IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Biochemically distinct vesicles from the endoplasmic reticulum fuse to form peroxisomes. Biochemically distinct vesicles from the endoplasmic reticulum fuse to form peroxisomes. Cell (0092-8674) 2012 imex curation van der Zand A., Gent J., Braakman I., Tabak H.F. Experiments performed in wild-type cells, which have a peroxisome. a.vanderzand@uu.nl 20-Jun-2012: Contacted by JYOTI. Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast bifc bimolecular fluorescence complementation bifc tag fluorescence tag visualisation by fluorescence tag fluorescence van der Zand A., Gent J., Braakman I., Tabak H.F. Experiments performed in wild-type cells, which have a peroxisome. a.vanderzand@uu.nl Accepted 2012-JUN-08 by PPORRAS Cell (0092-8674) 2012 Only protein-protein interactions imex curation Biochemically distinct vesicles from the endoplasmic reticulum fuse to form peroxisomes. Biochemically distinct vesicles from the endoplasmic reticulum fuse to form peroxisomes. Cell (0092-8674) 2012 imex curation van der Zand A., Gent J., Braakman I., Tabak H.F. Experiments performed in wild-type cells, which have a peroxisome. a.vanderzand@uu.nl 20-Jun-2012: Contacted by JYOTI. Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast bifc bimolecular fluorescence complementation bifc tag fluorescence tag visualisation by fluorescence tag fluorescence van der Zand A., Gent J., Braakman I., Tabak H.F. Experiments performed in wild-type cells, which have a peroxisome. a.vanderzand@uu.nl Accepted 2012-JUN-08 by PPORRAS 20-Jun-2012: Contacted by JYOTI. Cell (0092-8674) 2012 Only protein-protein interactions imex curation Biochemically distinct vesicles from the endoplasmic reticulum fuse to form peroxisomes. Biochemically distinct vesicles from the endoplasmic reticulum fuse to form peroxisomes. Cell (0092-8674) 2012 imex curation van der Zand A., Gent J., Braakman I., Tabak H.F. Experiments performed in wild-type cells, which have a peroxisome. a.vanderzand@uu.nl 20-Jun-2012: Contacted by JYOTI. Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag immunost anti tag immunostaining anti tag immunost van der Zand A., Gent J., Braakman I., Tabak H.F. Experiments performed in wild-type cells, which have a peroxisome. a.vanderzand@uu.nl Accepted 2012-JUN-08 by PPORRAS 20-Jun-2012: Contacted by JYOTI. Cell (0092-8674) 2012 Only protein-protein interactions imex curation Biochemically distinct vesicles from the endoplasmic reticulum fuse to form peroxisomes. Biochemically distinct vesicles from the endoplasmic reticulum fuse to form peroxisomes. Cell (0092-8674) 2012 imex curation van der Zand A., Gent J., Braakman I., Tabak H.F. Experiments performed in wild-type cells, which have a peroxisome. a.vanderzand@uu.nl 20-Jun-2012: Contacted by JYOTI. Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast bifc bimolecular fluorescence complementation bifc tag fluorescence tag visualisation by fluorescence tag fluorescence van der Zand A., Gent J., Braakman I., Tabak H.F. Experiments performed in wild-type cells, which have a peroxisome. a.vanderzand@uu.nl Accepted 2012-JUN-08 by PPORRAS 20-Jun-2012: Contacted by JYOTI. Cell (0092-8674) 2012 Only protein-protein interactions imex curation Biochemically distinct vesicles from the endoplasmic reticulum fuse to form peroxisomes. Biochemically distinct vesicles from the endoplasmic reticulum fuse to form peroxisomes. Cell (0092-8674) 2012 imex curation van der Zand A., Gent J., Braakman I., Tabak H.F. Experiments performed in wild-type cells, which have a peroxisome. a.vanderzand@uu.nl 20-Jun-2012: Contacted by JYOTI. Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast fluorescence imaging fluorescence microscopy fluorescence imaging tag fluorescence tag visualisation by fluorescence tag fluorescence van der Zand A., Gent J., Braakman I., Tabak H.F. Experiments performed in wild-type cells, which have a peroxisome. a.vanderzand@uu.nl Accepted 2012-JUN-08 by PPORRAS 20-Jun-2012: Contacted by JYOTI. Cell (0092-8674) 2012 Only protein-protein interactions imex curation pex11_yeast Peroxisomal membrane protein PMP27 PEX11 PMP24 PMP27 O0454 YOL147C Peroxin-11 protein protein yeast Saccharomyces cerevisiae Baker's yeast MVCDTLVYHPSVTRFVKFLDGSAGREKVLRLLQYLARFLAVQNSSLLARQLQAQFTTVRKFLRFLKPLNHLQAAAKFYDNKLASDNVVRVCNVLKNIFFAAYLSLDQVNLLRILKVIPVTVLTGKKIPRWSNWCWLFGLLSGLAMDLRKIQTSHAQIAAFVKAKSQSQGDEHEDHKKVLGKAYQDRYTALRRLFWDAADSFIVLNNLGYLSSNEEYVALSGVVTSILGMQDMWKAT 26B95896D57018DD pex6_yeast Peroxisomal ATPase PEX6 PEX6 PAS8 YNL329C N0310 Peroxin-6 Peroxisome biosynthesis protein PAS8 Peroxisomal assembly protein 8 protein protein yeast Saccharomyces cerevisiae Baker's yeast MKASLTFSLSGIYAPCSISRDIYLEYGDKKAECLYGTIRLPQYGPGCTPGKIVHCVLDDSLPFCSIVVPSKLFGFMPTQPTMDFCYFEPILDNVVPVLDSVTFLINEQLYSKLMDLPQEMQQIQFLHYKYNINSMETVVHSRDILTSGLCQILNCSPFPQGLVDFTETQLILVNDTEQKLSALKYANEDEEYALPKIGTNSALSIDLESLPCTISRDLLRPAPHINDDNSIYAFTDAETLLRLDVTSGSFITVSNMGCVRLVKLFVLLLPNGFKKRTIYAPPKIIASFPDCSVVTISKSNIGHTDIPIANQVFISRVGGWLQSQKCFQNIILTTLKKFFSESKRILCQNDLIPIAFDSSMADLNIAEENDESDDEDELGQYYKNDSLVWFFVTSAELDCFSKDNSHFIIDPNRTKLITTNITNRRPLPLSRSNLQRYYGFAETFYYDLHIFPYVRQLVNILETSFNCSQRGITLNASVLLHSTTNNVGKATMVRFASKYLGIHLLEIDCLSLTSNSRQLDSTSKIIGYIRAKCENVLPYASPAVIFLAHLDSILLDVNANQDPEAIKLQKSINFEMSKLLDDFTFKFPGTTFVGSVNNIDNVPSSFRSHMRFEILVPVPSEAQRLRIFQWYLSSHELNRDVQQKVPVSYMDNISFSSLSSYSAGLTPLDIKSIVETARMTATARFYQESKKCGWLPQSILITQEDLSKATSKARNEFSVSIGAPQIPNVTWDDIGGIDFVKGEILDTIDMPLKHPELFTSGMKKRSGILFYGPPGTGKTLMAKAIATNFSLNFFSVKGPELLNMYIGESEANVRRVFQKAREAKPCVIFFDEIDSVAPKRGNQGDSGGVMDRIVSQLLAELDGMSTDADGVFVIGATNRPDLLDEALLRPGRFDKLLYLGIPDTDTKQLNILEALTRKFVLDNDVKLIELAKLCPFNYTGADFYALCSDAMLNAMSRIARMVEKKVSQHNELTGENISTRRWFDKIATKEDTKVVVKMEDFLKAQEQLTPSVSRAELNHYEAVRANFEGA 736FECB57BBFF8F8 736FECB57BBFF8F8 pex13_yeast Peroxisomal membrane protein PAS20 PEX13 PAS20 YLR191W L9470.1 Peroxin-13 protein protein yeast Saccharomyces cerevisiae Baker's yeast MSSTAVPRPKPWETSASLEEPQRNAQSLSAMMTSNQQDSRPTEESNNSNSASESAPEVLPRPAALNSSGTYGESNTIPGIYGNSNYGIPYDNNPYSMNSIYGNSIGRYGYGGSYYGNNYGSFYGGGYGAGAGYGMNNGSGLGESTKATFQLIESLIGAVTGFAQMLESTYMATHNSFFTMISVAEQFGNLKEMLGSFFGIFAIMKFLKKILYRATKGRLGIPPKNFAESEGSKNKLIEDFQKFNDSGTINSNEKATRRKISWKPLLFFLMAVFGFPYLLNKFITKLQTSGTIRASQGNGSEPIDPSKLEFARALYDFVPENPEMEVALKKGDLMAILSKKDPLGRDSDWWKVRTKNGNIGYIPYNYIEIIKRRKKIEHVDDETRTH DBEA9A2372185860 DBEA9A2372185860 pex2_yeast Peroxisomal biogenesis factor 2 PEX2 CRT1 PAS5 YJL210W J0240 HRA271 Peroxin-2 Peroxisomal protein PAS5 Protein CRT1 protein protein yeast Saccharomyces cerevisiae Baker's yeast MSRVAQLDSIALDKELYGQFWSEFNAAFNTSEHKEEWELALNTVVFMCATRFLPHYGSSCTYGSALSGVVFQCRKRTLYVVTVLAGYVWKKITHIIFNGPHCGNQMMWLKLYKWVNLLYHGCDVTNFLRFLAAEGPNARAFLSPLYRAFNVHSTRLIRDGSAIASEFYSNSVFAGLEYQNRQLLWNALLELFSNTLLTKRGLLTFVKKPPRSRSTTTYKTVCPRCGGFPTNPYQIACCRANYCYVCVVKALEWSMCDACGSSGRLTASPVY FAEFA3A526E82F3E FAEFA3A526E82F3E pex10_yeast Peroxisome biogenesis factor 10 PAS4 YDR265W D9954.8 PEX10 Peroxin-10 Peroxisome assembly protein 10 Peroxisomal biogenesis factor 10 protein protein yeast Saccharomyces cerevisiae Baker's yeast MKNDNKLQKEALMRLSQLRFPFADAPSIVQAHQKDEQIQGLLIMKVTELCKLIKSQLFVNSYPKELSIFAKLLYLLFTTGRRGRTLGEEYVDLTYTNRKGTRLAGRLKMIVFAFAYPLCPYFITKLYKKIMKNNKESKIEDTESVAAFCKGLLDFILDVHMTLFYFKGAFYSISKRIFGMRYVFKHILSKNEANFREEGSQKYKVLGYILLAQNVMKWYPVLTSTLGSWIYGRKRTNDSITRSSVGLQERSEHESIEGIPKESQLTHINLSDKNQLPFIPEASRKCILCLMNMSDPSCAPCGHLFCWSCLMSWCKERPECPLCRQHCQPQEILVLRQ 9AF308A8AB2A0E28 pex14_yeast Peroxisomal membrane protein PEX14 PEX14 YGL153W G1870 Peroxin-14 protein protein yeast Saccharomyces cerevisiae Baker's yeast MSDVVSKDRKALFDSAVSFLKDESIKDAPLLKKIEFLKSKGLTEKEIEIAMKEPKKDGIVGDEVSKKIGSTENRASQDMYLYEAMPPTLPHRDWKDYFVMATATAGLLYGAYEVTRRYVIPNILPEAKSKLEGDKKEIDDQFSKIDTVLNAIEAEQAEFRKKESETLKELSDTIAELKQALVQTTRSREKIEDEFRIVKLEVVNMQNTIDKFVSDNDGMQELNNIQKEMESLKSLMNNRMESGNAQDNRLFSISPNGIPGIDTIPSASEILAKMGMQEESDKEKENGSDANKDDNAVPAWKKAREQTIDSNASIPEWQKNTAANEISVPDWQNGQVEDSIP B714D7DA045E2DCB B714D7DA045E2DCB pex12_yeast Peroxisome assembly protein 12 PEX12 Peroxin-12 YM9711.16C YMR026C protein protein yeast Saccharomyces cerevisiae Baker's yeast MSFYSNLPSAGQSSRGSSTSGRNGVGLEPLYPTIFEIMSSQEIDSLLPASIRYLLANHLVANFPNRYTLRLNKYFFEWFQAIKGFVEWYHLKTYNSTFIDRFYGLQLFSSRDRNLALTQCLNPKGQSEWPQGLQLNQQQKSVIFLEKIILPYITAKLDEILEKISMNNIFSSDETENKWPKRAFLRIYPFIKKLLALSNLLVKLLFLTKRTGSVSLLQYLFKIEYTTVRPLSSELSGLKETKGMDNRLRKTNISSIFALMQGQLSIIPRFLTFMGSQFFPTFIFVLRVYQWWTTQDMTTKLQKRVNDLDEDIPRPPFSSHSDKTEDKEGVSEACPVCEKTVQNPCVLETGYVACYPCAISYLVNNEGHCPVTNKKLLGCTYNKHTNKWEVVTGIRKLLI 98E6300C2CBFA16A 98E6300C2CBFA16A pex1_yeast Peroxisomal ATPase PEX1 YKL197C PEX1 PAS1 Peroxin-1 Peroxisome biogenesis protein PAS1 Peroxisomal assembly protein 1 protein protein yeast Saccharomyces cerevisiae Baker's yeast MTTTKRLKFENLRIQFSNAIVGNFLRLPHSIINVLESTNYAIQEFGIAVHSHNSDIPIVHLGWDGHDSGSSENVVLINPVLATVYDLNQKSPLVDLYIQRYDHTHLATEVYVTPETSDDWEIIDANAMRFQNGEILHQTRIVTPGETLICYLEGIVTKFKIDRVEPSMKSARITDGSLVVVAPKVNKTRLVKAEYGHSNKTILKNGAIQLLKKVILRSTVCKMDFPKDNLFVVYISDGAQLPSQKGYASIVKCSLRQSKKSDSDNKSVGIPSKKIGVFIKCDSQIPENHIALSSHLWDAFFTHPMNGAKIKLEFLQMNQANIISGRNATVNIKYFGKDVPTKSGDQYSKLLGGSLLTNNLILPTEQIIIEIKKGESEQQLCNLNEISNESVQWKVTQMGKEEVKDIIERHLPKHYHVKETGEVSRTSKDEDDFITVNSIKKEMVNYLTSPIIATPAIILDGKQGIGKTRLLKELINEVEKDHHIFVKYADCETLHETSNLDKTQKLIMEWCSFCYWYGPSLIVLDNVEALFGKPQANDGDPSNNGQWDNASKLLNFFINQVTKIFNKDNKRIRVLFSGKQKTQINPLLFDKHFVSETWSLRAPDKHARAKLLEYFFSKNQIMKLNRDLQFSDLSLETEGFSPLDLEIFTEKIFYDLQLERDCDNVVTRELFSKSLSAFTPSALRGVKLTKETNIKWGDIGALANAKDVLLETLEWPTKYEPIFVNCPLRLRSGILLYGYPGCGKTLLASAVAQQCGLNFISVKGPEILNKFIGASEQNIRELFERAQSVKPCILFFDEFDSIAPKRGHDSTGVTDRVVNQLLTQMDGAEGLDGVYILAATSRPDLIDSALLRPGRLDKSVICNIPTESERLDILQAIVNSKDKDTGQKKFALEKNADLKLIAEKTAGFSGADLQGLCYNAYLKSVHRWLSAADQSEVVPGNDNIEYFSINEHGRREENRLRLKTLLQQDVVHETKTSTSAASELTAVVTINDLLEACQETKPSISTSELVKLRGIYDRFQKDRNGEMPNGENSIDIGSRLSLM EB13C080FB8189DB pex10-pex2 1 9 unspecified role unspecified role neutral component neutral component c-terminal venus-n venus n-terminal fragment c-term range C-terminal range c-term range c-term range C-terminal range c-term range 10 unspecified role unspecified role neutral component neutral component c-terminal venus-c venus c-terminal fragment c-term range C-terminal range c-term range c-term range C-terminal range c-term range physical association physical association Fig. 1B, 1C, 6B A further BIFC study confirmed the interaction occurred within the peroxisome. pex14-pex2 1 11 unspecified role unspecified role neutral component neutral component c-terminal venus-c venus c-terminal fragment c-term range C-terminal range c-term range c-term range C-terminal range c-term range 9 unspecified role unspecified role neutral component neutral component c-terminal venus-n venus n-terminal fragment c-term range C-terminal range c-term range c-term range C-terminal range c-term range physical association physical association Fig. 1B, 1C, 6B A further BIFC study confirmed the interaction occurred within the peroxisome. pex13-pex14 1 8 unspecified role unspecified role neutral component neutral component c-terminal venus-n venus n-terminal fragment c-term range C-terminal range c-term range c-term range C-terminal range c-term range 11 unspecified role unspecified role neutral component neutral component c-terminal venus-c venus c-terminal fragment c-term range C-terminal range c-term range c-term range C-terminal range c-term range physical association physical association Fig. 1B, 1C, 6B A further BIFC study confirmed the interaction occurred within the peroxisome. pex10-pex13 1 8 unspecified role unspecified role neutral component neutral component c-terminal venus-n venus n-terminal fragment c-term range C-terminal range c-term range c-term range C-terminal range c-term range 10 unspecified role unspecified role neutral component neutral component c-terminal venus-c venus c-terminal fragment c-term range C-terminal range c-term range c-term range C-terminal range c-term range physical association physical association Fig. 1B, 1C, 6B A further BIFC study confirmed the interaction occurred within the peroxisome. pex1-pex6-3 1 7 unspecified role unspecified role neutral component neutral component c-terminal venus-n venus n-terminal fragment c-term range C-terminal range c-term range c-term range C-terminal range c-term range 13 unspecified role unspecified role neutral component neutral component c-terminal venus-c venus c-terminal fragment c-term range C-terminal range c-term range c-term range C-terminal range c-term range physical association physical association Supp. Fig. 1 pex1-pex6-4 1 7 unspecified role unspecified role neutral component neutral component c-terminal venus-n venus n-terminal fragment c-term range C-terminal range c-term range c-term range C-terminal range c-term range 13 unspecified role unspecified role neutral component neutral component c-terminal venus-c venus c-terminal fragment c-term range C-terminal range c-term range c-term range C-terminal range c-term range physical association physical association Supp. Fig. 1 pex10-pex2-1 2 9 unspecified role unspecified role neutral component neutral component c-terminal venus-n venus n-terminal fragment c-term range C-terminal range c-term range c-term range C-terminal range c-term range 10 unspecified role unspecified role neutral component neutral component c-terminal venus-c venus c-terminal fragment c-term range C-terminal range c-term range c-term range C-terminal range c-term range physical association physical association Supp. Fig. 3 A further BIFC study confirmed the interaction occurred within the peroxisome. pex13-pex14-1 2 8 unspecified role unspecified role neutral component neutral component c-terminal venus-n venus n-terminal fragment c-term range C-terminal range c-term range c-term range C-terminal range c-term range 11 unspecified role unspecified role neutral component neutral component c-terminal venus-c venus c-terminal fragment c-term range C-terminal range c-term range c-term range C-terminal range c-term range physical association physical association Supp. Fig. 3 pex10-pex2-2 4 10 unspecified role unspecified role neutral component neutral component c-terminal venus-c venus c-terminal fragment c-term range C-terminal range c-term range c-term range C-terminal range c-term range 9 unspecified role unspecified role neutral component neutral component c-terminal venus-n venus n-terminal fragment c-term range C-terminal range c-term range c-term range C-terminal range c-term range physical association physical association Supp. Fig. 4 A further BIFC study confirmed the interaction occurred within the peroxisome. pex13-pex14-2 4 11 unspecified role unspecified role neutral component neutral component c-terminal venus-c venus c-terminal fragment c-term range C-terminal range c-term range c-term range C-terminal range c-term range 8 unspecified role unspecified role neutral component neutral component c-terminal venus-n venus n-terminal fragment c-term range C-terminal range c-term range c-term range C-terminal range c-term range physical association physical association Sup. Fig. 4 pex13-pex2 3 8 unspecified role unspecified role bait bait region cfp tag cyan fluorescent protein tag CFP cyan fluorescent protein cyan fluorescent protein CFP cfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 9 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Fig. 3A, Supp Fig. 6A In pex19-/- cells, where ER exit was blocked, the interaction of Pex2p with Pex13p was not observed. pex13-pex14-3 5 11 unspecified role unspecified role neutral component neutral component c-terminal yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range 8 unspecified role unspecified role neutral component neutral component c-terminal cfp tag cyan fluorescent protein tag CFP cyan fluorescent protein cyan fluorescent protein CFP cfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range colocalization colocalization Fig. 3B, Supp. Fig. 6C pex10-pex2-3 5 9 unspecified role unspecified role neutral component neutral component c-terminal yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range 10 unspecified role unspecified role neutral component neutral component c-terminal yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range colocalization colocalization Fig. 3B pex10-pex13-1 5 8 unspecified role unspecified role neutral component neutral component c-terminal cfp tag cyan fluorescent protein tag CFP cyan fluorescent protein cyan fluorescent protein CFP cfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range 10 unspecified role unspecified role neutral component neutral component c-terminal yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range colocalization colocalization Fig. 3B pex13-pex2-1 5 8 unspecified role unspecified role neutral component neutral component c-terminal cfp tag cyan fluorescent protein tag CFP cyan fluorescent protein cyan fluorescent protein CFP cfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range 9 unspecified role unspecified role neutral component neutral component c-terminal yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range colocalization colocalization Fig. 3D pex12-pex2 5 9 unspecified role unspecified role neutral component neutral component c-terminal yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range 12 unspecified role unspecified role neutral component neutral component n-terminal yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range colocalization colocalization Supp. Fig. 6C pex12-pex13 5 8 unspecified role unspecified role neutral component neutral component c-terminal yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range 12 unspecified role unspecified role neutral component neutral component n-terminal yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range colocalization colocalization Supp. Fig. 6C pex11-pex13-1 5 6 unspecified role unspecified role neutral component neutral component c-terminal yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range 8 unspecified role unspecified role neutral component neutral component c-terminal cfp tag cyan fluorescent protein tag CFP cyan fluorescent protein cyan fluorescent protein CFP cfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range colocalization colocalization Fig. 4B, 4C, Supp. Fig. 7A, 7C pex11-pex2-1 5 6 unspecified role unspecified role neutral component neutral component c-terminal yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range 9 unspecified role unspecified role neutral component neutral component c-terminal cfp tag cyan fluorescent protein tag CFP cyan fluorescent protein cyan fluorescent protein CFP cfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range colocalization colocalization Supp. Fig. 7A, 7C pex14-pex2-1 4 11 unspecified role unspecified role neutral component neutral component c-terminal venus-c venus c-terminal fragment c-term range C-terminal range c-term range c-term range C-terminal range c-term range 9 unspecified role unspecified role neutral component neutral component c-terminal venus-n venus n-terminal fragment c-term range C-terminal range c-term range c-term range C-terminal range c-term range physical association physical association Fig. 5 A further BIFC study confirmed the interaction occurred within the peroxisome. pex10-pex13-2 4 8 unspecified role unspecified role neutral component neutral component c-terminal venus-n venus n-terminal fragment c-term range C-terminal range c-term range c-term range C-terminal range c-term range 10 unspecified role unspecified role neutral component neutral component c-terminal venus-c venus c-terminal fragment c-term range C-terminal range c-term range c-term range C-terminal range c-term range physical association physical association Fig. 5