MINT MINT, Dpt of Biology, University of Rome Tor Vergata MINT http://mint.bio.uniroma2.it/mint url>http://mint.bio.uniroma2.it/mint/ https://mint.bio.uniroma2.it MINT-[0-9]+ https://mint.bio.uniroma2.it/index.php/results-interactions/?id=${ac} The Molecular INTeraction database (MINT) is a relational database designed to store interactions between biological molecules. http://mint.bio.uniroma2.it PABP is not essential for microRNA-mediated translational repression and deadenylation in vitro. PABP is not essential for microRNA-mediated translational repression and deadenylation in vitro. EMBO J. (0261-4189) 2011 imex curation Fukaya T., Tomari Y. Only protein-protein interactions drome-schneider_2 Drosophila melanogaster late embryo cells Fruit fly schneider_2 Established from the late embryo of Drosophila melanogaster Mixture of diploid and tetraploid. Originally had some XY cells now predominantly XX. anti tag coip anti tag coimmunoprecipitation anti tag coip predetermined predetermined participant predetermined Fukaya T., Tomari Y. EMBO J. (0261-4189) 2011 Only protein-protein interactions imex curation pabp_drome Polyadenylate-binding protein pAbp CG5119 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MASLYVGDLPQDVNESGLFDKFSSAGPVLSIRVCRDVITRRSLGYAYVNFQQPADAERALDTMNFDLVRNKPIRIMWSQRDPSLRRSGVGNVFIKNLDRAIDNKAIYDTFSAFGNILSCKVATDEKGNSKGYGFVHFETEEAANTSIDKVNGMLLNGKKVYVGKFIPRKEREKELGEKAKLFTNVYVKNFTEDFDDEKLKEFFEPYGKITSYKVMSKEDGKSKGFGFVAFETTEAAEAAVQALNGKDMGEGKSLYVARAQKKAERQQELKRKFEELKQKRHESVFGVNLYVKNLDDTIDDDRLRIAFSPYGNITSAKVMTDEEGRSKGFGFVCFNAASEATCAVTELNGRVVGSKPLYVALAQRKEERKAHLASQYMRHMTGMRMQQLGQIYQPNAASGFFVPTLPSNQRFFGSQVATQMRNTPRWVPQVRPPAAIQGVQAGAAAAGGFQGTAGAVPTQFRSAAAGARGAQPQVQGTHAAAAAANNMRNTGARAITGQQTAAPNMQIPGAQIAGGAQQRTSNYKYTSNMRNPPVPQLHQTQPIPQQLQGKNSEKLIASLLANAKPQEQKQILGERLYPMIEHMHANLAGKITGMLLEIENSELLHMIEDQEALKAKVEEAVAVLQVHRVTEPAN DIP protein P21187 original sequence version: 105 DIP protein P21187 original sequence version: 146 A76B204ED206392E gawky_drome Protein Gawky gw GW182 CG31992 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MREALFSQDGWGCQHVNQDTNWEVPSSPEPANKDAPGPPMWKPSINNGTDLWESNLRNGGQPAAQQVPKPSWGHTPSSNLGGTWGEDDDGADSSSVWTGGAVSNAGSGAAVGVNQAGVNVGPGGVVSSGGPQWGQGVVGVGLGSTGGNGSSNITGSSGVATGSSGNSSNAGNGWGDPREIRPLGVGGSMDIRNVEHRGGNGSGATSSDPRDIRMIDPRDPIRGDPRGISGRLNGTSEMWGHHPQMSHNQLQGINKMVGQSVATASTSVGTSGSGIGPGGPGPSTVSGNIPTQWGPAQPVSVGVSGPKDMSKQISGWEEPSPPPQRRSIPNYDDGTSLWGQQTRVPAASGHWKDMTDSIGRSSHLMRGQSQTGGIGIAGVGNSNVPVGANPSNPISSVVGPQARIPSVGGVQHKPDGGAMWVHSGNVGGRNNVAAVTTWGDDTHSVNVGAPSSGSVSSNNWVDDKSNSTLAQNSWSDPAPVGVSWGNKQSKPPSNSASSGWSTAAGVVDGVDLGSEWNTHGGIIGKSQQQQKLAGLNVGMVNVINAEIIKQSKQYRILVENGFKKEDVERALVIANMNIEEAADMLRANSSLSMDGWRRHDESLGSYADHNSSTSSGGFAGRYPVNSGQPSMSFPHNNLMNNMGGTAVTGGNNNTNMTALQVQKYLNQGQHGVAVGPQAVGNSSAVSVGFGQNTSNAAVAGAASVNIAANTNNQPSGQQIRMLGQQIQLAIHSGFISSQILTQPLTQTTLNLLNQLLSNIKHLQAAQQSLTRGGNVNPMAVNVAISKYKQQIQNLQNQINAQQAVYVKQQNMQPTSQQQQPQQQQLPSVHLSNSGNDYLRGHDAINNLQSNFSELNINKPSGYQGASNQQSRLNQWKLPVLDKEINSDSTEFSRAPGATKQNLTANTSNINSLGLQNDSTWSTGRSIGDGWPDPSSDNENKDWSVAQPTSAATAYTDLVQEFEPGKPWKGSQIKSIEDDPSITPGSVARSPLSINSTPKDADIFANTGKNSPTDLPPLSLSSSTWSFNPNQNYPSHSWSDNSQQCTATSELWTSPLNKSSSRGPPPGLTANSNKSANSNASTPTTITGGANGWLQPRSGGVQTTNTNWTGGNTTWGSSWLLLKNLTAQIDGPTLRTLCMQHGPLVSFHPYLNQGIALCKYTTREEANKAQMALNNCVLANTTIFAESPSENEVQSIMQHLPQTPSSTSSSGTSGGNVGGVGTSANNANSGSAACLSGNNSGNGNGSASGAGSGNNGNSSCNNSAAGGGSSSNNTITTVANSNLVGSSGSVSNSSGVTANSSTVSVVSCTASGNSINGAGTANSSGSKSSANNLASGQSSASNLTNSTNSTWRQTSQNQALQSQSRPSGREADFDYISLVYSIVDD DIP protein Q8SY33 original sequence version: 96 4E87DFE49050883E q7ky08_drome AGO1 dAGO1 AGO1-RB MRE20 ago1 ago1-1 anon-WO0257455.29 argonaute1 dAgo1 l(2)04845 l(2)4845 l(2)k00208 l(2)k08121 CG6671 Dmel_CG6671 AGO-1 Ago Ago-1 Ago1 Agp1 Dm Ago1 DmAgo1 Dmel\CG6671 dmAgo1 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MYPVGQQSQWTPSPTRPQSPSQAQTSFDTLTSPPAPGSSVNPTAVTSPSAQNVAAGGATVAGAAATAAQVASALGATTGSVTPAIATATPATQPDMPVFTCPRRPNLGREGRPIVLRANHFQVTMPRGYVHHYDINIQPDKCPRKVNREIIETMVHAYSKIFGVLKPVFDGRNNLYTRDPLPIGNERLELEVTLPGEGKDRIFRVTIKWQAQVSLFNLEEALEGRTRQIPYDAILALDVVMRHLPSMTYTPVGRSFFSSPEGYYHPLGGGREVWFGFHQSVRPSQWKMMLNIDVSATAFYKAQPVIDFMCEVLDIRDINEQRKPLTDSQRVKFTKEIKGLKIEITHCGQMRRKYRVCNVTRRPAQMQSFPLQLENGQTVECTVAKYFLDKYRMKLRYPHLPCLQVGQEHKHTYLPLEVCNIVAGQRCIKKLTDMQTSTMIKATARSAPDREREINNLVKRADFNNDSYVQEFGLTISNSMMEVRGRVLPPPKLQYGGRVSTGLTGQQLFPPQNKVSLASPNQGVWDMRGKQFFTGVEIRIWAIACFAPQRTVREDALRNFTQQLQKISNDAGMPIIGQPCFCKYATGPDQVEPMFRYLKITFPGLQLVVVVLPGKTPVYAEVKRVGDTVLGMATQCVQAKNVNKTSPQTLSNLCLKINVKLGGINSILVPSIRPKVFNEPVIFLGADVTHPPAGDNKKPSIAAVVGSMDAHPSRYAATVRVQQHRQEIIQELSSMVRELLIMFYKSTGGYKPHRIILYRDGVSEGQFPHVLQHELTAIREACIKLEPEYRPGITFIVVQKRHHTRLFCAEKKEQSGKSGNIPAGTTVDVGITHPTEFDFYLCSHQGIQGTSRPSHYHVLWDDNHFDSDELQCLTYQLCHTYVRCTRSVSIPAPAYYAHLVAFRARYHLVEKEHDSGEGSHQSGCSEDRTPGAMARAITVHADTKKVMYFA DIP protein Q7KY08 original sequence version: 41 mint A7684595164C267E gw-ago1 1 Ago1 4 western blot western blot Immuno blot Immuno blot predetermined predetermined participant predetermined unspecified role unspecified role prey prey cell lysate cell lysate physiological level physiological level endogenous level endogenous endogenous endogenous level PABP 2 western blot western blot Immuno blot Immuno blot predetermined predetermined participant predetermined unspecified role unspecified role prey prey cell lysate cell lysate physiological level physiological level endogenous level endogenous endogenous endogenous level GW182 3 anti tag western anti tag western blot anti tag western predetermined predetermined participant predetermined unspecified role unspecified role bait bait over-expressed over expressed level over-expressed cell lysate cell lysate ha tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined association association f2c mint In addition, interaction between GW182 and PABP was strongly inhibited by GST PAIP2 (Figure 2C), consistent with the fact that both PAIP2 and GW182 have a PAM2 motif.