UniProt UniProt http://www.uniprot.org Tripartite motif containing protein 27 negatively regulates CD4 T cells by ubiquitinating and inhibiting the class II PI3K-C2β. Tripartite motif containing protein 27 negatively regulates CD4 T cells by ubiquitinating and inhibiting the class II PI3K-C2β. Proc. Natl. Acad. Sci. U.S.A. (0027-8424) 2011 imex curation Cai X., Srivastava S., Sun Y., Li Z., Wu H., Zuvela-Jelaska L., Li J., Salamon R.S., Backer J.M., Skolnik E.Y. Authors have been contacted to verify/confirm origin of some of the used constructs, but did not reply. Origin of PI3KC2β inferred from previous work of J. Domin cited in paper (PI3KC2α and PI3KC2β), see pmid s11533253, 9337861 and 10766823. Edward.Skolnik@nyumc.org 18-Dec-2012: Contacted by IntAct-Help. Human CD4 T-cell cDNA Only protein-protein interactions human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Cai X., Srivastava S., Sun Y., Li Z., Wu H., Zuvela-Jelaska L., Li J., Salamon R.S., Backer J.M., Skolnik E.Y. Authors have been contacted to verify/confirm origin of some of the used constructs, but did not reply. Origin of PI3KC2β inferred from previous work of J. Domin cited in paper (PI3KC2α and PI3KC2β), see pmid s11533253, 9337861 and 10766823. Edward.Skolnik@nyumc.org Accepted 2012-NOV-22 by ORCHARD 18-Dec-2012: Contacted by IntAct-Help. Human CD4 T-cell cDNA Proc. Natl. Acad. Sci. U.S.A. (0027-8424) 2011 Only protein-protein interactions imex curation Tripartite motif containing protein 27 negatively regulates CD4 T cells by ubiquitinating and inhibiting the class II PI3K-C2β. Tripartite motif containing protein 27 negatively regulates CD4 T cells by ubiquitinating and inhibiting the class II PI3K-C2β. Proc. Natl. Acad. Sci. U.S.A. (0027-8424) 2011 imex curation Cai X., Srivastava S., Sun Y., Li Z., Wu H., Zuvela-Jelaska L., Li J., Salamon R.S., Backer J.M., Skolnik E.Y. Authors have been contacted to verify/confirm origin of some of the used constructs, but did not reply. Origin of PI3KC2β inferred from previous work of J. Domin cited in paper (PI3KC2α and PI3KC2β), see pmid s11533253, 9337861 and 10766823. Edward.Skolnik@nyumc.org 18-Dec-2012: Contacted by IntAct-Help. Human CD4 T-cell cDNA Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Cai X., Srivastava S., Sun Y., Li Z., Wu H., Zuvela-Jelaska L., Li J., Salamon R.S., Backer J.M., Skolnik E.Y. Authors have been contacted to verify/confirm origin of some of the used constructs, but did not reply. Origin of PI3KC2β inferred from previous work of J. Domin cited in paper (PI3KC2α and PI3KC2β), see pmid s11533253, 9337861 and 10766823. Edward.Skolnik@nyumc.org Accepted 2012-NOV-22 by ORCHARD 18-Dec-2012: Contacted by IntAct-Help. Human CD4 T-cell cDNA Proc. Natl. Acad. Sci. U.S.A. (0027-8424) 2011 Only protein-protein interactions imex curation Tripartite motif containing protein 27 negatively regulates CD4 T cells by ubiquitinating and inhibiting the class II PI3K-C2β. Tripartite motif containing protein 27 negatively regulates CD4 T cells by ubiquitinating and inhibiting the class II PI3K-C2β. Proc. Natl. Acad. Sci. U.S.A. (0027-8424) 2011 imex curation Cai X., Srivastava S., Sun Y., Li Z., Wu H., Zuvela-Jelaska L., Li J., Salamon R.S., Backer J.M., Skolnik E.Y. Authors have been contacted to verify/confirm origin of some of the used constructs, but did not reply. Origin of PI3KC2β inferred from previous work of J. Domin cited in paper (PI3KC2α and PI3KC2β), see pmid s11533253, 9337861 and 10766823. Edward.Skolnik@nyumc.org 18-Dec-2012: Contacted by IntAct-Help. Human CD4 T-cell cDNA Only protein-protein interactions chlae Chlorocebus aethiops Cercopithecus aethiops Grivet Green monkey fluorescence imaging fluorescence microscopy fluorescence imaging anti tag immunost anti tag immunostaining anti tag immunost Cai X., Srivastava S., Sun Y., Li Z., Wu H., Zuvela-Jelaska L., Li J., Salamon R.S., Backer J.M., Skolnik E.Y. Authors have been contacted to verify/confirm origin of some of the used constructs, but did not reply. Origin of PI3KC2β inferred from previous work of J. Domin cited in paper (PI3KC2α and PI3KC2β), see pmid s11533253, 9337861 and 10766823. Edward.Skolnik@nyumc.org Accepted 2012-NOV-22 by ORCHARD 18-Dec-2012: Contacted by IntAct-Help. Human CD4 T-cell cDNA Proc. Natl. Acad. Sci. U.S.A. (0027-8424) 2011 Only protein-protein interactions imex curation Tripartite motif containing protein 27 negatively regulates CD4 T cells by ubiquitinating and inhibiting the class II PI3K-C2β. Tripartite motif containing protein 27 negatively regulates CD4 T cells by ubiquitinating and inhibiting the class II PI3K-C2β. Proc. Natl. Acad. Sci. U.S.A. (0027-8424) 2011 imex curation Cai X., Srivastava S., Sun Y., Li Z., Wu H., Zuvela-Jelaska L., Li J., Salamon R.S., Backer J.M., Skolnik E.Y. Authors have been contacted to verify/confirm origin of some of the used constructs, but did not reply. Origin of PI3KC2β inferred from previous work of J. Domin cited in paper (PI3KC2α and PI3KC2β), see pmid s11533253, 9337861 and 10766823. Edward.Skolnik@nyumc.org 18-Dec-2012: Contacted by IntAct-Help. Human CD4 T-cell cDNA Only protein-protein interactions human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Cai X., Srivastava S., Sun Y., Li Z., Wu H., Zuvela-Jelaska L., Li J., Salamon R.S., Backer J.M., Skolnik E.Y. Authors have been contacted to verify/confirm origin of some of the used constructs, but did not reply. Origin of PI3KC2β inferred from previous work of J. Domin cited in paper (PI3KC2α and PI3KC2β), see pmid s11533253, 9337861 and 10766823. Edward.Skolnik@nyumc.org Accepted 2012-NOV-22 by ORCHARD 18-Dec-2012: Contacted by IntAct-Help. Human CD4 T-cell cDNA Proc. Natl. Acad. Sci. U.S.A. (0027-8424) 2011 Only protein-protein interactions imex curation p3c2b_human Phosphatidylinositol 4-phosphate 3-kinase C2 domain-containing subunit beta Phosphoinositide 3-kinase-C2-beta C2-PI3K PIK3C2B protein protein human Homo sapiens Human MSSTQGNGEHWKSLESVGISRKELAMAEALQMEYDALSRLRHDKEENRAKQNADPSLISWDEPGVDFYSKPAGRRTDLKLLRGLSGSDPTLNYNSLSPQEGPPNHSTSQGPQPGSDPWPKGSLSGDYLYIFDGSDGGVSSSPGPGDIEGSCKKLSPPPLPPRASIWDTPPLPPRKGSPSSSKISQPSDINTFSLVEQLPGKLLEHRILEEEEVLGGGGQGRLLGSVDYDGINDAITRLNLKSTYDAEMLRDATRGWKEGRGPLDFSKDTSGKPVARSKTMPPQVPPRTYASRYGNRKNATPGKNRRISAAPVGSRPHTVANGHELFEVSEERDEEVAAFCHMLDILRSGSDIQDYFLTGYVWSAVTPSPEHLGDEVNLKVTVLCDRLQEALTFTCNCSSTVDLLIYQTLCYTHDDLRNVDVGDFVLKPCGLEEFLQNKHALGSHEYIQYCRKFDIDIRLQLMEQKVVRSDLARTVNDDQSPSTLNYLVHLQERPVKQTISRQALSLLFDTYHNEVDAFLLADGDFPLKADRVVQSVKAICNALAAVETPEITSALNQLPPCPSRMQPKIQKDPSVLAVRENREKVVEALTAAILDLVELYCNTFNADFQTAVPGSRKHDLVQEACHFARSLAFTVYATHRIPIIWATSYEDFYLSCSLSHGGKELCSPLQTRRAHFSKYLFHLIVWDQQICFPVQVNRLPRETLLCATLYALPIPPPGSSSEANKQRRVPEALGWVTTPLFNFRQVLTCGRKLLGLWPATQENPSARWSAPNFHQPDSVILQIDFPTSAFDIKFTSPPGDKFSPRYEFGSLREEDQRKLKDIMQKESLYWLTDADKKRLWEKRYYCHSEVSSLPLVLASAPSWEWACLPDIYVLLKQWTHMNHQDALGLLHATFPDQEVRRMAVQWIGSLSDAELLDYLPQLVQALKYECYLDSPLVRFLLKRAVSDLRVTHYFFWLLKDGLKDSQFSIRYQYLLAALLCCCGKGLREEFNRQCWLVNALAKLAQQVREAAPSARQGILRTGLEEVKQFFALNGSCRLPLSPSLLVKGIVPRDCSYFNSNAVPLKLSFQNVDPLGENIRVIFKCGDDLRQDMLTLQMIRIMSKIWVQEGLDMRMVIFRCFSTGRGRGMVEMIPNAETLRKIQVEHGVTGSFKDRPLADWLQKHNPGEDEYEKAVENFIYSCAGCCVATYVLGICDRHNDNIMLKTTGHMFHIDFGRFLGHAQMFGNIKRDRAPFVFTSDMAYVINGGDKPSSRFHDFVDLCCQAYNLIRKHTHLFLNLLGLMLSCGIPELSDLEDLKYVYDALRPQDTEANATTYFTRLIESSLGSVATKLNFFIHNLAQMKFTGSDDRLTLSFASRTHTLKSSGRISDVFLCRHEKIFHPNKGYIYVVKVMRENTHEATYIQRTFEEFQELHNKLRLLFPSSHLPSFPSRFVIGRSRGEAVAERRREELNGYIWHLIHAPPEVAECDLVYTFFHPLPRDEKAMGTSPAPKSSDGTWARPVGKVGGEVKLSISYKNNKLFIMVMHIRGLQLLQDGNDPDPYVKIYLLPDPQKTTKRKTKVARKTCNPTYNEMLVYDGIPKGDLQQRELQLSVLSEQGFWENVLLGEVNIRLRELDLAQEKTGWFALGSRSHGTL CA817BA77C12E833 tri27_human Zinc finger protein RFP TRIM27 RNF76 RFP Ret finger protein Tripartite motif-containing protein 27 RING finger protein 76 RING-type E3 ubiquitin transferase TRIM27 protein protein human Homo sapiens Human MASGSVAECLQQETTCPVCLQYFAEPMMLDCGHNICCACLARCWGTAETNVSCPQCRETFPQRHMRPNRHLANVTQLVKQLRTERPSGPGGEMGVCEKHREPLKLYCEEDQMPICVVCDRSREHRGHSVLPLEEAVEGFKEQIQNQLDHLKRVKDLKKRRRAQGEQARAELLSLTQMEREKIVWEFEQLYHSLKEHEYRLLARLEELDLAIYNSINGAITQFSCNISHLSSLIAQLEEKQQQPTRELLQDIGDTLSRAERIRIPEPWITPPDLQEKIHIFAQKCLFLTESLKQFTEKMQSDMEKIQELREAQLYSVDVTLDPDTAYPSLILSDNLRQVRYSYLQQDLPDNPERFNLFPCVLGSPCFIAGRHYWEVEVGDKAKWTIGVCEDSVCRKGGVTSAPQNGFWAVSLWYGKEYWALTSPMTALPLRTPLQRVGIFLDYDAGEVSFYNVTERCHTFTFSHATFCGPVRPYFSLSYSGGKSAAPLIICPMSGIDGFSGHVGNHGHSMETSP 6F963D9048D8A731 pik3c2b-trim27 2 6 unspecified role unspecified role prey prey 5 unspecified role unspecified role bait bait physical association physical association no figure pik3c2b-trim27-1 1 6 unspecified role unspecified role prey prey region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 5 unspecified role unspecified role bait bait region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association 1A pik3c2b-trim27-2 1 6 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 5 unspecified role unspecified role prey prey region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association 1A pik3c2b-trim27-3 4 6 unspecified role unspecified role prey prey 5 unspecified role unspecified role bait bait physical association physical association 1C pik3c2b-trim27-4 3 6 unspecified role unspecified role neutral component neutral component region mcherry mcherry fluorescent protein tag mcherry undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 5 unspecified role unspecified role neutral component neutral component region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined colocalization colocalization 1B