mpidb The Microbial Protein Interaction Database Role of leucine zipper motifs in association of the Escherichia coli cell division proteins FtsL and FtsB. Role of leucine zipper motifs in association of the Escherichia coli cell division proteins FtsL and FtsB. J. Bacteriol. (0021-9193) 2011 imex curation Robichon C., Karimova G., Beckwith J., Ladant D. 18-Dec-2012: Contacted by IntAct-Help. Only protein-protein interactions ecolx Escherichia coli anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Robichon C., Karimova G., Beckwith J., Ladant D. Accepted 2012-DEC-10 by ORCHARD 18-Dec-2012: Contacted by IntAct-Help. J. Bacteriol. (0021-9193) 2011 Only protein-protein interactions imex curation Role of leucine zipper motifs in association of the Escherichia coli cell division proteins FtsL and FtsB. Role of leucine zipper motifs in association of the Escherichia coli cell division proteins FtsL and FtsB. J. Bacteriol. (0021-9193) 2011 imex curation Robichon C., Karimova G., Beckwith J., Ladant D. 18-Dec-2012: Contacted by IntAct-Help. Only protein-protein interactions ecolx Escherichia coli adenylate cyclase adenylate cyclase complementation bacterial two-hybrid bacterial two-hybrid adenylate cyclase predetermined predetermined participant predetermined Robichon C., Karimova G., Beckwith J., Ladant D. Accepted 2012-DEC-10 by ORCHARD 18-Dec-2012: Contacted by IntAct-Help. J. Bacteriol. (0021-9193) 2011 Only protein-protein interactions imex curation ftsl_ecoli Cell division protein FtsL ftsL mraR b0083 JW0081 yabD protein protein ecoli Escherichia coli (strain K12) MISRVTEALSKVKGSMGSHERHALPGVIGDDLLRFGKLPLCLFICIILTAVTVVTTAHHTRLLTAQREQLVLERDALDIEWRNLILEENALGDHSRVERIATEKLQMQHVDPSQENIVVQK 6DD5DF7B2ECB7090 ftsb_ecoli Cell division protein FtsB ftsB b2748 JW2718 ygbQ protein protein ecoli Escherichia coli (strain K12) MGKLTLLLLAILVWLQYSLWFGKNGIHDYTRVNDDVAAQQATNAKLKARNDQLFAEIDDLNGGQEALEERARNELSMTRPGETFYRLVPDASKRAQSAGQNNR 9B50A7EF637809D7 ftsq_ecoli Cell division protein FtsQ ftsQ b0093 JW0091 protein protein ecoli Escherichia coli (strain K12) MSQAALNTRNSEEEVSSRRNNGTRLAGILFLLTVLTTVLVSGWVVLGWMEDAQRLPLSKLVLTGERHYTRNDDIRQSILALGEPGTFMTQDVNIIQTQIEQRLPWIKQVSVRKQWPDELKIHLVEYVPIARWNDQHMVDAEGNTFSVPPERTSKQVLPMLYGPEGSANEVLQGYREMGQMLAKDRFTLKEAAMTARRSWQLTLNNDIKLNLGRGDTMKRLARFVELYPVLQQQAQTDGKRISYVDLRYDSGAAVGWAPLPPEESTQQQNQAQAEQQ 839A3D34B948CEAB ftsq-ftsq-2 2 5 unspecified role unspecified role bait bait 5 unspecified role unspecified role prey prey physical association physical association figure 3C ftsq-ftsb-4 2 5 unspecified role unspecified role bait bait 4 unspecified role unspecified role prey prey physical association physical association figure 2B ftsq-ftsl-1 2 5 unspecified role unspecified role bait bait 3 unspecified role unspecified role prey prey physical association physical association Figure 2A ftsl-ftsb-6 2 4 unspecified role unspecified role prey prey P0A6S5:p.[Leu46Ala;Leu53Ala;Leu60Ala;Leu67Ala] P0A6S5-41-72 mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain L A certain certain sequence position certain certain certain sequence position certain L A certain certain sequence position certain certain certain sequence position certain L A certain certain sequence position certain certain certain sequence position certain L A Fri Jul 22 11:07:51 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'leu53ala,leu60ala,leu67ala,leu46ala' Wed May 02 14:27:42 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu46ala,leu53ala,leu60ala,leu67ala' Tue Jun 30 20:20:24 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Leu46Ala;Leu53Ala;Leu60Ala;Leu67Ala]' 3 unspecified role unspecified role bait bait P0AEN4:p.[Leu63Ala;Leu70Ala;Leu77Ala;Leu84Ala] mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain L A certain certain sequence position certain certain certain sequence position certain L A certain certain sequence position certain certain certain sequence position certain L A certain certain sequence position certain certain certain sequence position certain L A Fri Jul 22 11:10:48 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'leu70ala,leu63ala,leu77ala,leu84ala' Wed May 02 15:23:23 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu63ala,leu70ala,leu77ala,leu84ala' Tue Jun 30 18:02:28 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Leu63Ala;Leu70Ala;Leu77Ala;Leu84Ala]' physical association physical association Figure 2A ftsb-ftsl-11 2 4 unspecified role unspecified role bait bait P0A6S5:p.[Leu46Ala;Leu53Ala;Leu60Ala;Leu67Ala] P0A6S5-41-72 mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain L A certain certain sequence position certain certain certain sequence position certain L A certain certain sequence position certain certain certain sequence position certain L A certain certain sequence position certain certain certain sequence position certain L A Fri Jul 22 11:06:59 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'leu53ala,leu60ala,leu67ala,leu46ala' Wed May 02 19:53:02 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu46ala,leu53ala,leu60ala,leu67ala' Tue Jun 30 18:23:55 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Leu46Ala;Leu53Ala;Leu60Ala;Leu67Ala]' 3 unspecified role unspecified role prey prey P0AEN4:p.[Leu63Ala;Leu70Ala;Leu77Ala;Leu84Ala] mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain L A certain certain sequence position certain certain certain sequence position certain L A certain certain sequence position certain certain certain sequence position certain L A certain certain sequence position certain certain certain sequence position certain L A Fri Jul 22 11:04:35 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'leu70ala,leu63ala,leu77ala,leu84ala' Wed May 02 14:25:57 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu63ala,leu70ala,leu77ala,leu84ala' Tue Jun 30 19:37:11 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Leu63Ala;Leu70Ala;Leu77Ala;Leu84Ala]' physical association physical association Figure 2B ftsb-ftsl-12 1 4 unspecified role unspecified role bait bait P0A6S5:p.[Leu46Ala;Leu53Ala;Leu60Ala;Leu67Ala] P0A6S5-41-72 mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain L A certain certain sequence position certain certain certain sequence position certain L A certain certain sequence position certain certain certain sequence position certain L A certain certain sequence position certain certain certain sequence position certain L A Fri Jul 22 11:07:11 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'leu53ala,leu60ala,leu67ala,leu46ala' Wed May 02 16:34:28 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu46ala,leu53ala,leu60ala,leu67ala' Tue Jun 30 20:09:51 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Leu46Ala;Leu53Ala;Leu60Ala;Leu67Ala]' region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 3 unspecified role unspecified role prey prey P0AEN4:p.[Leu63Ala;Leu70Ala;Leu77Ala;Leu84Ala] mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain L A certain certain sequence position certain certain certain sequence position certain L A certain certain sequence position certain certain certain sequence position certain L A certain certain sequence position certain certain certain sequence position certain L A Fri Jul 22 11:00:05 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'leu70ala,leu63ala,leu77ala,leu84ala' Wed May 02 18:10:58 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu63ala,leu70ala,leu77ala,leu84ala' Tue Jun 30 19:07:16 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Leu63Ala;Leu70Ala;Leu77Ala;Leu84Ala]' region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Figure 2C