IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Loss of Tankyrase-Mediated Destruction of 3BP2 Is the Underlying Pathogenic Mechanism of Cherubism. Loss of Tankyrase-Mediated Destruction of 3BP2 Is the Underlying Pathogenic Mechanism of Cherubism. Cell (0092-8674) 2011 imex curation Levaot N., Voytyuk O., Dimitriou I., Sircoulomb F., Chandrakumar A., Deckert M., Krzyzanowski P.M., Scotter A., Gu S., Janmohamed S., Cong F., Simoncic P.D., Ueki Y., La Rose J., Rottapel R. Ribosylation assays not added as these were ex-vivo not in vitro. Also not src/syk autophosporylation assay since purity of src/syk not demonstrated. Species of origin of RNF146 taken from related publication PMID: 21478859. Interactions invovling PAK could not be added as the origin of this protein could not be traced. Rottapel@uhnres.utoronto.ca 14-Mar-2012: Contacted by JYOTI. Only protein-protein interactions human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. anti bait coip anti bait coimmunoprecipitation anti bait coip anti tag western anti tag western blot anti tag western Levaot N., Voytyuk O., Dimitriou I., Sircoulomb F., Chandrakumar A., Deckert M., Krzyzanowski P.M., Scotter A., Gu S., Janmohamed S., Cong F., Simoncic P.D., Ueki Y., La Rose J., Rottapel R. Ribosylation assays not added as these were ex-vivo not in vitro. Also not src/syk autophosporylation assay since purity of src/syk not demonstrated. Species of origin of RNF146 taken from related publication PMID: 21478859. Interactions invovling PAK could not be added as the origin of this protein could not be traced. Rottapel@uhnres.utoronto.ca Accepted 2012-FEB-02 by MGT 14-Mar-2012: Contacted by JYOTI. Cell (0092-8674) 2011 Only protein-protein interactions imex curation Loss of Tankyrase-Mediated Destruction of 3BP2 Is the Underlying Pathogenic Mechanism of Cherubism. Loss of Tankyrase-Mediated Destruction of 3BP2 Is the Underlying Pathogenic Mechanism of Cherubism. Cell (0092-8674) 2011 imex curation Levaot N., Voytyuk O., Dimitriou I., Sircoulomb F., Chandrakumar A., Deckert M., Krzyzanowski P.M., Scotter A., Gu S., Janmohamed S., Cong F., Simoncic P.D., Ueki Y., La Rose J., Rottapel R. Ribosylation assays not added as these were ex-vivo not in vitro. Also not src/syk autophosporylation assay since purity of src/syk not demonstrated. Species of origin of RNF146 taken from related publication PMID: 21478859. Interactions invovling PAK could not be added as the origin of this protein could not be traced. Rottapel@uhnres.utoronto.ca 14-Mar-2012: Contacted by JYOTI. Only protein-protein interactions in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Levaot N., Voytyuk O., Dimitriou I., Sircoulomb F., Chandrakumar A., Deckert M., Krzyzanowski P.M., Scotter A., Gu S., Janmohamed S., Cong F., Simoncic P.D., Ueki Y., La Rose J., Rottapel R. Ribosylation assays not added as these were ex-vivo not in vitro. Also not src/syk autophosporylation assay since purity of src/syk not demonstrated. Species of origin of RNF146 taken from related publication PMID: 21478859. Interactions invovling PAK could not be added as the origin of this protein could not be traced. Rottapel@uhnres.utoronto.ca Accepted 2012-FEB-02 by MGT 14-Mar-2012: Contacted by JYOTI. Cell (0092-8674) 2011 Only protein-protein interactions imex curation Loss of Tankyrase-Mediated Destruction of 3BP2 Is the Underlying Pathogenic Mechanism of Cherubism. Loss of Tankyrase-Mediated Destruction of 3BP2 Is the Underlying Pathogenic Mechanism of Cherubism. Cell (0092-8674) 2011 imex curation Levaot N., Voytyuk O., Dimitriou I., Sircoulomb F., Chandrakumar A., Deckert M., Krzyzanowski P.M., Scotter A., Gu S., Janmohamed S., Cong F., Simoncic P.D., Ueki Y., La Rose J., Rottapel R. Ribosylation assays not added as these were ex-vivo not in vitro. Also not src/syk autophosporylation assay since purity of src/syk not demonstrated. Species of origin of RNF146 taken from related publication PMID: 21478859. Interactions invovling PAK could not be added as the origin of this protein could not be traced. Rottapel@uhnres.utoronto.ca 14-Mar-2012: Contacted by JYOTI. Only protein-protein interactions human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Levaot N., Voytyuk O., Dimitriou I., Sircoulomb F., Chandrakumar A., Deckert M., Krzyzanowski P.M., Scotter A., Gu S., Janmohamed S., Cong F., Simoncic P.D., Ueki Y., La Rose J., Rottapel R. Ribosylation assays not added as these were ex-vivo not in vitro. Also not src/syk autophosporylation assay since purity of src/syk not demonstrated. Species of origin of RNF146 taken from related publication PMID: 21478859. Interactions invovling PAK could not be added as the origin of this protein could not be traced. Rottapel@uhnres.utoronto.ca Accepted 2012-FEB-02 by MGT Cell (0092-8674) 2011 Only protein-protein interactions imex curation tnks2_human Poly [ADP-ribose] polymerase tankyrase-2 TNKS2 PARP5B TANK2 TNKL Poly [ADP-ribose] polymerase 5B TNKS-2 TRF1-interacting ankyrin-related ADP-ribose polymerase 2 Tankyrase II Tankyrase-like protein Tankyrase-related protein ADP-ribosyltransferase diphtheria toxin-like 6 Protein poly-ADP-ribosyltransferase tankyrase-2 Tankyrase-2 protein protein human Homo sapiens Human MSGRRCAGGGAACASAAAEAVEPAARELFEACRNGDVERVKRLVTPEKVNSRDTAGRKSTPLHFAAGFGRKDVVEYLLQNGANVQARDDGGLIPLHNACSFGHAEVVNLLLRHGADPNARDNWNYTPLHEAAIKGKIDVCIVLLQHGAEPTIRNTDGRTALDLADPSAKAVLTGEYKKDELLESARSGNEEKMMALLTPLNVNCHASDGRKSTPLHLAAGYNRVKIVQLLLQHGADVHAKDKGDLVPLHNACSYGHYEVTELLVKHGACVNAMDLWQFTPLHEAASKNRVEVCSLLLSYGADPTLLNCHNKSAIDLAPTPQLKERLAYEFKGHSLLQAAREADVTRIKKHLSLEMVNFKHPQTHETALHCAAASPYPKRKQICELLLRKGANINEKTKEFLTPLHVASEKAHNDVVEVVVKHEAKVNALDNLGQTSLHRAAYCGHLQTCRLLLSYGCDPNIISLQGFTALQMGNENVQQLLQEGISLGNSEADRQLLEAAKAGDVETVKKLCTVQSVNCRDIEGRQSTPLHFAAGYNRVSVVEYLLQHGADVHAKDKGGLVPLHNACSYGHYEVAELLVKHGAVVNVADLWKFTPLHEAAAKGKYEICKLLLQHGADPTKKNRDGNTPLDLVKDGDTDIQDLLRGDAALLDAAKKGCLARVKKLSSPDNVNCRDTQGRHSTPLHLAAGYNNLEVAEYLLQHGADVNAQDKGGLIPLHNAASYGHVDVAALLIKYNACVNATDKWAFTPLHEAAQKGRTQLCALLLAHGADPTLKNQEGQTPLDLVSADDVSALLTAAMPPSALPSCYKPQVLNGVRSPGATADALSSGPSSPSSLSAASSLDNLSGSFSELSSVVSSSGTEGASSLEKKEVPGVDFSITQFVRNLGLEHLMDIFEREQITLDVLVEMGHKELKEIGINAYGHRHKLIKGVERLISGQQGLNPYLTLNTSGSGTILIDLSPDDKEFQSVEEEMQSTVREHRDGGHAGGIFNRYNILKIQKVCNKKLWERYTHRRKEVSEENHNHANERMLFHGSPFVNAIIHKGFDERHAYIGGMFGAGIYFAENSSKSNQYVYGIGGGTGCPVHKDRSCYICHRQLLFCRVTLGKSFLQFSAMKMAHSPPGHHSVTGRPSVNGLALAEYVIYRGEQAYPEYLITYQIMRPEGMVDG DIP protein Q9H2K2 original sequence version: 154 DIP protein Q9H2K2 original sequence version: 107 4C8B3B8D97CEF704 rn146_human E3 ubiquitin-protein ligase RNF146 Dactylidin RNF146 RING finger protein 146 Iduna RING-type E3 ubiquitin transferase RNF146 protein protein human Homo sapiens Human MMAGCGEIDHSINMLPTNRKANESCSNTAPSLTVPECAICLQTCVHPVSLPCKHVFCYLCVKGASWLGKRCALCRQEIPEDFLDKPTLLSPEELKAASRGNGEYAWYYEGRNGWWQYDERTSRELEDAFSKGKKNTEMLIAGFLYVADLENMVQYRRNEHGRRRKIKRDIIDIPKKGVAGLRLDCDANTVNLARESSADGADSVSAQSGASVQPLVSSVRPLTSVDGQLTSPATPSPDASTSLEDSFAHLQLSGDNTAERSHRGEGEEDHESPSSGRVPAPDTSIEETESDASSDSEDVSAVVAQHSLTQQRLLVSNANQTVPDRSDRSGTDRSVAGGGTVSVSVRSRRPDGQCTVTEV DIP protein Q9NTX7 original sequence version: 118 7337C410EDA30A7E tnks1_human Poly [ADP-ribose] polymerase tankyrase-1 TNKS PARP5A PARPL TIN1 TINF1 TNKS1 Tankyrase I TNKS-1 TRF1-interacting ankyrin-related ADP-ribose polymerase Poly [ADP-ribose] polymerase 5A ADP-ribosyltransferase diphtheria toxin-like 5 Protein poly-ADP-ribosyltransferase tankyrase-1 Tankyrase-1 protein protein human Homo sapiens Human MAASRRSQHHHHHHQQQLQPAPGASAPPPPPPPPLSPGLAPGTTPASPTASGLAPFASPRHGLALPEGDGSRDPPDRPRSPDPVDGTSCCSTTSTICTVAAAPVVPAVSTSSAAGVAPNPAGSGSNNSPSSSSSPTSSSSSSPSSPGSSLAESPEAAGVSSTAPLGPGAAGPGTGVPAVSGALRELLEACRNGDVSRVKRLVDAANVNAKDMAGRKSSPLHFAAGFGRKDVVEHLLQMGANVHARDDGGLIPLHNACSFGHAEVVSLLLCQGADPNARDNWNYTPLHEAAIKGKIDVCIVLLQHGADPNIRNTDGKSALDLADPSAKAVLTGEYKKDELLEAARSGNEEKLMALLTPLNVNCHASDGRKSTPLHLAAGYNRVRIVQLLLQHGADVHAKDKGGLVPLHNACSYGHYEVTELLLKHGACVNAMDLWQFTPLHEAASKNRVEVCSLLLSHGADPTLVNCHGKSAVDMAPTPELRERLTYEFKGHSLLQAAREADLAKVKKTLALEIINFKQPQSHETALHCAVASLHPKRKQVTELLLRKGANVNEKNKDFMTPLHVAAERAHNDVMEVLHKHGAKMNALDTLGQTALHRAALAGHLQTCRLLLSYGSDPSIISLQGFTAAQMGNEAVQQILSESTPIRTSDVDYRLLEASKAGDLETVKQLCSSQNVNCRDLEGRHSTPLHFAAGYNRVSVVEYLLHHGADVHAKDKGGLVPLHNACSYGHYEVAELLVRHGASVNVADLWKFTPLHEAAAKGKYEICKLLLKHGADPTKKNRDGNTPLDLVKEGDTDIQDLLRGDAALLDAAKKGCLARVQKLCTPENINCRDTQGRNSTPLHLAAGYNNLEVAEYLLEHGADVNAQDKGGLIPLHNAASYGHVDIAALLIKYNTCVNATDKWAFTPLHEAAQKGRTQLCALLLAHGADPTMKNQEGQTPLDLATADDIRALLIDAMPPEALPTCFKPQATVVSASLISPASTPSCLSAASSIDNLTGPLAELAVGGASNAGDGAAGTERKEGEVAGLDMNISQFLKSLGLEHLRDIFETEQITLDVLADMGHEELKEIGINAYGHRHKLIKGVERLLGGQQGTNPYLTFHCVNQGTILLDLAPEDKEYQSVEEEMQSTIREHRDGGNAGGIFNRYNVIRIQKVVNKKLRERFCHRQKEVSEENHNHHNERMLFHGSPFINAIIHKGFDERHAYIGGMFGAGIYFAENSSKSNQYVYGIGGGTGCPTHKDRSCYICHRQMLFCRVTLGKSFLQFSTMKMAHAPPGHHSVIGRPSVNGLAYAEYVIYRGEQAYPEYLITYQIMKPEAPSQTATAAEQKT DIP protein O95271 original sequence version: 148 DIP protein O95271 original sequence version: 149 DIP protein O95271-1 original sequence version: 113 44BDE985C715BEFF 3bp2_mouse SH3 domain-binding protein 2 Sh3bp2 3bp2 protein protein mouse Mus musculus Mouse MAAEEMQWPVPMKAIGAQNLLTMPGGVAKAGYLHKKGGTQLQLLKWPLRFVIIHKRCIYYFKSSTSASPQGAFSLSGYNRVMRAAEETTSNNVFPFKIIHISKKHRTWFFSASSEDERKSWMAFVRREIGHFHEKKELPLDTSDSSSDTDSFYGAVERPIDISLSSYPMDNEDYEHEDEDDSYLEPDSPGPMKLEDALTYPPAYPPPPVPVPRKPAFSDLPRAHSFTSKSPSPLLPPPPPKRGLPDTGSAPEDAKDALGLRRVEPGLRVPATPRRMSDPPMSNVPTVPNLRKHPCFRDSVNPGLEPWTPGHGTSSVSSSTTMAVATSRNCDKLKSFHLSSRGPPTSEPPPVPANKPKFLKIAEEPSPREAAKFAPVPPVAPRPPVQKMPMPEATVRPAVLPRPENTPLPHLQRSPPDGQSFRGFSFEKARQPSQADTGEEDSDEDYEKVPLPNSVFVNTTESCEVERLFKATDPRGEPQDGLYCIRNSSTKSGKVLVVWDESSNKVRNYRIFEKDSKFYLEGEVLFASVGSMVEHYHTHVLPSHQSLLLRHPYGYAGPR EDFE1F11B259646E 3bp2_human SH3 domain-binding protein 2 SH3BP2 3BP2 RES4-23 protein protein human Homo sapiens Human MAAEEMHWPVPMKAIGAQNLLTMPGGVAKAGYLHKKGGTQLQLLKWPLRFVIIHKRCVYYFKSSTSASPQGAFSLSGYNRVMRAAEETTSNNVFPFKIIHISKKHRTWFFSASSEEERKSWMALLRREIGHFHEKKDLPLDTSDSSSDTDSFYGAVERPVDISLSPYPTDNEDYEHDDEDDSYLEPDSPEPGRLEDALMHPPAYPPPPVPTPRKPAFSDMPRAHSFTSKGPGPLLPPPPPKHGLPDVGLAAEDSKRDPLCPRRAEPCPRVPATPRRMSDPPLSTMPTAPGLRKPPCFRESASPSPEPWTPGHGACSTSSAAIMATATSRNCDKLKSFHLSPRGPPTSEPPPVPANKPKFLKIAEEDPPREAAMPGLFVPPVAPRPPALKLPVPEAMARPAVLPRPEKPQLPHLQRSPPDGQSFRSFSFEKPRQPSQADTGGDDSDEDYEKVPLPNSVFVNTTESCEVERLFKATSPRGEPQDGLYCIRNSSTKSGKVLVVWDETSNKVRNYRIFEKDSKFYLEGEVLFVSVGSMVEHYHTHVLPSHQSLLLRHPYGYTGPR 69E6846A4F6D8F15 sh3bp2-tnks2 3 7 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Q06649:p.Arg413Gln mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain R Q Wed May 02 16:54:53 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg413gln' Tue Jun 30 19:12:24 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg413Gln' Q06649:p.Gly418Arg mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain G R Wed May 02 17:41:04 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gly418arg' Tue Jun 30 19:01:18 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly418Arg' Q06649:p.Pro416His mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain P H Wed May 02 19:33:12 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'pro416his' Tue Jun 30 17:00:25 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Pro416His' 4 unspecified role unspecified role prey prey region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Fig. 1E, 2A-D, 3D-E Supp. Fig. 2A, 2B. Wild-Type 3BP2 but not 3BP2 Cherubism mutants Are ribosylated by Tankyrase. Ribosylation induces 3BP2 ubiquitylation and subsequent degradation The Tankyrase binding region, RSPPDG (413-418), was added to the C terminus of the cherubism mutant 3BP2R413Q. Tankyrase was able to bind to the chimeric protein sh3bp2-rnf146 3 5 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 7 unspecified role unspecified role bait bait Q06649:p.Arg413Gln mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain R Q Wed May 02 15:48:35 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg413gln' Tue Jun 30 16:45:34 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg413Gln' region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Fig. 3D, 3E Binding of RNF146 to 3BP2 is ADP-ribosylation dependent. sh3bp2-tnks2-1 3 4 unspecified role unspecified role prey prey region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. 7 unspecified role unspecified role bait bait Q06649:p.Arg413Gln mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain R Q Wed May 02 17:01:21 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg413gln' Tue Jun 30 18:20:52 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg413Gln' region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Q06649:p.Pro416His mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain P H Wed May 02 16:47:03 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'pro416his' Tue Jun 30 18:24:12 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Pro416His' Q06649:p.Gly418Arg mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain G R Wed May 02 17:14:55 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gly418arg' Tue Jun 30 19:23:41 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly418Arg' ecoli Escherichia coli (strain K12) physical association physical association Fig. 1F, 3A, 3C, S3B. proteasome inhibitors LLNL and MG132. sh3bp2-tnks2-2 2 7 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 4 unspecified role unspecified role prey prey region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind range range range range human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. direct interaction direct interaction Supp. Figs. 1 sh3bp2-tnks2-3 2 4 unspecified role unspecified role prey prey region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. 7 unspecified role unspecified role bait bait Q06649:p.Gly423Gln mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain G Q Wed May 02 15:21:21 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gly423gln' Tue Jun 30 18:09:43 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly423Gln' Q06649:p.Arg413His mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain R H Wed May 02 17:01:14 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg413his' Tue Jun 30 17:16:12 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg413His' Q06649:p.Gly418Arg mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain G R Wed May 02 17:09:12 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gly418arg' Tue Jun 30 17:04:58 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly418Arg' hex region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) direct interaction direct interaction Fig. 1G sh3bp2-tnks2-4 1 4 unspecified role unspecified role prey prey region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 8 unspecified role unspecified role bait bait physical association physical association Fig. 3B Intense ubiquitylation of proteins present in the 3BP2 complex was induced when TNKS2 was coexpressed. sh3bp2-tnks-5 3 6 unspecified role unspecified role prey prey region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 7 unspecified role unspecified role bait bait Q06649:p.Arg413Gln mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain R Q Wed May 02 19:41:01 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg413gln' Tue Jun 30 17:52:05 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg413Gln' region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Fig. 1E, S2B 3BP2 ADP-ribosylation Is stimulated by TNKS