MINT MINT, Dpt of Biology, University of Rome Tor Vergata MINT http://mint.bio.uniroma2.it/mint url>http://mint.bio.uniroma2.it/mint/ https://mint.bio.uniroma2.it MINT-[0-9]+ https://mint.bio.uniroma2.it/index.php/results-interactions/?id=${ac} The Molecular INTeraction database (MINT) is a relational database designed to store interactions between biological molecules. http://mint.bio.uniroma2.it The inner membrane histidine kinase EnvZ senses osmolality via helix-coil transitions in the cytoplasm. The inner membrane histidine kinase EnvZ senses osmolality via helix-coil transitions in the cytoplasm. EMBO J. (0261-4189) 2012 imex curation Wang LC., Morgan LK., Godakumbura P., Kenney LJ., Anand GS. Only protein-protein interactions in vitro In vitro protein kinase assay protein kinase assay experimental particp experimental participant identification experimental particp Wang LC., Morgan LK., Godakumbura P., Kenney LJ., Anand GS. EMBO J. (0261-4189) 2012 Only protein-protein interactions imex curation envz_ecoli Sensor histidine kinase EnvZ Osmolarity sensor protein EnvZ envZ ompB perA tpo b3404 JW3367 protein protein ecoli Escherichia coli (strain K12) MRRLRFSPRSSFARTLLLIVTLLFASLVTTYLVVLNFAILPSLQQFNKVLAYEVRMLMTDKLQLEDGTQLVVPPAFRREIYRELGISLYSNEAAEEAGLRWAQHYEFLSHQMAQQLGGPTEVRVEVNKSSPVVWLKTWLSPNIWVRVPLTEIHQGDFSPLFRYTLAIMLLAIGGAWLFIRIQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTRAFNHMAAGVKQLADDRTLLMAGVSHDLRTPLTRIRLATEMMSEQDGYLAESINKDIEECNAIIEQFIDYLRTGQEMPMEMADLNAVLGEVIAAESGYEREIETALYPGSIEVKMHPLSIKRAVANMVVNAARYGNGWIKVSSGTEPNRAWFQVEDDGPGIAPEQRKHLFQPFVRGDSARTISGTGLGLAIVQRIVDNHNGMLELGTSERGGLSIRAWLPVPVTRAQGTTKEG DIP protein P0AEJ4 original sequence version: 89 DIP protein P0AEJ4 original sequence version: 50 D58444D038722146 envz-4 1 envZ 2 weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra experimental particp experimental participant identification experimental particp self self self self purified purified binding site binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined [EBI-7837559]C-terminal positions must always be equal to the length of the protein sequence. Actual positions : 180-180, sequence length 450 [EBI-7837559]180-450 phosphorylation phosphorylation reaction phosphorylation true mint f4 sf3 In order to correlate the decrease in exchange of the His243- containing peptide at high osmolality (Figure 2) with EnvZ signalling, we examined the effect of increasing osmolality on EnvZc autophosphorylation (Figure 4). Surprisingly, there was a dramatic enhancement of autophosphorylation with increasing NaCl concentration (compare top and bottom two panels in Figure 4A; densitometric analysis is shown in Figure 4B). An explanation of this result is that increased H-bonding within the helix containing His243 as observed by decreased deuterium exchange rates (Figure 2) results in increased autophosphorylation. This higher level of EnvZcBP would result in higher levels of OmpRBP, and increased OmpC expression (Nara et al, 1986). Similar effects were observed with sucrose (Supplementary Figure S3A and B). true