IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Fruitless recruits two antagonistic chromatin factors to establish single-neuron sexual dimorphism. Fruitless recruits two antagonistic chromatin factors to establish single-neuron sexual dimorphism. Cell (0092-8674) 2012 imex curation Ito H., Sato K., Koganezawa M., Ote M., Matsumoto K., Hama C., Yamamoto D. Chromatin - Epigenetic interactions resulting in chromatin modulation daichan@m.tohoku.ac.jp 19-Sept-2012: Contacted by IntAct-Help. Only protein-protein interactions drome-schneider_2 Drosophila melanogaster late embryo cells Fruit fly schneider_2 Established from the late embryo of Drosophila melanogaster Mixture of diploid and tetraploid. Originally had some XY cells now predominantly XX. anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Ito H., Sato K., Koganezawa M., Ote M., Matsumoto K., Hama C., Yamamoto D. Chromatin - Epigenetic interactions resulting in chromatin modulation daichan@m.tohoku.ac.jp Accepted 2012-JUL-19 by JYOTI 19-Sept-2012: Contacted by IntAct-Help. Cell (0092-8674) 2012 Only protein-protein interactions imex curation Fruitless recruits two antagonistic chromatin factors to establish single-neuron sexual dimorphism. Fruitless recruits two antagonistic chromatin factors to establish single-neuron sexual dimorphism. Cell (0092-8674) 2012 imex curation Ito H., Sato K., Koganezawa M., Ote M., Matsumoto K., Hama C., Yamamoto D. Chromatin - Epigenetic interactions resulting in chromatin modulation daichan@m.tohoku.ac.jp 19-Sept-2012: Contacted by IntAct-Help. Only protein-protein interactions drome-head Drosophila melanogaster head anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Ito H., Sato K., Koganezawa M., Ote M., Matsumoto K., Hama C., Yamamoto D. Chromatin - Epigenetic interactions resulting in chromatin modulation daichan@m.tohoku.ac.jp Accepted 2012-JUL-19 by JYOTI 19-Sept-2012: Contacted by IntAct-Help. Cell (0092-8674) 2012 Only protein-protein interactions imex curation Fruitless recruits two antagonistic chromatin factors to establish single-neuron sexual dimorphism. Fruitless recruits two antagonistic chromatin factors to establish single-neuron sexual dimorphism. Cell (0092-8674) 2012 imex curation Ito H., Sato K., Koganezawa M., Ote M., Matsumoto K., Hama C., Yamamoto D. Chromatin - Epigenetic interactions resulting in chromatin modulation daichan@m.tohoku.ac.jp 19-Sept-2012: Contacted by IntAct-Help. Only protein-protein interactions drome-larva Drosophila melanogaster larva larva Immature form of an organism anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Ito H., Sato K., Koganezawa M., Ote M., Matsumoto K., Hama C., Yamamoto D. Chromatin - Epigenetic interactions resulting in chromatin modulation daichan@m.tohoku.ac.jp Accepted 2012-JUL-19 by JYOTI 19-Sept-2012: Contacted by IntAct-Help. Cell (0092-8674) 2012 Only protein-protein interactions imex curation Fruitless recruits two antagonistic chromatin factors to establish single-neuron sexual dimorphism. Fruitless recruits two antagonistic chromatin factors to establish single-neuron sexual dimorphism. Cell (0092-8674) 2012 imex curation Ito H., Sato K., Koganezawa M., Ote M., Matsumoto K., Hama C., Yamamoto D. Chromatin - Epigenetic interactions resulting in chromatin modulation daichan@m.tohoku.ac.jp 19-Sept-2012: Contacted by IntAct-Help. Only protein-protein interactions drome-salivary_gland Drosophila melanogaster Salivary gland salivary_gland Salivary gland fluorescence imaging fluorescence microscopy fluorescence imaging immunostaining immunostaining Ito H., Sato K., Koganezawa M., Ote M., Matsumoto K., Hama C., Yamamoto D. Chromatin - Epigenetic interactions resulting in chromatin modulation daichan@m.tohoku.ac.jp Accepted 2012-JUL-19 by JYOTI 19-Sept-2012: Contacted by IntAct-Help. Cell (0092-8674) 2012 Only protein-protein interactions imex curation Fruitless recruits two antagonistic chromatin factors to establish single-neuron sexual dimorphism. Fruitless recruits two antagonistic chromatin factors to establish single-neuron sexual dimorphism. Cell (0092-8674) 2012 imex curation Ito H., Sato K., Koganezawa M., Ote M., Matsumoto K., Hama C., Yamamoto D. Chromatin - Epigenetic interactions resulting in chromatin modulation daichan@m.tohoku.ac.jp 19-Sept-2012: Contacted by IntAct-Help. Only protein-protein interactions drome-schneider_2 Drosophila melanogaster late embryo cells Fruit fly schneider_2 Established from the late embryo of Drosophila melanogaster Mixture of diploid and tetraploid. Originally had some XY cells now predominantly XX. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Ito H., Sato K., Koganezawa M., Ote M., Matsumoto K., Hama C., Yamamoto D. Chromatin - Epigenetic interactions resulting in chromatin modulation daichan@m.tohoku.ac.jp Accepted 2012-JUL-19 by JYOTI 19-Sept-2012: Contacted by IntAct-Help. Cell (0092-8674) 2012 Only protein-protein interactions imex curation hp1_drome Heterochromatin protein 1 Su(var)205 HP1 CG8409 Non-histone chromosomal protein C1A9 antigen protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MGKKIDNPESSAKVSDAEEEEEEYAVEKIIDRRVRKGKVEYYLKWKGYPETENTWEPENNLDCQDLIQQYEASRKDEEKSAASKKDRPSSSAKAKETQGRASSSTSTASKRKSEEPTAPSGNKSKRTTDAEQDTIPVSGSTGFDRGLEAEKILGASDNNGRLTFLIQFKGVDQAEMVPSSVANEKIPRMVIHFYEERLSWYSDNED DIP protein P05205 original sequence version: 93 DIP protein P05205 original sequence version: 157 DIP protein P05205 original sequence version: 117 6A5B204C487526B7 q8in81-6 Sex determination protein fruitless B fru BTB-VI CG14307 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MMATSQDYFGNPYALFRGPPTTLRPRESPLGVGHPHGHGHLHSHAHAHGHGHAHSHYAALDLQTPHKRNIETDVRAPPPPLPPPPLPLPPASPRYNTDQGAMDQQFCLRWNNHPTNLTGVLTSLLQREALCDVTLACEGETVKAHQTILSACSPYFETIFLQNQHPHPIIYLKDVRYSEMRSLLDFMYKGEVNVGQSSLPMFLKTAESLQVRGLTDNNNLNYRSDCDKLRDSAASSPTGRGPSNYTGGLGGAGGVADAMRESRDSLRSRCERDLRDELTQRSSSSMSERSSAAAAAAAAAAAVAAAGGNVNAAAVALGLTTPTGGERSPSVGSASAAAAAAAVAAAVAAAANRSASADGCSDRGSERGTLERTDSRDDLLQLDYSNKDNNNSNSSSTGGNNNNNNNNNNNSSSNNNNSSSNRERNNSGERERERERERERDRDRELSTTPVEQLSSSKRRRKNSSSNCDNSLSSSHQDRHYPQDSQANFKSSPVPKTGGSTSESEDAGGRHDSPLSMTTSVHLGGGGGNVGAASALSGLSQSLSIKQELMDAQQQQQHREHHVALPPDYLPSAALKLHAEDMSTLLTQHALQAADARDEHNDAKQLQLDQTDNIDGSSARHHLSTPLSTSSSASPPPPPFGMHLSAALKREYHPLHYMAAGNGHNGPSALGYGNQGSGNAPNSAGGAGSVAGGVGAGGGAGGATGAAGHNSHHTMSYHNMFTPSRDPGTMWRCRSCGKEVTNRWHHFHSHTAQRSMCPYCPATYSRIDTLRSHLRVKHPDRLLKLNSSI 7F87220F6C723A29 hdac1_drome Histone deacetylase HDAC1 HDAC1 Rpd3 CG7471 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MQSHSKKRVCYYYDSDIGNYYYGQGHPMKPHRIRMTHNLLLNYGLYRKMEIYRPHKATADEMTKFHSDEYVRFLRSIRPDNMSEYNKQMQRFNVGEDCPVFDGLYEFCQLSAGGSVAAAVKLNKQASEICINWGGGLHHAKKSEASGFCYVNDIVLGILELLKYHQRVLYIDIDVHHGDGVEEAFYTTDRVMTVSFHKYGEYFPGTGDLRDIGAGKGKYYAVNIPLRDGMDDDAYESIFVPIISKVMETFQPAAVVLQCGADSLTGDRLGCFNLTVKGHGKCVEFVKKYNLPFLMVGGGGYTIRNVSRCWTYETSVALAVEIANELPYNDYFEYFGPDFKLHISPSNMTNQNTSEYLEKIKNRLFENLRMLPHAPGVQIQAIPEDAINDESDDEDKVDKDDRLPQSDKDKRIVPENEYSDSEDEGEGGRRDNRSYKGQRKRPRLDKDTNSNKASSETSSEIKDEKEKGDGADGEESTASNTNSNNNSNNKSDNDAGATANAGSGSGSGSGAGAKGAKENNI DIP protein Q94517 original sequence version: 67 B0F6503D42A1BCE9 q8in81-9 Sex determination protein fruitless A fru BTB-VI CG14307 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MDQQFCLRWNNHPTNLTGVLTSLLQREALCDVTLACEGETVKAHQTILSACSPYFETIFLQNQHPHPIIYLKDVRYSEMRSLLDFMYKGEVNVGQSSLPMFLKTAESLQVRGLTDNNNLNYRSDCDKLRDSAASSPTGRGPSNYTGGLGGAGGVADAMRESRDSLRSRCERDLRDELTQRSSSSMSERSSAAAAAAAAAAAVAAAGGNVNAAAVALGLTTPTGGERSPSVGSASAAAAAAAVAAAVAAAANRSASADGCSDRGSERGTLERTDSRDDLLQLDYSNKDNNNSNSSSTGGNNNNNNNNNNNSSSNNNNSSSNRERNNSGERERERERERERDRDRELSTTPVEQLSSSKRRRKNSSSNCDNSLSSSHQDRHYPQDSQANFKSSPVPKTGGSTSESEDAGGRHDSPLSMTTSVHLGGGGGNVGAASALSGLSQSLSIKQELMDAQQQQQHREHHVALPPDYLPSAALKLHAEDMSTLLTQHALQAADARDEHNDAKQLQLDQTDNIDGE E91B5D22099FF65B fru_drome Sex determination protein fruitless fru BTB-VI CG14307 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MMATSQDYFGNPYALFRGPPTTLRPRESPLGVGHPHGHGHLHSHAHAHGHGHAHSHYAALDLQTPHKRNIETDVRAPPPPLPPPPLPLPPASPRYNTDQGAMDQQFCLRWNNHPTNLTGVLTSLLQREALCDVTLACEGETVKAHQTILSACSPYFETIFLQNQHPHPIIYLKDVRYSEMRSLLDFMYKGEVNVGQSSLPMFLKTAESLQVRGLTDNNNLNYRSDCDKLRDSAASSPTGRGPSNYTGGLGGAGGVADAMRESRDSLRSRCERDLRDELTQRSSSSMSERSSAAAAAAAAAAAVAAAGGNVNAAAVALGLTTPTGGERSPSVGSASAAAAAAAVAAAVAAAANRSASADGCSDRGSERGTLERTDSRDDLLQLDYSNKDNNNSNSSSTGGNNNNNNNNNNNSSSNNNNSSSNRERNNSGERERERERERERDRDRELSTTPVEQLSSSKRRRKNSSSNCDNSLSSSHQDRHYPQDSQANFKSSPVPKTGGSTSESEDAGGRHDSPLSMTTSVHLGGGGGNVGAASALSGLSQSLSIKQELMDAQQQQQHREHHVALPPDYLPSAALKLHAEDMSTLLTQHALQAADARDEHNDAKQLQLDQTDNIDGRVKCFNIKHDRHPDRELDRNHREHDDDPGVIEEVVVDHVREMEAGNEHDPEEMKEAAYHATPPKYRRAVVYAPPHPDEEAASGSGSDIYVDGGYNCEYKCKELNMRAIRCSRQQHMMSHYSPHHPHHRSLIDCPAEAAYSPPVANNQAYLASNGAVQQLDLSTYHGHANHQLHQHPPSATHPSHSQSSPHYPSASGAGAGAGSVSVSIAGSASGSATSAPASVATSAVSPQPSSSSTGSTSSAAAVAAAAAAAANRRDHNIDYSTLFVQLSGTLPTLYRCVSCNKIVSNRWHHANIHRPQSHECPVCGQKFTRRDNMKAHCKIKHADIKDRFFSHYVHM E2A6005D5A7FB7B7 q8in81-1 Sex determination protein fruitless E fru BTB-VI CG14307 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MMATSQDYFGNPYALFRGPPTTLRPRESPLGVGHPHGHGHLHSHAHAHGHGHAHSHYAALDLQTPHKRNIETDVRAPPPPLPPPPLPLPPASPRYNTDQGAMDQQFCLRWNNHPTNLTGVLTSLLQREALCDVTLACEGETVKAHQTILSACSPYFETIFLQNQHPHPIIYLKDVRYSEMRSLLDFMYKGEVNVGQSSLPMFLKTAESLQVRGLTDNNNLNYRSDCDKLRDSAASSPTGRGPSNYTGGLGGAGGVADAMRESRDSLRSRCERDLRDELTQRSSSSMSERSSAAAAAAAAAAAVAAAGGNVNAAAVALGLTTPTGGERSPSVGSASAAAAAAAVAAAVAAAANRSASADGCSDRGSERGTLERTDSRDDLLQLDYSNKDNNNSNSSSTGGNNNNNNNNNNNSSSNNNNSSSNRERNNSGERERERERERERDRDRELSTTPVEQLSSSKRRRKNSSSNCDNSLSSSHQDRHYPQDSQANFKSSPVPKTGGSTSESEDAGGRHDSPLSMTTSVHLGGGGGNVGAASALSGLSQSLSIKQELMDAQQQQQHREHHVALPPDYLPSAALKLHAEDMSTLLTQHALQAADARDEHNDAKQLQLDQTDNIDGRVKCFNIKHDRHPDRELDRNHREHDDDPGVIEEVVVDHVREMEAGNEHDPEEMKEAAYHATPPKYRRAVVYAPPHPDEEAASGSGSDIYVDGGYNCEYKCKELNMRAIRCSRQQHMMSHYSPHHPHHRSLIDCPAEAAYSPPVANNQAYLASNGAVQQLDLSTYHGHANHQLHQHPPSATHPSHSQSSPHYPSASGAGAGAGSVSVSIAGSASGSATSAPASVATSAVSPQPSSSSTGSTSSAAAVAAAAAAAANRRDHNIDYSTLFVQLSGTLPTLYRCVSCNKIVSNRWHHANIHRPQSHECPVCGQKFTRRDNMKAHCKIKHADIKDRFFSHYVHM E2A6005D5A7FB7B7 q9vdk5_drome bon CG15687 CG5206 0241 0249 0434 0487 06 08 12 150268_at 150269_at 20 Bon Bonus Dmel\CG5206 anon-WO0118547.418 l(3)S043420 Dmel_CG5206 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MDMDLEQLKNDFLPLIAGIKQEQLDAVPTDALPQMSTPNTASGAPTTSSLSSSSLSLSNPCDSAEKRSESNSSASAKFTLFKCVYCAQLLGSNDRPKLLECLHVACAQCVSTKFSELDRSLPPLIHCPVCDNASQQEFIVDNQFLIEQCTAGDSGDGVGLLGLTGEGQKSSAAASIQCSSCSDGAVATSWCVDCSEYICDSCVQAHQRLKITKDHTIKPKDEANNEQLAGAAGVDKLHMCQLHPQEKLSLFCETCDKLTCRDCQLSDHRDHKYKFAHEIATESRQALSTLVSEINYKRFLLSSATKVIDDRQQLIHDKKKDLIKEITAMAVKITNTVNTRGKQLIMRLNEVCDSKLKVLVEKKETLQLLSDNTDHCIDFMQNALEKGSDFAILSSKKSLVRHLQKLKCQRADIPNPEIPVRIQVQLNQVSDLQKVISQLGIIIVDGKPYPPTPSPNGTPQPQHPPRQPPSPNMAPPLRPGLPPGMPAGLSPNGPPVNFGPQNGPPLYSNAAQQQFNNLSMSRSFPGDGSGKVRFGGMPPVGMQRHGQPHVSSSTHPQNMDISLRGLLNNQAAQSPNAHMAFNGPPSYPGGPQGAPAPAHQPMGPQMRPHFMPGQQGFSQGGGPGGGPRDANFMNSNARFQSQYQRMANHAQQAAAAAAMAGAAGGGGGQIPSPGALQRPQMMSNPMQNVSAMQNSLGFHGSQAGFNTGPPQTSPQLGGGGMHSLAKWHIPQSAQQSNMCSQQGPLLPFANGRQTSENFKISLKSPNTLKNSTPPSLGGGGAGHQGHGNGSSSAQLNAAALGLGPAVSILSNVTSTNPKTPSPSTHENTKDFTEPIDKVRDDSINDLIATIAKLDSNGVQVLPEGRTKTTSPQVHSSTDLSNTQEVNNKNEQKDDPNEDWCAVCLDGGELMCCDKCPKVFHQNCHIPAISSLPDESESWQCLLCVNIKELTKTEGSEKSSSGELSALELRILQRICLELYCQYEQSLNFREPESPANTSYYEIVSSPMSLDVIRTRLDPSSPNHYKDIAGFVSDVRLIFSNTYLFYQEDTKTYSNAKYLENFFEEQLAKWLPQFEGTKPQGKRNTSNSPALLGVNATGSPSPIENGRKSCGSASLGDSDGACLPAKRARRSAHE 853F7EE9793A7D60 fru-bon 2 6 unspecified role unspecified role prey prey 8 unspecified role unspecified role prey prey 12 unspecified role unspecified role prey prey 11 unspecified role unspecified role bait bait association association Fig. 3B-D Heat-shock The proportion of Fru-expressing neurons represents less than 2% of all neurons in the brain. To increase the sensitivity of detection, heat-shockable-fruEM was overexpressed in adult flies fru-bon-2 5 7 unspecified role unspecified role bait bait 12 unspecified role unspecified role prey prey 8 unspecified role unspecified role prey prey 6 unspecified role unspecified role prey prey association association Fig. 3F fru-rpd3-1 5 6 unspecified role unspecified role prey prey 12 unspecified role unspecified role ancillary ancillary 7 unspecified role unspecified role bait bait 8 unspecified role unspecified role prey prey association association Fig. 3G, 3H fru-rpd3-2 5 6 unspecified role unspecified role prey prey 8 competitor competitor competitor prey prey 7 unspecified role unspecified role bait bait association association Fig. 3I Heat-shock bon also present but not shown to be required for the interaction fru-rpd3-3 5 7 unspecified role unspecified role bait bait 12 unspecified role unspecified role ancillary ancillary 6 unspecified role unspecified role prey prey association association Fig. 3I bon-fru 1 12 unspecified role unspecified role bait bait region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 11 unspecified role unspecified role prey prey physical association physical association Supp. Fig. 2B bon-fru-1 1 12 unspecified role unspecified role bait bait region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 9 unspecified role unspecified role prey prey physical association physical association Supp. Fig. 2B bon-fru-2 1 12 unspecified role unspecified role bait bait region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 7 unspecified role unspecified role prey prey physical association physical association Supp. Fig. 2B rpd3-bon 3 8 unspecified role unspecified role bait bait 12 unspecified role unspecified role prey prey physical association physical association Supp. Fig. 2C su_var_205-bon 1 6 unspecified role unspecified role bait bait region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 12 unspecified role unspecified role prey prey physical association physical association Supp. Fig. 2D bon-fru-3 4 10 unspecified role unspecified role neutral component neutral component 12 unspecified role unspecified role neutral component neutral component 8 unspecified role unspecified role neutral component neutral component colocalization colocalization Fig. 4A, 4B Co-localization not observed in bon-/- cells bon-fru-5 4 6 unspecified role unspecified role neutral component neutral component 10 unspecified role unspecified role neutral component neutral component 12 unspecified role unspecified role neutral component neutral component colocalization colocalization Fig. 4A, 4B Co-localization not observed in bon-/- cells