MINT MINT, Dpt of Biology, University of Rome Tor Vergata MINT http://mint.bio.uniroma2.it/mint url>http://mint.bio.uniroma2.it/mint/ https://mint.bio.uniroma2.it MINT-[0-9]+ https://mint.bio.uniroma2.it/index.php/results-interactions/?id=${ac} The Molecular INTeraction database (MINT) is a relational database designed to store interactions between biological molecules. http://mint.bio.uniroma2.it Functional characterization of UvrD helicases from Haemophilus influenzae and Helicobacter pylori. Functional characterization of UvrD helicases from Haemophilus influenzae and Helicobacter pylori. FEBS J. (1742-464X) 2012 Sharma R., Rao DN. in vitro In vitro crosslink cross-linking study crosslink weight silver stain molecular weight estimation by silver staining weight silver stain Sharma R., Rao DN. FEBS J. (1742-464X) 2012 Functional characterization of UvrD helicases from Haemophilus influenzae and Helicobacter pylori. Functional characterization of UvrD helicases from Haemophilus influenzae and Helicobacter pylori. FEBS J. (1742-464X) 2012 Sharma R., Rao DN. in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Sharma R., Rao DN. FEBS J. (1742-464X) 2012 o26013_helpy DNA helicase HP_1478 protein protein helpy Helicobacter pylori Campylobacter pylori MMDFEKSILDHLNGAQKIAASHIQGPLLILAGAGSGKTKTLTSRLAYLIGVCGVPSENTLTLTFTNKASKEMQERALKLLKNQALIPPLLCTFHRFGLLFLRQHMNLLKRACDFSVLDSDEVKTLCKQLKISNFRASISQIKNGMMDLSMQDSECYKAYELYQNALKKDNLVDFDDLLFLSLKILQDNETIAKETSERYHYIMVDEYQDTNALQLEFLKKLSFTHHNLCVVGDDDQSIYGFRGADISNILNFSKHFKGAKIVKLETNYRSSAEILACANSLISHNQHRHIKTLQSFKGSHKSVVCKEYLTQKEESLDVAYQIKALLKKGENLENIAILYRLNGLSRSIEESLNALNIPYRLIGALSFYERAEIKDALAFMHLVAKKDDRFFIKRVLNKPPRGLGKITQEWIFSLLDEEGLNLEEALKLGAFKDKLNPKNEYALKQFIAMIGRLREAFEISVEEFCSRFLEETNLLKSYEKEDNYEEREGFVKELLTLVKEYFKTNPTHSLLDFLNESVLDAHNTENAQKVSCMSVHMSKGLEFKHVFVIGLEEGFFPHRGFNQESDLEEERRLAYVAITRAKEELQLSYVKERSYFGRKISCSPSVFLEEAQLLNQDNPPKQDHQKDAPIKVGDLIRHKIFGTGRVLGVEKGLSGLCLKINCGGNVYDKISEKFVEKVDNGF mint 208383EE501493B0 uvrd_haein DNA helicase II uvrD mutB HI_1188 protein protein haein Haemophilus influenzae MMDISELLDGLNDKQRERVAAPLGNHLVLAGAGSGKTRVLTHRIAWLIAVENISEGSIMAVTFTNKAAAEMRHRIQSTLAKHAQHQLFGMWIGTFHSIAHRLLRAHHLDVGLPQDFQILDSEDQLRLIKRLLKLHNFDEKAFPPKQACWYINNKKDEGLRPNDIEDFNDRQEREWIKIYQIYQDTCDRAGLVDFAELLIRVYELFEKKPLILQRYQQRFQHILVDEFQDTNKIQYKWIKILAGKTGQVMIVGDDDQSIYGWRGAQIENIQKFLKDFKAETIRLEQNYRSTANILNSANELIANNSDRLGKNLWTEGEKGDPVGIYSAFNELDEAKFVASQIQDWVEHGGKLDDCAVLYRSNSQSRVIEEALIRCQIPYRIYGGMRFFERQEIKDALAYLRLINNRQDDAAFERVINTPTRGIGDRTLDILRNLTRERQITLWQAVQVATQENMLAGRASTALLRFQELINSLQLDTAEMPLFAQTDFVIKHSGLYEMYQQEKGEKGEVRIENLEELVTATREFIKPDNAEEMTELTAFLTHASLEAGEEQASPHQSCVEMMTLHSAKGLEFPRVFMVGVEEGLFPSFRSFEEPGRLEEERRLAYVGITRAKKKLTISYAESRRLYAKEERHLPSRFIAELPRECIQEIRLRGTVTRAMNLAKVGSLSNTSAVENEWKMGQKVKHEKFGFGTVINVEGSENNTRLQIAFQAQGIKWLIAHLAKLEKVR mint 67FD98A7E455677E uvrd-uvrd 2 HiUvrD 4 predetermined predetermined participant predetermined predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified HiUvrD 4 predetermined predetermined participant predetermined predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified direct interaction direct interaction f7a mint UvrD exist predominantly as monomers in solution together with the presence of small amounts of higher oligomeric forms corresponding to dimeric, trimeric, and tetrameric species as estimated from elution profiles of standard proteins To determine the role of C-terminus of UvrD proteins in their oligomerization, HiUvrD C48 and HpUvrD C63 were subjected to gel-filtration analysis under same conditions as the wild-type proteins. HiUvrD C48 eluted as a single peak corresponding to monomeric protein (~ 80 kDa) lacking the oligomeric species (Fig. 7A). This implicates the distal C-terminus of HiUvrD to be responsible for oligomerization. o26013-o26013-1 2 HpUvrD 3 predetermined predetermined participant predetermined predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified HpUvrD 3 predetermined predetermined participant predetermined predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified direct interaction direct interaction f7b mint This indicates that both the UvrDs exist predominantly as monomers in solution together with the presence of small amounts of higher oligomeric forms corresponding to dimeric, trimeric, and tetrameric species as estimated from elution profiles of standard proteins (Fig. 7). uvrd-1 1 HiUvrD 4 weight silver stain molecular weight estimation by silver staining weight silver stain predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified Stoichiometry: 2.0 direct interaction direct interaction f8a mint o26013-2 1 HpUvrD 3 weight silver stain molecular weight estimation by silver staining weight silver stain predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified Stoichiometry: 2.0 direct interaction direct interaction mint f8b