mpidb The Microbial Protein Interaction Database LapG, required for modulating biofilm formation by Pseudomonas fluorescens Pf0-1, is a calcium-dependent protease. LapG, required for modulating biofilm formation by Pseudomonas fluorescens Pf0-1, is a calcium-dependent protease. J. Bacteriol. (0021-9193) 2012 imex curation Boyd C.D., Chatterjee D., Sondermann H., O'Toole G.A. 18-Dec-2012: Contacted by IntAct-Help. Only protein-protein interactions psefl Pseudomonas fluorescens pull down pull down anti tag western anti tag western blot anti tag western Boyd C.D., Chatterjee D., Sondermann H., O'Toole G.A. Accepted 2012-DEC-05 by ORCHARD 18-Dec-2012: Contacted by IntAct-Help. J. Bacteriol. (0021-9193) 2012 Only protein-protein interactions imex curation LapG, required for modulating biofilm formation by Pseudomonas fluorescens Pf0-1, is a calcium-dependent protease. LapG, required for modulating biofilm formation by Pseudomonas fluorescens Pf0-1, is a calcium-dependent protease. J. Bacteriol. (0021-9193) 2012 imex curation Boyd C.D., Chatterjee D., Sondermann H., O'Toole G.A. 18-Dec-2012: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro protease assay protease assay predetermined predetermined participant predetermined Boyd C.D., Chatterjee D., Sondermann H., O'Toole G.A. Accepted 2012-DEC-05 by ORCHARD 18-Dec-2012: Contacted by IntAct-Help. J. Bacteriol. (0021-9193) 2012 Only protein-protein interactions imex curation q3kk29_psepf lapA Pfl01_0133 protein protein psepf Pseudomonas fluorescens MTPGESSMSSVVAIVKSIVGQVFVVSPEGVRRVLVEGDRLFAGDQVDTGVSGAVSLELADGRTIDLGRDTQWSADTPDSSTDLAEATAQAAPSVEELQQAIAAGVDPTTALESTAAGPSAAGTGGAAGGGHSFVMLDATAGRVDPTIGFPTAGINAAGQAAQNITGGQNTDTTANALRESTLSLSATPSITEAGGVLVYTATLTQAPLTDLTITLSNGAVIVIPAGSTTGTANVPLAPNDTVYNDPTQIDVTVTGTTGGSGITVTPPTTPATTQVTDTIDTTTVTLTAGPSVTEGGQITYTATLTNPAQTPVTVTLSNGSTITIGAGQTTGTVNVPTAPNDVYNNGSTVTTTITGATGGNFENLVPNPTPAVTTITDSIDTTGLTLTATGTVVEGGQITYTATLTNPAQTPVTVTLSNGSTITIGAGQTTGSVNVPTAPNDVYNNGGTVSTTITGATGGNFENLVPNTTPATTTVTDSIDTTNLSLSATGSVAEGGSIVYTATLTNPAGTPVTVTLSNGSVITIEAGKTTGTVTVPAPADDVYKDAGKIEVTIKDATGGNFENLVPSTVPAVTEVTDTIDTSTVKLTADSSVAEGGTVTYTATVGAPVTGSPVTVTLANGQTITIEVGKTTGTVTTTAPNDALAGNAPLTNSITGVSGGNYENLVADKTPVSTTVTDVADITNLTLSATGTVAEGGSIVYTATLTNPAGTPVTVTLSNGSVITIEAGKTTGTVTVPAPADDVYKDAGKVEVTIKDASGGNFENLVPSTVPAVTEVTDTIDTSTVKLSADTSVAEGGTVTYTATVGAPVTGSPVTVTLANGQTITIEVGKTTGTITTTAPNDVLTGHAPLTNSITGVSGGNYENLVADKTPVSTTVTDVADTTNLTLSATGTVAEGGSIVYTATLTNAAGTPVTVTLSNGAVITIEAGKTTGTVTVPAPADDVYKDAGKVEVTIKDATGGNFENLVPSTVPAVTNVTDTIDTTTVKLTATESAVEGGTVTYTATVGAPVTGSPVTVTLANGQSITIEVGKTTGTITTTAPNDALNGHTPLTNAITGVSGGNYENLVADKTPVSTTVTDTVDTTNLTLSASDSVAEGGSIVYTATLTNPAGTSVTVTLSNGAVITIEAGKTTGTVTVPAPADDVYKDAGKVEVTIKDASGGNFENLVPSTVPAVTEVTDTIDTTTVKLTATESAAEGGTVTYTATVGAPVTGSPVTVTLANGQSITIEVGKTTGTVTTTAPNDALNGHTPLTNAITGVSGGNYENLVADKTPVSTRVTDTVDTTNLTLSATGTVAEGGSIVYTATLTNPAGTPVTVTLSNGSVITIEAGKTTGTVTVPAPADDVYKDAGKVEVTIKDATGGNFENLVPSTVPAVTNVTDTIDTSTVKLSADTSVAEGGTVTYTATVGAPVTGSPVTVTLANGQTITIEVGKTTGTITTTAPNDALNGHTPLSNSITGVTGGNYENLVADKTPVSTTVTDTVDTTDLTLTATDSVAEGGSITYTATLTNAAGTPVTVTLSNGAVITIEAGKTTGTVTVDAPKDDVYKDAGKVEVTIKDATGGNFENLVPSTVPAVTEVTDTIDTTTVKLTATESAAEGGTVTYTATVGAPVTGSPVTVTLANGQSITIEVGKTTGTITTTAPNDALNGHTPLTNAITGVSGGNYENLVADKTPVSTTVTDTVDTTNLTLSASDSVAEGGSIVYTATLTNPAGTPVTVTLSNGAVITIEAGKTTGTVTVDAPKDDVYKDAGKVEATISTATGGNFENLVPSTVPAVTEVTDTIDTTTVKLTATESAVEGGTVSYTATVGAPVTGSPVTVTLANGQSITIEVGKTTGTVTTTAPNDVLNGHAPLTNAITNVTGGNYENLVADKTPVSTTVTDTVDTTNLTLSATDSVAEGGSIVYTATLTNPAGTPVTVTLSNGAVITIEAGKTTGTVTVDAPKDDVYKDAGKVEVTIKDATGGNFENLVPSTAPAVTEVTDTIDTSTVKLTATESAAEGGTVTYTATVGAPVTGSPVTVTLANGQSITIEVGKTTGTVTTTAPNDALNGHTPLTNSITDVSGGNYENLVADKTPVSTTVTDTVDTTNLTLSATPEVNEGGQITYTATLTNAAGTPVTVTLSNGAVITIEAGKTTGTVTVDAPKDDVYKDAGKVETTISTTTGGNFENLVTSTAPAVTTVNDTIDTSTVSLTATANVAEGETVVYTATVTAPVTGSPVVVTLSNGQTITIAVGETTGTVNFVAPNSPLAGGSSLSVTIDKATGGNYENLAVDGKSADTSVSDTVDTTNLNLTATDSVAEGGSITYTATLTNPAGTPVTVTLSNGAVITIEAGKTTGTVTVDAPKDDVYKDAGKVEATISTATGGGFENLVPSTVPAVTNVTDTIDTTTVKLTATESAAEGGTVTYTATVGAPVTGSPVTVTLANGQSITIEVGKTTGTVTTTAPNDALNGHTPLTNSITGVSGGNYENLVADKTPVSTTVTDTVDTTNLTLSATGSVAEGGSIVYTATLTNPAGTPVTVTLSNGAVITIEAGKTIGTVTVPAPADDVYKDAGKVEVTIKDATGGNFENLVPSTVPAVTEVTDTIDTTTVKLTASETAAEGGTVTYTATVGAPVTGSPVTVTLANGQQITIEVGKTTGTVTTTAPNDVLNDHAPLTNAITDVSGGNYENLVADKTPVSTTITDTIDTTNLSLTATGTVAEGGSIVYTATLTNPAGTPVTVTLSNGSVITIEAGKTTGTVTVPAPADDVYKDAGTVEATIKSATGGNFENLVTSTTPAVTNVTDTIDTTTVKLTATESAAEGGTVTYTATVGAPVTGSPVTVTLSNGQSITIEVGKTTGTVTTTAPNDALTGHAPLTNAITGVTGGNYENLVADKTPVSTTVTDTVDTTNLTLSATNSVAEGGSIIYTATLTNAAGSPVTVTLSNGAVITIEAGKTTGTVTVPAPADDVYKDAGNVQATITNATGGNFENLVPSTVPAVTQVTDTIDTTTVKLTATESAAEGGTVTYTATVGAPVTGSPVTVNLSNGQSITIEVGKTTGTVTTTAPNDVLNGHAPLTNAITGVTGGNYENLVADKTPVSTTVTDTVDTTNLSLSATGTVAEGGSITYTATLTNAAGTPVTVTLSNGAVIIIEAGKTTGTVTVAAPADDVYKDAGNVQATITNATGGNFENLVTSTTPAVTSVTDTIDTTTVKLTATATAAEGGTVTYTATVGAPVTGSPVTVNLSNGQSITIEVGKTTGTVTTTAPNDVLTGHAPLTNAITGVSGGNYENLVADKTPVSTTVTDTVDTTNLSLSATGTVAEGGSITYTATLTNAAGSPVTVTLSNGAVITIEAGKTTGTVTVPAPADDVYKDAGNVQATITNATGGNFENLVTSTTPAVTSVTDTIDTTTVKLTATATAAEGGNVVYTATVGAPVTGSPVTVTLSNGQSITIEVGKTSGTVTTTAPNDVLNGHAPLTNAITGVSGGNYENLVADKTPVSTTVTDTVDTTNLSLSATNSVAEGGSITYTATLTNAAGTPVTVTLSNGAVITIEAGKTTGTVTVPAPADDVYKDAGNVQATITNATGGNFENLVTSTTPAVTSVTDTIDTTTVKLTATATAAEGGTVTYTATVGAPVTGSPVIVNLANGQSITIEVGKTTGTVTTIAPNDALTGQAPLTNSITNVSGGNYENLVADKTPVSTTVTDTVDTTNLSLSATGTVAEGGSITYTATLTNAAGSPVTVTLSNGAVITIEAGKTTGTVTVPAPADDVYKDAGNVQATIKSATGGNFENLVTSTTPAVTSVTDTIDTTTVKLTATTTAAEGGTVTYTATVGAPVTGSPVTVNLANGQSITIEVGKTTGTVTTTAPNDALTGHTPLTNSITNVTGGNYENLVADKTPVSTTVTDTIDTTNLSLSATGTVAEGGSITYTATLTNAAGSPVTVTLSNGAVITIDAGKTTGTVTVPAPADDVYKDAGNVQATITNATGGNFENLVTSTTPAVTSVTDTIDTTTVSITGSTSVTEGQTASYTVSLTHPAQTEVTLKIVYSGTAADGSDFTGVYTVKIPAGASSAQFNVATIDDKITEGTENFVVKIDSATGGNFENLAVSSTNGSVSTSIIDNDAPPVIDLDANNSSGATGADYKVTFTEGTTGPGVSIADTDIKITDPDSTMLTGATIVLTNRQDGDALNLGNSVNGITINANSTNGTVTLTLSGNATLADYMQAIKNISFTNSSEDPSTVPRIITVTVTDGGNYSNTATTTVNVVAINDAPVAAPVNVTGTEDTPLILGWSTFGVSDVDSPASSLGVKITQLPGEGKLQYLDGSTWKDVANNQTFSKADIDAGKLRFVPDANESGVNGYGGTGVGNNQADYAQIKFQPTDGQLLGNTGTIKIDITPVADAPTLSVADNSVKSTGLIKEVWTGLSGLGTDGSGANSTTLKNVIDGAGKPNTSGTVNNVQSDGSVTAGTASKTSGLIYLEAGKTYTFSGTGDDSLLVTIGGKSVASTTWGAGGNLNGSFTPTTSGYYTLDIYHHNQSGPGSYDVNLSVNGSTPIDLSSAGVPIYTGVQDLVNAGVTVSDLHGSNGEGYYDGYKLNTGAEGTTVKLSSISTALTDTDGSETLSIKISGAPVGSVLSDGAGHSFTVTATSGDANVTGWNLGTLTVTPPTYYNGQFNLTVTSTSTEQVGGSASTTATIPVTVVPAVYNSIVATSADDTVTGTDGNDIMVADIGGLTVVPGTNYNIAFMVDSSGSMSASSINAAKDSLTSVFNTLKQSLGGSNSGTVNIFLVDFDTQVNKSVSVNLNDPNALTLLKAVLDSMASGGGTNYEDVFKATANFFQSAEAVANTGAKNLTYFITDGQPTYYQSNEQTNPTLYGNVKLDDVVKTSNYKLGSSFATYIDSTHYLSISTSGEVVLQTLKNNNWSWNSLGTIHAQGDGTYEISSLAGSGNSTSSNTTDNSAAGFALLSGLSNVEAIGLNNGVSLNDLKPYDSDKTPQTNIDPKDLANSIIGHTEATMPGNDTVSGGDGNDILFGDLVSFNGIAGEGYQAMQAFVAKETGVDVSKVTTSNVHQYITEHYQAFDVSGAHDGNDTLLGGAGNDILFGSGGNDLLDGGKGNDILLGGTGNDTLIGGQGNDILIGGSGADTFVWKAGDTGNDVIKDFKASEGDRIDLRDLLQGETGSTIDNFLKITTVDGVSSLQVSSGGKFNSGDAAAATPDVTIKLEGNNWSSANIHNLIAGSDPTIKVDHNNS DIP protein Q3KK29 original sequence version: 49 FBCD98C90EEA1FDE q3kk32_psepf lapF Pfl01_0130 protein protein psepf Pseudomonas fluorescens MFFAGLTFTIKRFLLCPGCLALAVRFAIPRIARWLACALLLAGIMLGGLHADWDFSAISRKATALYGPLGAGQQRIDAWQNLLATQKQVSEMEKLKVVNLFFNKQMRYVEDIDLWHEVDYWETPIEALWKGAGDCEDYAIAKYFSLRHLGVASDKLRITYVKALRQNRAHMVLTYYSSPDAMPLVLDSLIDPIKPAAERTDLLPVYSFNAEGLYLPGAKGNKKVGDTKRLSRWQDVLKKMQAEGFPVETTN DIP protein Q3KK32 original sequence version: 39 438AE5C5244F9A51 q3kk31_psepf lapD Pfl01_0131 protein protein psepf Pseudomonas fluorescens MSLFKQLLIAICLFLVVAFTGSFMVSLESSRTQYVNQLRSHAQDAATALALSLTPNIDDPAMVELLVSSIFDSGYYSSIRVVDLKTDQTIVERNGIPAVTNVPDWFVKLIGLEPAGGDALVSRGWEQAARVEVVSHPMFALAKLWQSALGSLGWLLVCGAVSAVLGALLLRRQLKPLDYMVKQSHAIARREFLSLPDLPRTPELRRVVLAMNQMVEKLKALFQEQAERSEKLRTESYQDNLTGLANRRYFEMQLNARVSNPEQASSGYLLLLRVKDLAGLNQRLGGQRTDELLKAVGEQLSRECAKYPETQNLVTRIRGGEFAVLAPGMTREEALQLAQSLDSALSSLYATGATDVAAVASIGLAPFAHGDSPQAVLSLGDQALAQAEGQGEQNWACLDQSLVADVGDDHHAWHRLLDQALNQRRFELFFQPVVAAQDTQLVLHYKVLSRLLDEQGQTIPAGRFLPWLERFGWTARLDRLMLERVLEQMAGHEESLALNLSSATLADPQALNKVFEILRAHSNLGARLTLEIGEEQLPEQAVLEQLTRRLRELGFSLSLQRFGGRFSMIGNLARLGLAYLKIDGSYIRAIDQESDKRLFIEAIQRAAHSIDLPLIAERVETEGELSVIREMGLYGVQGQLFGEPKPWG DIP protein Q3KK31 original sequence version: 42 0EC47D6D9BF3C233 lapd-lapf 1 4 unspecified role unspecified role prey prey Q3KK32:p.Glu136Ala Q3KK32-136-136 mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain E A Wed May 02 20:04:39 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu136ala' Tue Jun 30 16:18:36 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu136Ala' q3kk32-- Q3KK32-- ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Q3KK32:p.Asp134Ala Q3KK32-134-134 mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain D A Wed May 02 20:16:52 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asp134ala' Tue Jun 30 17:06:48 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asp134Ala' 5 unspecified role unspecified role bait bait q3kk31-- Q3KK31-- his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association figure 5 lapa-lapf 2 3 enzyme target enzyme target substrate substrate unspecified role unspecified role region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined n-terminal region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain undetermined undetermined sequence position undetermined 4 enzyme enzyme unspecified role unspecified role Q3KK32:p.Asp134Ala Q3KK32-134-134 mutation disrupting rate mutation disrupting interaction rate mutation disrupting rate certain certain sequence position certain certain certain sequence position certain D A Wed May 02 15:51:28 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asp134ala' Tue Jun 30 19:54:45 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asp134Ala' q3kk32-- Q3KK32-- ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Q3KK32:p.Glu136Ala Q3KK32-136-136 mutation disrupting rate mutation disrupting interaction rate mutation disrupting rate certain certain sequence position certain certain certain sequence position certain E A Wed May 02 18:35:36 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu136ala' Tue Jun 30 19:45:30 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu136Ala' protein cleavage protein cleavage figure 3