IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ IL-1 receptor accessory protein-like 1 associated with mental retardation and autism mediates synapse formation by trans-synaptic interaction with protein tyrosine phosphatase δ. IL-1 receptor accessory protein-like 1 associated with mental retardation and autism mediates synapse formation by trans-synaptic interaction with protein tyrosine phosphatase δ. J. Neurosci. (0270-6474) 2011 imex curation Yoshida T., Yasumura M., Uemura T., Lee S.J., Ra M., Taguchi R., Iwakura Y., Mishina M. Synapse - Interactions of proteins with an established role in the presynapse. I have added an InterPro ref for ptprd_mouse -phosphatase subunit at Feature level - hope this is right place. DATE 8/3/12:- Wei-Muns reply to my isoform chasing email:- "Sorry for the delay - I'll do a quick update of the entry for the isoforms requested and merge only the isoforms as a priority." I'll send the update next week - however since the changes have to be integrated at SIB it may take the changes a little while before they are reflected in the database - but it should be in the next 2-3 weeks. " As a result, I do not anticipate any quick resolution to this. Fig 5 B/C is the problem - isoforms used are not in UniProt and I cannot create new proteins as I dont know the full sequence of the proteins. I have only used G3X9S7 in Figs 5B/C and Fig 3 for Mass Spec as in these instances it is clear the MeA/B peptides have been detected. Otherwise (unless I have missed something) no particular isoform has been detected - so Reviewed entry used, as at least the domain locations can be guessed at. Written 7/2/12:-(there may have been updates in Ensembl/UniProt since) From Fig 5A - Trembl entry G3X9S7 is currently the best entry for protein Ptprd/PTP-Delta -1912 aas and longest transcript, and includes MeA and MeB sequences given in fig 5A. ( The only Reviewed UniProt entry does NOT, and is taken from a shorter protein- entry added ~year 1993/5). Ensembl do not seem to have caught up with G3X9S7 yet -( as G3X9S7 entry only created 11/2011) - it is not stated in any Ensembl External Refs for the longest 1912 aa transcript given - though this is the same protein from blasting Ensembl ENSMUSP00000102910/ENSMUST00000107289 Mouse IL1RAPL1-ECD-Fc was immobilised on Protein A-Sepharose beads. mishina@m.u-tokyo.ac.jp Only protein-protein interactions in vitro In vitro confocal microscopy confocal microscopy immunostaining immunostaining Yoshida T., Yasumura M., Uemura T., Lee S.J., Ra M., Taguchi R., Iwakura Y., Mishina M. Synapse - Interactions of proteins with an established role in the presynapse. I have added an InterPro ref for ptprd_mouse -phosphatase subunit at Feature level - hope this is right place. I have added an InterPro ref for ptprd_mouse -phosphatase subunit at Feature level - hope this is right place. Mouse IL1RAPL1-ECD-Fc was immobilised on Protein A-Sepharose beads. mishina@m.u-tokyo.ac.jp Accepted 2012-JUN-18 by ORCHARD J. Neurosci. (0270-6474) 2011 Only protein-protein interactions imex curation date:28/05/12 The entry G3X9S7 has now been incorporated into the reviwed entry Q64487 (PTPRD_MOUSE) - though I was not notified of this! Canonical sequence is now Q64487-4. I have altered sequence info for features for ALL Expts inc accepted ones. IL-1 receptor accessory protein-like 1 associated with mental retardation and autism mediates synapse formation by trans-synaptic interaction with protein tyrosine phosphatase δ. IL-1 receptor accessory protein-like 1 associated with mental retardation and autism mediates synapse formation by trans-synaptic interaction with protein tyrosine phosphatase δ. J. Neurosci. (0270-6474) 2011 imex curation Yoshida T., Yasumura M., Uemura T., Lee S.J., Ra M., Taguchi R., Iwakura Y., Mishina M. Synapse - Interactions of proteins with an established role in the presynapse. I have added an InterPro ref for ptprd_mouse -phosphatase subunit at Feature level - hope this is right place. DATE 8/3/12:- Wei-Muns reply to my isoform chasing email:- "Sorry for the delay - I'll do a quick update of the entry for the isoforms requested and merge only the isoforms as a priority." I'll send the update next week - however since the changes have to be integrated at SIB it may take the changes a little while before they are reflected in the database - but it should be in the next 2-3 weeks. " As a result, I do not anticipate any quick resolution to this. Fig 5 B/C is the problem - isoforms used are not in UniProt and I cannot create new proteins as I dont know the full sequence of the proteins. I have only used G3X9S7 in Figs 5B/C and Fig 3 for Mass Spec as in these instances it is clear the MeA/B peptides have been detected. Otherwise (unless I have missed something) no particular isoform has been detected - so Reviewed entry used, as at least the domain locations can be guessed at. Written 7/2/12:-(there may have been updates in Ensembl/UniProt since) From Fig 5A - Trembl entry G3X9S7 is currently the best entry for protein Ptprd/PTP-Delta -1912 aas and longest transcript, and includes MeA and MeB sequences given in fig 5A. ( The only Reviewed UniProt entry does NOT, and is taken from a shorter protein- entry added ~year 1993/5). Ensembl do not seem to have caught up with G3X9S7 yet -( as G3X9S7 entry only created 11/2011) - it is not stated in any Ensembl External Refs for the longest 1912 aa transcript given - though this is the same protein from blasting Ensembl ENSMUSP00000102910/ENSMUST00000107289 Mouse IL1RAPL1-ECD-Fc was immobilised on Protein A-Sepharose beads. mishina@m.u-tokyo.ac.jp Only protein-protein interactions in vitro In vitro spr surface plasmon resonance Optical biosensor BIAcore(r) BIAcore(r) Optical biosensor spr predetermined predetermined participant predetermined Yoshida T., Yasumura M., Uemura T., Lee S.J., Ra M., Taguchi R., Iwakura Y., Mishina M. Synapse - Interactions of proteins with an established role in the presynapse. I have added an InterPro ref for ptprd_mouse -phosphatase subunit at Feature level - hope this is right place. Mouse IL1RAPL1-ECD-Fc was immobilised on Protein A-Sepharose beads. mishina@m.u-tokyo.ac.jp Accepted 2012-JUN-18 by ORCHARD J. Neurosci. (0270-6474) 2011 Only protein-protein interactions imex curation date:28/05/12 The entry G3X9S7 has now been incorporated into the reviwed entry Q64487 (PTPRD_MOUSE) - though I was not notified of this! Canonical sequence is now Q64487-4. I have altered sequence info for features for ALL Expts inc accepted ones. IL-1 receptor accessory protein-like 1 associated with mental retardation and autism mediates synapse formation by trans-synaptic interaction with protein tyrosine phosphatase δ. IL-1 receptor accessory protein-like 1 associated with mental retardation and autism mediates synapse formation by trans-synaptic interaction with protein tyrosine phosphatase δ. J. Neurosci. (0270-6474) 2011 imex curation Yoshida T., Yasumura M., Uemura T., Lee S.J., Ra M., Taguchi R., Iwakura Y., Mishina M. Synapse - Interactions of proteins with an established role in the presynapse. I have added an InterPro ref for ptprd_mouse -phosphatase subunit at Feature level - hope this is right place. DATE 8/3/12:- Wei-Muns reply to my isoform chasing email:- "Sorry for the delay - I'll do a quick update of the entry for the isoforms requested and merge only the isoforms as a priority." I'll send the update next week - however since the changes have to be integrated at SIB it may take the changes a little while before they are reflected in the database - but it should be in the next 2-3 weeks. " As a result, I do not anticipate any quick resolution to this. Fig 5 B/C is the problem - isoforms used are not in UniProt and I cannot create new proteins as I dont know the full sequence of the proteins. I have only used G3X9S7 in Figs 5B/C and Fig 3 for Mass Spec as in these instances it is clear the MeA/B peptides have been detected. Otherwise (unless I have missed something) no particular isoform has been detected - so Reviewed entry used, as at least the domain locations can be guessed at. Written 7/2/12:-(there may have been updates in Ensembl/UniProt since) From Fig 5A - Trembl entry G3X9S7 is currently the best entry for protein Ptprd/PTP-Delta -1912 aas and longest transcript, and includes MeA and MeB sequences given in fig 5A. ( The only Reviewed UniProt entry does NOT, and is taken from a shorter protein- entry added ~year 1993/5). Ensembl do not seem to have caught up with G3X9S7 yet -( as G3X9S7 entry only created 11/2011) - it is not stated in any Ensembl External Refs for the longest 1912 aa transcript given - though this is the same protein from blasting Ensembl ENSMUSP00000102910/ENSMUST00000107289 Mouse IL1RAPL1-ECD-Fc was immobilised on Protein A-Sepharose beads. mishina@m.u-tokyo.ac.jp Only protein-protein interactions mouse-brain Mus musculus brain brain Brain [cerebrum] pull down pull down western blot western blot Immuno blot Immuno blot Yoshida T., Yasumura M., Uemura T., Lee S.J., Ra M., Taguchi R., Iwakura Y., Mishina M. Synapse - Interactions of proteins with an established role in the presynapse. I have added an InterPro ref for ptprd_mouse -phosphatase subunit at Feature level - hope this is right place. Mouse IL1RAPL1-ECD-Fc was immobilised on Protein A-Sepharose beads. mishina@m.u-tokyo.ac.jp Accepted 2012-JUN-18 by ORCHARD J. Neurosci. (0270-6474) 2011 Only protein-protein interactions imex curation date:28/05/12 The entry G3X9S7 has now been incorporated into the reviwed entry Q64487 (PTPRD_MOUSE) - though I was not notified of this! Canonical sequence is now Q64487-4. I have altered sequence info for features for ALL Expts inc accepted ones. IL-1 receptor accessory protein-like 1 associated with mental retardation and autism mediates synapse formation by trans-synaptic interaction with protein tyrosine phosphatase δ. IL-1 receptor accessory protein-like 1 associated with mental retardation and autism mediates synapse formation by trans-synaptic interaction with protein tyrosine phosphatase δ. J. Neurosci. (0270-6474) 2011 imex curation Yoshida T., Yasumura M., Uemura T., Lee S.J., Ra M., Taguchi R., Iwakura Y., Mishina M. Synapse - Interactions of proteins with an established role in the presynapse. I have added an InterPro ref for ptprd_mouse -phosphatase subunit at Feature level - hope this is right place. DATE 8/3/12:- Wei-Muns reply to my isoform chasing email:- "Sorry for the delay - I'll do a quick update of the entry for the isoforms requested and merge only the isoforms as a priority." I'll send the update next week - however since the changes have to be integrated at SIB it may take the changes a little while before they are reflected in the database - but it should be in the next 2-3 weeks. " As a result, I do not anticipate any quick resolution to this. Fig 5 B/C is the problem - isoforms used are not in UniProt and I cannot create new proteins as I dont know the full sequence of the proteins. I have only used G3X9S7 in Figs 5B/C and Fig 3 for Mass Spec as in these instances it is clear the MeA/B peptides have been detected. Otherwise (unless I have missed something) no particular isoform has been detected - so Reviewed entry used, as at least the domain locations can be guessed at. Written 7/2/12:-(there may have been updates in Ensembl/UniProt since) From Fig 5A - Trembl entry G3X9S7 is currently the best entry for protein Ptprd/PTP-Delta -1912 aas and longest transcript, and includes MeA and MeB sequences given in fig 5A. ( The only Reviewed UniProt entry does NOT, and is taken from a shorter protein- entry added ~year 1993/5). Ensembl do not seem to have caught up with G3X9S7 yet -( as G3X9S7 entry only created 11/2011) - it is not stated in any Ensembl External Refs for the longest 1912 aa transcript given - though this is the same protein from blasting Ensembl ENSMUSP00000102910/ENSMUST00000107289 Mouse IL1RAPL1-ECD-Fc was immobilised on Protein A-Sepharose beads. mishina@m.u-tokyo.ac.jp Only protein-protein interactions mouse-brain Mus musculus brain brain Brain [cerebrum] pull down pull down sequence tag sequence tag identification sequence tag Yoshida T., Yasumura M., Uemura T., Lee S.J., Ra M., Taguchi R., Iwakura Y., Mishina M. Synapse - Interactions of proteins with an established role in the presynapse. I have added an InterPro ref for ptprd_mouse -phosphatase subunit at Feature level - hope this is right place. Mouse IL1RAPL1-ECD-Fc was immobilised on Protein A-Sepharose beads. mishina@m.u-tokyo.ac.jp Accepted 2012-JUN-18 by ORCHARD J. Neurosci. (0270-6474) 2011 Only protein-protein interactions imex curation date:28/05/12 The entry G3X9S7 has now been incorporated into the reviwed entry Q64487 (PTPRD_MOUSE) - though I was not notified of this! Canonical sequence is now Q64487-4. I have altered sequence info for features for ALL Expts inc accepted ones. IL-1 receptor accessory protein-like 1 associated with mental retardation and autism mediates synapse formation by trans-synaptic interaction with protein tyrosine phosphatase δ. IL-1 receptor accessory protein-like 1 associated with mental retardation and autism mediates synapse formation by trans-synaptic interaction with protein tyrosine phosphatase δ. J. Neurosci. (0270-6474) 2011 imex curation Yoshida T., Yasumura M., Uemura T., Lee S.J., Ra M., Taguchi R., Iwakura Y., Mishina M. Synapse - Interactions of proteins with an established role in the presynapse. I have added an InterPro ref for ptprd_mouse -phosphatase subunit at Feature level - hope this is right place. DATE 8/3/12:- Wei-Muns reply to my isoform chasing email:- "Sorry for the delay - I'll do a quick update of the entry for the isoforms requested and merge only the isoforms as a priority." I'll send the update next week - however since the changes have to be integrated at SIB it may take the changes a little while before they are reflected in the database - but it should be in the next 2-3 weeks. " As a result, I do not anticipate any quick resolution to this. Fig 5 B/C is the problem - isoforms used are not in UniProt and I cannot create new proteins as I dont know the full sequence of the proteins. I have only used G3X9S7 in Figs 5B/C and Fig 3 for Mass Spec as in these instances it is clear the MeA/B peptides have been detected. Otherwise (unless I have missed something) no particular isoform has been detected - so Reviewed entry used, as at least the domain locations can be guessed at. Written 7/2/12:-(there may have been updates in Ensembl/UniProt since) From Fig 5A - Trembl entry G3X9S7 is currently the best entry for protein Ptprd/PTP-Delta -1912 aas and longest transcript, and includes MeA and MeB sequences given in fig 5A. ( The only Reviewed UniProt entry does NOT, and is taken from a shorter protein- entry added ~year 1993/5). Ensembl do not seem to have caught up with G3X9S7 yet -( as G3X9S7 entry only created 11/2011) - it is not stated in any Ensembl External Refs for the longest 1912 aa transcript given - though this is the same protein from blasting Ensembl ENSMUSP00000102910/ENSMUST00000107289 Mouse IL1RAPL1-ECD-Fc was immobilised on Protein A-Sepharose beads. mishina@m.u-tokyo.ac.jp Only protein-protein interactions mouse-neuron Mus musculus neuronal cell neuron Neuron [Neuronal cell; Neural cell anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Yoshida T., Yasumura M., Uemura T., Lee S.J., Ra M., Taguchi R., Iwakura Y., Mishina M. Synapse - Interactions of proteins with an established role in the presynapse. I have added an InterPro ref for ptprd_mouse -phosphatase subunit at Feature level - hope this is right place. Mouse IL1RAPL1-ECD-Fc was immobilised on Protein A-Sepharose beads. mishina@m.u-tokyo.ac.jp Accepted 2012-JUN-18 by ORCHARD J. Neurosci. (0270-6474) 2011 Only protein-protein interactions imex curation date:28/05/12 The entry G3X9S7 has now been incorporated into the reviwed entry Q64487 (PTPRD_MOUSE) - though I was not notified of this! Canonical sequence is now Q64487-4. I have altered sequence info for features for ALL Expts inc accepted ones. IL-1 receptor accessory protein-like 1 associated with mental retardation and autism mediates synapse formation by trans-synaptic interaction with protein tyrosine phosphatase δ. IL-1 receptor accessory protein-like 1 associated with mental retardation and autism mediates synapse formation by trans-synaptic interaction with protein tyrosine phosphatase δ. J. Neurosci. (0270-6474) 2011 imex curation Yoshida T., Yasumura M., Uemura T., Lee S.J., Ra M., Taguchi R., Iwakura Y., Mishina M. Synapse - Interactions of proteins with an established role in the presynapse. I have added an InterPro ref for ptprd_mouse -phosphatase subunit at Feature level - hope this is right place. DATE 8/3/12:- Wei-Muns reply to my isoform chasing email:- "Sorry for the delay - I'll do a quick update of the entry for the isoforms requested and merge only the isoforms as a priority." I'll send the update next week - however since the changes have to be integrated at SIB it may take the changes a little while before they are reflected in the database - but it should be in the next 2-3 weeks. " As a result, I do not anticipate any quick resolution to this. Fig 5 B/C is the problem - isoforms used are not in UniProt and I cannot create new proteins as I dont know the full sequence of the proteins. I have only used G3X9S7 in Figs 5B/C and Fig 3 for Mass Spec as in these instances it is clear the MeA/B peptides have been detected. Otherwise (unless I have missed something) no particular isoform has been detected - so Reviewed entry used, as at least the domain locations can be guessed at. Written 7/2/12:-(there may have been updates in Ensembl/UniProt since) From Fig 5A - Trembl entry G3X9S7 is currently the best entry for protein Ptprd/PTP-Delta -1912 aas and longest transcript, and includes MeA and MeB sequences given in fig 5A. ( The only Reviewed UniProt entry does NOT, and is taken from a shorter protein- entry added ~year 1993/5). Ensembl do not seem to have caught up with G3X9S7 yet -( as G3X9S7 entry only created 11/2011) - it is not stated in any Ensembl External Refs for the longest 1912 aa transcript given - though this is the same protein from blasting Ensembl ENSMUSP00000102910/ENSMUST00000107289 Mouse IL1RAPL1-ECD-Fc was immobilised on Protein A-Sepharose beads. mishina@m.u-tokyo.ac.jp Only protein-protein interactions human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. competition binding competition binding immunostaining immunostaining Yoshida T., Yasumura M., Uemura T., Lee S.J., Ra M., Taguchi R., Iwakura Y., Mishina M. Synapse - Interactions of proteins with an established role in the presynapse. I have added an InterPro ref for ptprd_mouse -phosphatase subunit at Feature level - hope this is right place. Mouse IL1RAPL1-ECD-Fc was immobilised on Protein A-Sepharose beads. mishina@m.u-tokyo.ac.jp Accepted 2012-JUN-18 by ORCHARD J. Neurosci. (0270-6474) 2011 Only protein-protein interactions imex curation date:28/05/12 The entry G3X9S7 has now been incorporated into the reviwed entry Q64487 (PTPRD_MOUSE) - though I was not notified of this! Canonical sequence is now Q64487-4. I have altered sequence info for features for ALL Expts inc accepted ones. IL-1 receptor accessory protein-like 1 associated with mental retardation and autism mediates synapse formation by trans-synaptic interaction with protein tyrosine phosphatase δ. IL-1 receptor accessory protein-like 1 associated with mental retardation and autism mediates synapse formation by trans-synaptic interaction with protein tyrosine phosphatase δ. J. Neurosci. (0270-6474) 2011 imex curation Yoshida T., Yasumura M., Uemura T., Lee S.J., Ra M., Taguchi R., Iwakura Y., Mishina M. Synapse - Interactions of proteins with an established role in the presynapse. I have added an InterPro ref for ptprd_mouse -phosphatase subunit at Feature level - hope this is right place. DATE 8/3/12:- Wei-Muns reply to my isoform chasing email:- "Sorry for the delay - I'll do a quick update of the entry for the isoforms requested and merge only the isoforms as a priority." I'll send the update next week - however since the changes have to be integrated at SIB it may take the changes a little while before they are reflected in the database - but it should be in the next 2-3 weeks. " As a result, I do not anticipate any quick resolution to this. Fig 5 B/C is the problem - isoforms used are not in UniProt and I cannot create new proteins as I dont know the full sequence of the proteins. I have only used G3X9S7 in Figs 5B/C and Fig 3 for Mass Spec as in these instances it is clear the MeA/B peptides have been detected. Otherwise (unless I have missed something) no particular isoform has been detected - so Reviewed entry used, as at least the domain locations can be guessed at. Written 7/2/12:-(there may have been updates in Ensembl/UniProt since) From Fig 5A - Trembl entry G3X9S7 is currently the best entry for protein Ptprd/PTP-Delta -1912 aas and longest transcript, and includes MeA and MeB sequences given in fig 5A. ( The only Reviewed UniProt entry does NOT, and is taken from a shorter protein- entry added ~year 1993/5). Ensembl do not seem to have caught up with G3X9S7 yet -( as G3X9S7 entry only created 11/2011) - it is not stated in any Ensembl External Refs for the longest 1912 aa transcript given - though this is the same protein from blasting Ensembl ENSMUSP00000102910/ENSMUST00000107289 Mouse IL1RAPL1-ECD-Fc was immobilised on Protein A-Sepharose beads. mishina@m.u-tokyo.ac.jp Only protein-protein interactions in vitro In vitro fluorescence imaging fluorescence microscopy fluorescence imaging immunostaining immunostaining Yoshida T., Yasumura M., Uemura T., Lee S.J., Ra M., Taguchi R., Iwakura Y., Mishina M. Synapse - Interactions of proteins with an established role in the presynapse. I have added an InterPro ref for ptprd_mouse -phosphatase subunit at Feature level - hope this is right place. Mouse IL1RAPL1-ECD-Fc was immobilised on Protein A-Sepharose beads. mishina@m.u-tokyo.ac.jp Accepted 2012-JUN-18 by ORCHARD J. Neurosci. (0270-6474) 2011 Only protein-protein interactions imex curation date:28/05/12 The entry G3X9S7 has now been incorporated into the reviwed entry Q64487 (PTPRD_MOUSE) - though I was not notified of this! Canonical sequence is now Q64487-4. I have altered sequence info for features for ALL Expts inc accepted ones. lrc4b_mouse Leucine-rich repeat-containing protein 4B Lrrc4b Lrig4 Netrin-G3 ligand protein protein mouse Mus musculus Mouse MAQAHIRGSPCPLLPPGRMSWPHGALLLLWLFSPPLRAGGGGVAVTSAAGGGSPPATSCPAACSCSNQASRVICTRRELAEVPASIPVNTRYLNLQENSIQVIRTDTFKHLRHLEILQLSKNLVRKIEVGAFNGLPSLNTLELFDNRLTTVPTQAFEYLSKLRELWLRNNPIESIPSYAFNRVPSLRRLDLGELKRLEYISEAAFEGLVNLRYLNLGMCNLKDIPNLTALVRLEELELSGNRLDLIRPGSFQGLTSLRKLWLMHAQVATIERNAFDDLKSLEELNLSHNNLMSLPHDLFTPLHRLERVHLNHNPWHCNCDVLWLSWWLKETVPSNTTCCARCHAPAGLKGRYIGELDQSHFTCYAPVIVEPPTDLNVTEGMAAELKCRTGTSMTSVNWLTPNGTLMTHGSYRVRISVLHDGTLNFTNVTVQDTGQYTCMVTNSAGNTTASATLNVSAVDPVAAGGPGGGGPGGGGGAGGAGGYTYFTTVTVETLETQPGEEAQQPRGTEKEPPGPTTDGAWGGGRPDAAAPASASTTAPAPRSSRPTEKAFTVPITDVTENALKDLDDVMKTTKIIIGCFVAITFMAAVMLVAFYKLRKQHQLHKHHGPTRTVEIINVEDELPAASAVSVAAAAAVAGGAGVGGDSHLALPALERDHLNHHHYVAAAFKAHYGGNPGGGCGAKGPGLNSIHEPLLFKSGSKENVQETQI 0C39148F9A8E3586 irpl1_mouse Interleukin-1 receptor accessory protein-like 1 Il1rapl1 X-linked interleukin-1 receptor accessory protein-like 1 protein protein mouse Mus musculus Mouse MKAPIPHLILLYATFTQSLKVVTKRGSADGCTDWSVDIKKYQVLVGEPVRIKCALFYGYIRTNYSLAQSAGLSLMWYKSSGPGDFEEPIAFDGSRMSKEEDSIWFRPTLLQDSGLYACVIRNSTYCMKVSISLTVGENDTGLCYNSKMKYFEKAELSKSKEISCRDIEDFLLPTREPEILWYKECRTKAWRPSIVFKRDTLLIKEVKEDDIGNYTCELKYGGFVVRRTTELTVTAPLTDKPPKLLYPMESKLTVQETQLGGSANLTCRAFFGYSGDVSPLIYWMKGEKFIEDLDENRVWESDIRILKEHLGEQEVSISLIVDSVEEGDLGNYSCYVENGNGRRHASVLLHKRELMYTVELAGGLGAILLLLICSVTIYKCYKIEIMLFYRNHFGAEELDGDNKDYDAYLSYTKVDPDQWNQETGEEERFALEILPDMLEKHYGYKLFIPDRDLIPTGNIEDVARCVDQSKRLIIVMTPNYVVRRGWSIFELETRLRNMLVTGEIKVILIECSELRGIMNYQEVEALKHTIKLLTVIKWHGPKCNKLNSKFWKRLQYEMPFKRIEPITHEQALDVSEQGPFGELQTVSAISMAAATSTALATAHPDLRSTFHNTYHSQMRQKHYYRSYEYDVPPTGTLPLTSIGNQHTYCNIPMTLINGQRPQTKSNREPNPDEAHTNSAILPLLPRETSISSVIW 5FE34F204E5908B7 q64487-4 Receptor-type tyrosine-protein phosphatase delta delta Ptprd protein protein mouse Mus musculus Mouse MVPVARPLSLLLTFFLCACAETPPRFTRTPVDQTGVSGGVASFICQATGDPRPKIVWNKKGKKVSNQRFEVIEFDDGSGSVLRIQPLRTPRDEAIYECVASNNVGEISVSTRLTVLREDQIPRGFPTIDMGPQLKVVERTRTATMLCAASGNPDPEITWFKDFLPVDTSNNNGRIKQLRSESIGGTPIRGALQIEQSEESDQGKYECVATNSAGTRYSAPANLYVRELREVRRVPPRFSIPPTNHEIMPGGSVNITCVAVGSPMPYVKWMLGAEDLTPEDDMPIGRNVLELNDVRQSANYTCVAMSTLGVIEAIAQITVKALPKPPGTPVVTESTATSITLTWDSGNPEPVSYYIIQHKPKNSEEPYKEIDGIATTRYSVAGLSPYSDYEFRVVAVNNIGRGPASEPVLTQTSEQAPSSAPRDVQARMLSSTTILVQWKEPEEPNGQIQGYRVYYTMDPTQHVNNWMKHNVADSQITTIGNLVPQKTYSVKVLAFTSIGDGPLSSDIQVITQTGVPGQPLNFKAEPESETSILLSWTPPRSDTIASYELVYRDGDQGEEQRITIEPGTSYRLQGLKPNSLYYFRLSARSPQGLGASTAEISARTMQSKPSAPPQDISCTSPSSTSILVSWQPPPVEKQNGIITEYSLKYAAVDGEDFKPHEILGIPSDTTKYLLEQLEKWTEYRITVTAHTDVGPGPESLSVLIRTDEDVPSGPPRKVEVEAVNATAVKVSWRSPVPNKQHGQIRGYQVHYVKMENGEPKGQPMLKDVMLADAQWEFDDTTEHDMIISGLQPETSYSLTVTAYTTKGDGARSKPKLVSTTGAVPGKPRLVINHTQMNTALIQWHPPVDTFGPLQGYRLKFGRKDMEPLTTLEFSEKEDHFTATDIHKGASYVFRLSARNKVGFGEEMVKEISVPEEIPTGFPQNLHSEGTTSTSVQLSWQPPVLAERNGVITKYTLLYRDINVPLLPMEHLIVPADTSMTLTGLKSDTTYDVKVRAHTSKGPGPYSPSVQFRTLPVDQVFAKNFHVKAVMKTSVLLSWEIPENYNSAMPFKILYDDGKMVEEVDGRATQKLIVNLKPEKSYSFVLTNRGNSAGGLQHRVTAKTAPDVLRTKPAFIGKTNLDGMITVQLPDVPANENIKGYYIIIVPLKKSRGKFIKPWESPDEMELDELLKEISRKRRSIRYGREVELKPYIAAHFDVLPTEFTLGDDKHYGGFTNKQLQSGQEYVFFVLAVMDHAESKMYATSPYSDPVVSMDLDPQPITDEEEGLIWVVGPVLAVVFIICIVIAILLYKRKRAESESRKSSLPNSKEVPSHHPTDPVELRRLNFQTPGMASHPPIPILELADHIERLKANDNLKFSQEYESIDPGQQFTWEHSNLEVNKPKNRYANVIAYDHSRVLLSAIEGIPGSDYVNANYIDGYRKQNAYIATQGSLPETFGDFWRMIWEQRSATVVMMTKLEERSRVKCDQYWPSRGTETHGLVQVTLLDTVELATYCVRTFALYKNGSSEKREVRQFQFTAWPDHGVPEHPTPFLAFLRRVKTCNPPDAGPMVVHCSAGVGRTGCFIVIDAMLERIKHEKTVDIYGHVTLMRAQRNYMVQTEDQYIFIHDALLEAVTCGNTEVPARNLYAYIQKLTQIETGENVTGMELEFKRLASSKAHTSRFISANLPCNKFKNRLVNIMPYESTRVCLQPIRGVEGSDYINASFLDGYRQQKAYIATQGPLAETTEDFWRMLWEHNSTIVVMLTKLREMGREKCHQYWPAERSARYQYFVVDPMAEYNMPQYILREFKVTDARDGQSRTVRQFQFTDWPEQGVPKSGEGFIDFIGQVHKTKEQFGQDGPISVHCSAGVGRTGVFITLSIVLERMRYEGVVDIFQTVKMLRTQRPAMVQTEDQYQFCYRAALEYLGSFDHYAT DEF494AA5A2CE814 ptprd_mouse Receptor-type tyrosine-protein phosphatase delta Ptprd protein protein mouse Mus musculus Mouse MVPVARPLSLLLTFFLCACAETPPRFTRTPVDQTGVSGGVASFICQATGDPRPKIVWNKKGKKVSNQRFEVIEFDDGSGSVLRIQPLRTPRDEAIYECVASNNVGEISVSTRLTVLREDQIPRGFPTIDMGPQLKVVERTRTATMLCAASGNPDPEITWFKDFLPVDTSNNNGRIKQLRSESIGGTPIRGALQIEQSEESDQGKYECVATNSAGTRYSAPANLYVRELREVRRVPPRFSIPPTNHEIMPGGSVNITCVAVGSPMPYVKWMLGAEDLTPEDDMPIGRNVLELNDVRQSANYTCVAMSTLGVIEAIAQITVKALPKPPGTPVVTESTATSITLTWDSGNPEPVSYYIIQHKPKNSEEPYKEIDGIATTRYSVAGLSPYSDYEFRVVAVNNIGRGPASEPVLTQTSEQAPSSAPRDVQARMLSSTTILVQWKEPEEPNGQIQGYRVYYTMDPTQHVNNWMKHNVADSQITTIGNLVPQKTYSVKVLAFTSIGDGPLSSDIQVITQTGVPGQPLNFKAEPESETSILLSWTPPRSDTIASYELVYRDGDQGEEQRITIEPGTSYRLQGLKPNSLYYFRLSARSPQGLGASTAEISARTMQSKPSAPPQDISCTSPSSTSILVSWQPPPVEKQNGIITEYSLKYAAVDGEDFKPHEILGIPSDTTKYLLEQLEKWTEYRITVTAHTDVGPGPESLSVLIRTDEDVPSGPPRKVEVEAVNATAVKVSWRSPVPNKQHGQIRGYQVHYVKMENGEPKGQPMLKDVMLADAQWEFDDTTEHDMIISGLQPETSYSLTVTAYTTKGDGARSKPKLVSTTGAVPGKPRLVINHTQMNTALIQWHPPVDTFGPLQGYRLKFGRKDMEPLTTLEFSEKEDHFTATDIHKGASYVFRLSARNKVGFGEEMVKEISVPEEIPTGFPQNLHSEGTTSTSVQLSWQPPVLAERNGVITKYTLLYRDINVPLLPMEHLIVPADTSMTLTGLKSDTTYDVKVRAHTSKGPGPYSPSVQFRTLPVDQVFAKNFHVKAVMKTSVLLSWEIPENYNSAMPFKILYDDGKMVEEVDGRATQKLIVNLKPEKSYSFVLTNRGNSAGGLQHRVTAKTAPDVLRTKPAFIGKTNLDGMITVQLPDVPANENIKGYYIIIVPLKKSRGKFIKPWESPDEMELDELLKEISRKRRSIRYGREVELKPYIAAHFDVLPTEFTLGDDKHYGGFTNKQLQSGQEYVFFVLAVMDHAESKMYATSPYSDPVVSMDLDPQPITDEEEGLIWVVGPVLAVVFIICIVIAILLYKRKRAESESRKSSLPNSKEVPSHHPTDPVELRRLNFQTPGMASHPPIPILELADHIERLKANDNLKFSQEYESIDPGQQFTWEHSNLEVNKPKNRYANVIAYDHSRVLLSAIEGIPGSDYVNANYIDGYRKQNAYIATQGSLPETFGDFWRMIWEQRSATVVMMTKLEERSRVKCDQYWPSRGTETHGLVQVTLLDTVELATYCVRTFALYKNGSSEKREVRQFQFTAWPDHGVPEHPTPFLAFLRRVKTCNPPDAGPMVVHCSAGVGRTGCFIVIDAMLERIKHEKTVDIYGHVTLMRAQRNYMVQTEDQYIFIHDALLEAVTCGNTEVPARNLYAYIQKLTQIETGENVTGMELEFKRLASSKAHTSRFISANLPCNKFKNRLVNIMPYESTRVCLQPIRGVEGSDYINASFLDGYRQQKAYIATQGPLAETTEDFWRMLWEHNSTIVVMLTKLREMGREKCHQYWPAERSARYQYFVVDPMAEYNMPQYILREFKVTDARDGQSRTVRQFQFTDWPEQGVPKSGEGFIDFIGQVHKTKEQFGQDGPISVHCSAGVGRTGVFITLSIVLERMRYEGVVDIFQTVKMLRTQRPAMVQTEDQYQFCYRAALEYLGSFDHYAT DEF494AA5A2CE814 il1rapl1-ptprd-10 4 11 unspecified role unspecified role prey prey 9 unspecified role unspecified role bait bait region fc-igg tag fc-igg tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecd_region sufficient to bind sufficient binding region sufficient to bind range range range range physical association physical association Fig 3 LC-MS/MS identified the meA peptide in PTPdelta protein purified by the affinity chromatography with IL1RAPL1-ECD (Fig. 3B). il1rapl1-ptprd-1 3 11 unspecified role unspecified role prey prey phosphatase_subunit binding region binding-associated region binding region range range range range 9 unspecified role unspecified role bait bait region fc-igg tag fc-igg tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecd_region sufficient to bind sufficient binding region sufficient to bind range range range range 19 22 physical association physical association Fig 3B il1rapl1-ptprd-3 1 10 unspecified role unspecified role neutral component neutral component partial_me_a_region and me_b_region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain me_a_region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. 9 unspecified role unspecified role neutral component neutral component region fc-igg tag fc-igg tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecd_region sufficient to bind sufficient binding region sufficient to bind range range range range human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. 29 25 colocalization colocalization Fig 4A/4E, 5B/C. The meA and meB peptides of PTPdelta are critical for the interaction with IL1RAPL1. ptprd-il1rapl1 5 11 unspecified role unspecified role bait bait n_terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range 9 unspecified role unspecified role prey prey physical association physical association Fig 4B il1rapl1-ptprd-4 7 9 unspecified role unspecified role neutral component neutral component region egfp tag enhanced green fluorescent protein tag enhanced green fluorescent protein EGFP enhanced green fluorescent protein EGFP egfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. 11 unspecified role unspecified role neutral component neutral component region rfp tag red fluorescent protein tag red fluorescent protein RFP red fluorescent protein RFP rfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. colocalization colocalization Fig 4C The transfected cells aggregated into large clumps. il1rapl1-ptprd-8 2 9 unspecified role unspecified role bait bait region fc-igg tag fc-igg tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecd_region sufficient to bind sufficient binding region sufficient to bind range range range range 11 unspecified role unspecified role prey prey ecd_region binding region binding-associated region binding region range range range range region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 42 44 direct interaction direct interaction Fig 4D lrrc4b-ptprd 1 11 unspecified role unspecified role neutral component neutral component human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. 8 unspecified role unspecified role neutral component neutral component region fc-igg tag fc-igg tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecd_region sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. colocalization colocalization Fig 4E The intensity of staining signals for IL1RAPL1-ECD-Fc was much stronger than that for NGL-3-ECD-Fc. il1rapl1-ptprd-7 6 11 unspecified role unspecified role neutral component neutral component 9 unspecified role unspecified role neutral component neutral component ecd_region sufficient to bind sufficient binding region sufficient to bind range range range range region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Fig 4G NGL-3-ECD-Fc hardly affected the interaction between PTPdelta and IL1RAPL1-ECD tagged with His epitope. il1rapl1-ptprd-9 1 9 unspecified role unspecified role neutral component neutral component ecd_region sufficient to bind sufficient binding region sufficient to bind range range range range region fc-igg tag fc-igg tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. 10 unspecified role unspecified role neutral component neutral component me_a_region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain partial_me_a_region and me_b_region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. 62 59 colocalization colocalization Fig 4A/4E, 5B/C -2 The meA and meB peptides of PTPdelta are critical for the interaction with IL1RAPL1.