKLESNLRELQICPATASSGPAATQDFSKLLSSVKEISDIVRR BD48ADE8557AE95C ephb2_chick Ephrin type-B receptor 2 EPHB2 CEK5 EPH-like kinase 5 protein protein chick Gallus gallus (Chicken) Chicken MGPLWFCCLPLALLPLLAAVEETLMDSTTATAELGWMVHPPSGWEEVSGYDENMNTIRTYQVCNVFESSQNNWLRTKYIRRRGAHRIHVEMKFSVRDCSSIPNVPGSCKETFNLYYYESDFDSATKTFPNWMENPWMKVDTIAADESFSQVDLGGRVMKINTEVRSFGPVSKNGFYLAFQDYGGCMSLIAVRVFYRKCPRVIQNGAVFQETLSGAESTSLVAARGTCISNAEEVDVPIKLYCNGDGEWLVPIGRCMCRPGYESVENGTVCRGCPSGTFKASQGDEGCVHCPINSRTTSEGATNCVCRNGYYRADADPVDMPCTTIPSAPQAVISSVNETSLMLEWTPPRDSGGREDLVYNIICKSCGSGRGACTRCGDNVQFAPRQLGLTEPRIYISDLLAHTQYTFEIQAVNGVTDQSPFSPQFASVNITTNQAAPSAVSIMHQVSRTVDSITLSWSQPDQPNGVILDYELQYYEKNLSELNSTAVKSPTNTVTVQNLKAGTIYVFQVRARTVAGYGRYSGKMYFQTMTEAEYQTSVQEKLPLIIGSSAAGLVFLIAVVVIIIVCNRRRGFERADSEYTDKLQHYTSGHSTYRGPPPGLGVRLFVMTPGMKIYIDPFTYEDPNEAVREFAKEIDISCVKIEQVIGAGEFGEVCSGHLKLPGKREIFVAIKTLKSGYTEKQRRDFLSEASIMGQFDHPNVIHLEGVVTKSSPVMIITEFMENGSLDSFLRQNDGQFTVIQLVGMLRGIAAGMKYLADMNYVHRDLAARNILVNSNLVCKVSDFGLSRFLEDDTSDPTYTSALGGKIPIRWTAPEAIQYRKFTSASDVWSYGIVMWEVMSYGERPYWDMTNQDVINAIEQDYRLPPPMDCPNALHQLMLDCWQKDRNHRPKFGQIVNTLDKMIRNPNSLKAMAPLSSGVNLPLLDRTIPDYTSFNTVDEWLDAIKMSQYKESFASAGFTTFDIVSQMTVEDILRVGVTLAGHQKKILNSIQVMRAQMNQIQSVEV 8D26213970ECC6E0 ephb2_mouse Ephrin type-B receptor 2 Tyrosine-protein kinase receptor EPH-3 Neural kinase Nuk receptor tyrosine kinase Tyrosine-protein kinase receptor SEK-3 Ephb2 Epth3 Nuk Sek3 protein protein mouse Mus musculus Mouse MAVRRLGAALLLLPLLAAVEETLMDSTTATAELGWMVHPPSGWEEVSGYDENMNTIRTYQVCNVFESSQNNWLRTKFIRRRGAHRIHVEMKFSVRDCSSIPSVPGSCKETFNLYYYEADFDLATKTFPNWMENPWVKVDTIAADESFSQVDLGGRVMKINTEVRSFGPVSRNGFYLAFQDYGGCMSLIAVRVFYRKCPRIIQNGAIFQETLSGAESTSLVAARGSCIANAEEVDVPIKLYCNGDGEWLVPIGRCMCKAGFEAVENGTVCRGCPSGTFKANQGDEACTHCPINSRTTSEGATNCVCRNGYYRADLDPLDMPCTTIPSAPQAVISSVNETSLMLEWTPPRDSGGREDLVYNIICKSCGSGRGACTRCGDNVQYAPRQLGLTEPRIYISDLLAHTQYTFEIQAVNGVTDQSPFSPQFASVNITTNQAAPSAVSIMHQVSRTVDSITLSWSQPDQPNGVILDYELQYYEKELSEYNATAIKSPTNTVTVQGLKAGAIYVFQVRARTVAGYGRYSGKMYFQTMTEAEYQTSIKEKLPLIVGSSAAGLVFLIAVVVIAIVCNRRGFERADSEYTDKLQHYTSGHMTPGMKIYIDPFTYEDPNEAVREFAKEIDISCVKIEQVIGAGEFGEVCSGHLKLPGKREIFVAIKTLKSGYTEKQRRDFLSEASIMGQFDHPNVIHLEGVVTKSTPVMIITEFMENGSLDSFLRQNDGQFTVIQLVGMLRGIAAGMKYLADMNYVHRDLAARNILVNSNLVCKVSDFGLSRFLEDDTSDPTYTSALGGKIPIRWTAPEAIQYRKFTSASDVWSYGIVMWEVMSYGERPYWDMTNQDVINAIEQDYRLPPPMDCPSALHQLMLDCWQKDRNHRPKFGQIVNTLDKMIRNPNSLKAMAPLSSGINLPLLDRTIPDYTSFNTVDEWLEAIKMGQYKESFANAGFTSFDVVSQMMMEDILRVGVTLAGHQKKILNSIQVMRAQMNQIQSVEV DIP protein P54763 original sequence version: 151 DIP protein P54763 original sequence version: 119 DIP protein P54763 original sequence version: 123 92DB5234F18758A7 epha3_chick Ephrin type-A receptor 3 EPHA3 CEK4 ETK1 EPH-like kinase 4 Tyrosine-protein kinase receptor ETK1 protein protein chick Gallus gallus (Chicken) Chicken MDRRRLPLLLLCAALGSAGRLSARPGNEVNLLDSKTIQGELGWISYPSHGWEEISGVDEHYTPIRTYQESNVMDHSQNNWLRTNWIPRNSAQKIYVELKFTLRDCNSIPLVLGTCKETFNLYYMESDDDHLAKFREHQFTKIDTIAADESFTQMDLGDRILKLNTEVREVGPVSKKGFYLAFQDVGACVALVSVRVYFKKCPFTVKNLAMFPDTVPMDSQSLVEVRGSCVNHSKEEEPPKMYCSTEGEWLVPIGKCLCNAGYEERGFACQACRPGFYKASAGNVKCAKCPPHSSTYEDASLNCRCEKNYFRSEKDPPSMACTRPPSAPRNVISNINETSVILDWSWPLDTGGRKDVTFNIICKKCGGSSKICEPCSDNVRFLPRQTGLTNTTVTVVDLLAHTNYTFEIDAVNGVSDLSTLSRQFAAVSITTNQAAPSPITVIRKDRTSRNSVSLSWQEPEHPNGIILDYEVKYYEKQEQETSYTILRAKSTNVTISGLKPDTTYVFQIRARTAARYGTSSRKFEFETSPDSFSISSENSQVVMIAISAAVAIILLTVVVYVLIGRFCGYKKSKHGTDEKRLHFGNGHLKLPGLRTYVDPHTYEDPNQAVHEFAKELDASNISIDKVVGAGEFGEVCSGRLKLPSKKEISVAIKTLKAGYTEKQRRDFLGEASIMGQFDHPNIIRLEGVVTKSKPVMIVTEYMENGSLDSFLRKHDAQFTVIQLVGMLRGIASGMKYLSDMGYVHRDLAARNILINSNLVCKVSDFGLSRVLEDDPEAAYTTRGGKIPIRWTSPEAIAYRKFTSASDAWSYGIVLWEVMSYGERPYWEMSFQDVIKAVDEGYRLPPPMDCPAALYQLMLDCWQKDRNNRPKFEQIVSILDKLIRNPSSLKIITNAAARPSNLLLDQSNIDISAFRTAGDWLNGFRTGQCKGIFTGVEYSSCDTIAKISTDDMKKVGVTVVGPQKKIVSSIKTLETHTKNSPVPV E8895F0BDF77651E abl2_mouse Tyrosine-protein kinase ABL2 Abl2 Arg Abelson murine leukemia viral oncogene homolog 2 Abelson-related gene protein Tyrosine-protein kinase ARG Abelson tyrosine-protein kinase 2 protein protein mouse Mus musculus Mouse MGQQVGRVGEAPGLQQPQPRGIRGSSAARPSGRRRDPAGRTADAGFNVFTQHDHFASCVEDGFEGDKTGGSSPEVLHRPFGCDAESQALNEAIRWSSKENLLGATESDPNLFVALYDFVASGDNTLSITKGEKLRVLGYNQNGEWSEVRSKNGQGWVPSNYITPVNSLEKHSWYHGPVSRSAAEYLLSSLINGSFLVRESESSPGQLSISLRYEGRVYHYRINTTTDSKVYVTAESRFSTLAELVHHHSTVADGLVTTLHYPAPKCNKPTVYGVSPIHDKWEMERTDITMKHKLGGGQYGEVYVGVWKKYSLTVAVKTFKEDTMEVEEFLKEAAVMKEIKHPNLVQLLGVCTLEPPFYIVTEYMPYGNLLDYLRECSREEVTAVVLLYMATQISSAMEYLEKKNFIHRDLAARNCLVGENHVVKVADFGLSRLMTGDTYTAHAGAKFPIKWTAPESLAYNTFSIKSDVWAFGVLLWEIATYGMSPYPGIDLSQVYDLLEKGYRMEQPEGCPPKVYELMRACWKWSPADRPSFAETHQAFETMFHDSSISEEVAEELGRTASSSSVVPYLPRLPLLPSKTRTLRKQGENKENLDGGLDAAESLASSSAPAGFIRSTQASSGSPALPRKQRDKSPSSLLEDAKETCFTRDRKGGFFSSFMKKRNAPTPPKRSSSFREMENQPHKKYELTGNFSPVASLQNADGFSVAPSQQEPNLVPAKCYGGSFAQRNLCADDDSGGGGGSGTAGGGWSGITGFFTPRLIKKTLGLRAGKPTASDDTSKPFPRSNSTSSMSSGLPEQDRMAMTLPRNCQRSKLQLERTVSTSSQPEENVDRANDMLPKKSEEGAAPARERPKAKLLPRGATALPLRAPDPAITESDSPGVGVAGVAAAPKGKERNGGTRLGVAGVPEDGEQLGWSSPAKAVAVLPTTHNHKVPVLISPTLKHTPADVQLIGTDSQGNKFKLLSEHQVTSSGDKDRPRRVKPKCAPPPPPVMRLLQHPSTCSDPEEEPTAPPAGQHTPETQEGGKKAAPGPVPSSGKPGRPVMPPPQVPLPTSSISPAKMANGTAGTKVALRKTKQAAEKISADKISKEALLECADLLSSAITEPVPNSQLVDTGHQLLDYCSGYVDSIPQTRNKFAFREAVSKLELSLQELQVSSTAAGVPGTNPVLNNLLSCVQEISDVVQR DIP protein Q4JIM5 original sequence version: 83 08507298A9081228 f1nhc7_chick Receptor protein-tyrosine kinase EPHB2 protein protein chick Gallus gallus (Chicken) Chicken MPGPERTMGPLWFCCLPLALLPLLAAVEETLMDSTTATAELGWMVHPPSGWEEVSGYDENMNTIRTYQVCNVFESSQNNWLRTKYIRRRGAHRIHVEMKFSVRDCSSIPNVPGSCKETFNLYYYESDFDSATKTFPNWMENPWMKVDTIAADESFSQVDLGGRVMKINTEVRSFGPVSKNGFYLAFQDYGGCMSLIAVRVFYRKCPRVIQNGAVFQETLSGAESTSLVAARGTCISNAEEVDVPIKLYCNGDGEWLVPIGRCMCRPGYESVENGTVCRGCPSGTFKASQGDEGCVHCPINSRTTSEGATNCVCRNGYYRADADPVDMPCTTIPSAPQAVISSVNETSLMLEWTPPRDSGGREDLVYNIICKSCGSGRGACTRCGDNVQFAPRQLGLTEPRIYISDLLAHTQYTFEIQAVNGVTDQSPFSPQFASVNITTNQAAPSAVSIMHQVSRTVDSITLSWSQPDQPNGVILDYELQYYEKNLSELNSTAVKSPTNTVTVQNLKAGTIYVFQVRARTVAGYGRYSGKMYFQTMTEAEYQTSVQEKLPLIIGSSAAGLVFLIAVVVIIIVCNRRRGFERADSEYTDKLQHYTSGHMTPGMKIYIDPFTYEDPNEAVREFAKEIDISCVKIEQVIGAGEFGEVCSGHLKLPGKREIFVAIKTLKSGYTEKQRRDFLSEASIMGQFDHPNVIHLEGVVTKSSPVMIITEFMENGSLDSFLRQNDGQFTVIQLVGMLRGIAAGMKYLADMNYVHRDLAARNILVNSNLVCKVSDFGLSRFLEDDTSDPTYTSALGGKIPIRWTAPEAIQYRKFTSASDVWSYGIVMWEVMSYGERPYWDMTNQDVINAIEQDYRLPPPMDCPNALHQLMLDCWQKDRNHRPKFGQIVNTLDKMIRNPNSLKAMAPLSSGVNLPLLDRTIPDYTSFNTVDEWLDAIKMSQYKESFASAGFTTFDIVSQMTVEDILRVGVTLAGHQKKILNSIQVMRAQMNQIQSVEV D071AA981EA268A3 epha4_mouse Ephrin type-A receptor 4 Tyrosine-protein kinase receptor SEK-1 Tyrosine-protein kinase receptor MPK-3 Epha4 Sek Sek1 protein protein mouse Mus musculus Mouse MAGIFYFILFSFLFGICDAVTGSRVYPANEVTLLDSRSVQGELGWIASPLEGGWEEVSIMDEKNTPIRTYQVCNVMEASQNNWLRTDWITREGAQRVYIEIKFTLRDCNSLPGVMGTCKETFNLYYYESDNDKERFIRESQFGKIDTIAADESFTQVDIGDRIMKLNTEIRDVGPLSKKGFYLAFQDVGACIALVSVRVFYKKCPLTVRNLAQFPDTITGADTSSLVEVRGSCVNNSEEKDVPKMYCGADGEWLVPIGNCLCNAGHEEQNGECQACKIGYYKALSTDASCAKCPPHSYSVWEGATSCTCDRGFFRADNDAASMPCTRPPSAPLNLISNVNETSVNLEWSSPQNTGGRQDISYNVVCKKCGAGDPSKCRPCGSGVHYTPQQNGLKTTRVSITDLLAHTNYTFEIWAVNGVSKYNPSPDQSVSVTVTTNQAAPSSIALVQAKEVTRYSVALAWLEPDRPNGVILEYEVKYYEKDQNERSYRIVRTAARNTDIKGLNPLTSYVFHVRARTAAGYGDFSEPLEVTTNTVPSRIIGDGANSTVLLVSVSGSVVLVVILIAAFVISRRRSKYSKAKQEADEEKHLNQGVRTYVDPFTYEDPNQAVREFAKEIDASCIKIEKVIGVGEFGEVCSGRLKVPGKREICVAIKTLKAGYTDKQRRDFLSEASIMGQFDHPNIIHLEGVVTKCKPVMIITEYMENGSLDAFLRKNDGRFTVIQLVGMLRGIGSGMKYLSDMSYVHRDLAARNILVNSNLVCKVSDFGMSRVLEDDPEAAYTTRGGKIPIRWTAPEAIAYRKFTSASDVWSYGIVMWEVMSYGERPYWDMSNQDVIKAIEEGYRLPPPMDCPIALHQLMLDCWQKERSDRPKFGQIVNMLDKLIRNPNSLKRTGSESSRPNTALLDPSSPEFSAVVSVGDWLQAIKMDRYKDNFTAAGYTTLEAVVHMSQDDLARIGITAITHQNKILSSVQAMRTQMQQMHGRMVPV DIP protein Q03137 original sequence version: 161 DIP protein Q03137 original sequence version: 101 89BEB2C7CDB54A55 q98946_chick Non-specific protein-tyrosine kinase ABL protein protein chick Gallus gallus (Chicken) Chicken EDTMEVEEFLKEAAVMKEIKHPNLVQLLGVCTREPPFYIITEFMTYGNLLDYLRECNRQEVNAVVLLYMATQISSAMEYLEKKNFIH 98D825B2E0C482EA abl2_human Tyrosine-protein kinase ABL2 Abelson tyrosine-protein kinase 2 ABL2 ABLL ARG Abelson murine leukemia viral oncogene homolog 2 Abelson-related gene protein Tyrosine-protein kinase ARG protein protein human Homo sapiens Human MGQQVGRVGEAPGLQQPQPRGIRGSSAARPSGRRRDPAGRTTETGFNIFTQHDHFASCVEDGFEGDKTGGSSPEALHRPYGCDVEPQALNEAIRWSSKENLLGATESDPNLFVALYDFVASGDNTLSITKGEKLRVLGYNQNGEWSEVRSKNGQGWVPSNYITPVNSLEKHSWYHGPVSRSAAEYLLSSLINGSFLVRESESSPGQLSISLRYEGRVYHYRINTTADGKVYVTAESRFSTLAELVHHHSTVADGLVTTLHYPAPKCNKPTVYGVSPIHDKWEMERTDITMKHKLGGGQYGEVYVGVWKKYSLTVAVKTLKEDTMEVEEFLKEAAVMKEIKHPNLVQLLGVCTLEPPFYIVTEYMPYGNLLDYLRECNREEVTAVVLLYMATQISSAMEYLEKKNFIHRDLAARNCLVGENHVVKVADFGLSRLMTGDTYTAHAGAKFPIKWTAPESLAYNTFSIKSDVWAFGVLLWEIATYGMSPYPGIDLSQVYDLLEKGYRMEQPEGCPPKVYELMRACWKWSPADRPSFAETHQAFETMFHDSSISEEVAEELGRAASSSSVVPYLPRLPILPSKTRTLKKQVENKENIEGAQDATENSASSLAPGFIRGAQASSGSPALPRKQRDKSPSSLLEDAKETCFTRDRKGGFFSSFMKKRNAPTPPKRSSSFREMENQPHKKYELTGNFSSVASLQHADGFSFTPAQQEANLVPPKCYGGSFAQRNLCNDDGGGGGGSGTAGGGWSGITGFFTPRLIKKTLGLRAGKPTASDDTSKPFPRSNSTSSMSSGLPEQDRMAMTLPRNCQRSKLQLERTVSTSSQPEENVDRANDMLPKKSEESAAPSRERPKAKLLPRGATALPLRTPSGDLAITEKDPPGVGVAGVAAAPKGKEKNGGARLGMAGVPEDGEQPGWPSPAKAAPVLPTTHNHKVPVLISPTLKHTPADVQLIGTDSQGNKFKLLSEHQVTSSGDKDRPRRVKPKCAPPPPPVMRLLQHPSICSDPTEEPTALTAGQSTSETQEGGKKAALGAVPISGKAGRPVMPPPQVPLPTSSISPAKMANGTAGTKVALRKTKQAAEKISADKISKEALLECADLLSSALTEPVPNSQLVDTGHQLLDYCSGYVDCIPQTRNKFAFREAVSKLELSLQELQVSSAAAGVPGTNPVLNNLLSCVQEISDVVQR ED93869BC2B14FAA ED93869BC2B14FAA ephb2-abl1-2 6 8 unspecified role unspecified role bait bait Abl type 4 7 unspecified role unspecified role prey prey abl sh2 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association table1 ephb2-abl2-2 6 Arg type 1B 11 unspecified role unspecified role prey prey arg c sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 8 unspecified role unspecified role bait bait physical association physical association table1 Arg C-terminal binds to EphB2 is phosphorylation-independent. epha4-abl-1 6 EphA4 13 unspecified role unspecified role bait bait Abl type 4 7 unspecified role unspecified role prey prey abl sh2 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association Table 1 abl1-ephb2-3 3 Abl type 4 7 unspecified role unspecified role bait bait gst-abl sh2 tag tag western blot western blot Immuno blot Immuno blot undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 8 unspecified role unspecified role prey prey P28693:p.[Tyr614Glu;Tyr620Glu] mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain Y E certain certain sequence position certain certain certain sequence position certain Y E Wed May 02 18:02:32 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'tyr614glu,tyr620glu' Tue Jun 30 17:35:48 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Tyr614Glu;Tyr620Glu]' physical association physical association 3A Abl SH2 domain binding to EphB2 require phosphorylation of tyrosines 605 and 611. ephb2-epha3-abl-1 2 EphB2 12 unspecified role unspecified role prey prey human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. Abl type 4 14 unspecified role unspecified role bait bait gst-abl sh2 tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 9bact Bacteria EphA3 10 unspecified role unspecified role prey prey human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. physical association physical association 3B ephb2-abl1-1 1 Abl type 4 7 unspecified role unspecified role prey prey nucl transfection nucleic acid transfection nucl transfection abl1 flag tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged certain certain sequence position certain certain certain sequence position certain 8 unspecified role unspecified role bait bait physical association physical association 4B, 6A Abl C-terminal motif mediates binding to EphB2 in a phosphorylation-independent manner. ephb2-abl2-1 5 Arg type 1B 15 unspecified role unspecified role prey prey nucl transfection nucleic acid transfection nucl transfection tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 8 unspecified role unspecified role bait bait physical association physical association 4C, 5B, 6B Arg C-terminal motif is required for binding to EphB2 independently of EphB2 tyrosine phosphorylation. abl1-ephb2-6 1 Abl type 4 7 unspecified role unspecified role bait bait nucl transfection nucleic acid transfection nucl transfection 8 unspecified role unspecified role prey prey physical association physical association Figs. 4A, 5A ephb2-arg-4 4 Arg type 1B 11 unspecified role unspecified role prey prey arg c sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain EphB2 9 unspecified role unspecified role bait bait P54763:p.Ile597Glu mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain I E Wed May 02 20:20:10 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ile597glu' Tue Jun 30 17:44:16 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ile597Glu' physical association physical association 2 Arg SH2 domain binds to the tyrosine 611 motif of EphB2 (fig 2). ephb2-abl1-3 4 8 unspecified role unspecified role bait bait P28693:p.Tyr614Glu mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain Y E Wed May 02 15:45:36 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'tyr614glu' Tue Jun 30 16:59:50 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Tyr614Glu' P28693:p.Tyr620Glu mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain Y E Wed May 02 17:12:46 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'tyr620glu' Tue Jun 30 17:00:20 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Tyr620Glu' P28693:p.[Tyr614Glu;Tyr620Glu] mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain Y E certain certain sequence position certain certain certain sequence position certain Y E Wed May 02 15:00:01 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'tyr614glu,tyr620glu' Tue Jun 30 17:37:26 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Tyr614Glu;Tyr620Glu]' Abl type 4 7 unspecified role unspecified role prey prey abl sh2 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain physical association physical association 2 Abl SH2 domain binds to both 605 and 611 tyrosine phosphorylated motifs on EphB2.