IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ The Crosstalk of mTOR/S6K1 and Hedgehog Pathways. The Crosstalk of mTOR/S6K1 and Hedgehog Pathways. Cancer Cell (1535-6108) 2012 imex curation Wang Y., Ding Q., Yen C.J., Xia W., Izzo J.G., Lang J.Y., Li C.W., Hsu J.L., Miller S.A., Wang X., Lee D.F., Hsu J.M., Huo L., Labaff A.M., Liu D., Huang T.H., Lai C.C., Tsai F.J., Chang W.C., Chen C.H., Wu T.T., Buttar N.S., Wang K.K., Wu Y., Wang H., Ajani J., Hung M.C. Cancer - Interactions investigated in the context of cancer email sent 13/4/12 -recipient away - so no reply expected until early MAY...chasing email sent 30/4/12.- reply obtained to this 1/5/12! Kinase Assay Fig 3A not added = interactors not purified. The authors have confirmed that the constructs "HA-S6K1" and mutants, GST-Gli1F1, Gli1S84A/E, Flag-Gli1S84A and Flag-Gli1 WT are all of human origin. mhung@mdanderson.org 20-Jun-2012: Contacted by JYOTI. Only protein-protein interactions human-be3 Human BE3-esophageal adenocarcinoma cell line be3 Human BE3 - esophageal adenocarcinoma cell line Barrett's esophagus associated esophageal adenocarcinoma from distal esophagus. Expresses: pIκκBeta (S181) pTSC1(S511) p36K1(T389) members of the inflammation associated signaling pathway. anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Wang Y., Ding Q., Yen C.J., Xia W., Izzo J.G., Lang J.Y., Li C.W., Hsu J.L., Miller S.A., Wang X., Lee D.F., Hsu J.M., Huo L., Labaff A.M., Liu D., Huang T.H., Lai C.C., Tsai F.J., Chang W.C., Chen C.H., Wu T.T., Buttar N.S., Wang K.K., Wu Y., Wang H., Ajani J., Hung M.C. Cancer - Interactions investigated in the context of cancer email sent 13/4/12 -recipient away - so no reply expected until early MAY...chasing email sent 30/4/12.- reply obtained to this 1/5/12! The authors have confirmed that the constructs "HA-S6K1" and mutants, GST-Gli1F1, Gli1S84A/E, Flag-Gli1S84A and Flag-Gli1 WT are all of human origin. mhung@mdanderson.org Accepted 2012-MAY-08 by PPORRAS 20-Jun-2012: Contacted by JYOTI. Cancer Cell (1535-6108) 2012 Only protein-protein interactions imex curation The Crosstalk of mTOR/S6K1 and Hedgehog Pathways. The Crosstalk of mTOR/S6K1 and Hedgehog Pathways. Cancer Cell (1535-6108) 2012 imex curation Wang Y., Ding Q., Yen C.J., Xia W., Izzo J.G., Lang J.Y., Li C.W., Hsu J.L., Miller S.A., Wang X., Lee D.F., Hsu J.M., Huo L., Labaff A.M., Liu D., Huang T.H., Lai C.C., Tsai F.J., Chang W.C., Chen C.H., Wu T.T., Buttar N.S., Wang K.K., Wu Y., Wang H., Ajani J., Hung M.C. Cancer - Interactions investigated in the context of cancer email sent 13/4/12 -recipient away - so no reply expected until early MAY...chasing email sent 30/4/12.- reply obtained to this 1/5/12! Kinase Assay Fig 3A not added = interactors not purified. The authors have confirmed that the constructs "HA-S6K1" and mutants, GST-Gli1F1, Gli1S84A/E, Flag-Gli1S84A and Flag-Gli1 WT are all of human origin. mhung@mdanderson.org 20-Jun-2012: Contacted by JYOTI. Only protein-protein interactions human-skgt4 Human SKGT4 skgt4 Human SKGT4 - esophageal adenocarcinoma cell line anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Wang Y., Ding Q., Yen C.J., Xia W., Izzo J.G., Lang J.Y., Li C.W., Hsu J.L., Miller S.A., Wang X., Lee D.F., Hsu J.M., Huo L., Labaff A.M., Liu D., Huang T.H., Lai C.C., Tsai F.J., Chang W.C., Chen C.H., Wu T.T., Buttar N.S., Wang K.K., Wu Y., Wang H., Ajani J., Hung M.C. Cancer - Interactions investigated in the context of cancer email sent 13/4/12 -recipient away - so no reply expected until early MAY...chasing email sent 30/4/12.- reply obtained to this 1/5/12! The authors have confirmed that the constructs "HA-S6K1" and mutants, GST-Gli1F1, Gli1S84A/E, Flag-Gli1S84A and Flag-Gli1 WT are all of human origin. mhung@mdanderson.org Accepted 2012-MAY-08 by PPORRAS 20-Jun-2012: Contacted by JYOTI. Cancer Cell (1535-6108) 2012 Only protein-protein interactions imex curation The Crosstalk of mTOR/S6K1 and Hedgehog Pathways. The Crosstalk of mTOR/S6K1 and Hedgehog Pathways. Cancer Cell (1535-6108) 2012 imex curation Wang Y., Ding Q., Yen C.J., Xia W., Izzo J.G., Lang J.Y., Li C.W., Hsu J.L., Miller S.A., Wang X., Lee D.F., Hsu J.M., Huo L., Labaff A.M., Liu D., Huang T.H., Lai C.C., Tsai F.J., Chang W.C., Chen C.H., Wu T.T., Buttar N.S., Wang K.K., Wu Y., Wang H., Ajani J., Hung M.C. Cancer - Interactions investigated in the context of cancer email sent 13/4/12 -recipient away - so no reply expected until early MAY...chasing email sent 30/4/12.- reply obtained to this 1/5/12! Kinase Assay Fig 3A not added = interactors not purified. The authors have confirmed that the constructs "HA-S6K1" and mutants, GST-Gli1F1, Gli1S84A/E, Flag-Gli1S84A and Flag-Gli1 WT are all of human origin. mhung@mdanderson.org 20-Jun-2012: Contacted by JYOTI. Only protein-protein interactions human-be3 Human BE3-esophageal adenocarcinoma cell line be3 Human BE3 - esophageal adenocarcinoma cell line Barrett's esophagus associated esophageal adenocarcinoma from distal esophagus. Expresses: pIκκBeta (S181) pTSC1(S511) p36K1(T389) members of the inflammation associated signaling pathway. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Wang Y., Ding Q., Yen C.J., Xia W., Izzo J.G., Lang J.Y., Li C.W., Hsu J.L., Miller S.A., Wang X., Lee D.F., Hsu J.M., Huo L., Labaff A.M., Liu D., Huang T.H., Lai C.C., Tsai F.J., Chang W.C., Chen C.H., Wu T.T., Buttar N.S., Wang K.K., Wu Y., Wang H., Ajani J., Hung M.C. Cancer - Interactions investigated in the context of cancer email sent 13/4/12 -recipient away - so no reply expected until early MAY...chasing email sent 30/4/12.- reply obtained to this 1/5/12! The authors have confirmed that the constructs "HA-S6K1" and mutants, GST-Gli1F1, Gli1S84A/E, Flag-Gli1S84A and Flag-Gli1 WT are all of human origin. mhung@mdanderson.org Accepted 2012-MAY-08 by PPORRAS Cancer Cell (1535-6108) 2012 Only protein-protein interactions imex curation The Crosstalk of mTOR/S6K1 and Hedgehog Pathways. The Crosstalk of mTOR/S6K1 and Hedgehog Pathways. Cancer Cell (1535-6108) 2012 imex curation Wang Y., Ding Q., Yen C.J., Xia W., Izzo J.G., Lang J.Y., Li C.W., Hsu J.L., Miller S.A., Wang X., Lee D.F., Hsu J.M., Huo L., Labaff A.M., Liu D., Huang T.H., Lai C.C., Tsai F.J., Chang W.C., Chen C.H., Wu T.T., Buttar N.S., Wang K.K., Wu Y., Wang H., Ajani J., Hung M.C. Cancer - Interactions investigated in the context of cancer email sent 13/4/12 -recipient away - so no reply expected until early MAY...chasing email sent 30/4/12.- reply obtained to this 1/5/12! Kinase Assay Fig 3A not added = interactors not purified. The authors have confirmed that the constructs "HA-S6K1" and mutants, GST-Gli1F1, Gli1S84A/E, Flag-Gli1S84A and Flag-Gli1 WT are all of human origin. mhung@mdanderson.org 20-Jun-2012: Contacted by JYOTI. Only protein-protein interactions in vitro In vitro protein kinase assay protein kinase assay western blot western blot Immuno blot Immuno blot Wang Y., Ding Q., Yen C.J., Xia W., Izzo J.G., Lang J.Y., Li C.W., Hsu J.L., Miller S.A., Wang X., Lee D.F., Hsu J.M., Huo L., Labaff A.M., Liu D., Huang T.H., Lai C.C., Tsai F.J., Chang W.C., Chen C.H., Wu T.T., Buttar N.S., Wang K.K., Wu Y., Wang H., Ajani J., Hung M.C. Cancer - Interactions investigated in the context of cancer email sent 13/4/12 -recipient away - so no reply expected until early MAY...chasing email sent 30/4/12.- reply obtained to this 1/5/12! After transfection with HA-S6K1 or mutants, Be3 cells were lysed and immunoprecipitated with anti-HA antibody. The authors have confirmed that the constructs "HA-S6K1" and mutants, GST-Gli1F1, Gli1S84A/E, Flag-Gli1S84A and Flag-Gli1 WT are all of human origin. mhung@mdanderson.org Accepted 2012-MAY-08 by PPORRAS 20-Jun-2012: Contacted by JYOTI. Cancer Cell (1535-6108) 2012 Only protein-protein interactions imex curation ks6b1_human Ribosomal protein S6 kinase beta-1 RPS6KB1 STK14A Ribosomal protein S6 kinase I p70 ribosomal S6 kinase alpha 70 kDa ribosomal protein S6 kinase 1 Serine/threonine-protein kinase 14A protein protein human Homo sapiens Human MRRRRRRDGFYPAPDFRDREAEDMAGVFDIDLDQPEDAGSEDELEEGGQLNESMDHGGVGPYELGMEHCEKFEISETSVNRGPEKIRPECFELLRVLGKGGYGKVFQVRKVTGANTGKIFAMKVLKKAMIVRNAKDTAHTKAERNILEEVKHPFIVDLIYAFQTGGKLYLILEYLSGGELFMQLEREGIFMEDTACFYLAEISMALGHLHQKGIIYRDLKPENIMLNHQGHVKLTDFGLCKESIHDGTVTHTFCGTIEYMAPEILMRSGHNRAVDWWSLGALMYDMLTGAPPFTGENRKKTIDKILKCKLNLPPYLTQEARDLLKKLLKRNAASRLGAGPGDAGEVQAHPFFRHINWEELLARKVEPPFKPLLQSEEDVSQFDSKFTRQTPVDSPDDSTLSESANQVFLGFTYVAPSVLESVKEKFSFEPKIRSPRRFIGSPRTPVSPVKFSPGDFWGRGASASTANPQTPVEYPMETSGIEQMDVTMSGEASAPLPIRQPNSGPYKKQAFPMISKRPEHLRMNL DIP protein P23443 original sequence version: 163 DIP protein P23443 original sequence version: 88 2C3BA13CCDAF4AB3 p23443-2 Ribosomal protein S6 kinase beta-1 Ribosomal protein S6 kinase I Serine/threonine-protein kinase 14A p70 ribosomal S6 kinase alpha RPS6KB1 STK14A 70 kDa ribosomal protein S6 kinase 1 protein protein human Homo sapiens Human MAGVFDIDLDQPEDAGSEDELEEGGQLNESMDHGGVGPYELGMEHCEKFEISETSVNRGPEKIRPECFELLRVLGKGGYGKVFQVRKVTGANTGKIFAMKVLKKAMIVRNAKDTAHTKAERNILEEVKHPFIVDLIYAFQTGGKLYLILEYLSGGELFMQLEREGIFMEDTACFYLAEISMALGHLHQKGIIYRDLKPENIMLNHQGHVKLTDFGLCKESIHDGTVTHTFCGTIEYMAPEILMRSGHNRAVDWWSLGALMYDMLTGAPPFTGENRKKTIDKILKCKLNLPPYLTQEARDLLKKLLKRNAASRLGAGPGDAGEVQAHPFFRHINWEELLARKVEPPFKPLLQSEEDVSQFDSKFTRQTPVDSPDDSTLSESANQVFLGFTYVAPSVLESVKEKFSFEPKIRSPRRFIGSPRTPVSPVKFSPGDFWGRGASASTANPQTPVEYPMETSGIEQMDVTMSGEASAPLPIRQPNSGPYKKQAFPMISKRPEHLRMNL 2DE6CF3FCABB9FD2 gli1_human Zinc finger protein GLI1 GLI1 GLI Glioma-associated oncogene Oncogene GLI protein protein human Homo sapiens Human MFNSMTPPPISSYGEPCCLRPLPSQGAPSVGTEGLSGPPFCHQANLMSGPHSYGPARETNSCTEGPLFSSPRSAVKLTKKRALSISPLSDASLDLQTVIRTSPSSLVAFINSRCTSPGGSYGHLSIGTMSPSLGFPAQMNHQKGPSPSFGVQPCGPHDSARGGMIPHPQSRGPFPTCQLKSELDMLVGKCREEPLEGDMSSPNSTGIQDPLLGMLDGREDLEREEKREPESVYETDCRWDGCSQEFDSQEQLVHHINSEHIHGERKEFVCHWGGCSRELRPFKAQYMLVVHMRRHTGEKPHKCTFEGCRKSYSRLENLKTHLRSHTGEKPYMCEHEGCSKAFSNASDRAKHQNRTHSNEKPYVCKLPGCTKRYTDPSSLRKHVKTVHGPDAHVTKRHRGDGPLPRAPSISTVEPKREREGGPIREESRLTVPEGAMKPQPSPGAQSSCSSDHSPAGSAANTDSGVEMTGNAGGSTEDLSSLDEGPCIAGTGLSTLRRLENLRLDQLHQLRPIGTRGLKLPSLSHTGTTVSRRVGPPVSLERRSSSSSSISSAYTVSRRSSLASPFPPGSPPENGASSLPGLMPAQHYLLRARYASARGGGTSPTAASSLDRIGGLPMPPWRSRAEYPGYNPNAGVTRRASDPAQAADRPAPARVQRFKSLGCVHTPPTVAGGGQNFDPYLPTSVYSPQPPSITENAAMDARGLQEEPEVGTSMVGSGLNPYMDFPPTDTLGYGGPEGAAAEPYGARGPGSLPLGPGPPTNYGPNPCPQQASYPDPTQETWGEFPSHSGLYPGPKALGGTYSQCPRLEHYGQVQVKPEQGCPVGSDSTGLAPCLNAHPSEGPPHPQPLFSHYPQPSPPQYLQSGPYTQPPPDYLPSEPRPCLDFDSPTHSTGQLKAQLVCNYVQSQQELLWEGGGREDAPAQEPSYQSPKFLGGSQVSPSRAKAPVNTYGPGFGPNLPNHKSGSYPTPSPCHENFVVGANRASHRAAAPPRLLPPLPTCYGPLKVGGTNPSCGHPEVGRLGGGPALYPPPEGQVCNPLDSLDLDNTQLDFVAILDEPQGLSPPPSHDQRGSSGHTPPPSGPPNMAVGNMSVLLRSLPGETEFLNSSA E29B11D1A6CD3D91 sufu_human Suppressor of fused homolog SUFU UNQ650/PRO1280 protein protein human Homo sapiens Human MAELRPSGAPGPTAPPAPGPTAPPAFASLFPPGLHAIYGECRRLYPDQPNPLQVTAIVKYWLGGPDPLDYVSMYRNVGSPSANIPEHWHYISFGLSDLYGDNRVHEFTGTDGPSGFGFELTFRLKRETGESAPPTWPAELMQGLARYVFQSENTFCSGDHVSWHSPLDNSESRIQHMLLTEDPQMQPVQTPFGVVTFLQIVGVCTEELHSAQQWNGQGILELLRTVPIAGGPWLITDMRRGETIFEIDPHLQERVDKGIETDGSNLSGVSAKCAWDDLSRPPEDDEDSRSICIGTQPRRLSGKDTEQIRETLRRGLEINSKPVLPPINPQRQNGLAHDRAPSRKDSLESDSSTAIIPHELIRTRQLESVHLKFNQESGALIPLCLRGRLLHGRHFTYKSITGDMAITFVSTGVEGAFATEEHPYAAHGPWLQILLTEEFVEKMLEDLEDLTSPEEFKLPKEYSWPEKKLKVSILPDVVFDSPLH 4A9CD1CF75FC179A gli1-rps6kb1-1 3 5 unspecified role unspecified role prey prey 7 unspecified role unspecified role bait bait physical association physical association Fig 2A TNF-alpha, no interactions were found between Gli1 and mTOR pathway components in the absence of TNFalpha. Rapamycin gli1-rps6kb1-2 2 5 unspecified role unspecified role prey prey 7 unspecified role unspecified role bait bait physical association physical association Fig 2A TNF-alpha, no interactions were found between Gli1 and mTOR pathway components in the absence of TNFalpha. Rapamycin rps6kb1-gli1 2 5 unspecified role unspecified role bait bait 7 unspecified role unspecified role prey prey physical association physical association Fig S2A TNF-alpha, no interactions were found between Gli1 and mTOR pathway components in the absence of TNFalpha. Rapamycin rps6kb1-gli1-1 3 5 unspecified role unspecified role bait bait 7 unspecified role unspecified role prey prey physical association physical association Fig S2A TNF-alpha, no interactions were found between Gli1 and mTOR pathway components in the absence of TNFalpha. Rapamycin rps6kb1-gli1-2 1 6 unspecified role unspecified role bait bait region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined P23443-2:p.Thr389Ala mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain T A Wed May 02 18:03:46 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'thr389ala' Tue Jun 30 15:45:54 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Thr389Ala' P23443-2:p.Thr389Glu mutation increasing mutation increasing interaction mutation increasing certain certain sequence position certain certain certain sequence position certain T E Wed May 02 18:26:00 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'thr389glu' Tue Jun 30 18:41:23 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Thr389Glu' 7 unspecified role unspecified role prey prey physical association physical association Fig 2B Rapamycin could inhibit the activity of S6K1, but not that of (constitutively activated) S6K1T389E. gli1-rps6kb1-3 4 7 enzyme target enzyme target substrate substrate neutral component neutral component region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined P08151:p.Ser84Ala mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain S A Wed May 02 17:48:12 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser84ala' Tue Jun 30 19:09:31 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser84Ala' region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 6 enzyme enzyme neutral component neutral component P23443-2:p.Thr389Ala mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain T A Wed May 02 19:18:04 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'thr389ala' Tue Jun 30 16:32:07 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Thr389Ala' region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined human-be3 Human BE3-esophageal adenocarcinoma cell line be3 Human BE3 - esophageal adenocarcinoma cell line Barrett's esophagus associated esophageal adenocarcinoma from distal esophagus. Expresses: pIκκBeta (S181) pTSC1(S511) p36K1(T389) members of the inflammation associated signaling pathway. phosphorylation phosphorylation reaction phosphorylation Fig 3B, 3D Rapamycin could inhibit the activity of S6K1, but not that of (constitutively activated) S6K1T389E. gli1-sufu 3 7 unspecified role unspecified role bait bait 8 unspecified role unspecified role prey prey physical association physical association Fig 5E sufu-gli1-1 3 8 unspecified role unspecified role bait bait 7 unspecified role unspecified role prey prey P08151:p.Ser84Glu mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain S E Wed May 02 16:42:35 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser84glu' Tue Jun 30 17:54:33 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser84Glu' physical association physical association Fig 5E sufu-gli1-2 3 7 unspecified role unspecified role prey prey 8 unspecified role unspecified role bait bait physical association physical association Fig 5F Rapamycin TNF-alpha and S6K1. Both TNF-a treatment and S6K1 ectopic expression decreased the binding between SuFu and Gli1, which was fully reversed by rapamycin. sufu-gli1-3 3 8 unspecified role unspecified role bait bait 7 unspecified role unspecified role prey prey P08151:p.Ser84Glu mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain S E Wed May 02 16:35:13 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser84glu' Tue Jun 30 16:29:55 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser84Glu' region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Fig S5K gli1-sufu-7 1 7 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 8 unspecified role unspecified role prey prey physical association physical association Fig S5K