MINT MINT, Dpt of Biology, University of Rome Tor Vergata MINT http://mint.bio.uniroma2.it/mint url>http://mint.bio.uniroma2.it/mint/ https://mint.bio.uniroma2.it MINT-[0-9]+ https://mint.bio.uniroma2.it/index.php/results-interactions/?id=${ac} The Molecular INTeraction database (MINT) is a relational database designed to store interactions between biological molecules. http://mint.bio.uniroma2.it Distinct requirement for an intact dimer interface in wild-type, V600E and kinase-dead B-Raf signalling. Distinct requirement for an intact dimer interface in wild-type, V600E and kinase-dead B-Raf signalling. EMBO J. (0261-4189) 2012 imex curation Röring M., Herr R., Fiala GJ., Heilmann K., Braun S., Eisenhardt AE., Halbach S., Capper D., von Deimling A., Schamel WW., Saunders DN., Brummer T. Only protein-protein interactions human-plat-e Homo sapiens plat-e plat-e anti tag coip anti tag coimmunoprecipitation anti tag coip predetermined predetermined participant predetermined Röring M., Herr R., Fiala GJ., Heilmann K., Braun S., Eisenhardt AE., Halbach S., Capper D., von Deimling A., Schamel WW., Saunders DN., Brummer T. EMBO J. (0261-4189) 2012 Only protein-protein interactions imex curation Distinct requirement for an intact dimer interface in wild-type, V600E and kinase-dead B-Raf signalling. Distinct requirement for an intact dimer interface in wild-type, V600E and kinase-dead B-Raf signalling. EMBO J. (0261-4189) 2012 imex curation Röring M., Herr R., Fiala GJ., Heilmann K., Braun S., Eisenhardt AE., Halbach S., Capper D., von Deimling A., Schamel WW., Saunders DN., Brummer T. Only protein-protein interactions mouse-embryonic_fibroblast Mus musculus embryonic fibroblasts embryonic_fibroblast Embryonic fibroblast Fibroblasts taken from an embryonic as opposed to a foetal or adult source. Foetus is defined as the stage at which tissue diferentiation is initiated. Tissue taken from an embryonic as opposed to a foetal or adult source. Embryo is defined as the development stage prior to organ formation. anti tag coip anti tag coimmunoprecipitation anti tag coip predetermined predetermined participant predetermined Röring M., Herr R., Fiala GJ., Heilmann K., Braun S., Eisenhardt AE., Halbach S., Capper D., von Deimling A., Schamel WW., Saunders DN., Brummer T. EMBO J. (0261-4189) 2012 Only protein-protein interactions imex curation Distinct requirement for an intact dimer interface in wild-type, V600E and kinase-dead B-Raf signalling. Distinct requirement for an intact dimer interface in wild-type, V600E and kinase-dead B-Raf signalling. EMBO J. (0261-4189) 2012 imex curation Röring M., Herr R., Fiala GJ., Heilmann K., Braun S., Eisenhardt AE., Halbach S., Capper D., von Deimling A., Schamel WW., Saunders DN., Brummer T. Only protein-protein interactions human-hct116 Human colon carcinoma HCT116 cells hct116 Human colon carcinoma line HCT116 anti tag coip anti tag coimmunoprecipitation anti tag coip predetermined predetermined participant predetermined Röring M., Herr R., Fiala GJ., Heilmann K., Braun S., Eisenhardt AE., Halbach S., Capper D., von Deimling A., Schamel WW., Saunders DN., Brummer T. EMBO J. (0261-4189) 2012 Only protein-protein interactions imex curation braf_human Serine/threonine-protein kinase B-raf BRAF BRAF1 RAFB1 v-Raf murine sarcoma viral oncogene homolog B1 p94 Proto-oncogene B-Raf protein protein human Homo sapiens Human MAALSGGGGGGAEPGQALFNGDMEPEAGAGAGAAASSAADPAIPEEVWNIKQMIKLTQEHIEALLDKFGGEHNPPSIYLEAYEEYTSKLDALQQREQQLLESLGNGTDFSVSSSASMDTVTSSSSSSLSVLPSSLSVFQNPTDVARSNPKSPQKPIVRVFLPNKQRTVVPARCGVTVRDSLKKALMMRGLIPECCAVYRIQDGEKKPIGWDTDISWLTGEELHVEVLENVPLTTHNFVRKTFFTLAFCDFCRKLLFQGFRCQTCGYKFHQRCSTEVPLMCVNYDQLDLLFVSKFFEHHPIPQEEASLAETALTSGSSPSAPASDSIGPQILTSPSPSKSIPIPQPFRPADEDHRNQFGQRDRSSSAPNVHINTIEPVNIDDLIRDQGFRGDGGSTTGLSATPPASLPGSLTNVKALQKSPGPQRERKSSSSSEDRNRMKTLGRRDSSDDWEIPDGQITVGQRIGSGSFGTVYKGKWHGDVAVKMLNVTAPTPQQLQAFKNEVGVLRKTRHVNILLFMGYSTKPQLAIVTQWCEGSSLYHHLHIIETKFEMIKLIDIARQTAQGMDYLHAKSIIHRDLKSNNIFLHEDLTVKIGDFGLATVKSRWSGSHQFEQLSGSILWMAPEVIRMQDKNPYSFQSDVYAFGIVLYELMTGQLPYSNINNRDQIIFMVGRGYLSPDLSKVRSNCPKAMKRLMAECLKKKRDERPLFPQILASIELLARSLPKIHRSASEPSLNRAGFQTEDFSLYACASPKTPIQAGGYGAFPVH DIP protein P15056 original sequence version: 182 DIP protein P15056 original sequence version: 133 DIP protein P15056 original sequence version: 147 DIP protein P15056 original sequence version: 173 DIP protein P15056 original sequence version: 178 DIP protein P15056 original sequence version: 188 DIP protein P15056 original sequence version: 193 DIP protein P15056 original sequence version: 195 DIP protein P15056 original sequence version: 146 DIP protein P15056 original sequence version: 153 0798C2AAB487E813 ksr1_human Kinase suppressor of Ras 1 KSR1 KSR protein protein human Homo sapiens Human MDRAALRAAAMGEKKEGGGGGDAAAAEGGAGAAASRALQQCGQLQKLIDISIGSLRGLRTKCAVSNDLTQQEIRTLEAKLVRYICKQRQCKLSVAPGERTPELNSYPRFSDWLYTFNVRPEVVQEIPRDLTLDALLEMNEAKVKETLRRCGASGDECGRLQYALTCLRKVTGLGGEHKEDSSWSSLDARRESGSGPSTDTLSAASLPWPPGSSQLGRAGNSAQGPRSISVSALPASDSPTPSFSEGLSDTCIPLHASGRLTPRALHSFITPPTTPQLRRHTKLKPPRTPPPPSRKVFQLLPSFPTLTRSKSHESQLGNRIDDVSSMRFDLSHGSPQMVRRDIGLSVTHRFSTKSWLSQVCHVCQKSMIFGVKCKHCRLKCHNKCTKEAPACRISFLPLTRLRRTESVPSDINNPVDRAAEPHFGTLPKALTKKEHPPAMNHLDSSSNPSSTTSSTPSSPAPFPTSSNPSSATTPPNPSPGQRDSRFNFPAAYFIHHRQQFIFPVPSAGHCWKCLLIAESLKENAFNISAFAHAAPLPEAADGTRLDDQPKADVLEAHEAEAEEPEAGKSEAEDDEDEVDDLPSSRRPWRGPISRKASQTSVYLQEWDIPFEQVELGEPIGQGRWGRVHRGRWHGEVAIRLLEMDGHNQDHLKLFKKEVMNYRQTRHENVVLFMGACMNPPHLAIITSFCKGRTLHSFVRDPKTSLDINKTRQIAQEIIKGMGYLHAKGIVHKDLKSKNVFYDNGKVVITDFGLFGISGVVREGRRENQLKLSHDWLCYLAPEIVREMTPGKDEDQLPFSKAADVYAFGTVWYELQARDWPLKNQAAEASIWQIGSGEGMKRVLTSVSLGKEVSEILSACWAFDLQERPSFSLLMDMLEKLPKLNRRLSHPGHFWKSADINSSKVVPRFERFGLGVLESSNPKM DIP protein Q8IVT5 original sequence version: 115 61BF0F1598349540 raf1_mouse RAF proto-oncogene serine/threonine-protein kinase Raf1 Craf Proto-oncogene c-RAF Raf-1 protein protein mouse Mus musculus Mouse MEHIQGAWKTISNGFGLKDAVFDGSSCISPTIVQQFGYQRRASDDGKLTDSSKTSNTIRVFLPNKQRTVVNVRNGMSLHDCLMKALKVRGLQPECCAVFRLLQEHKGKKARLDWNTDAASLIGEELQVDFLDHVPLTTHNFARKTFLKLAFCDICQKFLLNGFRCQTCGYKFHEHCSTKVPTMCVDWSNIRQLLLFPNSTVGDSGVPAPPSFPMRRMRESVSRMPASSQHRYSTPHAFTFNTSSPSSEGSLSQRQRSTSTPNVHMVSTTLHVDSRMIEDAIRSHSESASPSALSSSPNNLSPTGWSQPKTPVPAQRERAPGSGTQEKNKIRPRGQRDSSYYWEIEASEVMLSTRIGSGSFGTVYKGKWHGDVAVKILKVVDPTPEQLQAFRNEVAVLRKTRHVNILLFMGYMTKDNLAIVTQWCEGSSLYKHLHVQETKFQMFQLIDIARQTAQGMDYLHAKNIIHRDMKSNNIFLHEGLTVKIGDFGLATVKSRWSGSQQVEQPTGSVLWMAPEVIRMQDDNPFSFQSDVYSYGIVLYELMAGELPYAHINNRDQIIFMVGRGYASPDLSRLYKNCPKAMKRLVADCVKKVKEERPLFPQILSSIELLQHSLPKINRSASEPSLHRAAHTEDINACTLTTSPRLPVF DIP protein Q99N57 original sequence version: 97 DIP protein Q99N57 original sequence version: 132 DIP protein Q99N57 original sequence version: 92 B70104AEF51C44A5 araf_mouse Serine/threonine-protein kinase A-Raf Araf A-raf Araf1 Proto-oncogene A-Raf protein protein mouse Mus musculus Mouse MEPPRGPPVSGAEPSRAVGTVKVYLPNKQRTVVTVREGMSVYDSLDKALKVRGLNQDCCVVYRLIKGRKTVTAWDTAIAPLDGEELIVEVLEDVPLTMHNFVRKTFFSLAFCDFCLKFLFHGFRCQTCGYKFHQHCSSKVPTVCVDMSTNRRQFYHSIQDLSGGSRQQEAPSNLSVNELLTPQGPSPFTQQRDQEHFSFPAPANPPLQRIRSTSTPNVHMVSTTAPMDSSLMQFTAQSFSTDAAGRGGDGAPRGSPSPASVSSGRKSPHSKLPSEQRERKSLADEKKKVKNLGYRDSGYYWEVPPSEVQLLKRIGTGSFGTVFRGRWHGDVAVKVLKVAQPTAEQAQAFKNEMQVLRKTRHVNILLFMGFMTRPGFAIITQWCEGSSLYHHLHVADTRFDMVQLIDVARQTAQGMDYLHAKNIIHRDLKSNNIFLHEGLTVKIGDFGLATVKTRWSGAQPLEQPSGSVLWMAAEVIRMQDPNPYSFQSDVYAYGVVLYELMTGSLPYSHIGSRDQIIFMVGRGYLSPDLSKIFSNCPKAMRRLLTDCLKFQREERPLFPQILATIELLQRSLPKIERSASEPSLHRTQADELPACLLSAARLVP 05F8262F99DDD087 ksr1_mouse Kinase suppressor of Ras 1 Ksr1 Ksr Protein Hb protein protein mouse Mus musculus Mouse MDRAALRAAAMGEKKEGGGGGAAADGGAGAAVSRALQQCGQLQKLIDISIGSLRGLRTKCSVSNDLTQQEIRTLEAKLVKYICKQQQSKLSVTPSDRTAELNSYPRFSDWLYIFNVRPEVVQEIPQELTLDALLEMDEAKAKEMLRRWGASTEECSRLQQALTCLRKVTGLGGEHKMDSGWSSTDARDSSLGPPMDMLSSLGRAGASTQGPRSISVSALPASDSPVPGLSEGLSDSCIPLHTSGRLTPRALHSFITPPTTPQLRRHAKLKPPRTPPPPSRKVFQLLPSFPTLTRSKSHESQLGNRIDDVTPMKFELPHGSPQLVRRDIGLSVTHRFSTKSWLSQVCNVCQKSMIFGVKCKHCRLKCHNKCTKEAPACRITFLPLARLRRTESVPSDINNPVDRAAEPHFGTLPKALTKKEHPPAMNLDSSSNPSSTTSSTPSSPAPFLTSSNPSSATTPPNPSPGQRDSRFSFPDISACSQAAPLSSTADSTRLDDQPKTDVLGVHEAEAEEPEAGKSEAEDDEEDEVDDLPSSRRPWRGPISRKASQTSVYLQEWDIPFEQVELGEPIGQGRWGRVHRGRWHGEVAIRLLEMDGHNQDHLKLFKKEVMNYRQTRHENVVLFMGACMNPPHLAIITSFCKGRTLHSFVRDPKTSLDINKTRQIAQEIIKGMGYLHAKGIVHKDLKSKNVFYDNGKVVITDFGLFGISGVVREERRENQLKLSHDWLCYLAPEIVREMIPGRDEDQLPFSKAADVYAFGTVWYELQARDWPFKHQPAEALIWQIGSGEGVRRVLASVSLGKEVGEILSACWAFDLQERPSFSLLMDMLERLPKLNRRLSHPGHFWKSADINSSKVMPRFERFGLGTLESGNPKM EAEEB23FAE715D94 ksr2_human Kinase suppressor of Ras 2 KSR2 protein protein human Homo sapiens Human MDEENMTKSEEQQPLSLQKALQQCELVQNMIDLSISNLEGLRTKCATSNDLTQKEIRTLESKLVKYFSRQLSCKKKVALQERNAELDGFPQLRHWFRIVDVRKEVLEEISPGQLSLEDLLEMTDEQVCETVEKYGANREECARLNASLSCLRNVHMSGGNLSKQDWTIQWPTTETGKENNPVCPPEPTPWIRTHLSQSPRVPSKCVQHYCHTSPTPGAPVYTHVDRLTVDAYPGLCPPPPLESGHRSLPPSPRQRHAVRTPPRTPNIVTTVTPPGTPPMRKKNKLKPPGTPPPSSRKLIHLIPGFTALHRSKSHEFQLGHRVDEAHTPKAKKKSKPLNLKIHSSVGSCENIPSQQRSPLLSERSLRSFFVGHAPFLPSTPPVHTEANFSANTLSVPRWSPQIPRRDLGNSIKHRFSTKYWMSQTCTVCGKGMLFGLKCKNCKLKCHNKCTKEAPPCHLLIIHRGDPARLVRTESVPCDINNPLRKPPRYSDLHISQTLPKTNKINKDHIPVPYQPDSSSNPSSTTSSTPSSPAPPLPPSATPPSPLHPSPQCTRQQKNFNLPASHYYKYKQQFIFPDVVPVPETPTRAPQVILHPVTSNPILEGNPLLQIEVEPTSENEEVHDEAEESEDDFEEMNLSLLSARSFPRKASQTSIFLQEWDIPFEQLEIGELIGKGRFGQVYHGRWHGEVAIRLIDIERDNEDQLKAFKREVMAYRQTRHENVVLFMGACMSPPHLAIITSLCKGRTLYSVVRDAKIVLDVNKTRQIAQEIVKGMGYLHAKGILHKDLKSKNVFYDNGKVVITDFGLFSISGVLQAGRREDKLRIQNGWLCHLAPEIIRQLSPDTEEDKLPFSKHSDVFALGTIWYELHAREWPFKTQPAEAIIWQMGTGMKPNLSQIGMGKEISDILLFCWAFEQEERPTFTKLMDMLEKLPKRNRRLSHPGHFWKSAEL DFBC20F4E71077AD raf1_human RAF proto-oncogene serine/threonine-protein kinase RAF1 RAF Proto-oncogene c-RAF Raf-1 protein protein human Homo sapiens Human MEHIQGAWKTISNGFGFKDAVFDGSSCISPTIVQQFGYQRRASDDGKLTDPSKTSNTIRVFLPNKQRTVVNVRNGMSLHDCLMKALKVRGLQPECCAVFRLLHEHKGKKARLDWNTDAASLIGEELQVDFLDHVPLTTHNFARKTFLKLAFCDICQKFLLNGFRCQTCGYKFHEHCSTKVPTMCVDWSNIRQLLLFPNSTIGDSGVPALPSLTMRRMRESVSRMPVSSQHRYSTPHAFTFNTSSPSSEGSLSQRQRSTSTPNVHMVSTTLPVDSRMIEDAIRSHSESASPSALSSSPNNLSPTGWSQPKTPVPAQRERAPVSGTQEKNKIRPRGQRDSSYYWEIEASEVMLSTRIGSGSFGTVYKGKWHGDVAVKILKVVDPTPEQFQAFRNEVAVLRKTRHVNILLFMGYMTKDNLAIVTQWCEGSSLYKHLHVQETKFQMFQLIDIARQTAQGMDYLHAKNIIHRDMKSNNIFLHEGLTVKIGDFGLATVKSRWSGSQQVEQPTGSVLWMAPEVIRMQDNNPFSFQSDVYSYGIVLYELMTGELPYSHINNRDQIIFMVGRGYASPDLSKLYKNCPKAMKRLVADCVKKVKEERPLFPQILSSIELLQHSLPKINRSASEPSLHRAAHTEDINACTLTTSPRLPVF DIP protein P04049 original sequence version: 117 DIP protein P04049 original sequence version: 106 DIP protein P04049 original sequence version: 114 DIP protein P04049 original sequence version: 141 DIP protein P04049 original sequence version: 142 DIP protein P04049 original sequence version: 147 DIP protein P04049-1 original sequence version: 136 DIP protein P04049 original sequence version: 136 DIP protein P04049 original sequence version: 153 DIP protein P04049 original sequence version: 150 DIP protein P04049 original sequence version: 164 DIP protein P04049 original sequence version: 172 DIP protein P04049-1 original sequence version: 172 DIP protein P04049 original sequence version: 184 DIP protein P04049 original sequence version: 188 DIP protein P04049 original sequence version: 195 EF821B5349711BC3 braf-braf-23 1 B-Raf 4 anti tag western anti tag western blot anti tag western predetermined predetermined participant predetermined unspecified role unspecified role prey prey over-expressed over expressed level over-expressed living cell living cell P15056:p.Val600Glu mutation increasing interaction mutation increasing mutation increasing interaction mutation increasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain V E Tue Jul 12 10:18:47 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'mutation increasing' Wed May 02 19:58:48 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'val600glu' Tue Jun 30 19:30:48 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Val600Glu' ha tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined B-Raf 4 anti tag western anti tag western blot anti tag western predetermined predetermined participant predetermined unspecified role unspecified role bait bait over-expressed over expressed level over-expressed living cell living cell tandem tag tandem tag tandem tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined P15056:p.Val600Glu mutation increasing interaction mutation increasing mutation increasing interaction mutation increasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain V E Tue Jul 12 10:18:52 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'mutation increasing' Wed May 02 20:04:06 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'val600glu' Tue Jun 30 16:44:11 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Val600Glu' physical association physical association F3B homomint mint braf-braf-24 1 B-Raf 4 anti tag western anti tag western blot anti tag western predetermined predetermined participant predetermined unspecified role unspecified role bait bait over-expressed over expressed level over-expressed living cell living cell P15056:p.Gly469Ala mutation increasing interaction mutation increasing mutation increasing interaction mutation increasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain G A Tue Jul 12 10:18:12 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'mutation increasing' Wed May 02 18:25:58 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gly469ala' Tue Jun 30 16:37:12 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly469Ala' tandem tag tandem tag tandem tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined B-Raf 4 anti tag western anti tag western blot anti tag western predetermined predetermined participant predetermined unspecified role unspecified role prey prey over-expressed over expressed level over-expressed living cell living cell P15056:p.Gly469Ala mutation increasing interaction mutation increasing mutation increasing interaction mutation increasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain G A Tue Jul 12 10:19:38 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'mutation increasing' Wed May 02 18:47:20 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gly469ala' Tue Jun 30 15:54:45 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly469Ala' ha tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association F3c homomint mint braf-braf-25 1 B-Raf 4 anti tag western anti tag western blot anti tag western predetermined predetermined participant predetermined unspecified role unspecified role bait bait over-expressed over expressed level over-expressed living cell living cell tandem tag tandem tag tandem tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined P15056:p.Gly469Ala mutation decreasing interaction mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain G A Tue Jul 12 10:16:40 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'mutation decreasing' Wed May 02 16:57:19 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gly469ala' Tue Jun 30 19:44:05 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly469Ala' B-Raf 4 anti tag western anti tag western blot anti tag western predetermined predetermined participant predetermined unspecified role unspecified role prey prey over-expressed over expressed level over-expressed living cell living cell ha tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined P15056:p.[Gly469Ala;Arg509His] mutation increasing interaction mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain G A certain certain sequence position certain certain certain sequence position certain R H Tue Jul 12 10:18:24 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'mutation increasing' Wed May 02 14:33:42 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gly469ala' Tue Jun 30 16:55:19 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Gly469Ala;Arg509His]' physical association physical association F3c homomint mint braf-braf-26 1 B-Raf 4 anti tag western anti tag western blot anti tag western predetermined predetermined participant predetermined unspecified role unspecified role prey prey over-expressed over expressed level over-expressed living cell living cell P15056:p.Arg509His mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain R H Tue Jul 12 10:16:46 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'mutation decreasing' Wed May 02 16:33:21 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg509his' Tue Jun 30 18:14:49 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg509His' ha tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined B-Raf 4 anti tag western anti tag western blot anti tag western predetermined predetermined participant predetermined unspecified role unspecified role bait bait over-expressed over expressed level over-expressed living cell living cell tandem tag tandem tag tandem tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association homomint mint F3c f3b braf-braf-27 1 B-Raf 4 anti tag western anti tag western blot anti tag western predetermined predetermined participant predetermined unspecified role unspecified role prey prey over-expressed over expressed level over-expressed living cell living cell ha tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined P15056:p.[Arg509His;Val600Glu] mutation decreasing interaction mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain R H certain certain sequence position certain certain certain sequence position certain V E Tue Jul 12 10:16:31 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'mutation decreasing' Wed May 02 17:45:11 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg509his' Tue Jun 30 19:59:01 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Arg509His;Val600Glu]' B-Raf 4 anti tag western anti tag western blot anti tag western predetermined predetermined participant predetermined unspecified role unspecified role bait bait over-expressed over expressed level over-expressed living cell living cell tandem tag tandem tag tandem tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined P15056:p.Val600Glu mutation increasing interaction mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain V E Tue Jul 12 10:16:28 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'mutation increasing' Wed May 02 16:47:17 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'val600glu' Tue Jun 30 17:28:14 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Val600Glu' physical association physical association F3B homomint mint braf-braf-28 1 B-Raf 4 anti tag western anti tag western blot anti tag western predetermined predetermined participant predetermined unspecified role unspecified role bait bait over-expressed over expressed level over-expressed living cell living cell P15056:p.Val600Glu mutation increasing interaction mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain V E Tue Jul 12 10:16:04 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'mutation increasing' Wed May 02 15:29:34 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'val600glu' Tue Jun 30 16:53:52 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Val600Glu' tandem tag tandem tag tandem tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined B-Raf 4 anti tag western anti tag western blot anti tag western predetermined predetermined participant predetermined unspecified role unspecified role prey prey over-expressed over expressed level over-expressed living cell living cell P15056:p.Met517Trp mutation decreasing interaction mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain M W Tue Jul 12 10:54:27 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'mutation decreasing' Wed May 02 17:24:51 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'met517trp' Tue Jun 30 17:09:47 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Met517Trp' P15056:p.Leu515Gly mutation increasing interaction mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain L G Tue Jul 12 10:15:59 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'mutation increasing' Wed May 02 15:19:46 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu515gly' Tue Jun 30 17:36:31 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu515Gly' P15056:p.[Arg509His;Leu515Gly;Met517Trp] 3x mutation mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain R H certain certain sequence position certain certain certain sequence position certain L G certain certain sequence position certain certain certain sequence position certain M W Tue Jul 12 10:54:29 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'mutation decreasing' Wed May 02 16:36:09 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg509his' Tue Sep 04 17:20:24 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Arg509His;Met515Gly;Met517Trp]' Tue Jun 30 18:22:28 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Arg509His;Leu515Gly;Met517Trp]' ha tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association F3B homomint mint raf1-raf1-2 1 Raf1 10 western blot western blot Immuno blot Immuno blot predetermined predetermined participant predetermined unspecified role unspecified role bait bait over-expressed over expressed level over-expressed living cell living cell myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Raf1 10 anti tag western anti tag western blot anti tag western predetermined predetermined participant predetermined unspecified role unspecified role prey prey over-expressed over expressed level over-expressed living cell living cell ha tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association f6e homomint mint ksr1-braf 1 KSR1 8 anti tag western anti tag western blot anti tag western predetermined predetermined participant predetermined unspecified role unspecified role bait bait over-expressed over expressed level over-expressed living cell living cell myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined B-Raf 4 anti tag western anti tag western blot anti tag western predetermined predetermined participant predetermined unspecified role unspecified role prey prey over-expressed over expressed level over-expressed living cell living cell ha tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined P15056:p.Arg509His mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain R H Tue Jul 12 10:23:20 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'mutation decreasing' Wed May 02 20:05:36 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg509his' Tue Jun 30 19:10:48 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg509His' physical association physical association homomint mint sf4a First, we co-expressed HA B-Raf and mutants thereof with N-terminally Myc-tagged murine KSR1 (mKSR1), human KSR1 (hKSR1) and human KSR2 (hKSR2). Surprisingly, all HA B-Raf proteins interacted with the myc-tagged KSR proteins (Supplementary Figure S4A and B). ksr1-braf-1 1 KSR1 5 anti tag western anti tag western blot anti tag western predetermined predetermined participant predetermined unspecified role unspecified role bait bait over-expressed over expressed level over-expressed living cell living cell myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined B-Raf 4 anti tag western anti tag western blot anti tag western predetermined predetermined participant predetermined unspecified role unspecified role prey prey over-expressed over expressed level over-expressed living cell living cell ha tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association homomint mint sf4b First, we co-expressed HA B-Raf and mutants thereof with N-terminally Myc-tagged murine KSR1 (mKSR1), human KSR1 (hKSR1) and human KSR2 (hKSR2). Surprisingly, all HA B-Raf proteins interacted with the myc-tagged KSR proteins (Supplementary Figure S4A and B). ksr2-braf 1 KSR2 9 anti tag western anti tag western blot anti tag western predetermined predetermined participant predetermined unspecified role unspecified role bait bait over-expressed over expressed level over-expressed living cell living cell myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined B-Raf 4 anti tag western anti tag western blot anti tag western predetermined predetermined participant predetermined unspecified role unspecified role prey prey over-expressed over expressed level over-expressed living cell living cell ha tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association homomint mint sf4b First, we co-expressed HA B-Raf and mutants thereof with N-terminally Myc-tagged murine KSR1 (mKSR1), human KSR1 (hKSR1) and human KSR2 (hKSR2). Surprisingly, all HA B-Raf proteins interacted with the myc-tagged KSR proteins (Supplementary Figure S4A and B). raf1-braf-14 1 B-Raf 4 anti tag western anti tag western blot anti tag western predetermined predetermined participant predetermined unspecified role unspecified role prey prey over-expressed over expressed level over-expressed living cell living cell ha tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Raf1 10 anti tag western anti tag western blot anti tag western predetermined predetermined participant predetermined unspecified role unspecified role bait bait over-expressed over expressed level over-expressed living cell living cell myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association homomint mint sf4c braf-araf 2 B-Raf 4 anti tag western anti tag western blot anti tag western predetermined predetermined participant predetermined unspecified role unspecified role bait bait over-expressed over expressed level over-expressed living cell living cell ha tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Raf1 6 western blot western blot Immuno blot Immuno blot predetermined predetermined participant predetermined unspecified role unspecified role prey prey living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level A-Raf 7 western blot western blot Immuno blot Immuno blot predetermined predetermined participant predetermined unspecified role unspecified role prey prey living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level Ksr 8 western blot western blot Immuno blot Immuno blot predetermined predetermined participant predetermined unspecified role unspecified role prey prey living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level association association F7b homomint mint braf-araf-1 2 B-Raf 4 anti tag western anti tag western blot anti tag western predetermined predetermined participant predetermined unspecified role unspecified role bait bait over-expressed over expressed level over-expressed living cell living cell ha tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined P15056:p.Arg509His mutation disrupting interaction mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain R H Tue Jul 12 10:21:01 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'mutation disrupting' Wed May 02 18:50:42 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg509his' Tue Jun 30 19:52:52 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg509His' P15056:p.Met517Trp mutation disrupting interaction mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain M W Tue Jul 12 10:20:57 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'mutation disrupting' Wed May 02 16:56:04 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'met517trp' Tue Jun 30 18:26:29 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Met517Trp' P15056:p.Leu515Gly mutation disrupting interaction mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain L G Tue Jul 12 10:21:08 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'mutation disrupting' Wed May 02 17:44:01 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu515gly' Tue Jun 30 16:04:14 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu515Gly' A-Raf 7 western blot western blot Immuno blot Immuno blot predetermined predetermined participant predetermined unspecified role unspecified role prey prey living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level KSR 8 western blot western blot Immuno blot Immuno blot predetermined predetermined participant predetermined unspecified role unspecified role prey prey living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level Raf1 6 western blot western blot Immuno blot Immuno blot predetermined predetermined participant predetermined unspecified role unspecified role prey prey living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level association association homomint mint F7b sf5b In agreement with Heidorn et al (2010), but in contrast to others (Hatzivassiliou et al, 2010; Poulikakos et al, 2010), we also observe an increase in B-Raf/Raf-1 heterodimers in PLX4720-treated MEFs, although the degree of heterodimerisation is by far not as pronounced as with sorafenib or L779450 (Figure 7B). This observation can be explained by recent findings showing that binding of PLX4720 as well as PLX4032 causes a reorientation of the Ca helix (Tsai et al, 2008; Bollag et al, 2010), which in turn could affect DIF function. This drug-induced reorientation might also explain the absence of B-Raf/A-Raf and B-Raf/ KSR1 heterodimers. In contrast, Raf-1 was still present in B-RafR509H immunecomplexes from PLX4720-treated cells. Interestingly, while the R509H substitution has only a minor effect on sorafenib-induced B-Raf/Raf-1 heterodimerisation, the same mutation abrogates the prominent formation of stable L779450-induced B-Raf/Raf-1 and B-Raf/A-Raf complexes (Figure 7B). Furthermore, the drug-induced formation of B-Raf/KSR1 complexes was also prevented by the R509H mutation as it was recently reported (McKay et al, 2011). we next asked whether an intact DIF is required for drug-induced heterodimerisation (Supplementary Figure S5A). To this end, we used sorafenib that inhibits several kinases including all Raf isoforms and therefore also ERK pathway activation (Figure 7A; Wilhelm et al, 2004; Heidorn et al, 2010). As a result, B-Raf/Raf-1 heterodimers, which are disrupted by ERK-mediated feedback phosphorylation (Rushworth et al, 2006; Heidorn et al, 2010; Ritt et al, 2010), are preserved and particularly abundant even in the absence of 4-HT triggered ERTmRASV12 signalling (Figure 7B; Supplementary Figure S5B). Importantly, this experiment revealed for the first time distinct structural requirements within the DIF for the formation of sorafenib-induced B-Raf containing heterodimers. We observed low levels of heterodimers between Raf-1 and A-Raf with B-Raf including its DIF mutants in vehicle (DMSO)-treated cells with active ERTmRASV12 signalling. Upon sorafenib treatment, however, the R509H mutation in B-Raf prevents the strong drug-in-duced increase in A-Raf and KSR1 containing heterodimers, while the B-Raf/Raf-1 interaction is less affected (Figure 7B; Supplementary Figure S5B). Nevertheless, the 3x mutation prevents sorafenib-induced B-Raf/Raf-1 heterodimerisation indicating an important contribution of L515 and M517 in this process. braf-raf1-26 2 B-Raf 4 anti tag western anti tag western blot anti tag western predetermined predetermined participant predetermined unspecified role unspecified role bait bait over-expressed over expressed level over-expressed living cell living cell P15056:p.Asp594Ala mutation disrupting interaction mutation increasing mutation increasing interaction mutation increasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain D A Tue Jul 12 10:21:11 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'mutation disrupting' Wed May 02 20:14:00 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asp594ala' Tue Jun 30 16:59:17 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asp594Ala' ha tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Raf1 6 western blot western blot Immuno blot Immuno blot predetermined predetermined participant predetermined unspecified role unspecified role prey prey living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level physical association physical association f6d homomint mint braf-raf1-27 3 Raf1 10 western blot western blot Immuno blot Immuno blot predetermined predetermined participant predetermined unspecified role unspecified role prey prey living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level B-Raf 4 anti tag western anti tag western blot anti tag western predetermined predetermined participant predetermined unspecified role unspecified role bait bait over-expressed over expressed level over-expressed living cell living cell P15056:p.Met517Trp mutation disrupting interaction mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain M W Tue Jul 12 10:20:52 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'mutation disrupting' Wed May 02 17:15:18 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'met517trp' Tue Jun 30 20:12:54 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Met517Trp' P15056:p.Leu515Gly mutation disrupting interaction mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain L G Tue Jul 12 10:20:53 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'mutation disrupting' Wed May 02 19:39:28 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu515gly' Tue Jun 30 18:10:10 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu515Gly' P15056:p.Arg509His mutation disrupting interaction mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain R H Tue Jul 12 10:08:22 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'mutation disrupting' Wed May 02 15:31:55 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg509his' Tue Jun 30 17:06:31 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg509His' ha tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association homomint mint sf6a sf6b Again, as we have observed in our MEF system, the sorafenib-induced increase of the B-Raf/Raf-1 interaction was completely blunted by the 3x, but not the R509H mutation (Supplementary Figure S6A). Very similar findings were obtained using an ecoR subline of the human colon carcinoma cell line HCT116 (Supplementary Figure S6B), which is driven by a KRASG13D oncogene (Shirasawa et al, 1993). For an additional comparison, we also analysed the heterodimerisation behaviour of the Ras binding-deficient mutant B-RafR188L, which failed to interact with Raf-1 in both colon carcinoma cell lines as it has already been shown by Heidorn et al (2010) for melanoma cell lines. braf-raf1-28 3 Raf1 10 western blot western blot Immuno blot Immuno blot predetermined predetermined participant predetermined unspecified role unspecified role prey prey living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level B-Raf 4 anti tag western anti tag western blot anti tag western predetermined predetermined participant predetermined unspecified role unspecified role bait bait over-expressed over expressed level over-expressed living cell living cell P15056:p.Arg509His mutation disrupting interaction mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain R H Tue Jul 12 10:07:14 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'mutation disrupting' Wed May 02 18:22:43 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg509his' Tue Jun 30 16:48:49 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg509His' ha tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association homomint mint sf6a sf6b Again, as we have observed in our MEF system, the sorafenib-induced increase of the B-Raf/Raf-1 interaction was completely blunted by the 3x, but not the R509H mutation (Supplementary Figure S6A). Very similar findings were obtained using an ecoR subline of the human colon carcinoma cell line HCT116 (Supplementary Figure S6B), which is driven by a KRASG13D oncogene (Shirasawa et al, 1993). For an additional comparison, we also analysed the heterodimerisation behaviour of the Ras binding-deficient mutant B-RafR188L, which failed to interact with Raf-1 in both colon carcinoma cell lines as it has already been shown by Heidorn et al (2010) for melanoma cell lines. braf-raf1-29 3 B-Raf 4 anti tag western anti tag western blot anti tag western predetermined predetermined participant predetermined unspecified role unspecified role bait bait over-expressed over expressed level over-expressed living cell living cell ha tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined P15056:p.Asp594Ala mutation disrupting interaction mutation increasing mutation increasing interaction mutation increasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain D A Tue Jul 12 10:50:20 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'mutation disrupting' Wed May 02 18:54:16 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asp594ala' Tue Jun 30 19:45:48 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asp594Ala' Raf1 10 western blot western blot Immuno blot Immuno blot predetermined predetermined participant predetermined unspecified role unspecified role prey prey living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level physical association physical association homomint mint sf6a sf6b Again, as we have observed in our MEF system, the sorafenib-induced increase of the B-Raf/Raf-1 interaction was completely blunted by the 3x, but not the R509H mutation (Supplementary Figure S6A). Very similar findings were obtained using an ecoR subline of the human colon carcinoma cell line HCT116 (Supplementary Figure S6B), which is driven by a KRASG13D oncogene (Shirasawa et al, 1993). For an additional comparison, we also analysed the heterodimerisation behaviour of the Ras binding-deficient mutant B-RafR188L, which failed to interact with Raf-1 in both colon carcinoma cell lines as it has already been shown by Heidorn et al (2010) for melanoma cell lines. braf-raf1-30 3 Raf1 10 western blot western blot Immuno blot Immuno blot predetermined predetermined participant predetermined unspecified role unspecified role prey prey living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level B-Raf 4 anti tag western anti tag western blot anti tag western predetermined predetermined participant predetermined unspecified role unspecified role bait bait over-expressed over expressed level over-expressed living cell living cell ha tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined P15056:p.Arg188Leu mutation disrupting interaction mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain R L Tue Jul 12 10:08:02 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'mutation disrupting' Wed May 02 14:26:43 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg188leu' Tue Jun 30 15:52:57 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg188Leu' physical association physical association homomint mint sf6a sf6b Again, as we have observed in our MEF system, the sorafenib-induced increase of the B-Raf/Raf-1 interaction was completely blunted by the 3x, but not the R509H mutation (Supplementary Figure S6A). Very similar findings were obtained using an ecoR subline of the human colon carcinoma cell line HCT116 (Supplementary Figure S6B), which is driven by a KRASG13D oncogene (Shirasawa et al, 1993). For an additional comparison, we also analysed the heterodimerisation behaviour of the Ras binding-deficient mutant B-RafR188L, which failed to interact with Raf-1 in both colon carcinoma cell lines as it has already been shown by Heidorn et al (2010) for melanoma cell lines.