IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Ca2+ binding properties and Ca2(+)-dependent interactions of the isolated NH2-terminal alpha fragments of human complement proteases C1-r and C1-s. Ca2+ binding properties and Ca2(+)-dependent interactions of the isolated NH2-terminal alpha fragments of human complement proteases C1-r and C1-s. J. Biol. Chem. (0021-9258) 1990 imex curation Thielens N.M., Aude C.A., Lacroix M.B., Gagnon J., Arlaud G.J. I've not included their dimer/tetramer info as i think they have inferred it from previous work - I can't see how they know the stoichiometry from an uncalibrated column. C1S andC1R were purified from human serum by column chromatography and identity plus length of the alpha fragments were established by Edman sequencing and amino acid content. 15-Nov-2012: Contacted by pporras. Only protein-protein interactions in vitro In vitro equilib. dialysis equilibrium dialysis equilib. dialysis predetermined predetermined participant predetermined Thielens N.M., Aude C.A., Lacroix M.B., Gagnon J., Arlaud G.J. I've not included their dimer/tetramer info as i think they have inferred it from previous work - I can't see how they know the stoichiometry from an uncalibrated column. C1S andC1R were purified from human serum by column chromatography and identity plus length of the alpha fragments were established by Edman sequencing and amino acid content. Accepted 2012-NOV-06 by PPORRAS 15-Nov-2012: Contacted by pporras. J. Biol. Chem. (0021-9258) 1990 Only protein-protein interactions imex curation Ca2+ binding properties and Ca2(+)-dependent interactions of the isolated NH2-terminal alpha fragments of human complement proteases C1-r and C1-s. Ca2+ binding properties and Ca2(+)-dependent interactions of the isolated NH2-terminal alpha fragments of human complement proteases C1-r and C1-s. J. Biol. Chem. (0021-9258) 1990 imex curation Thielens N.M., Aude C.A., Lacroix M.B., Gagnon J., Arlaud G.J. I've not included their dimer/tetramer info as i think they have inferred it from previous work - I can't see how they know the stoichiometry from an uncalibrated column. C1S andC1R were purified from human serum by column chromatography and identity plus length of the alpha fragments were established by Edman sequencing and amino acid content. 15-Nov-2012: Contacted by pporras. Only protein-protein interactions in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Thielens N.M., Aude C.A., Lacroix M.B., Gagnon J., Arlaud G.J. I've not included their dimer/tetramer info as i think they have inferred it from previous work - I can't see how they know the stoichiometry from an uncalibrated column. C1S andC1R were purified from human serum by column chromatography and identity plus length of the alpha fragments were established by Edman sequencing and amino acid content. Accepted 2012-NOV-06 by PPORRAS 15-Nov-2012: Contacted by pporras. J. Biol. Chem. (0021-9258) 1990 Only protein-protein interactions imex curation c1r_human Complement C1r subcomponent C1R Complement component 1 subcomponent r protein protein human Homo sapiens Human MWLLYLLVPALFCRAGGSIPIPQKLFGEVTSPLFPKPYPNNFETTTVITVPTGYRVKLVFQQFDLEPSEGCFYDYVKISADKKSLGRFCGQLGSPLGNPPGKKEFMSQGNKMLLTFHTDFSNEENGTIMFYKGFLAYYQAVDLDECASRSKSGEEDPQPQCQHLCHNYVGGYFCSCRPGYELQEDTHSCQAECSSELYTEASGYISSLEYPRSYPPDLRCNYSIRVERGLTLHLKFLEPFDIDDHQQVHCPYDQLQIYANGKNIGEFCGKQRPPDLDTSSNAVDLLFFTDESGDSRGWKLRYTTEIIKCPQPKTLDEFTIIQNLQPQYQFRDYFIATCKQGYQLIEGNQVLHSFTAVCQDDGTWHRAMPRCKIKDCGQPRNLPNGDFRYTTTMGVNTYKARIQYYCHEPYYKMQTRAGSRESEQGVYTCTAQGIWKNEQKGEKIPRCLPVCGKPVNPVEQRQRIIGGQKAKMGNFPWQVFTNIHGRGGGALLGDRWILTAAHTLYPKEHEAQSNASLDVFLGHTNVEELMKLGNHPIRRVSVHPDYRQDESYNFEGDIALLELENSVTLGPNLLPICLPDNDTFYDLGLMGYVSGFGVMEEKIAHDLRFVRLPVANPQACENWLRGKNRMDVFSQNMFCAGHPSLKQDACQGDSGGVFAVRDPNTDRWVATGIVSWGIGCSRGYGFYTKVLNYVDWIKKEMEEED B45D120201061462 calcium(2+) small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) c1s_human Complement C1s subcomponent C1S C1 esterase Complement component 1 subcomponent s protein protein human Homo sapiens Human MWCIVLFSLLAWVYAEPTMYGEILSPNYPQAYPSEVEKSWDIEVPEGYGIHLYFTHLDIELSENCAYDSVQIISGDTEEGRLCGQRSSNNPHSPIVEEFQVPYNKLQVIFKSDFSNEERFTGFAAYYVATDINECTDFVDVPCSHFCNNFIGGYFCSCPPEYFLHDDMKNCGVNCSGDVFTALIGEIASPNYPKPYPENSRCEYQIRLEKGFQVVVTLRREDFDVEAADSAGNCLDSLVFVAGDRQFGPYCGHGFPGPLNIETKSNALDIIFQTDLTGQKKGWKLRYHGDPMPCPKEDTPNSVWEPAKAKYVFRDVVQITCLDGFEVVEGRVGATSFYSTCQSNGKWSNSKLKCQPVDCGIPESIENGKVEDPESTLFGSVIRYTCEEPYYYMENGGGGEYHCAGNGSWVNEVLGPELPKCVPVCGVPREPFEEKQRIIGGSDADIKNFPWQVFFDNPWAGGALINEYWVLTAAHVVEGNREPTMYVGSTSVQTSRLAKSKMLTPEHVFIHPGWKLLEVPEGRTNFDNDIALVRLKDPVKMGPTVSPICLPGTSSDYNLMDGDLGLISGWGRTEKRDRAVRLKAARLPVAPLRKCKEVKVEKPTADAEAYVFTPNMICAGGEKGMDSCKGDSGGAFAVQDPNDKTKFYAAGLVSWGPQCGTYGLYTRVKNYVDWIMKTMQENSTPRED 85522647A4C47205 c1s-c1s 2 5 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain human-serum Homo sapiens serum Serum 5 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain human-serum Homo sapiens serum Serum 8 10 direct interaction direct interaction Fig. 6B Interaction is Calcium dependent. c1r-c1s-1 2 3 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain human-serum Homo sapiens serum Serum 5 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain human-serum Homo sapiens serum Serum 15 13 direct interaction direct interaction Fig. 6A, 6C, 6D Interaction is Calcium dependent. c1r-c1s-3 1 5 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain human-serum Homo sapiens serum Serum 3 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain human-serum Homo sapiens serum Serum 4 unspecified role unspecified role neutral component neutral component 18 20 physical association physical association Fig. 7D, 7E 1.87 calcium atoms/mol. The full length proteins bound 4.05 calcium atoms/mol with a Kd = 1.94E-5M. c1s-calcium 1 4 unspecified role unspecified role neutral component neutral component 5 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain human-serum Homo sapiens serum Serum direct interaction direct interaction Fig. 7A, 7B C1S alpha fragment bound 2.96 atom/mol of calcium c1r-c1s-4 1 3 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain human-serum Homo sapiens serum Serum 4 unspecified role unspecified role neutral component neutral component physical association physical association Fig. 7C Repeated equilibrium dialysis experiments performed with native C1R showed that this protein was able to bind Ca*+ in the 10-100uM CaCl2 concentration range, but Scatchard analysis of the binding was not possible, due to the high dispersion of experimental values.