IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ A Translation-Like Cycle Is a Quality Control Checkpoint for Maturing 40S Ribosome Subunits. A Translation-Like Cycle Is a Quality Control Checkpoint for Maturing 40S Ribosome Subunits. Cell (0092-8674) 2012 imex curation Strunk B.S., Novak M.N., Young C.L., Karbstein K. Sucrose gradients are run from whole cell lysates so I have not included this data Formaldehyde crosslinking followed by TAP purification kkarbst@scripps.edu 19-Sept-2012: Contacted by IntAct-Help. Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast crosslink cross-linking study crosslink sequence tag sequence tag identification sequence tag Strunk B.S., Novak M.N., Young C.L., Karbstein K. Sucrose gradients are run from whole cell lysates so I have not included this data Formaldehyde crosslinking followed by TAP purification kkarbst@scripps.edu Accepted 2012-AUG-01 by PPORRAS 19-Sept-2012: Contacted by IntAct-Help. Cell (0092-8674) 2012 Only protein-protein interactions imex curation ef1b_yeast Elongation factor 1-beta EFB1 TEF5 Translation elongation factor 1B alpha Eukaryotic elongation factor 1Balpha YAL003W protein protein yeast Saccharomyces cerevisiae Baker's yeast MASTDFSKIETLKQLNASLADKSYIEGTAVSQADVTVFKAFQSAYPEFSRWFNHIASKADEFDSFPAASAAAAEEEEDDDVDLFGSDDEEADAEAEKLKAERIAAYNAKKAAKPAKPAAKSIVTLDVKPWDDETNLEEMVANVKAIEMEGLTWGAHQFIPIGFGIKKLQINCVVEDDKVSLDDLQQSIEEDEDHVQSTDIAAMQKL BEA764852CE125F7 BEA764852CE125F7 dim1_yeast Dimethyladenosine transferase YPL266W DIM1 S-adenosylmethionine-6-N', N'-adenosyl(rRNA) dimethyltransferase 18S rRNA dimethylase 18S rRNA (adenine(1779)-N(6)/adenine(1780)-N(6))-dimethyltransferase protein protein yeast Saccharomyces cerevisiae Baker's yeast MGKAAKKKYSGATSSKQVSAEKHLSSVFKFNTDLGQHILKNPLVAQGIVDKAQIRPSDVVLEVGPGTGNLTVRILEQAKNVVAVEMDPRMAAELTKRVRGTPVEKKLEIMLGDFMKTELPYFDICISNTPYQISSPLVFKLINQPRPPRVSILMFQREFALRLLARPGDSLYCRLSANVQMWANVTHIMKVGKNNFRPPPQVESSVVRLEIKNPRPQVDYNEWDGLLRIVFVRKNRTISAGFKSTTVMDILEKNYKTFLAMNNEMVDDTKGSMHDVVKEKIDTVLKETDLGDKRAGKCDQNDFLRLLYAFHQVGIHFS EE77C9DF574A042A EE77C9DF574A042A rla0_yeast 60S acidic ribosomal protein P0 RPP0 L10E RPA0 RPL10E RPLA0 YLR340W L8300.8 L10e Large ribosomal subunit protein uL10 protein protein yeast Saccharomyces cerevisiae Baker's yeast MGGIREKKAEYFAKLREYLEEYKSLFVVGVDNVSSQQMHEVRKELRGRAVVLMGKNTMVRRAIRGFLSDLPDFEKLLPFVKGNVGFVFTNEPLTEIKNVIVSNRVAAPARAGAVAPEDIWVRAVNTGMEPGKTSFFQALGVPTKIARGTIEIVSDVKVVDAGNKVGQSEASLLNLLNISPFTFGLTVVQVYDNGQVFPSSILDITDEELVSHFVSAVSTIASISLAIGYPTLPSVGHTLINNYKDLLAVAIAASYHYPEIEDLVDRIENPEKYAAAAPAATSAASGDAAPAEEAAAEEEEESDDDMGFGLFD 109ECC90F7C8B68E 109ECC90F7C8B68E rs12_yeast 40S ribosomal protein S12 RPS12 Small ribosomal subunit protein eS12 YOR369C protein protein yeast Saccharomyces cerevisiae Baker's yeast MSDVEEVVEVQEETVVEQTAEVTIEDALKVVLRTALVHDGLARGLRESTKALTRGEALLVVLVSSVTEANIIKLVEGLANDPENKVPLIKVADAKQLGEWAGLGKIDREGNARKVVGASVVVVKNWGAETDELSMIMEHFSQQ 7BD0434EB06A26B3 kad6_yeast Adenylate kinase isoenzyme 6 homolog FAP7 FAP7 POS9-activating factor 7 YDL166C Dual activity adenylate kinase/ATPase protein protein yeast Saccharomyces cerevisiae Baker's yeast MEARRYGPNIIVTGTPGCGKSSTCEFLKNKLKDYKYYNISDFAKDNDCFEGYDEGRKSHIVDEDKLLDMLEPLLRQGNSIVDWHVNDVFPERLIDLVVVLRCDNSNLYSRLHARGYHDSKIEENLDAEIMGVVKQDAVESYEPHIVVELQSDTKEDMVSNVSRIVAWEKMWLEQHPDGVTNEYQGPRSDDEDDEDSE 836A25B0D7E2633C 836A25B0D7E2633C vate_yeast V-type proton ATPase subunit E VMA4 VAT5 O6241 YOR332W Vacuolar proton pump subunit E V-ATPase 27 kDa subunit protein protein yeast Saccharomyces cerevisiae Baker's yeast MSSAITALTPNQVNDELNKMQAFIRKEAEEKAKEIQLKADQEYEIEKTNIVRNETNNIDGNFKSKLKKAMLSQQITKSTIANKMRLKVLSAREQSLDGIFEETKEKLSGIANNRDEYKPILQSLIVEALLKLLEPKAIVKALERDVDLIESMKDDIMREYGEKAQRAPLEEIVISNDYLNKDLVSGGVVVSNASDKIEINNTLEERLKLLSEEALPAIRLELYGPSKTRKFFD 7AC5169FF7E5A39C 7AC5169FF7E5A39C gblp_yeast Guanine nucleotide-binding protein subunit beta-like protein ASC1 CPC2 Receptor of activated protein kinase C 1 Receptor for activated C kinase Small ribosomal subunit protein RACK1 YM9718.15C YMR116C protein protein yeast Saccharomyces cerevisiae Baker's yeast MASNEVLVLRGTLEGHNGWVTSLATSAGQPNLLLSASRDKTLISWKLTGDDQKFGVPVRSFKGHSHIVQDCTLTADGAYALSASWDKTLRLWDVATGETYQRFVGHKSDVMSVDIDKKASMIISGSRDKTIKVWTIKGQCLATLLGHNDWVSQVRVVPNEKADDDSVTIISAGNDKMVKAWNLNQFQIEADFIGHNSNINTLTASPDGTLIASAGKDGEIMLWNLAAKKAMYTLSAQDEVFSLAFSPNRYWLAAATATGIKVFSLDPQYLVDDLRPEFAGYSKAAEPHAVSLAWSADGQTLFAGYTDNVIRVWQVMTAN B9A5D4959A3C54FB B9A5D4959A3C54FB tsa1_yeast Peroxiredoxin TSA1 TSA1 TPX1 TSA YML028W Cytoplasmic thiol peroxidase 1 Thiol-specific antioxidant protein 1 ZRG14 Protector protein Thioredoxin peroxidase type Ia Thioredoxin-dependent peroxide reductase Thioredoxin-dependent peroxiredoxin TSA1 protein protein yeast Saccharomyces cerevisiae Baker's yeast MVAQVQKQAPTFKKTAVVDGVFDEVSLDKYKGKYVVLAFIPLAFTFVCPTEIIAFSEAAKKFEEQGAQVLFASTDSEYSLLAWTNIPRKEGGLGPINIPLLADTNHSLSRDYGVLIEEEGVALRGLFIIDPKGVIRHITINDLPVGRNVDEALRLVEAFQWTDKNGTVLPCNWTPGAATIKPTVEDSKEYFEAANK 9ACF40E410F2C04A 9ACF40E410F2C04A ola1_yeast Obg-like ATPase 1 YBR025C YBR0309 OLA1 protein protein yeast Saccharomyces cerevisiae Baker's yeast MPPKKQVEEKKVLLGRPGNNLKAGIVGLANVGKSTFFQAITRCPLGNPANYPFATIDPEEARVIVPSPRFDKLCEIYKKTASEVPAHLTVYDIAGLTKGASAGEGLGNAFLSHIRSVDSIYQVVRCFDDAEIIHVEGDVDPVRDLEIINQELRLKDIEFAQKALEGAEKIAKRGGQSLEVKQKKEEMDLITKIIKLLESGQRVANHSWTSKEVEIINSMFLLTAKPCIYLINLSERDYIRKKNKHLLRIKEWVDKYSPGDLIIPFSVSLEERLSHMSPEDAEEELKKLQTISALPKIITTMRQKLDLISFFTCGPDEVREWTIRRGTKAPQAAGVIHNDLMNTFILAQVMKCEDVFEYKDDSAIKAAGKLMQKGKDYVVEDGDIIYFRAGAGKN 4D3E59931A155BCB 4D3E59931A155BCB ef1a_yeast Elongation factor 1-alpha TEF1 YPR080W P9513.7 TEF2 YBR118W YBR0913 Translation elongation factor 1A Eukaryotic elongation factor 1A protein protein yeast Saccharomyces cerevisiae Baker's yeast MGKEKSHINVVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAAELGKGSFKYAWVLDKLKAERERGITIDIALWKFETPKYQVTVIDAPGHRDFIKNMITGTSQADCAILIIAGGVGEFEAGISKDGQTREHALLAFTLGVRQLIVAVNKMDSVKWDESRFQEIVKETSNFIKKVGYNPKTVPFVPISGWNGDNMIEATTNAPWYKGWEKETKAGVVKGKTLLEAIDAIEQPSRPTDKPLRLPLQDVYKIGGIGTVPVGRVETGVIKPGMVVTFAPAGVTTEVKSVEMHHEQLEQGVPGDNVGFNVKNVSVKEIRRGNVCGDAKNDPPKGCASFNATVIVLNHPGQISAGYSPVLDCHTAHIACRFDELLEKNDRRSGKKLEDHPKFLKSGDAALVKFVPSKPMCVEAFSEYPPLGRFAVRDMRQTVAVGVIKSVDKTEKAAKVTKAAQKAAKK 411C66D830716576 411C66D830716576 metk1_yeast S-adenosylmethionine synthase 1 SAM1 ETH10 YLR180W L9470.9 Methionine adenosyltransferase 1 protein protein yeast Saccharomyces cerevisiae Baker's yeast MAGTFLFTSESVGEGHPDKICDQVSDAILDACLAEDPHSKVACETAAKTGMIMVFGEITTKAQLDYQKIVRDTIKKIGYDDSAKGFDYKTCNVLVAIEQQSPDIAQGVHEEKDLEDIGAGDQGIMFGYATDETPEGLPLTILLAHKLNMAMADARRDGSLAWLRPDTKTQVTVEYKDDHGRWVPQRIDTVVVSAQHADEITTEDLRAQLKSEIIEKVIPRDMLDENTKYFIQPSGRFVIGGPQGDAGLTGRKIIVDAYGGASSVGGGAFSGKDYSKVDRSAAYAARWVAKSLVAAGLCKRVQVQFSYAIGIAEPLSLHVDTYGTATKSDEEIIDIISKNFDLRPGVLVKELDLARPIYLPTASYGHFTNQEYPWEKPKTLKF 18F82FC809EE4706 18F82FC809EE4706 bmh2_yeast Protein BMH2 YD8557.08 YDR099W BMH2 protein protein yeast Saccharomyces cerevisiae Baker's yeast MSQTREDSVYLAKLAEQAERYEEMVENMKAVASSGQELSVEERNLLSVAYKNVIGARRASWRIVSSIEQKEESKEKSEHQVELIRSYRSKIETELTKISDDILSVLDSHLIPSATTGESKVFYYKMKGDYHRYLAEFSSGDAREKATNSSLEAYKTASEIATTELPPTHPIRLGLALNFSVFYYEIQNSPDKACHLAKQAFDDAIAELDTLSEESYKDSTLIMQLLRDNLTLWTSDISESGQEDQQQQQQQQQQQQQQQQQAPAEQTQGEPTK 74788A9103F129FB 74788A9103F129FB if4a_yeast ATP-dependent RNA helicase eIF4A Stimulator factor I 37 kDa component TIF41A TIF41B Translation initiation factor 1/2 J0660 YJL138C YKR059W TIF1 TIF2 Eukaryotic initiation factor 4A p37 protein protein yeast Saccharomyces cerevisiae Baker's yeast MSEGITDIEESQIQTNYDKVVYKFDDMELDENLLRGVFGYGFEEPSAIQQRAIMPIIEGHDVLAQAQSGTGKTGTFSIAALQRIDTSVKAPQALMLAPTRELALQIQKVVMALAFHMDIKVHACIGGTSFVEDAEGLRDAQIVVGTPGRVFDNIQRRRFRTDKIKMFILDEADEMLSSGFKEQIYQIFTLLPPTTQVVLLSATMPNDVLEVTTKFMRNPVRILVKKDELTLEGIKQFYVNVEEEEYKYECLTDLYDSISVTQAVIFCNTRRKVEELTTKLRNDKFTVSAIYSDLPQQERDTIMKEFRSGSSRILISTDLLARGIDVQQVSLVINYDLPANKENYIHRIGRGGRFGRKGVAINFVTNEDVGAMRELEKFYSTQIEELPSDIATLLN 19D8C133C815DD48 19D8C133C815DD48 rl5_yeast 60S ribosomal protein L5 RPL1 RPL1A RPL5 YPL131W LPI14W L1 L1a YL3 Ribosomal 5S RNA-binding protein Large ribosomal subunit protein uL18 protein protein yeast Saccharomyces cerevisiae Baker's yeast MAFQKDAKSSAYSSRFQTPFRRRREGKTDYYQRKRLVTQHKAKYNTPKYRLVVRFTNKDIICQIISSTITGDVVLAAAYSHELPRYGITHGLTNWAAAYATGLLIARRTLQKLGLDETYKGVEEVEGEYELTEAVEDGPRPFKVFLDIGLQRTTTGARVFGALKGASDGGLYVPHSENRFPGWDFETEEIDPELLRSYIFGGHVSQYMEELADDDEERFSELFKGYLADDIDADSLEDIYTSAHEAIRADPAFKPTEKKFTKEQYAAESKKYRQTKLSKEERAARVAAKIAALAGQQ 14E3B2931C3CAE2B 14E3B2931C3CAE2B fap7-asc1 1 5 unspecified role unspecified role prey prey 9 unspecified role unspecified role prey prey 13 unspecified role unspecified role prey prey 2 unspecified role unspecified role prey prey 10 unspecified role unspecified role prey prey 3 unspecified role unspecified role prey prey 7 unspecified role unspecified role prey prey 14 unspecified role unspecified role prey prey 11 unspecified role unspecified role prey prey 15 unspecified role unspecified role prey prey 4 unspecified role unspecified role prey prey 6 unspecified role unspecified role bait bait region tap tagged calmodulin binding peptide plus protein a tag CBP-ProtA tagged CBP-ProtA tagged tap tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 12 unspecified role unspecified role prey prey 8 unspecified role unspecified role prey prey association association Table 1 RPS0, RPS7, RPS14, RPS16, RPS18, RPS19, RPS22, RPS24, RPL6, RPL7, RPL11, RPL12, RPL14, RPL23, RPL26, eIF5A were also identified but could not be ascribed to a single gene product.