MINT MINT, Dpt of Biology, University of Rome Tor Vergata MINT http://mint.bio.uniroma2.it/mint url>http://mint.bio.uniroma2.it/mint/ https://mint.bio.uniroma2.it MINT-[0-9]+ https://mint.bio.uniroma2.it/index.php/results-interactions/?id=${ac} The Molecular INTeraction database (MINT) is a relational database designed to store interactions between biological molecules. http://mint.bio.uniroma2.it Involvement of PTEN in TPA-mediated p53-activation in mouse skin epidermal JB6 cells. Involvement of PTEN in TPA-mediated p53-activation in mouse skin epidermal JB6 cells. FEBS Lett. (0014-5793) 2012 Robbins D., Ponville J., Morris K., Zhao Y. mouse-jb6 Mus musculus jb6 jb6 fluorescence imaging fluorescence microscopy fluorescence imaging immunostaining immunostaining Robbins D., Ponville J., Morris K., Zhao Y. FEBS Lett. (0014-5793) 2012 Involvement of PTEN in TPA-mediated p53-activation in mouse skin epidermal JB6 cells. Involvement of PTEN in TPA-mediated p53-activation in mouse skin epidermal JB6 cells. FEBS Lett. (0014-5793) 2012 Robbins D., Ponville J., Morris K., Zhao Y. in vitro In vitro solution sedimentati cosedimentation in solution solution sedimentati western blot western blot Immuno blot Immuno blot Robbins D., Ponville J., Morris K., Zhao Y. FEBS Lett. (0014-5793) 2012 Involvement of PTEN in TPA-mediated p53-activation in mouse skin epidermal JB6 cells. Involvement of PTEN in TPA-mediated p53-activation in mouse skin epidermal JB6 cells. FEBS Lett. (0014-5793) 2012 Robbins D., Ponville J., Morris K., Zhao Y. mouse-jb6 Mus musculus jb6 jb6 anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Robbins D., Ponville J., Morris K., Zhao Y. FEBS Lett. (0014-5793) 2012 p53_mouse Cellular tumor antigen p53 Tp53 P53 Trp53 Tumor suppressor p53 protein protein mouse Mus musculus Mouse MTAMEESQSDISLELPLSQETFSGLWKLLPPEDILPSPHCMDDLLLPQDVEEFFEGPSEALRVSGAPAAQDPVTETPGPVAPAPATPWPLSSFVPSQKTYQGNYGFHLGFLQSGTAKSVMCTYSPPLNKLFCQLAKTCPVQLWVSATPPAGSRVRAMAIYKKSQHMTEVVRRCPHHERCSDGDGLAPPQHLIRVEGNLYPEYLEDRQTFRHSVVVPYEPPEAGSEYTTIHYKYMCNSSCMGGMNRRPILTIITLEDSSGNLLGRDSFEVRVCACPGRDRRTEEENFRKKEVLCPELPPGSAKRALPTCTSASPPQKKKPLDGEYFTLKIRGRKRFEMFRELNEALELKDAHATEESGDSRAHSSYLKTKKGQSTSRHKKTMVKKVGPDSD DIP protein P02340 original sequence version: 117 DIP protein P02340 original sequence version: 165 DIP protein P02340 original sequence version: 187 DIP protein P02340 original sequence version: 201 DIP protein P02340 original sequence version: 111 DIP protein P02340 original sequence version: 141 DIP protein P02340 original sequence version: 180 DIP protein P02340 original sequence version: 163 DIP protein P02340 original sequence version: 152 ED5A607378D921D7 pten_mouse Phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN Mutated in multiple advanced cancers 1 Pten Mmac1 Phosphatase and tensin homolog protein protein mouse Mus musculus Mouse MTAIIKEIVSRNKRRYQEDGFDLDLTYIYPNIIAMGFPAERLEGVYRNNIDDVVRFLDSKHKNHYKIYNLCAERHYDTAKFNCRVAQYPFEDHNPPQLELIKPFCEDLDQWLSEDDNHVAAIHCKAGKGRTGVMICAYLLHRGKFLKAQEALDFYGEVRTRDKKGVTIPSQRRYVYYYSYLLKNHLDYRPVALLFHKMMFETIPMFSGGTCNPQFVVCQLKVKIYSSNSGPTRREDKFMYFEFPQPLPVCGDIKVEFFHKQNKMLKKDKMFHFWVNTFFIPGPEETSEKVENGSLCDQEIDSICSIERADNDKEYLVLTLTKNDLDKANKDKANRYFSPNFKVKLYFTKTVEEPSNPEASSSTSVTPDVSDNEPDHYRYSDTTDSDPENEPFDEDQHSQITKV DIP protein O08586 original sequence version: 125 DIP protein O08586 original sequence version: 81 DIP protein O08586 original sequence version: 129 DIP protein O08586 original sequence version: 154 75F97C3DD6843BA9 pten-tp53 1 p53 4 immunostaining immunostaining experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component physiological level physiological level endogenous level endogenous endogenous endogenous level fixed cell fixed cell PTEN 5 immunostaining immunostaining experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component physiological level physiological level endogenous level endogenous endogenous endogenous level fixed cell fixed cell colocalization colocalization f2 mint our data suggest that PTEN and p53 nuclear translocation are redox-regulated in promotion-sensitive P+ cells. pten-tp53-1 3 p53 4 western blot western blot Immuno blot Immuno blot experimental particp experimental participant identification experimental particp unspecified role unspecified role prey prey living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level PTEN 5 western blot western blot Immuno blot Immuno blot experimental particp experimental participant identification experimental particp unspecified role unspecified role bait bait living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level physical association physical association f1b f1c mint We next inquired whether TPA induces an interaction between PTEN and p53 in JB6 P+ and P- cells as a mechanism of p53 activation. We treated P+ and P- cells with TPA for 24 h, analyzed whole cell lysate using immunoprecipitation with anti-PTEN and anti-p53 antibodies; and utilized Western blot analysis to detect protein expression with the corresponding antibodies. As shown in Figure 1B, TPA induced the PTEN-p53 interaction only in JB6 P+ cells. Surprisingly, this interaction was not detected in promotion resistant JB6 P- cells (Fig.1C), suggesting that the PTEN-p53 interaction is correlated with early stage tumor promotion. tp53-pten 3 PTEN 5 western blot western blot Immuno blot Immuno blot experimental particp experimental participant identification experimental particp unspecified role unspecified role prey prey living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level p53 4 western blot western blot Immuno blot Immuno blot experimental particp experimental participant identification experimental particp unspecified role unspecified role bait bait living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level physical association physical association f1b f1c mint We next inquired whether TPA induces an interaction between PTEN and p53 in JB6 P+ and P- cells as a mechanism of p53 activation. We treated P+ and P- cells with TPA for 24 h, analyzed whole cell lysate using immunoprecipitation with anti-PTEN and anti-p53 antibodies; and utilized Western blot analysis to detect protein expression with the corresponding antibodies. As shown in Figure 1B, TPA induced the PTEN-p53 interaction only in JB6 P+ cells. Surprisingly, this interaction was not detected in promotion resistant JB6 P- cells (Fig.1C), suggesting that the PTEN-p53 interaction is correlated with early stage tumor promotion. pten-tp53-2 2 p53 4 western blot western blot Immuno blot Immuno blot experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component subcellular prep subcellular preparation subcellular prep physiological level physiological level endogenous level endogenous endogenous endogenous level PTEN 5 western blot western blot Immuno blot Immuno blot experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component subcellular prep subcellular preparation subcellular prep physiological level physiological level endogenous level endogenous endogenous endogenous level colocalization colocalization f3a mint Western blot analysis revealed that TPA treatment induced increases in both nuclear and cytoplasmic p53; whereas it decreased cytoplasmic but increased nuclear PTEN levels at the 24-h time point in P+ cells (Figure 3A). Previously, we have shown that TPA induces higher levels of ROS in JB6 P+ cells when compared to P- cells [14]. Thus, our data suggest that PTEN and p53 nuclear translocation are redox-regulated in promotion-sensitive P+ cells.