I2D I2D Semaphorin 5A and plexin-B3 regulate human glioma cell motility and morphology through Rac1 and the actin cytoskeleton. Semaphorin 5A and plexin-B3 regulate human glioma cell motility and morphology through Rac1 and the actin cytoskeleton. Oncogene (0950-9232) 2012 imex curation Li X., Law J.W., Lee A.Y. Cancer - Interactions investigated in the context of cancer Author confirms that the cytoplasmic domain of mouse plexin-B3 corresponds to aa1316-1902 of NP_062533 or A2AFF9 (Uniprot). phsleeyw@nus.edu.sg 04-Feb-2013: Contacted by IntAct-Help. Only protein-protein interactions Curated by: I2D - Interologous Interaction Database in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Li X., Law J.W., Lee A.Y. Cancer - Interactions investigated in the context of cancer Author confirms that the cytoplasmic domain of mouse plexin-B3 corresponds to aa1316-1902 of NP_062533 or A2AFF9 (Uniprot). phsleeyw@nus.edu.sg Accepted 2013-JAN-21 by ORCHARD 04-Feb-2013: Contacted by IntAct-Help. Oncogene (0950-9232) 2012 Only protein-protein interactions imex curation Curated by: I2D - Interologous Interaction Database Semaphorin 5A and plexin-B3 regulate human glioma cell motility and morphology through Rac1 and the actin cytoskeleton. Semaphorin 5A and plexin-B3 regulate human glioma cell motility and morphology through Rac1 and the actin cytoskeleton. Oncogene (0950-9232) 2012 imex curation Li X., Law J.W., Lee A.Y. Cancer - Interactions investigated in the context of cancer Author confirms that the cytoplasmic domain of mouse plexin-B3 corresponds to aa1316-1902 of NP_062533 or A2AFF9 (Uniprot). phsleeyw@nus.edu.sg 04-Feb-2013: Contacted by IntAct-Help. Only protein-protein interactions Curated by: I2D - Interologous Interaction Database in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Li X., Law J.W., Lee A.Y. Cancer - Interactions investigated in the context of cancer Author confirms that the cytoplasmic domain of mouse plexin-B3 corresponds to aa1316-1902 of NP_062533 or A2AFF9 (Uniprot). phsleeyw@nus.edu.sg Accepted 2013-JAN-21 by ORCHARD 04-Feb-2013: Contacted by IntAct-Help. Oncogene (0950-9232) 2012 Only protein-protein interactions imex curation Curated by: I2D - Interologous Interaction Database Semaphorin 5A and plexin-B3 regulate human glioma cell motility and morphology through Rac1 and the actin cytoskeleton. Semaphorin 5A and plexin-B3 regulate human glioma cell motility and morphology through Rac1 and the actin cytoskeleton. Oncogene (0950-9232) 2012 imex curation Li X., Law J.W., Lee A.Y. Cancer - Interactions investigated in the context of cancer Author confirms that the cytoplasmic domain of mouse plexin-B3 corresponds to aa1316-1902 of NP_062533 or A2AFF9 (Uniprot). phsleeyw@nus.edu.sg 04-Feb-2013: Contacted by IntAct-Help. Only protein-protein interactions Curated by: I2D - Interologous Interaction Database human-u87 Human U-87 MG glioblastoma cell line u87 anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Li X., Law J.W., Lee A.Y. Cancer - Interactions investigated in the context of cancer Author confirms that the cytoplasmic domain of mouse plexin-B3 corresponds to aa1316-1902 of NP_062533 or A2AFF9 (Uniprot). phsleeyw@nus.edu.sg Accepted 2013-JAN-21 by ORCHARD 04-Feb-2013: Contacted by IntAct-Help. Oncogene (0950-9232) 2012 Only protein-protein interactions imex curation Curated by: I2D - Interologous Interaction Database Semaphorin 5A and plexin-B3 regulate human glioma cell motility and morphology through Rac1 and the actin cytoskeleton. Semaphorin 5A and plexin-B3 regulate human glioma cell motility and morphology through Rac1 and the actin cytoskeleton. Oncogene (0950-9232) 2012 imex curation Li X., Law J.W., Lee A.Y. Cancer - Interactions investigated in the context of cancer Author confirms that the cytoplasmic domain of mouse plexin-B3 corresponds to aa1316-1902 of NP_062533 or A2AFF9 (Uniprot). phsleeyw@nus.edu.sg 04-Feb-2013: Contacted by IntAct-Help. Only protein-protein interactions Curated by: I2D - Interologous Interaction Database yeast Saccharomyces cerevisiae Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Li X., Law J.W., Lee A.Y. Cancer - Interactions investigated in the context of cancer Author confirms that the cytoplasmic domain of mouse plexin-B3 corresponds to aa1316-1902 of NP_062533 or A2AFF9 (Uniprot). phsleeyw@nus.edu.sg Accepted 2013-JAN-21 by ORCHARD 04-Feb-2013: Contacted by IntAct-Help. mouse brain cDNA library Oncogene (0950-9232) 2012 Only protein-protein interactions imex curation Curated by: I2D - Interologous Interaction Database plxb3_human Plexin-B3 PLXNB3 KIAA1206 PLXN6 protein protein human Homo sapiens Human MCHAAQETPLLHHFMAPVMARWPPFGLCLLLLLLSPPPLPLTGAHRFSAPNTTLNHLALAPGRGTLYVGAVNRLFQLSPELQLEAVAVTGPVIDSPDCVPFRDPAECPQAQLTDNANQLLLVSSRAQELVACGQVRQGVCETRRLGDVAEVLYQAEDPGDGQFVAANTPGVATVGLVVPLPGRDLLLVARGLAGKLSAGVPPLAIRQLAGSQPFSSEGLGRLVVGDFSDYNNSYVGAFADARSAYFVFRRRGARAQAEYRSYVARVCLGDTNLYSYVEVPLACQGQGLIQAAFLAPGTLLGVFAAGPRGTQAALCAFPMVELGASMEQARRLCYTAGGRGPSGAEEATVEYGVTSRCVTLPLDSPESYPCGDEHTPSPIAGRQPLEVQPLLKLGQPVSAVAALQADGHMIAFLGDTQGQLYKVFLHGSQGQVYHSQQVGPPGSAISPDLLLDSSGSHLYVLTAHQVDRIPVAACPQFPDCASCLQAQDPLCGWCVLQGRCTRKGQCGRAGQLNQWLWSYEEDSHCLHIQSLLPGHHPRQEQGQVTLSVPRLPILDADEYFHCAFGDYDSLAHVEGPHVACVTPPQDQVPLNPPGTDHVTVPLALMFEDVTVAATNFSFYDCSAVQALEAAAPCRACVGSIWRCHWCPQSSHCVYGEHCPEGERTIYSAQEVDIQVRGPGACPQVEGLAGPHLVPVGWESHLALRVRNLQHFRGLPASFHCWLELPGELRGLPATLEETAGDSGLIHCQAHQFYPSMSQRELPVPIYVTQGEAQRLDNTHALYVILYDCAMGHPDCSHCQAANRSLGCLWCADGQPACRYGPLCPPGAVELLCPAPSIDAVEPLTGPPEGGLALTILGSNLGRAFADVQYAVSVASRPCNPEPSLYRTSARIVCVTSPAPNGTTGPVRVAIKSQPPGISSQHFTYQDPVLLSLSPRWGPQAGGTQLTIRGQHLQTGGNTSAFVGGQPCPILEPVCPEAIVCRTRPQAAPGEAAVLVVFGHAQRTLLASPFRYTANPQLVAAEPSASFRGGGRLIRVRGTGLDVVQRPLLSVWLEADAEVQASRAQPQDPQPRRSCGAPAADPQACIQLGGGLLQCSTVCSVNSSSLLLCRSPAVPDRAHPQRVFFTLDNVQVDFASASGGQGFLYQPNPRLAPLSREGPARPYRLKPGHVLDVEGEGLNLGISKEEVRVHIGRGECLVKTLTRTHLYCEPPAHAPQPANGSGLPQFVVQMGNVQLALGPVQYEAEPPLSAFPVEAQAGVGMGAAVLIAAVLLLTLMYRHKSKQALRDYQKVLVQLESLETGVGDQCRKEFTDLMTEMTDLSSDLEGSGIPFLDYRTYAERAFFPGHGGCPLQPKPEGPGEDGHCATVRQGLTQLSNLLNSKLFLLTLIHTLEEQPSFSQRDRCHVASLLSLALHGKLEYLTDIMRTLLGDLAAHYVHRNPKLMLRRTETMVEKLLTNWLSICLYAFLREVAGEPLYMLFRAIQYQVDKGPVDAVTGKAKRTLNDSRLLREDVEFQPLTLMVLVGPGAGGAAGSSEMQRVPARVLDTDTITQVKEKVLDQVYKGTPFSQRPSVHALDLEWRSGLAGHLTLSDEDLTSVTQNHWKRLNTLQHYKVPDGATVGLVPQLHRGSTISQSLAQRCPLGENIPTLEDGEEGGVCLWHLVKATEEPEGAKVRCSSLREREPARAKAIPEIYLTRLLSMKGTLQKFVDDTFQAILSVNRPIPIAVKYLFDLLDELAEKHGIEDPGTLHIWKTNSLLLRFWVNALKNPQLIFDVRVSDNVDAILAVIAQTFIDSCTTSEHKVGRDSPVNKLLYAREIPRYKQMVERYYADIRQSSPASYQEMNSALAELSGNYTSAPHCLEALQELYNHIHRYYDQIISALEEDPVGQKLQLACRLQQVAALVENKVTDL 11753CD58C7AC226 plxb3_mouse Plexin-B3 Plxnb3 Kiaa1206 Plexin-6 Plxn6 protein protein mouse Mus musculus Mouse MLTDFLQAPVMAPWSPFSLHLLLLFLPLLPLTRVHRFSVPNTSFNHLVLAPDQGKLYVGAVNHLFQLSPELKMESVAVTGPVIDSPDCVPFRDLAECPQAQLTDNANQLLLVSSRTQELVACGQVKQGVCEKRRLGDVTQVLYQAEDPGDGQFVAANTLGVTTVGLVVPLPGRDLLLVARGLAGKLSAGVPPLTVRQLAGPQPFSSEGLGRLVVGDFSDYNNSYVGAFSDAHSAYFVFRRRGARAQTEYRSYVARVCLRDVNLYSYVEMPLTCHGQGLIQAAFLTPDTLLGAFSAGTSQAQAALCAFPLADLDRSMEQARRLCYTTGGQGPSGMEEATVEYGVTSRCVTLPPDSPESYPCGDEHTPSPIAGRQPLEAQPLLQLGQSISAVAALQTDGHTIAFLGDTQGQLHKVFLNSSHGQVYHSQQVGPPGSAISPDLLVDSNGDHLYVLTAQQVDRILVAACPQFPNCTTCLQARDPLCGWCILQGRCTRRGECGRAAQPNHWLWSYEDNHCPYIQSLLPAQHPRQEQGQIILSVPRLPTLAMDEYFHCAFGGYNSLAQVEEPHVVCTTPPQDQMPPNPPGSDHVTLPLALMFEDVVLTATTFSFYDCSAVQALEVAAPCRACVSSLWRCHWCPQSSHCIYGEHCPEGEKAVYSAQEVDILVRGPEACPQVEGLASPQLVPVGWESHVTLHIQNLHYFQGLPALYHCWLELPGKLQKLPASLEETSRDSGLIHCQAQQFYPSMSQWELPVPIYVTRGEIQRLDNAGDLHVTLYDCAMGHPDCSHCQAANGSLSCLWCGDGQPACRYGPLCPPGAVEQLCPIPSIDVIEPLTGPPEGGLAITILGSNLGQAFNDVRNAVTVAGQPCNPDPSLYRISARIVCVTSPAPNGTAGPVQVAIKSRPPGISTQNFTYQDPVLLSLNPQWGPQAGGTQLTIHGQYLQTGGNISVFVGDQPCPIQEPVCPEAIICHTMPQTEPGEAVVLIVFGHVERKLLTTPFRYTANPQLVEAEPSVSFRGGGRVIRVRGTGLDVVWQPLLSVWLEDEPKVKALGVQAQDANPRRSCGAPAADPQACIHLESGLLQCSTLCSVNSSSLLLCHSPAVPDGALPKRVFFALDNMQVDFASASGGQGFLYQPNPRLAPLSHEGITHPYHLKPGHVLDVEGEGLNLGISKEEVQVHIGDGECLVKTLTLTHLYCEPPPQAPQPTNGSGTLPQFVVQMGNLRLALGPVQYEAESMMSTFPVEAQLGLGMGAAVLIAAVLLLTLMYRHKSKKALRDYQKVLVQLENLETGVGDQCRKEFTDLMTEMTDLTSDLEASGIPFLDYRTYAERAFFPGHVGCPLQPGLEGLGEEGRSVTVRQGLTQLSNLLNSKLFLLTLIHTLEEQPSFSQRDRCHVASLLSLALHSKLEYLTDIMRTLLGDLAAHYVHKNPKLMLRRTETMVEKLLTNWLSICLYTFLKEVAGEPLYMLFRAIKYQVDKGPVDAVTGKAKRTLNDSHLLREDVEFQPLTLMALVGPEADRAAGNSGVHRVPARVLDTDTITQVKEKVLDQIYKGTPFSQRPSVHSLDLEWRSGLAGHLTLSDEDLTSVTQNHWKRLNTLQHYKVPDGATVVLIPQVHNGGTVSQSLGQTGCPSGENTPMLEDGEEGGVRLWHLVKATEEAEGAKVRRSSLRDRERERSRAKAIPEIYLTRLLSMKGTLQKFVDDTFQAILSMNRPVPIAVKYLFDFLDELAEKHGIEDPETLHIWKTNSLLLRFWVNVLKNPQLIFDVQVSDNEDAILAVIAQTFIDSCMVSEHKVGRDSPVNKLLYAREIPRYKQMVEKYYADIRQSSPASYQEMNSALAELSGNYSSAPHCLEALRELYNHIHRYYDQIISALEEDPVAQKMQLACRLQQVAALVEYKVTDL 25F8DE49B6C98071 fscn1_human Fascin FSCN1 FAN1 HSN SNL Singed-like protein 55 kDa actin-bundling protein p55 protein protein human Homo sapiens Human MTANGTAEAVQIQFGLINCGNKYLTAEAFGFKVNASASSLKKKQIWTLEQPPDEAGSAAVCLRSHLGRYLAADKDGNVTCEREVPGPDCRFLIVAHDDGRWSLQSEAHRRYFGGTEDRLSCFAQTVSPAEKWSVHIAMHPQVNIYSVTRKRYAHLSARPADEIAVDRDVPWGVDSLITLAFQDQRYSVQTADHRFLRHDGRLVARPEPATGYTLEFRSGKVAFRDCEGRYLAPSGPSGTLKAGKATKVGKDELFALEQSCAQVVLQAANERNVSTRQGMDLSANQDEETDQETFQLEIDRDTKKCAFRTHTGKYWTLTATGGVQSTASSKNASCYFDIEWRDRRITLRASNGKFVTSKKNGQLAASVETAGDSELFLMKLINRPIIVFRGEHGFIGCRKVTGTLDANRSSYDVFQLEFNDGAYNIKDSTGKYWTVGSDSAVTSSGDTPVDFFFEFCDYNKVAIKVGGRYLKGDHAGVLKASAETVDPASLWEY DIP protein Q16658 original sequence version: 89 C1453714BED6109A fscn1_mouse Fascin Fscn1 Fan1 Snl Singed-like protein protein protein mouse Mus musculus Mouse MTANGTAEAVQIQFGLISCGNKYLTAEAFGFKVNASASSLKKKQIWTLEQPPDEAGSAAVCLRSHLGRYLAADKDGNVTCEREVPDGDCRFLVVAHDDGRWSLQSEAHRRYFGGTEDRLSCFAQSVSPAEKWSVHIAMHPQVNIYSVTRKRYAHLSARPADEIAVDRDVPWGVDSLITLAFQDQRYSVQTSDHRFLRHDGRLVARPEPATGFTLEFRSGKVAFRDCEGRYLAPSGPSGTLKAGKATKVGKDELFALEQSCAQVVLQAANERNVSTRQGMDLSANQDEETDQETFQLEIDRDTRKCAFRTHTGKYWTLTATGGVQSTASTKNASCYFDIEWCDRRITLRASNGKFVTAKKNGQLAASVETAGDSELFLMKLINRPIIVFRGEHGFIGCRKVTGTLDANRSSYDVFQLEFNDGAYNIKDSTGKYWTVGSDSSVTSSSDTPVDFFLEFCDYNKVALKVGGRYLKGDHAGVLKACAETIDPASLWEY DIP protein Q61553 original sequence version: 116 1C6DCD5211B74969 plxnb3-fscn1 2 6 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 8 unspecified role unspecified role prey prey region mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) direct interaction direct interaction 3a plxnb3-fscn1-1 1 8 unspecified role unspecified role prey prey mouse-brain Mus musculus brain brain Brain [cerebrum] 6 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) physical association physical association 3b plxnb3-fscn1-2 3 5 unspecified role unspecified role bait bait 7 unspecified role unspecified role prey prey physical association physical association 3c A fusion cDNA between the extracellular domain of Sema5A and the Fc region of human IgG fscn1-plxnb3 3 7 unspecified role unspecified role bait bait 5 unspecified role unspecified role prey prey physical association physical association 3c A fusion cDNA between the extracellular domain of Sema5A and the Fc region of human IgG plxnb3-fscn1-3 4 6 unspecified role unspecified role bait bait cytoplasmic domain binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain 8 unspecified role unspecified role prey prey physical association physical association n/a