mbinfo MBInfo http://www.mechanobio.info/ Conformation, localization, and integrin binding of talin depend on its interaction with phosphoinositides. Conformation, localization, and integrin binding of talin depend on its interaction with phosphoinositides. J. Biol. Chem. (0021-9258) 2001 imex curation Martel V., Racaud-Sultan C., Dupe S., Marie C., Paulhe F., Galmiche A., Block M.R., Albiges-Rizo C. Participant detection: The molecules analyzed by SDS-PAGE were detected by coomassie staining and was then subjected to densitometric quantification. The in vivo association of phosphoinositides and talin (shown in Fig 2) could not be curated since the specific talin isoform (1/2) immunoprecipitated could not be identified. No contact-email available. 16-Apr-2013: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro solid phase assay solid phase assay western blot western blot Immuno blot Immuno blot Martel V., Racaud-Sultan C., Dupe S., Marie C., Paulhe F., Galmiche A., Block M.R., Albiges-Rizo C. Participant detection: The molecules analyzed by SDS-PAGE were detected by coomassie staining and was then subjected to densitometric quantification. The in vivo association of phosphoinositides and talin (shown in Fig 2) could not be curated since the specific talin isoform (1/2) immunoprecipitated could not be identified. The in vivo association of phosphoinositides and talin (shown in Fig 2) could not be curated since the specific talin isoform (1/2) immunoprecipitated could not be identified. No contact-email available. Accepted 2013-APR-11 by ORCHARD 16-Apr-2013: Contacted by IntAct-Help. J. Biol. Chem. (0021-9258) 2001 Only protein-protein interactions imex curation Conformation, localization, and integrin binding of talin depend on its interaction with phosphoinositides. Conformation, localization, and integrin binding of talin depend on its interaction with phosphoinositides. J. Biol. Chem. (0021-9258) 2001 imex curation Martel V., Racaud-Sultan C., Dupe S., Marie C., Paulhe F., Galmiche A., Block M.R., Albiges-Rizo C. Participant detection: The molecules analyzed by SDS-PAGE were detected by coomassie staining and was then subjected to densitometric quantification. The in vivo association of phosphoinositides and talin (shown in Fig 2) could not be curated since the specific talin isoform (1/2) immunoprecipitated could not be identified. No contact-email available. 16-Apr-2013: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro liposome binding assay liposome binding assay weight by comassie molecular weight estimation by coomasie staining weight by comassie Martel V., Racaud-Sultan C., Dupe S., Marie C., Paulhe F., Galmiche A., Block M.R., Albiges-Rizo C. Participant detection: The molecules analyzed by SDS-PAGE were detected by coomassie staining and was then subjected to densitometric quantification. The in vivo association of phosphoinositides and talin (shown in Fig 2) could not be curated since the specific talin isoform (1/2) immunoprecipitated could not be identified. No contact-email available. Accepted 2013-APR-11 by ORCHARD 16-Apr-2013: Contacted by IntAct-Help. J. Biol. Chem. (0021-9258) 2001 Only protein-protein interactions imex curation itb1_human Integrin beta-1 Fibronectin receptor subunit beta VLA-4 subunit beta ITGB1 FNRB MDF2 MSK12 Glycoprotein IIa protein protein human Homo sapiens Human MNLQPIFWIGLISSVCCVFAQTDENRCLKANAKSCGECIQAGPNCGWCTNSTFLQEGMPTSARCDDLEALKKKGCPPDDIENPRGSKDIKKNKNVTNRSKGTAEKLKPEDITQIQPQQLVLRLRSGEPQTFTLKFKRAEDYPIDLYYLMDLSYSMKDDLENVKSLGTDLMNEMRRITSDFRIGFGSFVEKTVMPYISTTPAKLRNPCTSEQNCTSPFSYKNVLSLTNKGEVFNELVGKQRISGNLDSPEGGFDAIMQVAVCGSLIGWRNVTRLLVFSTDAGFHFAGDGKLGGIVLPNDGQCHLENNMYTMSHYYDYPSIAHLVQKLSENNIQTIFAVTEEFQPVYKELKNLIPKSAVGTLSANSSNVIQLIIDAYNSLSSEVILENGKLSEGVTISYKSYCKNGVNGTGENGRKCSNISIGDEVQFEISITSNKCPKKDSDSFKIRPLGFTEEVEVILQYICECECQSEGIPESPKCHEGNGTFECGACRCNEGRVGRHCECSTDEVNSEDMDAYCRKENSSEICSNNGECVCGQCVCRKRDNTNEIYSGKFCECDNFNCDRSNGLICGGNGVCKCRVCECNPNYTGSACDCSLDTSTCEASNGQICNGRGICECGVCKCTDPKFQGQTCEMCQTCLGVCAEHKECVQCRAFNKGEKKDTCTQECSYFNITKVESRDKLPQPVQPDPVSHCKEKDVDDCWFYFTYSVNGNNEVMVHVVENPECPTGPDIIPIVAGVVAGIVLIGLALLLIWKLLMIIHDRREFAKFEKEKMNAKWDTGENPIYKSAVTTVVNPKYEGK DIP protein P05556 original sequence version: 139 DIP protein P05556-1 original sequence version: 143 DIP protein P05556 original sequence version: 154 DIP protein P05556 original sequence version: 167 DE35979C1625578C tln1_human Talin-1 TLN1 KIAA1027 TLN protein protein human Homo sapiens Human MVALSLKISIGNVVKTMQFEPSTMVYDACRIIRERIPEAPAGPPSDFGLFLSDDDPKKGIWLEAGKALDYYMLRNGDTMEYRKKQRPLKIRMLDGTVKTIMVDDSKTVTDMLMTICARIGITNHDEYSLVRELMEEKKEEITGTLRKDKTLLRDEKKMEKLKQKLHTDDELNWLDHGRTLREQGVEEHETLLLRRKFFYSDQNVDSRDPVQLNLLYVQARDDILNGSHPVSFDKACEFAGFQCQIQFGPHNEQKHKAGFLDLKDFLPKEYVKQKGERKIFQAHKNCGQMSEIEAKVRYVKLARSLKTYGVSFFLVKEKMKGKNKLVPRLLGITKECVMRVDEKTKEVIQEWNLTNIKRWAASPKSFTLDFGDYQDGYYSVQTTEGEQIAQLIAGYIDIILKKKKSKDHFGLEGDEESTMLEDSVSPKKSTVLQQQYNRVGKVEHGSVALPAIMRSGASGPENFQVGSMPPAQQQITSGQMHRGHMPPLTSAQQALTGTINSSMQAVQAAQATLDDFDTLPPLGQDAASKAWRKNKMDESKHEIHSQVDAITAGTASVVNLTAGDPAETDYTAVGCAVTTISSNLTEMSRGVKLLAALLEDEGGSGRPLLQAAKGLAGAVSELLRSAQPASAEPRQNLLQAAGNVGQASGELLQQIGESDTDPHFQDALMQLAKAVASAAAALVLKAKSVAQRTEDSGLQTQVIAAATQCALSTSQLVACTKVVAPTISSPVCQEQLVEAGRLVAKAVEGCVSASQAATEDGQLLRGVGAAATAVTQALNELLQHVKAHATGAGPAGRYDQATDTILTVTENIFSSMGDAGEMVRQARILAQATSDLVNAIKADAEGESDLENSRKLLSAAKILADATAKMVEAAKGAAAHPDSEEQQQRLREAAEGLRMATNAAAQNAIKKKLVQRLEHAAKQAAASATQTIAAAQHAASTPKASAGPQPLLVQSCKAVAEQIPLLVQGVRGSQAQPDSPSAQLALIAASQSFLQPGGKMVAAAKASVPTIQDQASAMQLSQCAKNLGTALAELRTAAQKAQEACGPLEMDSALSVVQNLEKDLQEVKAAARDGKLKPLPGETMEKCTQDLGNSTKAVSSAIAQLLGEVAQGNENYAGIAARDVAGGLRSLAQAARGVAALTSDPAVQAIVLDTASDVLDKASSLIEEAKKAAGHPGDPESQQRLAQVAKAVTQALNRCVSCLPGQRDVDNALRAVGDASKRLLSDSLPPSTGTFQEAQSRLNEAAAGLNQAATELVQASRGTPQDLARASGRFGQDFSTFLEAGVEMAGQAPSQEDRAQVVSNLKGISMSSSKLLLAAKALSTDPAAPNLKSQLAAAARAVTDSINQLITMCTQQAPGQKECDNALRELETVRELLENPVQPINDMSYFGCLDSVMENSKVLGEAMTGISQNAKNGNLPEFGDAISTASKALCGFTEAAAQAAYLVGVSDPNSQAGQQGLVEPTQFARANQAIQMACQSLGEPGCTQAQVLSAATIVAKHTSALCNSCRLASARTTNPTAKRQFVQSAKEVANSTANLVKTIKALDGAFTEENRAQCRAATAPLLEAVDNLSAFASNPEFSSIPAQISPEGRAAMEPIVISAKTMLESAGGLIQTARALAVNPRDPPSWSVLAGHSRTVSDSIKKLITSMRDKAPGQLECETAIAALNSCLRDLDQASLAAVSQQLAPREGISQEALHTQMLTAVQEISHLIEPLANAARAEASQLGHKVSQMAQYFEPLTLAAVGAASKTLSHPQQMALLDQTKTLAESALQLLYTAKEAGGNPKQAAHTQEALEEAVQMMTEAVEDLTTTLNEAASAAGVVGGMVDSITQAINQLDEGPMGEPEGSFVDYQTTMVRTAKAIAVTVQEMVTKSNTSPEELGPLANQLTSDYGRLASEAKPAAVAAENEEIGSHIKHRVQELGHGCAALVTKAGALQCSPSDAYTKKELIECARRVSEKVSHVLAALQAGNRGTQACITAASAVSGIIADLDTTIMFATAGTLNREGTETFADHREGILKTAKVLVEDTKVLVQNAAGSQEKLAQAAQSSVATITRLADVVKLGAASLGAEDPETQVVLINAVKDVAKALGDLISATKAAAGKVGDDPAVWQLKNSAKVMVTNVTSLLKTVKAVEDEATKGTRALEATTEHIRQELAVFCSPEPPAKTSTPEDFIRMTKGITMATAKAVAAGNSCRQEDVIATANLSRRAIADMLRACKEAAYHPEVAPDVRLRALHYGRECANGYLELLDHVLLTLQKPSPELKQQLTGHSKRVAGSVTELIQAAEAMKGTEWVDPEDPTVIAENELLGAAAAIEAAAKKLEQLKPRAKPKEADESLNFEEQILEAAKSIAAATSALVKAASAAQRELVAQGKVGAIPANALDDGQWSQGLISAARMVAAATNNLCEAANAAVQGHASQEKLISSAKQVAASTAQLLVACKVKADQDSEAMKRLQAAGNAVKRASDNLVKAAQKAAAFEEQENETVVVKEKMVGGIAQIIAAQEEMLRKERELEEARKKLAQIRQQQYKFLPSELRDEH E21575E9199BBC5C pip phosphatidylinositol phosphates small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) phosphatidylinositol phosphatidylinositol 1-phosphatidyl-1d-myo-inositol 4,5-bisphosphate 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphates small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) pip-tln1-1 2 4 unspecified role unspecified role neutral component neutral component human-platelet Homo sapiens platelet, thrombocytes platelet Platelet, thrombocyte 5 unspecified role unspecified role neutral component neutral component direct interaction direct interaction Fig 1A. 1_phosphatidyl_-tln1 2 4 unspecified role unspecified role neutral component neutral component human-platelet Homo sapiens platelet, thrombocytes platelet Platelet, thrombocyte 6 unspecified role unspecified role neutral component neutral component direct interaction direct interaction Fig 1A, 1C. itgb1-tln1-2 1 3 unspecified role unspecified role bait bait region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined cytoplasmic domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 4 unspecified role unspecified role prey prey human-platelet Homo sapiens platelet, thrombocytes platelet Platelet, thrombocyte direct interaction direct interaction Fig 4A. The association of talin with integrin beta-1 cytoplasmic domain was enhanced when talin was preincubated with PIP or PI(4,5)P2. itgb1-tln1-3 1 4 unspecified role unspecified role prey prey human-platelet Homo sapiens platelet, thrombocytes platelet Platelet, thrombocyte 3 unspecified role unspecified role bait bait human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 physical association physical association Fig 5C, 7B. The amount of talin associated with the antibody-bound integrin beta-1 was enhanced when talin was preincubated with PIP or PI(4,5)P2 (Fig 5C). The talin-integrin beta-1 association increased in the presence of increasing concentrations of talin, preincubated with PI(4,5)P2 (Fig 7B).