IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Studying protein complexes by the yeast two-hybrid system. Studying protein complexes by the yeast two-hybrid system. Methods (1046-2023) 2012 imex curation Rajagopala S.V., Sikorski P., Harry Caufield J., Tovchigrechko A., Uetz P. rajgsv@gmail.com, peter@uetz.us 18-Dec-2012: Contacted by IntAct-Help. Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast two hybrid array two hybrid array predetermined predetermined participant predetermined Rajagopala S.V., Sikorski P., Harry Caufield J., Tovchigrechko A., Uetz P. rajgsv@gmail.com, peter@uetz.us Accepted 2012-NOV-19 by ORCHARD 18-Dec-2012: Contacted by IntAct-Help. Methods (1046-2023) 2012 Only protein-protein interactions imex curation perr_ecoli HTH-type transcriptional regulator PerR perR Peroxide resistance protein PerR JW0244 b0254 protein protein ecoli Escherichia coli (strain K12) MKLLAKAPLNLLRAFEAAGRTGAFALAASELELSPSAISHAIRKLENLLDVRLFQRSTREITLTKEGEILLEHIQRGFNELQQGLALVTADESRPLRLHTAPSFAHQWLLPRLGKFIRENPSIDLRLSASTEYARFEQDDFDLDIVYGEPRPSPYEKIPLAVEELTPLCSPQLAERLKKPEDLYALTLIQCDVQLYQWKGWFEANKMTPPNNYGLRFDRSFMAIAAAVDGLGVVLESKLLAEREIASGKLVCPLVNSTSEIHYIGHYLVFPQHQHMHSALDVFKTWLLNELNLGKIR DIP protein Q57083 original sequence version: null E197CE2334B7420E dpo3x_ecoli DNA polymerase III subunit tau dnaX dnaZ dnaZX b0470 JW0459 DNA polymerase III subunit gamma protein protein ecoli Escherichia coli (strain K12) MSYQVLARKWRPQTFADVVGQEHVLTALANGLSLGRIHHAYLFSGTRGVGKTSIARLLAKGLNCETGITATPCGVCDNCREIEQGRFVDLIEIDAASRTKVEDTRDLLDNVQYAPARGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEHVKFLLATTDPQKLPVTILSRCLQFHLKALDVEQIRHQLEHILNEEHIAHEPRALQLLARAAEGSLRDALSLTDQAIASGDGQVSTQAVSAMLGTLDDDQALSLVEAMVEANGERVMALINEAAARGIEWEALLVEMLGLLHRIAMVQLSPAALGNDMAAIELRMRELARTIPPTDIQLYYQTLLIGRKELPYAPDRRMGVEMTLLRALAFHPRMPLPEPEVPRQSFAPVAPTAVMTPTQVPPQPQSAPQQAPTVPLPETTSQVLAARQQLQRVQGATKAKKSEPAAATRARPVNNAALERLASVTDRVQARPVPSALEKAPAKKEAYRWKATTPVMQQKEVVATPKALKKALEHEKTPELAAKLAAEAIERDPWAAQVSQLSLPKLVEQVALNAWKEESDNAVCLHLRSSQRHLNNRGAQQKLAEALSMLKGSTVELTIVEDDNPAVRTPLEWRQAIYEEKLAQARESIIADNNIQTLRRFFDAELDEESIRPI DIP protein P06710 original sequence version: 106 DIP protein P06710 original sequence version: null D2028BD99E375150 hold_ecoli DNA polymerase III subunit psi holD b4372 JW4334 protein protein ecoli Escherichia coli (strain K12) MTSRRDWQLQQLGITQWSLRRPGALQGEIAIAIPAHVRLVMVANDLPALTDPLVSDVLRALTVSPDQVLQLTPEKIAMLPQGSHCNSWRLGTDEPLSLEGAQVASPALTDLRANPTARAALWQQICTYEHDFFPRND DIP protein P28632 original sequence version: 88 DIP protein P28632 original sequence version: null 5B7356201E7E27FE mntr_ecoli Transcriptional regulator MntR ybiQ Manganese transport regulator mntR b0817 JW0801 protein protein ecoli Escherichia coli (strain K12) MSRRAGTPTAKKVTQLVNVEEHVEGFRQVREAHRRELIDDYVELISDLIREVGEARQVDMAARLGVSQPTVAKMLKRLATMGLIEMIPWRGVFLTAEGEKLAQESRERHQIVENFLLVLGVSPEIARRDAEGMEHHVSEETLDAFRLFTQKHGAK DIP protein P0A9F1 original sequence version: null CB316AC7F1BDD106 yjhc_ecoli Uncharacterized oxidoreductase YjhC yjhC b4280 JW5769 protein protein ecoli Escherichia coli (strain K12) MINYGVVGVGYFGAELARFMNMHDNAKITCVYDPENGENIARELQCINMSSLDALVSSKLVDCVIVATPNYLHKEPVIKAAKNKKHVFCEKPIALSYEDCVDMVKACKEAGVTFMAGHIMNFFNGVQYARKLIKEGVIGEILSCHTKRNGWENKQERLSWKKMKEQSGGHLYHHIHELDCVQHLLGEIPETVTMIGGNLAHSGPGFGNEDDMLFMTLEFPSGKLATLEWGSAFNWPEHYVIINGTKGSIKIDMQETAGSLRIGGQTKHFLVHETQEEDDDRRKGNMTSEMDGAIAYGHPGKKTPLWLASLIRKETLFLHNILCGAKPEEDYIDLLNGEAAMSAIATADAATLSRSQDRKVKISEIIKHTSVM DIP protein P39353 original sequence version: null 34B2E535EADC2ABE yhjb_ecoli Putative HTH-type transcriptional regulator YhjB yhjB b3520 JW3488 protein protein ecoli Escherichia coli (strain K12) MQIVMFDRQSIFIHGMKISLQQRIPGVSIQGASQADELWQKLESYPEALVMLDGDQDGEFCYWLLQKTVVQFPEVKVLITATDCNKRWLQEVIHFNVLAIVPRDSTVETFALAVNSAAMGMMFLPGDWRTTPEKDIKDLKSLSARQREILTMLAAGESNKEIGRALNISTGTVKAHLESLYRRLEVKNRTQAAMMLNISS DIP protein P37640 original sequence version: null D238AB59FE6ED109 hycg_ecoli Formate hydrogenlyase subunit 7 Hydrogenase-3 component G hycG hevG b2719 JW2689 protein protein ecoli Escherichia coli (strain K12) MSNLLGPRDANGIPVPMTVDESIASMKASLLKKIKRSAYVYRVDCGGCNGCEIEIFATLSPLFDAERFGIKVVPSPRHADILLFTGAVTRAMRSPALRAWQSAPDPKICISYGACGNSGGIFHDLYCVWGGTDKIVPVDVYIPGCPPTPAATLYGFAMALGLLEQKIHARGPGELDEQPAEILHGDMVQPLRVKVDREARRLAGYRYGRQIADDYLTQLGQGEEQVARWLEAENDPRLNEIVSHLNHVVEEARIR DIP protein P16433 original sequence version: null B24FBAE3C44F3A10 asnb_ecoli Asparagine synthetase B [glutamine-hydrolyzing] asnB b0674 JW0660 protein protein ecoli Escherichia coli (strain K12) MCSIFGVFDIKTDAVELRKKALELSRLMRHRGPDWSGIYASDNAILAHERLSIVDVNAGAQPLYNQQKTHVLAVNGEIYNHQALRAEYGDRYQFQTGSDCEVILALYQEKGPEFLDDLQGMFAFALYDSEKDAYLIGRDHLGIIPLYMGYDEHGQLYVASEMKALVPVCRTIKEFPAGSYLWSQDGEIRSYYHRDWFDYDAVKDNVTDKNELRQALEDSVKSHLMSDVPYGVLLSGGLDSSIISAITKKYAARRVEDQERSEAWWPQLHSFAVGLPGSPDLKAAQEVANHLGTVHHEIHFTVQEGLDAIRDVIYHIETYDVTTIRASTPMYLMSRKIKAMGIKMVLSGEGSDEVFGGYLYFHKAPNAKELHEETVRKLLALHMYDCARANKAMSAWGVEARVPFLDKKFLDVAMRINPQDKMCGNGKMEKHILRECFEAYLPASVAWRQKEQFSDGVGYSWIDTLKEVAAQQVSDQQLETARFRFPYNTPTSKEAYLYREIFEELFPLPSAAECVPGGPSVACSSAKAIEWDEAFKKMDDPSGRAVGVHQSAYK DIP protein P22106 original sequence version: null 9091B269112C9F5C dpo3a_ecoli DNA polymerase III subunit alpha dnaE polC b0184 JW0179 protein protein ecoli Escherichia coli (strain K12) MSEPRFVHLRVHSDYSMIDGLAKTAPLVKKAAALGMPALAITDFTNLCGLVKFYGAGHGAGIKPIVGADFNVQCDLLGDELTHLTVLAANNTGYQNLTLLISKAYQRGYGAAGPIIDRDWLIELNEGLILLSGGRMGDVGRSLLRGNSALVDECVAFYEEHFPDRYFLELIRTGRPDEESYLHAAVELAEARGLPVVATNDVRFIDSSDFDAHEIRVAIHDGFTLDDPKRPRNYSPQQYMRSEEEMCELFADIPEALANTVEIAKRCNVTVRLGEYFLPQFPTGDMSTEDYLVKRAKEGLEERLAFLFPDEEERLKRRPEYDERLETELQVINQMGFPGYFLIVMEFIQWSKDNGVPVGPGRGSGAGSLVAYALKITDLDPLEFDLLFERFLNPERVSMPDFDVDFCMEKRDQVIEHVADMYGRDAVSQIITFGTMAAKAVIRDVGRVLGHPYGFVDRISKLIPPDPGMTLAKAFEAEPQLPEIYEADEEVKALIDMARKLEGVTRNAGKHAGGVVIAPTKITDFAPLYCDEEGKHPVTQFDKSDVEYAGLVKFDFLGLRTLTIINWALEMINKRRAKNGEPPLDIAAIPLDDKKSFDMLQRSETTAVFQLESRGMKDLIKRLQPDCFEDMIALVALFRPGPLQSGMVDNFIDRKHGREEISYPDVQWQHESLKPVLEPTYGIILYQEQVMQIAQVLSGYTLGGADMLRRAMGKKKPEEMAKQRSVFAEGAEKNGINAELAMKIFDLVEKFAGYGFNKSHSAAYALVSYQTLWLKAHYPAEFMAAVMTADMDNTEKVVGLVDECWRMGLKILPPDINSGLYHFHVNDDGEIVYGIGAIKGVGEGPIEAIIEARNKGGYFRELFDLCARTDTKKLNRRVLEKLIMSGAFDRLGPHRAALMNSLGDALKAADQHAKAEAIGQADMFGVLAEEPEQIEQSYASCQPWPEQVVLDGERETLGLYLTGHPINQYLKEIERYVGGVRLKDMHPTERGKVITAAGLVVAARVMVTKRGNRIGICTLDDRSGRLEVMLFTDALDKYQQLLEKDRILIVSGQVSFDDFSGGLKMTAREVMDIDEAREKYARGLAISLTDRQIDDQLLNRLRQSLEPHRSGTIPVHLYYQRADARARLRFGATWRVSPSDRLLNDLRGLIGSEQVELEFD DIP protein P10443 original sequence version: 103 DIP protein P10443 original sequence version: null 1A4F75F373841716 trmd_ecoli tRNA (guanine-N(1)-)-methyltransferase trmD b2607 JW2588 M1G-methyltransferase tRNA [GM37] methyltransferase protein protein ecoli Escherichia coli (strain K12) MWIGIISLFPEMFRAITDYGVTGRAVKNGLLSIQSWSPRDFTHDRHRTVDDRPYGGGPGMLMMVQPLRDAIHAAKAAAGEGAKVIYLSPQGRKLDQAGVSELATNQKLILVCGRYEGIDERVIQTEIDEEWSIGDYVLSGGELPAMTLIDSVSRFIPGVLGHEASATEDSFAEGLLDCPHYTRPEVLEGMEVPPVLLSGNHAEIRRWRLKQSLGRTWLRRPELLENLALTEEQARLLAEFKTEHAQQQHKHDGMA B101087229B4CDBD metn_ecoli Methionine import ATP-binding protein MetN metN abc b0199 JW0195 protein protein ecoli Escherichia coli (strain K12) MIKLSNITKVFHQGTRTIQALNNVSLHVPAGQIYGVIGASGAGKSTLIRCVNLLERPTEGSVLVDGQELTTLSESELTKARRQIGMIFQHFNLLSSRTVFGNVALPLELDNTPKDEVKRRVTELLSLVGLGDKHDSYPSNLSGGQKQRVAIARALASNPKVLLCDEATSALDPATTRSILELLKDINRRLGLTILLITHEMDVVKRICDCVAVISNGELIEQDTVSEVFSHPKTPLAQKFIQSTLHLDIPEDYQERLQAEPFTDCVPMLRLEFTGQSVDAPLLSETARRFNVNNNIISAQMDYAGGVKFGIMLTEMHGTQQDTQAAIAWLQEHHVKVEVLGYV DIP protein P30750 original sequence version: 94 DIP protein P30750 original sequence version: null 2FC796C605216FB3 holc_ecoli DNA polymerase III subunit chi holC b4259 JW4216 protein protein ecoli Escherichia coli (strain K12) MKNATFYLLDNDTTVDGLSAVEQLVCEIAAERWRSGKRVLIACEDEKQAYRLDEALWARPAESFVPHNLAGEGPRGGAPVEIAWPQKRSSSRRDILISLRTSFADFATAFTEVVDFVPYEDSLKQLARERYKAYRVAGFNLNTATWK 7FEFFC359B28AA88 7FEFFC359B28AA88 dnae-dnax 1 10 unspecified role unspecified role bait bait 3 unspecified role unspecified role prey prey physical association physical association Fig. 1b, c hold-dnax 1 4 unspecified role unspecified role bait bait 3 unspecified role unspecified role prey prey physical association physical association Fig. 1b, c hold-holc 1 4 unspecified role unspecified role bait bait 13 unspecified role unspecified role prey prey physical association physical association Fig. 1b, c holc-hold 1 4 unspecified role unspecified role prey prey 13 unspecified role unspecified role bait bait physical association physical association Fig. 1b, c asnb-asnb 1 9 unspecified role unspecified role bait bait 9 unspecified role unspecified role prey prey physical association physical association Fig. 1b, c trmd-trmd 1 11 unspecified role unspecified role prey prey 11 unspecified role unspecified role bait bait physical association physical association Fig. 1b, c hold-hold 1 4 unspecified role unspecified role prey prey 4 unspecified role unspecified role bait bait physical association physical association Fig. 1b, c perr-perr 1 2 unspecified role unspecified role prey prey 2 unspecified role unspecified role bait bait physical association physical association Fig.1d mntr-yhjb 1 5 unspecified role unspecified role unspecified role unspecified role 7 unspecified role unspecified role unspecified role unspecified role physical association physical association Fig.1d mntr-yjhc 1 6 unspecified role unspecified role unspecified role unspecified role 5 unspecified role unspecified role unspecified role unspecified role physical association physical association Fig.1d hycg-hycg 1 8 unspecified role unspecified role prey prey 8 unspecified role unspecified role bait bait physical association physical association Fig.1d mntr-hycg 1 8 unspecified role unspecified role unspecified role unspecified role 5 unspecified role unspecified role unspecified role unspecified role physical association physical association Fig.1d yjhc-yjhc 1 6 unspecified role unspecified role prey prey 6 unspecified role unspecified role bait bait physical association physical association Fig.1d perr-mntr 1 2 unspecified role unspecified role unspecified role unspecified role 5 unspecified role unspecified role unspecified role unspecified role physical association physical association Fig.1d mntr-mntr 1 5 unspecified role unspecified role prey prey 5 unspecified role unspecified role bait bait physical association physical association Fig.1d hycg-metn 1 12 unspecified role unspecified role unspecified role unspecified role 8 unspecified role unspecified role unspecified role unspecified role physical association physical association Fig.1d metn-mntr 1 5 unspecified role unspecified role unspecified role unspecified role 12 unspecified role unspecified role unspecified role unspecified role physical association physical association Fig. 1D metn-yjhc 1 12 unspecified role unspecified role unspecified role unspecified role 6 unspecified role unspecified role unspecified role unspecified role physical association physical association Fig. 1D