mpidb The Microbial Protein Interaction Database Characterization of components of the Staphylococcus aureus mRNA degradosome holoenzyme-like complex. Characterization of components of the Staphylococcus aureus mRNA degradosome holoenzyme-like complex. J. Bacteriol. (0021-9193) 2011 imex curation Roux C.M., DeMuth J.P., Dunman P.M. 18-Dec-2012: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Roux C.M., DeMuth J.P., Dunman P.M. Accepted 2012-DEC-10 by ORCHARD 18-Dec-2012: Contacted by IntAct-Help. J. Bacteriol. (0021-9193) 2011 Only protein-protein interactions imex curation Characterization of components of the Staphylococcus aureus mRNA degradosome holoenzyme-like complex. Characterization of components of the Staphylococcus aureus mRNA degradosome holoenzyme-like complex. J. Bacteriol. (0021-9193) 2011 imex curation Roux C.M., DeMuth J.P., Dunman P.M. 18-Dec-2012: Contacted by IntAct-Help. Only protein-protein interactions ecolx Escherichia coli adenylate cyclase adenylate cyclase complementation bacterial two-hybrid bacterial two-hybrid adenylate cyclase predetermined predetermined participant predetermined Roux C.M., DeMuth J.P., Dunman P.M. Accepted 2012-DEC-10 by ORCHARD 18-Dec-2012: Contacted by IntAct-Help. J. Bacteriol. (0021-9193) 2011 Only protein-protein interactions imex curation csha_staar DEAD-box ATP-dependent RNA helicase CshA cshA SAR2168 protein protein strain mrsa252 Staphylococcus aureus MQNFKELGISDNTVQSLESMGFKEPTPIQKDSIPYALQGIDILGQAQTGTGKTGAFGIPLIEKVVGKQGVQSLILAPTRELAMQVAEQLREFSRGQGVQVVTVFGGMPIERQIKALKKGPQIVVGTPGRVIDHLNRRTLKTDGIHTLILDEADEMMNMGFIDDMRFIMDKIPAVQRQTMLFSATMPKAIQALVQQFMKSPKIIKTMNNEMSDPQIEEFYTIVKELEKFDTFTNFLDVHQPELAIVFGRTKRRVDELTSALISKGYKAEGLHGDITQAKRLEVLKKFKNDQINILVATDVAARGLDISGVSHVYNFDIPQDTESYTHRIGRTGRAGKEGIAVTFVNPIEMDYIRQIEDANGRKMSALRPPHRKEVLQAREDDIKEKVENWMSKESESRLKRISTELLNEYNDVDLVAALLQELIEANDEVEVQLTFEKPLSRKGRNGKPSGSRNRNSKRGNPKFDSKSKRSKGYSSKKKSTKKFDRKEKSSGGSRPMKGRTFADHQK 8FB4525A6B4814AE eno_staar Enolase eno 2-phospho-D-glycerate hydro-lyase 2-phosphoglycerate dehydratase SAR0832 protein protein strain mrsa252 Staphylococcus aureus MPIITDVYAREVLDSRGNPTVEVEVLTESGAFGRALVPSGASTGEHEAVELRDGDKSRYLGKGVTKAVENVNEIIAPEIIEGEFSVLDQVSIDKMMIALDGTPNKGKLGANAILGVSIAVARAAADLLGQPLYKYLGGFNGKQLPVPMMNIVNGGSHSDAPIAFQEFMILPVGATTFKESLRWGTEIFHNLKSILSKRGLETAVGDEGGFAPKFEGTEDAVETIIQAIEAAGYKPGEEVFLGFDCASSEFYENGVYDYSKFEGEHGAKRTAAEQVDYLEQLVDKYPIITIEDGMDENDWDGWKQLTERIGDRVQLVGDDLFVTNTEILAKGIENGIGNSILIKVNQIGTLTETFDAIEMAQKAGYTAVVSHRSGETEDTTIADIAVATNAGQIKTGSLSRTDRIAKYNQLLRIEDELFETAKYDGIKSFYNLDK 756D40CED18865D7 pfka_staar ATP-dependent 6-phosphofructokinase pfkA Phosphohexokinase pfk SAR1777 protein protein strain mrsa252 Staphylococcus aureus MKKIAVLTSGGDSPGMNAAVRAVVRTAIYNEIEVYGVYHGYQGLLNDDIHKLELGSVGDTIQRGGTFLYSARCPEFKEQEVRKVAIENLRKRGIEGLVVIGGDGSYRGAQRISEECKEIQTIGIPGTIDNDINGTDFTIGFDTALNTIIGLVDKIRDTASSHARTFIIEAMGRDCGDLALWAGLSVGAETIVVPEVKTDIKEIADKIEQGIKRGKKHSIVLVAEGCMTAQDCQKELSQFINVDNRVSVLGHVQRGGSPTGADRVLASRLGGYAVDLLMQGETAKGVGIKNNKIVATSFDEIFDGKDHKFDYSLYELANKLSI E14141D6C0C0618B rnpa_bacsu Ribonuclease P protein component rnpA Protein C5 BSU41050 protein protein bacsu Bacillus subtilis MKKRNRLKKNEDFQKVFKHGTSVANRQFVLYTLDQPENDELRVGLSVSKKIGNAVMRNRIKRLIRQAFLEEKERLKEKDYIIIARKPASQLTYEETKKSLQHLFRKSSLYKKSSSK E15CA6B7851716BE rnj2_bacsu Ribonuclease J2 rnjB ymfA BSU16780 protein protein bacsu Bacillus subtilis MKKKNTENVRIIALGGVGEIGKNLYVIEIDSDIFVVDAGLMHPENEMLGIDVVIPDISYLIERADRVKAIFLTHGHDENIGGVFYLLNKLSVPVYGTKLTLALLREKLKQYGHNRKTDLREIHSKSVITFESTKVSFFRTIHSIPDSVGVSFKTSLGSIVCTGDFKFDQTPALNQTCDIGEIAKIGNSGVLALLSDSANAERPGYTPSEAAVSGEISDALYNSQNRVIIAVFASNINRIQQVIHAAAQNGRKIAVAGKNLQSVLQLARKLGYIEADDELFISVQDVKKYPKREVAIITAGSQGEPLAALTRMANKAHKQLNIEEGDTVVIASTPIPGQELIYSKTVDLLARAGAQVIFAQKRVHVSGHGSQEELKLMINLLKPKYLIPVNGEYRMQKAHSKIAEETGMKRSDIFLIEKGDVVEFRGQNVKIGDKVPYGNILIDGLGVGDIGNIVLRDRRLLSQDGILIVVITLDKQKKHLVSGPEIITRGFVYVRESEGLIVQATELVRSIVTEATETSNVEWSTLKQAMRDALNQFLYEKTKRKPMIIPIIMEV 8EA92EE42D51F282 8EA92EE42D51F282 pnp_staar Polyribonucleotide nucleotidyltransferase pnp pnpA SAR1250 Polynucleotide phosphorylase protein protein strain mrsa252 Staphylococcus aureus MSQEKKVFKTEWAGRSLTIETGQLAKQANGAVLVRYGDTVVLSTATASKEPRDGDFFPLTVNYEEKMYAAGKIPGGFKKREGRPGDDATLTARLIDRPIRPLFPKGYKHDVQIMNMVLSADPDCSPQMAAMIGSSMALSVSDIPFQGPIAGVNVGYIDGKYIINPTVEEKEVSRLDLEVAGHKDAVNMVEAGASEITEQEMLEAIFFGHEEIQRLVDFQQQIVDHIQPVKQEFIPTERDEALVERVKSLTEEKGLKETVLTFDKQQRDENLDNLKEEIVNEFIDEEDPENELLIKEVYAILNELVKEEVRRLIADEKIRPDGRKPDEIRPLDSEVGILPRTHGSGLFTRGQTQALSVLTLGALGDYQLIDGLGPEEEKRFMHHYNFPNFSVGETGPVRAPGRREIGHGALGERALKYIIPDTADFPYTIRIVSEVLESNGSSSQASICGSTLALMDAGVPIKAPVAGIAMGLVTREDSYTILTDIQGMEDALGDMDFKVAGTKEGITAIQMDIKIDGLTREIIEEALEQARRGRLEIMNHMLQTIDQPRTELSAYAPKVVTMTIKPDKIRDVIGPGGKKINEIIDETGVKLDIEQDGTIFIGAVDQAMINRAREIIEEITREAEVGQTYQATVKRIEKYGAFVGLFPGKDALLHISQISKNRIEKVEDVLKIGDTIEVKITEIDKQGRVNASHRALEE 06C4666428CCB4F0 rnj1_bacsu Ribonuclease J1 rnjA ykqC BSU14530 protein protein bacsu Bacillus subtilis MKFVKNDQTAVFALGGLGEIGKNTYAVQFQDEIVLIDAGIKFPEDELLGIDYVIPDYTYLVKNEDKIKGLFITHGHEDHIGGIPYLLRQVNIPVYGGKLAIGLLRNKLEEHGLLRQTKLNIIGEDDIVKFRKTAVSFFRTTHSIPDSYGIVVKTPPGNIVHTGDFKFDFTPVGEPANLTKMAEIGKEGVLCLLSDSTNSENPEFTMSERRVGESIHDIFRKVDGRIIFATFASNIHRLQQVIEAAVQNGRKVAVFGRSMESAIEIGQTLGYINCPKNTFIEHNEINRMPANKVTILCTGSQGEPMAALSRIANGTHRQISINPGDTVVFSSSPIPGNTISVSRTINQLYRAGAEVIHGPLNDIHTSGHGGQEEQKLMLRLIKPKFFMPIHGEYRMQKMHVKLATDCGIPEENCFIMDNGEVLALKGDEASVAGKIPSGSVYIDGSGIGDIGNIVLRDRRILSEEGLVIVVVSIDMDDFKISAGPDLISRGFVYMRESGDLINDAQELISNHLQKVMERKTTQWSEIKNEITDTLAPFLYEKTKRRPMILPIIMEV 48091B448B8431C9 48091B448B8431C9 rny_staar Ribonuclease Y rny cvfA SAR1262 Conserved virulence factor A protein protein strain mrsa252 Staphylococcus aureus MNLLSLLLILLGIILGVVGGYVVARNLLLQKQSQARQTAEDIVNQAHKEADNIKKEKLLEAKEENQILREQTEAELRERRSELQRQETRLLQKEENLERKSDLLDKKDEILEQKESKIEEKQQQVDAKESSVQTLIMKHEQELERISGLTQEEAINEQLQRVEEELSQDIAVLVKEKEKEAKEKVDKTAKELLATAVQRLAADHTSESTVSVVNLPNDEMKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVILSGFDPIRREIARTALVNLVSDGRIHPGRIEDMVEKARKEVDDIIREAGEQATFEVNAHNMHPDLVKIVGRLNYRTSYGQNVLKHSIEVAHLASMLAAELGEDETLAKRAGLLHDVGKAIDHEVEGSHVEIGVELAKKYGENETVINAIHSHHGDVEPTSIISILVAAADALSAARPGARKETLENYIRRLERLETLSESYDGVEKAFAIQAGREIRVIVSPEEIDDLKSYRLARDIKNQIEDELQYPGHIKVTVVRETRAVEYAK 076DE375A553FFA3 rnj2_staar Ribonuclease J 2 rnj2 SAR1251 protein protein strain mrsa252 Staphylococcus aureus MSLIKKKNKDIRIIPLGGVGEIAKNMYIVEVDDEMFMLDAGLMFPEDEMLGIDIVIPDISYVLENKEKLKGIFLTHGHEHAIGAVSYVLEQLDAPVYGSKLTIALIKENMKARNIDKKVRYYTVNNDSIMRFKNVNISFFNTTHSIPDSLGVCIHTSYGAIVYTGEFKFDQSLHGHYAPDIKRMAEIGEEGVFVLISDSTEAEKPGYNTPENVIEHHMYDAFAKVRGRLIVSCYASNFIRIQQVLNIASKLNRKVSFLGRSLESSFNIARKMGYFDIPKDLLIPITEVDNYPKNEVIIIATGMQGEPVEALSQMAQHKHKIMNIEEGDSVFLAITASANMEVIIANTLNELVRAGAHIIPNNKKIHASSHGCMEELKMMINIMKPEYFIPVQGEFKMQIAHAKLAAEAGVAPEKIFLVEKGDVINYNGKDMILNEKVNSGNILIDGIGIGDVGNIVLRDRHLLAEDGIFIAVVTLDPKNRRIAAGPEIQSRGFVYVRESEDLLREAEEKVREIVEAGLQEKRIEWSEIKQNMRDQISKLLFESTKRRPMIIPVISEI 2FE4B822D3922D2D rnj1_staar Ribonuclease J 1 rnj1 SAR1063 protein protein strain mrsa252 Staphylococcus aureus MKQLHPNEVGVYALGGLGEIGKNTYAVEYKDEIVIIDAGIKFPDDNLLGIDYVIPDYTYLVQNQDKIVGLFITHGHEDHIGGVPFLLKQLNIPIYGGPLALGLIRNKLEEHHLLRTAKLNEINEDSVIKSKHFTISFYLTTHSIPETYGVIVDTPEGKVVHTGDFKFDFTPVGKPANIAKMAQLGEEGVLCLLSDSTNSLVPDFTLSEREVGQNVDKIFRNCKGRIIFATFASNIYRVQQAVEAAIKNNRKIVTFGRSMENNIKIGMELGYIKAPPETFIEPNKINTVPKHELLILCTGSQGEPMAALSRIANGTHKQIKIIPEDTVVFSSSPIPGNTKSINRTINSLYKAGADVIHSKISNIHTSGHGSQGDQQLMLRLIKPKYFLPIHGEYRMLKAHGETGVECGVEEDNVFIFDIGDVLALTHDSARKAGRIPSGNVLVDGSGIGDIGNVVIRDRKLLSEEGLVIVVVSIDFNTNKLLSGPDIISRGFVYMRESGQLIYDAQRKIKTDVISKLNQNKDIQWHQIKSSIIETLQPYLFEKTARKPMILPVIMKVNEQKESNNK B1C619591C2E42B9 csha_bacsu DEAD-box ATP-dependent RNA helicase CshA cshA ydbR BSU04580 protein protein bacsu Bacillus subtilis MTITFQDFNLSSDLMKAINRMGFEEATPIQAQTIPLGLSNKDVIGQAQTGTGKTAAFGIPLVEKINPESPNIQAIVIAPTRELAIQVSEELYKIGQDKRAKVLPIYGGQDIGRQIRALKKNPNIIVGTPGRLLDHINRRTIRLNNVNTVVMDEADEMLNMGFIDDIESILSNVPSEHQTLLFSATMPAPIKRIAERFMTEPEHVKVKAKEMTVSNIQQFYLEVQERKKFDTLTRLLDIQSPELAIVFGRTKRRVDELAEALNLRGYAAEGIHGDLTQAKRMVALRKFKEGAIEVLVATDVAARGLDISGVTHVYNFDVPQDPESYVHRIGRTGRAGKTGMAMTFITPREKSMLRAIEQTTKRKMDRMKEPTLDEALEGQQQVTVERLRTTISENNLNFYMTAAAELLEDHDAVTVVAAAIKMATKEPDDTPVRLTDEAPMVSKRYKNQRSSKRRDGQGGGYRGGKGKSNNRSSYDKKRSNDRRSSGDRRQKKSY A44C5EAE314E46ED rnpa_staar Ribonuclease P protein component rnpA Protein C5 SAR2799 protein protein strain mrsa252 Staphylococcus aureus MEKAYRIKKNADFQRIYKKGHSVANRQFVVYTCNNKEIDHFRLGISVSKKLGNAVLRNKIKRAIRENFKVHKSHILAKDIIVIARQPAKDMTTLQIQNSLEHVLKIAKVFNKKIK 5C1D3F30AF2AEA9D rnpa-rnjb 2 6 unspecified role unspecified role bait bait 7 unspecified role unspecified role prey prey physical association physical association figure 3 rnpa-csha 2 13 unspecified role unspecified role prey prey 6 unspecified role unspecified role bait bait physical association physical association figure 3 rnpa-rnpa 2 6 unspecified role unspecified role bait bait 6 unspecified role unspecified role prey prey physical association physical association figure 3 rnja-rnjb 2 7 unspecified role unspecified role prey prey 9 unspecified role unspecified role bait bait physical association physical association figure 3 rnja-rnja 2 9 unspecified role unspecified role bait bait 9 unspecified role unspecified role prey prey physical association physical association figure 3 rny-rny 2 10 unspecified role unspecified role prey prey 10 unspecified role unspecified role bait bait physical association physical association figure 2 y2168-rny-1 2 10 unspecified role unspecified role prey prey 3 unspecified role unspecified role bait bait physical association physical association figure 2 eno-rny-2 2 10 unspecified role unspecified role prey prey 4 unspecified role unspecified role bait bait physical association physical association figure 2 rnpa-rnpa-1 2 14 unspecified role unspecified role bait bait 14 unspecified role unspecified role prey prey physical association physical association figure 2 y2168-rnpa 2 14 unspecified role unspecified role prey prey 3 unspecified role unspecified role bait bait physical association physical association figure 2 rnj2-rnj1 2 11 unspecified role unspecified role bait bait 12 unspecified role unspecified role prey prey physical association physical association figure 2 pnp-pnp-1 2 8 unspecified role unspecified role prey prey 8 unspecified role unspecified role bait bait physical association physical association figure 2 rnj1-rnj2 2 12 unspecified role unspecified role bait bait 11 unspecified role unspecified role prey prey physical association physical association figure 2 eno-rny 2 4 unspecified role unspecified role bait bait 10 unspecified role unspecified role prey prey physical association physical association figure 2 y2168-pfka 2 5 unspecified role unspecified role prey prey 3 unspecified role unspecified role bait bait physical association physical association figure 2 y2168-eno 2 4 unspecified role unspecified role prey prey 3 unspecified role unspecified role bait bait physical association physical association figure 2 pnp-pnp 2 8 unspecified role unspecified role prey prey 8 unspecified role unspecified role bait bait physical association physical association figure 2 pfka-pfka 2 5 unspecified role unspecified role bait bait 5 unspecified role unspecified role prey prey physical association physical association figure 2 eno-pnp 2 8 unspecified role unspecified role prey prey 4 unspecified role unspecified role bait bait physical association physical association figure 2 rnj2-rnj2-1 2 11 unspecified role unspecified role unspecified role unspecified role 11 unspecified role unspecified role unspecified role unspecified role physical association physical association figure 4 rnj1-rnj2-1 2 11 unspecified role unspecified role suppressor suppressor gene suppressor 12 unspecified role unspecified role unspecified role unspecified role physical association physical association figure 4 eno-eno-4 2 4 unspecified role unspecified role prey prey 4 unspecified role unspecified role bait bait physical association physical association figure 2 rnpa-y2168 2 3 unspecified role unspecified role prey prey 14 unspecified role unspecified role bait bait physical association physical association figure 2 rnj2-2 1 11 unspecified role unspecified role neutral component neutral component ecolx Escherichia coli direct interaction direct interaction Fig. 4 rnj1-rnj2-2 1 12 unspecified role unspecified role neutral component neutral component ecolx Escherichia coli 11 unspecified role unspecified role neutral component neutral component ecolx Escherichia coli direct interaction direct interaction Fig. 4