InnateDB InnateDB Dectin-1 is an extracellular pathogen sensor for the induction and processing of IL-1β via a noncanonical caspase-8 inflammasome. Dectin-1 is an extracellular pathogen sensor for the induction and processing of IL-1β via a noncanonical caspase-8 inflammasome. Nat. Immunol. (1529-2908) 2012 imex curation Gringhuis S.I., Kaptein T.M., Wevers B.A., Theelen B., van der Vlist M., Boekhout T., Geijtenbeek T.B., Gringhuis S.I., Kaptein T.M., Wevers B.A., Theelen B., van der Vlist M., Boekhout T., Geijtenbeek T.B. Curdlan is a (1,3)-beta-glucan, a PAMP recognised by Dectin-1. Piceatannol is a Syk inhibitor. s.i.ringhuis@amc.uva.nl,t .b.geijtenbeek@amc.uva.nl t.b.geijtenbeek@amc.uva.nl, t.b.geijtenbeek@amc.uva.nl 19-Sept-2012: Contacted by IntAct-Help. Only protein-protein interactions Curated by: InnateDB - a knowledge resource for innate immunity human-mddc Human Monocyte-derived dendritic cell monocyte-derived_dendritic_cell Monocyte-derived dendritic cell (MDDC) anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Gringhuis S.I., Kaptein T.M., Wevers B.A., Theelen B., van der Vlist M., Boekhout T., Geijtenbeek T.B. Curdlan is a (1,3)-beta-glucan, a PAMP recognised by Dectin-1. Piceatannol is a Syk inhibitor. s.i.ringhuis@amc.uva.nl,t .b.geijtenbeek@amc.uva.nl t.b.geijtenbeek@amc.uva.nl, t.b.geijtenbeek@amc.uva.nl Accepted 2012-AUG-06 by MGT 19-Sept-2012: Contacted by IntAct-Help. Curdlan (ug/ml) Piceatannol (uM) Nat. Immunol. (1529-2908) 2012 Only protein-protein interactions imex curation Curated by: InnateDB - a knowledge resource for innate immunity Dectin-1 is an extracellular pathogen sensor for the induction and processing of IL-1β via a noncanonical caspase-8 inflammasome. Dectin-1 is an extracellular pathogen sensor for the induction and processing of IL-1β via a noncanonical caspase-8 inflammasome. Nat. Immunol. (1529-2908) 2012 imex curation Gringhuis S.I., Kaptein T.M., Wevers B.A., Theelen B., van der Vlist M., Boekhout T., Geijtenbeek T.B., Gringhuis S.I., Kaptein T.M., Wevers B.A., Theelen B., van der Vlist M., Boekhout T., Geijtenbeek T.B. Curdlan is a (1,3)-beta-glucan, a PAMP recognised by Dectin-1. Piceatannol is a Syk inhibitor. s.i.ringhuis@amc.uva.nl,t .b.geijtenbeek@amc.uva.nl t.b.geijtenbeek@amc.uva.nl, t.b.geijtenbeek@amc.uva.nl 19-Sept-2012: Contacted by IntAct-Help. Only protein-protein interactions Curated by: InnateDB - a knowledge resource for innate immunity human-mddc Human Monocyte-derived dendritic cell monocyte-derived_dendritic_cell Monocyte-derived dendritic cell (MDDC) anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Gringhuis S.I., Kaptein T.M., Wevers B.A., Theelen B., van der Vlist M., Boekhout T., Geijtenbeek T.B. Curdlan is a (1,3)-beta-glucan, a PAMP recognised by Dectin-1. Piceatannol is a Syk inhibitor. s.i.ringhuis@amc.uva.nl,t .b.geijtenbeek@amc.uva.nl t.b.geijtenbeek@amc.uva.nl, t.b.geijtenbeek@amc.uva.nl 19-Sept-2012: Contacted by IntAct-Help. Nat. Immunol. (1529-2908) 2012 Only protein-protein interactions imex curation Curated by: InnateDB - a knowledge resource for innate immunity Dectin-1 is an extracellular pathogen sensor for the induction and processing of IL-1β via a noncanonical caspase-8 inflammasome. Dectin-1 is an extracellular pathogen sensor for the induction and processing of IL-1β via a noncanonical caspase-8 inflammasome. Nat. Immunol. (1529-2908) 2012 imex curation Gringhuis S.I., Kaptein T.M., Wevers B.A., Theelen B., van der Vlist M., Boekhout T., Geijtenbeek T.B., Gringhuis S.I., Kaptein T.M., Wevers B.A., Theelen B., van der Vlist M., Boekhout T., Geijtenbeek T.B. Curdlan is a (1,3)-beta-glucan, a PAMP recognised by Dectin-1. Piceatannol is a Syk inhibitor. s.i.ringhuis@amc.uva.nl,t .b.geijtenbeek@amc.uva.nl t.b.geijtenbeek@amc.uva.nl, t.b.geijtenbeek@amc.uva.nl 19-Sept-2012: Contacted by IntAct-Help. Only protein-protein interactions Curated by: InnateDB - a knowledge resource for innate immunity human-mddc Human Monocyte-derived dendritic cell monocyte-derived_dendritic_cell Monocyte-derived dendritic cell (MDDC) anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Gringhuis S.I., Kaptein T.M., Wevers B.A., Theelen B., van der Vlist M., Boekhout T., Geijtenbeek T.B. Curdlan is a (1,3)-beta-glucan, a PAMP recognised by Dectin-1. Piceatannol is a Syk inhibitor. s.i.ringhuis@amc.uva.nl,t .b.geijtenbeek@amc.uva.nl t.b.geijtenbeek@amc.uva.nl, t.b.geijtenbeek@amc.uva.nl 19-Sept-2012: Contacted by IntAct-Help. Nat. Immunol. (1529-2908) 2012 Only protein-protein interactions imex curation Curated by: InnateDB - a knowledge resource for innate immunity Dectin-1 is an extracellular pathogen sensor for the induction and processing of IL-1β via a noncanonical caspase-8 inflammasome. Dectin-1 is an extracellular pathogen sensor for the induction and processing of IL-1β via a noncanonical caspase-8 inflammasome. Nat. Immunol. (1529-2908) 2012 imex curation Gringhuis S.I., Kaptein T.M., Wevers B.A., Theelen B., van der Vlist M., Boekhout T., Geijtenbeek T.B., Gringhuis S.I., Kaptein T.M., Wevers B.A., Theelen B., van der Vlist M., Boekhout T., Geijtenbeek T.B. Curdlan is a (1,3)-beta-glucan, a PAMP recognised by Dectin-1. Piceatannol is a Syk inhibitor. s.i.ringhuis@amc.uva.nl,t .b.geijtenbeek@amc.uva.nl t.b.geijtenbeek@amc.uva.nl, t.b.geijtenbeek@amc.uva.nl 19-Sept-2012: Contacted by IntAct-Help. Only protein-protein interactions Curated by: InnateDB - a knowledge resource for innate immunity human-mddc Human Monocyte-derived dendritic cell monocyte-derived_dendritic_cell Monocyte-derived dendritic cell (MDDC) anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Gringhuis S.I., Kaptein T.M., Wevers B.A., Theelen B., van der Vlist M., Boekhout T., Geijtenbeek T.B. Curdlan is a (1,3)-beta-glucan, a PAMP recognised by Dectin-1. Piceatannol is a Syk inhibitor. s.i.ringhuis@amc.uva.nl,t .b.geijtenbeek@amc.uva.nl t.b.geijtenbeek@amc.uva.nl, t.b.geijtenbeek@amc.uva.nl 19-Sept-2012: Contacted by IntAct-Help. Curdlan (ug/ml) Piceatannol (uM) Nat. Immunol. (1529-2908) 2012 Only protein-protein interactions imex curation Curated by: InnateDB - a knowledge resource for innate immunity malt1_human Mucosa-associated lymphoid tissue lymphoma translocation protein 1 MALT lymphoma-associated translocation Paracaspase MALT1 MLT protein protein human Homo sapiens Human MSLLGDPLQALPPSAAPTGPLLAPPAGATLNRLREPLLRRLSELLDQAPEGRGWRRLAELAGSRGRLRLSCLDLEQCSLKVLEPEGSPSLCLLKLMGEKGCTVTELSDFLQAMEHTEVLQLLSPPGIKITVNPESKAVLAGQFVKLCCRATGHPFVQYQWFKMNKEIPNGNTSELIFNAVHVKDAGFYVCRVNNNFTFEFSQWSQLDVCDIPESFQRSVDGVSESKLQICVEPTSQKLMPGSTLVLQCVAVGSPIPHYQWFKNELPLTHETKKLYMVPYVDLEHQGTYWCHVYNDRDSQDSKKVEIIIGRTDEAVECTEDELNNLGHPDNKEQTTDQPLAKDKVALLIGNMNYREHPKLKAPLVDVYELTNLLRQLDFKVVSLLDLTEYEMRNAVDEFLLLLDKGVYGLLYYAGHGYENFGNSFMVPVDAPNPYRSENCLCVQNILKLMQEKETGLNVFLLDMCRKRNDYDDTIPILDALKVTANIVFGYATCQGAEAFEIQHSGLANGIFMKFLKDRLLEDKKITVLLDEVAEDMGKCHLTKGKQALEIRSSLSEKRALTDPIQGTEYSAESLVRNLQWAKAHELPESMCLKFDCGVQIQLGFAAEFSNVMIIYTSIVYKPPEIIMCDAYVTDFPLDLDIDPKDANKGTPEETGSYLVSKDLPKHCLYTRLSSLQKLKEHLVFTVCLSYQYSGLEDTVEDKQEVNVGKPLIAKLDMHRGLGRKTCFQTCLMSNGPYQSSAATSGGAGHYHSLQDPFHGVYHSHPGNPSNVTPADSCHCSRTPDAFISSFAHHASCHFSRSNVPVETTDEIPFSFSDRLRISEK DIP protein Q9UDY8 original sequence version: 90 DIP protein Q9UDY8-1 original sequence version: 129 28AFB80DA025F8AB casp8_human Caspase-8 CASP8 MCH5 ICE-like apoptotic protease 5 MORT1-associated ced-3 homolog FADD-homologous ICE/ced-3-like protease FADD-like ICE Apoptotic cysteine protease Apoptotic protease Mch-5 CAP4 protein protein human Homo sapiens Human MDFSRNLYDIGEQLDSEDLASLKFLSLDYIPQRKQEPIKDALMLFQRLQEKRMLEESNLSFLKELLFRINRLDLLITYLNTRKEEMERELQTPGRAQISAYRVMLYQISEEVSRSELRSFKFLLQEEISKCKLDDDMNLLDIFIEMEKRVILGEGKLDILKRVCAQINKSLLKIINDYEEFSKERSSSLEGSPDEFSNGEELCGVMTISDSPREQDSESQTLDKVYQMKSKPRGYCLIINNHNFAKAREKVPKLHSIRDRNGTHLDAGALTTTFEELHFEIKPHDDCTVEQIYEILKIYQLMDHSNMDCFICCILSHGDKGIIYGTDGQEAPIYELTSQFTGLKCPSLAGKPKVFFIQACQGDNYQKGIPVETDSEEQPYLEMDLSSPQTRYIPDEADFLLGMATVNNCVSYRNPAEGTWYIQSLCQSLRERCPRGDDILTILTEVNYEVSNKDDKKNMGKQMPQPTFTLRKKLVFPSD DIP protein Q14790 original sequence version: 184 DIP protein Q14790 original sequence version: 135 DIP protein Q14790 original sequence version: 136 DIP protein Q14790 original sequence version: 147 DIP protein Q14790 original sequence version: 146 DIP protein Q14790 original sequence version: 170 DIP protein Q14790 original sequence version: 198 DIP protein Q14790-1 original sequence version: 197 DIP protein Q14790 original sequence version: 158 7A5FEAA6B39B582F card9_human Caspase recruitment domain-containing protein 9 CARD9 protein protein human Homo sapiens Human MSDYENDDECWSVLEGFRVTLTSVIDPSRITPYLRQCKVLNPDDEEQVLSDPNLVIRKRKVGVLLDILQRTGHKGYVAFLESLELYYPQLYKKVTGKEPARVFSMIIDASGESGLTQLLMTEVMKLQKKVQDLTALLSSKDDFIKELRVKDSLLRKHQERVQRLKEECEAGSRELKRCKEENYDLAMRLAHQSEEKGAALMRNRDLQLEIDQLKHSLMKAEDDCKVERKHTLKLRHAMEQRPSQELLWELQQEKALLQARVQELEASVQEGKLDRSSPYIQVLEEDWRQALRDHQEQANTIFSLRKDLRQGEARRLRCMEEKEMFELQCLALRKDSKMYKDRIEAILLQMEEVAIERDQAIATREELHAQHARGLQEKDALRKQVRELGEKADELQLQVFQCEAQLLAVEGRLRRQQLETLVLSSDLEDGSPRRSQELSLPQDLEDTQLSDKGCLAGGGSPKQPFAALHQEQVLRNPHDAGLSSGEPPEKERRRLKESFENYRRKRALRKMQKGWRQGEEDRENTTGSDNTDTEGS 6EB18353112F2BAC asc_human Apoptosis-associated speck-like protein containing a CARD PYD and CARD domain-containing protein Target of methylation-induced silencing 1 Caspase recruitment domain-containing protein 5 ASC CARD5 TMS1 PYCARD protein protein human Homo sapiens Human MGRARDAILDALENLTAEELKKFKLKLLSVPLREGYGRIPRGALLSMDALDLTDKLVSFYLETYGAELTANVLRDMGLQEMAGQLQAATHQGSGAAPAGIQAPPQSAAKPGLHFIDQHRAALIARVTNVEWLLDALYGKVLTDEQYQAVRAEPTNPSKMRKLFSFTPAWNWTCKDLLLQALRESQSYLVEDLERS DIP protein Q9ULZ3 original sequence version: 116 455987286586F46A bcl10_human B-cell lymphoma/leukemia 10 BCL10 CIPER CLAP Cellular homolog of vCARMEN Mammalian CARD-containing adapter molecule E10 Cellular-E10 CARD-like apoptotic protein B-cell CLL/lymphoma 10 CED-3/ICH-1 prodomain homologous E10-like regulator CARD-containing molecule enhancing NF-kappa-B protein protein human Homo sapiens Human MEPTAPSLTEEDLTEVKKDALENLRVYLCEKIIAERHFDHLRAKKILSREDTEEISCRTSSRKRAGKLLDYLQENPKGLDTLVESIRREKTQNFLIQKITDEVLKLRNIKLEHLKGLKCSSCEPFPDGATNNLSRSNSDESNFSEKLRASTVMYHPEGESSTTPFFSTNSSLNLPVLEVGRTENTIFSSTTLPRPGDPGAPPLPPDLQLEEEGTCANSSEMFLPLRSRTVSRQ DIP protein O95999 original sequence version: 69 DIP protein O95999 original sequence version: 122 DIP protein O95999 original sequence version: 60 DIP protein O95999 original sequence version: 86 F87C97F2B784BA4B bcl10-card9 1 7 unspecified role unspecified role prey prey 9 unspecified role unspecified role prey prey 5 unspecified role unspecified role bait bait 6 unspecified role unspecified role prey prey 8 unspecified role unspecified role prey prey association association Fig. 3A, 3J. curdlan piceatannol Association of CARD9 and ASC is dependent on curdlan stimulation in the absence of piceatannol. 10 0 bcl10-card9-1 4 5 unspecified role unspecified role prey prey 7 unspecified role unspecified role prey prey 6 unspecified role unspecified role prey prey 9 unspecified role unspecified role bait bait association association Fig. 3B. curdlan piceatannol Association of CARD9 and Casp8 is dependent on curdlan stimulation in the absence of piceatannol. 0 10 card9-bcl10 2 7 unspecified role unspecified role bait bait 5 unspecified role unspecified role prey prey 9 unspecified role unspecified role prey prey 6 unspecified role unspecified role prey prey association association Fig. 3C. curdlan CARD9 was not associated with MALT1–caspase-8 or MALT1–Bcl-10 before dectin-1 triggering. However, stimulation with curdlan resulted in CARD9 immunoprecipitating together with MALT1 and Bcl-10 as well as with caspase-8, and this was dependent on Syk activation. bcl10-card9-2 3 7 unspecified role unspecified role prey prey 9 unspecified role unspecified role prey prey 8 unspecified role unspecified role prey prey 5 unspecified role unspecified role prey prey 6 unspecified role unspecified role bait bait association association Fig. 3D. curdlan piceatannol Association of Bcl-10, CARD9 and ASC is dependent on curdlan stimulation in the absence of piceatannol. 0 10 bcl10-card9-3 2 6 unspecified role unspecified role prey prey 9 unspecified role unspecified role prey prey 8 unspecified role unspecified role bait bait 7 unspecified role unspecified role prey prey 5 unspecified role unspecified role prey prey association association Fig. 3I. curdlan ASC immunoprecipitated together with MALT1, caspase-8, CARD9 and Bcl-10 in whole-cell extracts of curdlan-stimulated DCs but not in those of unstimulated DCs. pycard-casp8 2 5 unspecified role unspecified role prey prey 8 unspecified role unspecified role bait bait 6 unspecified role unspecified role prey prey association association Fig. 3K. curdlan After triggering of dectin-1, ASC did not associate with caspase-8 in extracts of cells in which MALT1 was silenced. casp8-malt1 2 5 unspecified role unspecified role prey prey 8 unspecified role unspecified role prey prey 6 unspecified role unspecified role bait bait association association Fig. 3K-2 curdlan malt1-bcl10 1 6 unspecified role unspecified role prey prey 5 unspecified role unspecified role bait bait 9 unspecified role unspecified role prey prey association association Fig. 3A, 3J -2 curdlan piceatannol Association of CARD9 and ASC is dependent on curdlan stimulation in the absence of piceatannol. 0 40 malt1-bcl10-1 1 6 unspecified role unspecified role prey prey 5 unspecified role unspecified role bait bait 9 unspecified role unspecified role prey prey association association Fig. 3A, 3J -3 curdlan piceatannol Association of CARD9 and ASC is dependent on curdlan stimulation in the absence of piceatannol. 0 0 bcl10-card9-4 4 7 unspecified role unspecified role prey prey 5 unspecified role unspecified role prey prey 9 unspecified role unspecified role bait bait association association Fig. 3B-2 curdlan piceatannol Association of CARD9 and Casp8 is dependent on curdlan stimulation in the absence of piceatannol. 0 0 bcl10-card9-5 4 9 unspecified role unspecified role bait bait 5 unspecified role unspecified role prey prey 7 unspecified role unspecified role prey prey association association Fig. 3B-3 curdlan piceatannol Association of CARD9 and Casp8 is dependent on curdlan stimulation in the absence of piceatannol. 40 0 casp8-bcl10 3 5 unspecified role unspecified role prey prey 6 unspecified role unspecified role bait bait physical association physical association Fig. 3D-2 curdlan piceatannol Association of Bcl-10, CARD9 and ASC is dependent on curdlan stimulation in the absence of piceatannol. 0 0 casp8-malt1-1 3 6 unspecified role unspecified role bait bait 5 unspecified role unspecified role prey prey physical association physical association Fig. 3D-3 curdlan piceatannol Association of Bcl-10, CARD9 and ASC is dependent on curdlan stimulation in the absence of piceatannol. 40 0 casp8-malt1-2 2 6 unspecified role unspecified role bait bait 5 unspecified role unspecified role prey prey physical association physical association Fig. 3K-3 curdlan ASC did not associate with caspase-8 in extracts of cells in which MALT1 was silenced. malt1-bcl10-2 1 6 unspecified role unspecified role prey prey 9 unspecified role unspecified role prey prey 5 unspecified role unspecified role bait bait association association Fig. 3A, 3J -2 curdlan piceatannol Association of CARD9 and ASC is dependent on curdlan stimulation in the absence of piceatannol. 10 40 bcl10-card9-6 4 5 unspecified role unspecified role prey prey 9 unspecified role unspecified role bait bait 7 unspecified role unspecified role prey prey association association Fig. 3B-3 curdlan piceatannol Association of CARD9 and Casp8 is dependent on curdlan stimulation in the absence of piceatannol. 40 10 casp8-malt1-3 3 6 unspecified role unspecified role bait bait 5 unspecified role unspecified role prey prey physical association physical association Fig. 3D-3 curdlan piceatannol Association of Bcl-10, CARD9 and ASC is dependent on curdlan stimulation in the absence of piceatannol. 40 10