MINT MINT, Dpt of Biology, University of Rome Tor Vergata MINT http://mint.bio.uniroma2.it/mint url>http://mint.bio.uniroma2.it/mint/ https://mint.bio.uniroma2.it MINT-[0-9]+ https://mint.bio.uniroma2.it/index.php/results-interactions/?id=${ac} The Molecular INTeraction database (MINT) is a relational database designed to store interactions between biological molecules. http://mint.bio.uniroma2.it Tetramerization of human guanylate-binding protein 1 is mediated by coiled-coil formation of the C-terminal α-helices. Tetramerization of human guanylate-binding protein 1 is mediated by coiled-coil formation of the C-terminal α-helices. FEBS J. (1742-464X) 2012 Syguda A., Bauer M., Benscheid U., Ostler N., Naschberger E., Ince S., Stürzl M., Herrmann C. yeast Saccharomyces cerevisiae Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Syguda A., Bauer M., Benscheid U., Ostler N., Naschberger E., Ince S., Stürzl M., Herrmann C. FEBS J. (1742-464X) 2012 Tetramerization of human guanylate-binding protein 1 is mediated by coiled-coil formation of the C-terminal α-helices. Tetramerization of human guanylate-binding protein 1 is mediated by coiled-coil formation of the C-terminal α-helices. FEBS J. (1742-464X) 2012 Syguda A., Bauer M., Benscheid U., Ostler N., Naschberger E., Ince S., Stürzl M., Herrmann C. in vitro In vitro crosslink cross-linking study crosslink predetermined predetermined participant predetermined Syguda A., Bauer M., Benscheid U., Ostler N., Naschberger E., Ince S., Stürzl M., Herrmann C. FEBS J. (1742-464X) 2012 Tetramerization of human guanylate-binding protein 1 is mediated by coiled-coil formation of the C-terminal α-helices. Tetramerization of human guanylate-binding protein 1 is mediated by coiled-coil formation of the C-terminal α-helices. FEBS J. (1742-464X) 2012 Syguda A., Bauer M., Benscheid U., Ostler N., Naschberger E., Ince S., Stürzl M., Herrmann C. in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Syguda A., Bauer M., Benscheid U., Ostler N., Naschberger E., Ince S., Stürzl M., Herrmann C. FEBS J. (1742-464X) 2012 gbp1_human Guanylate-binding protein 1 GBP1 GTP-binding protein 1 Guanine nucleotide-binding protein 1 Interferon-induced guanylate-binding protein 1 protein protein human Homo sapiens Human MASEIHMTGPMCLIENTNGRLMANPEALKILSAITQPMVVVAIVGLYRTGKSYLMNKLAGKKKGFSLGSTVQSHTKGIWMWCVPHPKKPGHILVLLDTEGLGDVEKGDNQNDSWIFALAVLLSSTFVYNSIGTINQQAMDQLYYVTELTHRIRSKSSPDENENEVEDSADFVSFFPDFVWTLRDFSLDLEADGQPLTPDEYLTYSLKLKKGTSQKDETFNLPRLCIRKFFPKKKCFVFDRPVHRRKLAQLEKLQDEELDPEFVQQVADFCSYIFSNSKTKTLSGGIQVNGPRLESLVLTYVNAISSGDLPCMENAVLALAQIENSAAVQKAIAHYEQQMGQKVQLPTETLQELLDLHRDSEREAIEVFIRSSFKDVDHLFQKELAAQLEKKRDDFCKQNQEASSDRCSALLQVIFSPLEEEVKAGIYSKPGGYRLFVQKLQDLKKKYYEEPRKGIQAEEILQTYLKSKESMTDAILQTDQTLTEKEKEIEVERVKAESAQASAKMLQEMQRKNEQMMEQKERSYQEHLKQLTEKMENDRVQLLKEQERTLALKLQEQEQLLKEGFQKESRIMKNEIQDLQTKMRRRKACTIS DIP protein P32455 original sequence version: 122 3A06741218360732 gbp1-gbp1 1 hGBP1 4 predetermined predetermined participant predetermined predetermined predetermined participant predetermined unspecified role unspecified role prey prey over-expressed over expressed level over-expressed living cell living cell fusion protein fusion protein fusion protein undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined binding site binding region binding-associated region binding region deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain hGBP1 4 predetermined predetermined participant predetermined predetermined predetermined participant predetermined unspecified role unspecified role bait bait over-expressed over expressed level over-expressed living cell living cell fusion protein fusion protein fusion protein undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association t1 homomint domino mint gbp1-gbp1-1 1 hGBP1 4 predetermined predetermined participant predetermined predetermined predetermined participant predetermined unspecified role unspecified role bait bait over-expressed over expressed level over-expressed living cell living cell fusion protein fusion protein fusion protein undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined hGBP1 4 predetermined predetermined participant predetermined predetermined predetermined participant predetermined unspecified role unspecified role prey prey over-expressed over expressed level over-expressed living cell living cell binding site binding region binding-associated region binding region deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain fusion protein fusion protein fusion protein undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association t1 homomint mint gbp1-gbp1-2 1 hGBP1 4 predetermined predetermined participant predetermined predetermined predetermined participant predetermined unspecified role unspecified role bait bait over-expressed over expressed level over-expressed living cell living cell binding site binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain fusion protein fusion protein fusion protein undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined hGBP1 4 predetermined predetermined participant predetermined predetermined predetermined participant predetermined unspecified role unspecified role prey prey over-expressed over expressed level over-expressed living cell living cell binding site binding region binding-associated region binding region deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain fusion protein fusion protein fusion protein undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association t1 homomint mint gbp1-3 2 hGBP1 4 weight by staining molecular weight estimation by staining weight by staining predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified binding site binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain Stoichiometry: 2.0 direct interaction direct interaction f2 mint gbp1-3 3 hGBP1 4 predetermined predetermined participant predetermined predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified binding site binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined [EBI-7880817]The shifted ranges (1-481) couldn't be applied as the new full feature sequence is different from the previous one. [EBI-7880817]1-481 [EBI-7880817]P32455,1 Stoichiometry: 2.0 direct interaction direct interaction f3 mint Dimer formation is observed after addition of GppNHp whereas in the presence of GDP and aluminum fluoride hGBP1 f.l. elutes as a protein tetramer (Fig. 3A) Deletion of C-terminal helices α12/13 shows an interesting change in this picture (Fig. 3B). While the protein is still a monomer in the nucleotide-free form (Fig. 3B, solid line) and a dimer in the presence of GppNHp (Fig. 3B, dashed line), only dimer formation is observed in complex with GDP.AlFx (Fig. 3B, dotted line). This can be explained by dimer formation through the LG domain and the necessity of the C-terminal subdomain α12/13 for the establishment of a tetramer. gbp1-3 3 hGBP1 4 predetermined predetermined participant predetermined predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified Stoichiometry: 4.0 direct interaction direct interaction f3a mint Dimer formation is observed after addition of GppNHp whereas in the presence of GDP and aluminum fluoride hGBP1 f.l. elutes as a protein tetramer (Fig. 3A)