MINT MINT, Dpt of Biology, University of Rome Tor Vergata MINT http://mint.bio.uniroma2.it/mint url>http://mint.bio.uniroma2.it/mint/ https://mint.bio.uniroma2.it MINT-[0-9]+ https://mint.bio.uniroma2.it/index.php/results-interactions/?id=${ac} The Molecular INTeraction database (MINT) is a relational database designed to store interactions between biological molecules. http://mint.bio.uniroma2.it Insights in small Heat Shock Protein/client interaction by combined protection analysis of two different client proteins. Insights in small Heat Shock Protein/client interaction by combined protection analysis of two different client proteins. FEBS Lett. (0014-5793) 2012 Eisenhardt BD., Forreiter C. suggested by lperfetto Benjamin.Eisenhardt@bio.uni-giessen.de in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Eisenhardt BD., Forreiter C. suggested by lperfetto Benjamin.Eisenhardt@bio.uni-giessen.de FEBS Lett. (0014-5793) 2012 hs17b_arath 17.6 kDa class I heat shock protein 2 HSP17.6B 17.6 kDa heat shock protein 2 F16P2.12 At2g29500 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MSMIPSFFNNNRRSNIFDPFSLDVWDPFKELTSSSLSRENSAIVNARVDWRETPEAHVFKADLPGLKKEEVKVEIEEDSVLKISGERHVEKEDKNDTWHRVERSSGQFTRRFRLPENVKMDQVKAAMENGVLTVTVPKAETKKADVKSIQISG mint 896A13837D301B48 phy1_syny3 Phytochrome-like protein Cph1 Light-regulated histidine kinase 1 cph1 slr0473 Bacteriophytochrome Cph1 protein protein syny3 Synechocystis sp. (strain PCC 6803 / Kazusa) MATTVQLSDQSLRQLETLAIHTAHLIQPHGLVVVLQEPDLTISQISANCTGILGRSPEDLLGRTLGEVFDSFQIDPIQSRLTAGQISSLNPSKLWARVMGDDFVIFDGVFHRNSDGLLVCELEPAYTSDNLPFLGFYHMANAALNRLRQQANLRDFYDVIVEEVRRMTGFDRVMLYRFDENNHGDVIAEDKRDDMEPYLGLHYPESDIPQPARRLFIHNPIRVIPDVYGVAVPLTPAVNPSTNRAVDLTESILRSAYHCHLTYLKNMGVGASLTISLIKDGHLWGLIACHHQTPKVIPFELRKACEFFGRVVFSNISAQEDTETFDYRVQLAEHEAVLLDKMTTAADFVEGLTNHPDRLLGLTGSQGAAICFGEKLILVGETPDEKAVQYLLQWLENREVQDVFFTSSLSQIYPDAVNFKSVASGLLAIPIARHNFLLWFRPEVLQTVNWGGDPNHAYEATQEDGKIELHPRQSFDLWKEIVRLQSLPWQSVEIQSALALKKAIVNLILRQAEELAQLARNLERSNADLKKFAYIASHDLQEPLNQVSNYVQLLEMRYSEALDEDAKDFIDFAVTGVSLMQTLIDDILTYAKVDTQYAQLTFTDVQEVVDKALANLKQRIEESGAEIEVGSMPAVMADQIQLMQVFQNLIANGIKFAGDKSPKIKIWGDRQEDAWVFAVQDNGIGIDPQFFERIFVIFQRLHTRDEYKGTGMGLAICKKIIEGHQGQIWLESNPGEGSTFYFSIPIGN DIP protein Q55168 original sequence version: 128 A9ECA6D8D5B3C88A mdhm_pig Malate dehydrogenase, mitochondrial MDH2 protein protein pig Sus scrofa (Pig) Pig MLSALARPAGAALRRSFSTSXQNNAKVAVLGASGGIGQPLSLLLKNSPLVSRLTLYDIAHTPGVAADLSHIETRATVKGYLGPEQLPDCLKGCDVVVIPAGVPRKPGMTRDDLFNTNATIVATLTAACAQHCPDAMICIISNPVNSTIPITAEVFKKHGVYNPNKIFGVTTLDIVRANAFVAELKGLDPARVSVPVIGGHAGKTIIPLISQCTPKVDFPQDQLSTLTGRIQEAGTEVVKAKAGAGSATLSMAYAGARFVFSLVDAMNGKEGVVECSFVKSQETDCPYFSTPLLLGKKGIEKNLGIGKISPFEEKMIAEAIPELKASIKKGEEFVKNMK B0435E87383D0B1E hs178_arath 17.8 kDa class I heat shock protein HSP17.8 17.8 kDa heat shock protein F22G5.25 At1g07400 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MSLIPSFFGNNRRSNSIFDPFSLDVWDPFKELQFPSSLSGETSAITNARVDWKETAEAHVFKADLPGMKKEEVKVEIEDDSVLKISGERHVEKEEKQDTWHRVERSSGQFSRKFKLPENVKMDQVKASMENGVLTVTVPKVEEAKKKAQVKSIDISG A27A9D2191ABB8E6 cph1-hsp17_8 1 CPH1Δ2 3 predetermined predetermined participant predetermined predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified binding site binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain HSP17.8 5 predetermined predetermined participant predetermined predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified direct interaction direct interaction mint f3a f3b Co-incubation of sHSPs with CPH1Δ2 did not result in interactions at 25 °C (Fig. 3A). A shift in their separation pattern was observed at 50 °C, also visible in the collected fractions (Fig. 3B). At 55 °C the observed sHSP / client complex formation was still detectable. cph1-1 1 CPH1Δ2 3 predetermined predetermined participant predetermined predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified binding site binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain Stoichiometry: 2.0 direct interaction direct interaction f3 mint At 25 °C, CPH1Δ2 eluted at 130 kD (dimer) with another portion around 65 kD (monomers). hsp17_8-mdh2 1 MDH 4 predetermined predetermined participant predetermined predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified HSP17.8 5 predetermined predetermined participant predetermined predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified direct interaction direct interaction sf2 mint The described change in client separation pattern at denaturing conditions was also visible in MDH / At17.8 co-incubations at 45°C (supplementary Fig. 2A). MDH incubated alone at 45 °C resulted in a slight decrease of MDH in the collected fractions compared to untreated samples. At17.6B behaved like At17.8 regarding the visible protection mechanism of MDH and the newly introduced client (supplementary Fig. 2). hsp17_6b-mdh2 1 HSP17.6B 2 predetermined predetermined participant predetermined predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified MDH 4 predetermined predetermined participant predetermined predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified direct interaction direct interaction sf2 mint The described change in client separation pattern at denaturing conditions was also visible in MDH / At17.8 co-incubations at 45°C (supplementary Fig. 2A). MDH incubated alone at 45 °C resulted in a slight decrease of MDH in the collected fractions compared to untreated samples. At17.6B behaved like At17.8 regarding the visible protection mechanism of MDH and the newly introduced client (supplementary Fig. 2). cph1-hsp17_6b 1 HSP17.6 2 predetermined predetermined participant predetermined predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified CPH1Δ2 3 predetermined predetermined participant predetermined predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified binding site binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain direct interaction direct interaction sf2b mint