IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Hikeshi, a nuclear import carrier for hsp70s, protects cells from heat shock-induced nuclear damage. Hikeshi, a nuclear import carrier for hsp70s, protects cells from heat shock-induced nuclear damage. Cell (0092-8674) 2012 imex curation Kose S., Furuta M., Imamoto N. Bead Halo assay which observes by microscopy a soluble, fluorescently labeled macromolecule binding to the surface of microbeads, onto which a second macromolecule had been pre-immobilized via an affinity tag. The visual appearance of the surface-bound, fluorescently labeled macromolecules is as a bright circle or halo around microscopic cross-sections of beads, which reflects the location on the bead surface where the affinity-tagged protein is immobilized. skose@riken.jp, nimamoto@riken.jp Only protein-protein interactions in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Kose S., Furuta M., Imamoto N. Bead Halo assay which observes by microscopy a soluble, fluorescently labeled macromolecule binding to the surface of microbeads, onto which a second macromolecule had been pre-immobilized via an affinity tag. The visual appearance of the surface-bound, fluorescently labeled macromolecules is as a bright circle or halo around microscopic cross-sections of beads, which reflects the location on the bead surface where the affinity-tagged protein is immobilized. skose@riken.jp, nimamoto@riken.jp Accepted 2012-JUN-14 by JYOTI Cell (0092-8674) 2012 Only protein-protein interactions imex curation Hikeshi, a nuclear import carrier for hsp70s, protects cells from heat shock-induced nuclear damage. Hikeshi, a nuclear import carrier for hsp70s, protects cells from heat shock-induced nuclear damage. Cell (0092-8674) 2012 imex curation Kose S., Furuta M., Imamoto N. Bead Halo assay which observes by microscopy a soluble, fluorescently labeled macromolecule binding to the surface of microbeads, onto which a second macromolecule had been pre-immobilized via an affinity tag. The visual appearance of the surface-bound, fluorescently labeled macromolecules is as a bright circle or halo around microscopic cross-sections of beads, which reflects the location on the bead surface where the affinity-tagged protein is immobilized. skose@riken.jp, nimamoto@riken.jp Only protein-protein interactions in vitro In vitro pull down pull down tag fluorescence tag visualisation by fluorescence tag fluorescence Kose S., Furuta M., Imamoto N. Bead Halo assay which observes by microscopy a soluble, fluorescently labeled macromolecule binding to the surface of microbeads, onto which a second macromolecule had been pre-immobilized via an affinity tag. The visual appearance of the surface-bound, fluorescently labeled macromolecules is as a bright circle or halo around microscopic cross-sections of beads, which reflects the location on the bead surface where the affinity-tagged protein is immobilized. skose@riken.jp, nimamoto@riken.jp Accepted 2012-JUN-14 by JYOTI Cell (0092-8674) 2012 Only protein-protein interactions imex curation nu153_rat Nuclear pore complex protein Nup153 Nup153 153 kDa nucleoporin Nucleoporin Nup153 protein protein rat Rattus norvegicus (Rat) Rat MASGAGGIGGGGGGGKIRTRRCHQGPVKPYQQGRPQHQGILSRVTESVKNIVPGWLQRYFNKSENACSCSVNADEVPRWPENREDEREIYVDENTNTDDGRTTPEPTGSNTEEPSTTSTASNYPDVLTRPSLHRSHLNFSVLESPALHCQPSTSSAFPIGSSGFSLVKEIKDSTSQHDDDNISTTSGFSSRASEKDIAVSKNTSLPPLWSPEAERSHSLSQHTAISSKKPAFNLSAFGTLSTSLGNSSILKTSQLGDSPFYPGKTTYGGAAAAVRQNKVRSTPYQAPVRRQMKAKQLNAQSYGVTSSTARRILQSLEKMSSPLADAKRIPSAVSSPLNSPLDRSGIDSTVFQAKKEKVDSQYPPVQRLMTPKPVSIATNRTVYFKPSLTPSGDLRKTNQRIDKKNSTVDEKNISRQNREQESGFSYPNFSIPAANGLSSGVGGGGGKMRRERTTHFVASKPSEEEEVEVPLLPQISLPISSSSLPTFNFSSPAISAASSSSVSPSQPLSNKVQMTSLGSTGNPVFTFSSPIVKSTQADVLPPASIGFTFSVPLAKTELSGPNSSSETVLSSSVTAQDNTVVNSSSSKKRSAPCEDPFTPAKILREGSVLDILKTPGFMSPKVDSPALQPTTTSSIVYTRPAISTFSSSGVEFGESLKAGSSWQCDTCLLQNKVTDNKCIACQAAKLPLKETAKQTGIGTPSKSDKPASTSGTGFGDKFKPAIGTWDCDTCLVQNKPEAVKCVACETPKPGTGVKRALPLTVASESPVTASSSTTVTTGTLGFGDKFKRPVGSWECPVCCVSNKAEDSRCVSCTSEKPGLVSASSSNSVPVSLPSGGCLGLDKFKKPEGSWDCEVCLVQNKADSTKCIACESAKPGTKSEFKGFGTSSSLNPAPSAFKFGIPSSSSGLSQTFTSTGNFKFGDQGGFKLGTSSDSGSTNTMNTNFKFPKPTGDFKFGVLPDSKPEEIKNDSKNDNFQFGPSSGLSNPASSAPFQFGVSTLGQQEKKEELPQSSSAGFSFGAGVANPSSAAIDTTVTSENKSGFNFGTIDTKSVSVTPFTYKTTEAKKEDASATKGGFTFGKVDSAALSSPSMFVLGRTEEKQQEPVTSTSLVFGKKADNEEPKCQPVFSFGNSEQTKDESSSKPTFSFSVAKPSVKESDQLAKATFAFGNQTNTTTDQGAAKPAFSFLNSSSSSSSTPATSSSASIFGSSTSSSSPPVAAFVFGQASNPVSSSAFGNSAESSTSQPLLFPQDGKPATTSSTASAAPPFVFGTGASSNSTVSSGFTFGATTTSSSSGSFFVFGTGHSAPSASPAFGANQTPTFGQSQGASQPNPPSFGSISSSTALFSAGSQPVPPPTFGTVSSSSQPPVFGQQPSQSAFGSGTANASSVFQFGSSTTNFNFTNNNPSGVFTFGASPSTPAAAAQPSGSGGFSFSQSPASFTVGSNGKNMFSSSGTSVSGRKIKTAVRRKK DIP protein P49791 original sequence version: 58 C3DFD9697C556A7C hikes_human Protein Hikeshi HIKESHI C11orf73 HSPC138 HSPC179 HSPC248 protein protein human Homo sapiens Human MFGCLVAGRLVQTAAQQVAEDKFVFDLPDYESINHVVVFMLGTIPFPEGMGGSVYFSYPDSNGMPVWQLLGFVTNGKPSAIFKISGLKSGEGSQHPFGAMNIVRTPSVAQIGISVELLDSMAQQTPVGNAAVSSVDSFTQFTQKMLDNFYNFASSFAVSQAQMTPSPSEMFIPANVVLKWYENFQRRLAQNPLFWKT 4FA524439B630511 hsp7c_human Heat shock cognate 71 kDa protein HSPA8 HSC70 HSP73 HSPA10 Heat shock 70 kDa protein 8 Lipopolysaccharide-associated protein 1 protein protein human Homo sapiens Human MSKGPAVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNPTNTVFDAKRLIGRRFDDAVVQSDMKHWPFMVVNDAGRPKVQVEYKGETKSFYPEEVSSMVLTKMKEIAEAYLGKTVTNAVVTVPAYFNDSQRQATKDAGTIAGLNVLRIINEPTAAAIAYGLDKKVGAERNVLIFDLGGGTFDVSILTIEDGIFEVKSTAGDTHLGGEDFDNRMVNHFIAEFKRKHKKDISENKRAVRRLRTACERAKRTLSSSTQASIEIDSLYEGIDFYTSITRARFEELNADLFRGTLDPVEKALRDAKLDKSQIHDIVLVGGSTRIPKIQKLLQDFFNGKELNKSINPDEAVAYGAAVQAAILSGDKSENVQDLLLLDVTPLSLGIETAGGVMTVLIKRNTTIPTKQTQTFTTYSDNQPGVLIQVYEGERAMTKDNNLLGKFELTGIPPAPRGVPQIEVTFDIDANGILNVSAVDKSTGKENKITITNDKGRLSKEDIERMVQEAEKYKAEDEKQRDKVSSKNSLESYAFNMKATVEDEKLQGKINDEDKQKILDKCNEIINWLDKNQTAEKEEFEHQQKELEKVCNPIITKLYQSAGGMPGGMPGGFPGGGAPPSGGASSGPTIEEVD 9AA27B210730670C hsp71_human Heat shock 70 kDa protein 1A/1B HSPA1A HSPA1 HSPA1B Heat shock 70 kDa protein 1/2 HSX70 protein protein human Homo sapiens Human MAKAAAIGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVALNPQNTVFDAKRLIGRKFGDPVVQSDMKHWPFQVINDGDKPKVQVSYKGETKAFYPEEISSMVLTKMKEIAEAYLGYPVTNAVITVPAYFNDSQRQATKDAGVIAGLNVLRIINEPTAAAIAYGLDRTGKGERNVLIFDLGGGTFDVSILTIDDGIFEVKATAGDTHLGGEDFDNRLVNHFVEEFKRKHKKDISQNKRAVRRLRTACERAKRTLSSSTQASLEIDSLFEGIDFYTSITRARFEELCSDLFRSTLEPVEKALRDAKLDKAQIHDLVLVGGSTRIPKVQKLLQDFFNGRDLNKSINPDEAVAYGAAVQAAILMGDKSENVQDLLLLDVAPLSLGLETAGGVMTALIKRNSTIPTKQTQIFTTYSDNQPGVLIQVYEGERAMTKDNNLLGRFELSGIPPAPRGVPQIEVTFDIDANGILNVTATDKSTGKANKITITNDKGRLSKEEIERMVQEAEKYKAEDEVQRERVSAKNALESYAFNMKSAVEDEGLKGKISEADKKKVLDKCQEVISWLDANTLAEKDEFEHKRKELEQVCNPIISGLYQGAGGPGPGGFGAQGPKGGSGSGPTIEEVD 78F513118C96DE66 nup62_human Nuclear pore glycoprotein p62 NUP62 62 kDa nucleoporin Nucleoporin Nup62 protein protein human Homo sapiens Human MSGFNFGGTGAPTGGFTFGTAKTATTTPATGFSFSTSGTGGFNFGAPFQPATSTPSTGLFSLATQTPATQTTGFTFGTATLASGGTGFSLGIGASKLNLSNTAATPAMANPSGFGLGSSNLTNAISSTVTSSQGTAPTGFVFGPSTTSVAPATTSGGFSFTGGSTAQPSGFNIGSAGNSAQPTAPATLPFTPATPAATTAGATQPAAPTPTATITSTGPSLFASIATAPTSSATTGLSLCTPVTTAGAPTAGTQGFSLKAPGAASGTSTTTSTAATATATTTSSSSTTGFALNLKPLAPAGIPSNTAAAVTAPPGPGAAAGAAASSAMTYAQLESLINKWSLELEDQERHFLQQATQVNAWDRTLIENGEKITSLHREVEKVKLDQKRLDQELDFILSQQKELEDLLSPLEELVKEQSGTIYLQHADEEREKTYKLAENIDAQLKRMAQDLKDIIEHLNTSGAPADTSDPLQQICKILNAHMDSLQWIDQNSALLQRKVEEVTKVCEGRRKEQERSFRITFD DIP protein P37198 original sequence version: 80 DIP protein P37198 original sequence version: 159 1FF65018452719A3 c11orf73-hspa1a 1 6 unspecified role unspecified role prey prey human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 4 unspecified role unspecified role bait bait ecoli Escherichia coli (strain K12) 5 unspecified role unspecified role prey prey human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 association association Supp. Fig. 2A ATP. nup62-c11orf73 2 7 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 4 unspecified role unspecified role prey prey c-terminal gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range direct interaction direct interaction Fig. 5C The interaction between Hikeshi and Nup 62 was competitively inhibited by the addition of free Nup62. nup153-c11orf73 2 4 unspecified role unspecified role prey prey region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 3 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain direct interaction direct interaction Fig. 5C The interaction between Hikeshi and FG-Nups was competitively inhibited by the addition of importin-beta complex.