<?xml version='1.0' encoding='UTF-8'?>
<entrySet xmlns="http://psi.hupo.org/mi/mif300" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://psi.hupo.org/mi/mif300 https://raw.githubusercontent.com/HUPO-PSI/miXML/master/3.0/src/MIF300.xsd" level="3" version="0" minorVersion="0">
  <entry>
    <source releaseDate="2022-02-04">
      <names>
        <shortLabel>mpidb</shortLabel>
        <fullName>The Microbial Protein Interaction Database</fullName>
      </names>
      <xref>
        <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0903" refType="identity" refTypeAc="MI:0356"/>
        <secondaryRef db="intact" dbAc="MI:0469" id="EBI-6390654" refType="identity" refTypeAc="MI:0356"/>
      </xref>
    </source>
    <experimentList>
      <experimentDescription id="1">
        <names>
          <fullName>Effects of metal on the biochemical properties of Helicobacter pylori HypB, a maturation factor of [NiFe]-hydrogenase and urease.</fullName>
        </names>
        <bibref>
          <xref>
            <primaryRef db="pubmed" dbAc="MI:0446" id="21239585" refType="primary-reference" refTypeAc="MI:0358"/>
            <secondaryRef db="intact" dbAc="MI:0469" id="EBI-6413773" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="imex" dbAc="MI:0670" id="IM-18387" refType="imex-primary" refTypeAc="MI:0662"/>
          </xref>
          <attributeList>
            <attribute name="publication title" nameAc="MI:1091">Effects of metal on the biochemical properties of Helicobacter pylori HypB, a maturation factor of [NiFe]-hydrogenase and urease.</attribute>
            <attribute name="journal" nameAc="MI:0885">J. Bacteriol. (0021-9193)</attribute>
            <attribute name="publication year" nameAc="MI:0886">2011</attribute>
            <attribute name="curation depth" nameAc="MI:0955">imex curation</attribute>
            <attribute name="imex curation" nameAc="MI:0959"/>
            <attribute name="author-list" nameAc="MI:0636">Sydor A.M., Liu J., Zamble D.B.</attribute>
            <attribute name="author-announcement">18-Dec-2012: Contacted by IntAct-Help.</attribute>
            <attribute name="full coverage" nameAc="MI:0957">Only protein-protein interactions</attribute>
            <attribute name="imex curation" nameAc="MI:0959"/>
          </attributeList>
        </bibref>
        <xref>
          <primaryRef db="imex" dbAc="MI:0670" id="IM-18387" refType="imex-primary" refTypeAc="MI:0662"/>
          <secondaryRef db="pubmed" dbAc="MI:0446" id="21239585" refType="primary-reference" refTypeAc="MI:0358"/>
        </xref>
        <hostOrganismList>
          <hostOrganism ncbiTaxId="562">
            <names>
              <shortLabel>ecolx</shortLabel>
              <fullName>Escherichia coli</fullName>
            </names>
          </hostOrganism>
        </hostOrganismList>
        <interactionDetectionMethod>
          <names>
            <shortLabel>molecular sieving</shortLabel>
            <fullName>molecular sieving</fullName>
            <alias type="go synonym" typeAc="MI:0303">Gel Filtration</alias>
            <alias type="go synonym" typeAc="MI:0303">Size Exclusion Chromatography</alias>
            <alias type="synonym" typeAc="MI:1041">Size Exclusion Chromatography</alias>
            <alias type="synonym" typeAc="MI:1041">Gel Filtration</alias>
            <alias type="synonym" typeAc="MI:1041">Sizing column</alias>
          </names>
          <xref>
            <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0071" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="intact" dbAc="MI:0469" id="EBI-1175" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="pubmed" dbAc="MI:0446" id="7708014" refType="primary-reference" refTypeAc="MI:0358"/>
          </xref>
        </interactionDetectionMethod>
        <participantIdentificationMethod>
          <names>
            <shortLabel>predetermined</shortLabel>
            <fullName>predetermined participant</fullName>
            <alias type="synonym" typeAc="MI:1041">predetermined</alias>
          </names>
          <xref>
            <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0396" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="intact" dbAc="MI:0469" id="EBI-1465" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
          </xref>
        </participantIdentificationMethod>
        <attributeList>
          <attribute name="author-list" nameAc="MI:0636">Sydor A.M., Liu J., Zamble D.B.</attribute>
          <attribute name="accepted">Accepted 2012-DEC-11 by ORCHARD</attribute>
          <attribute name="author-announcement">18-Dec-2012: Contacted by IntAct-Help.</attribute>
          <attribute name="journal" nameAc="MI:0885">J. Bacteriol. (0021-9193)</attribute>
          <attribute name="publication year" nameAc="MI:0886">2011</attribute>
          <attribute name="full coverage" nameAc="MI:0957">Only protein-protein interactions</attribute>
          <attribute name="imex curation" nameAc="MI:0959"/>
          <attribute name="curation depth" nameAc="MI:0955">imex curation</attribute>
        </attributeList>
      </experimentDescription>
    </experimentList>
    <interactorList>
      <interactor id="2">
        <names>
          <shortLabel>hypb_helpy</shortLabel>
          <fullName>Hydrogenase/urease maturation factor HypB</fullName>
          <alias type="gene name" typeAc="MI:0301">hypB</alias>
          <alias type="locus name" typeAc="MI:0305">HP_0900</alias>
          <alias type="gene name synonym" typeAc="MI:0302">Hydrogenase/urease nickel incorporation protein HypB</alias>
        </names>
        <xref>
          <primaryRef db="uniprotkb" dbAc="MI:0486" id="O25560" version="SP_69" refType="identity" refTypeAc="MI:0356"/>
          <secondaryRef db="ensemblbacteria" id="AAD07946" version="SP_118" refType="identity" refTypeAc="MI:0356"/>
          <secondaryRef db="intact" dbAc="MI:0469" id="EBI-6449853" refType="identity" refTypeAc="MI:0356"/>
          <secondaryRef db="ensemblbacteria" id="AAD07946" version="SP_118" refType="transcript"/>
          <secondaryRef db="ensemblbacteria" id="HP_0900" version="SP_118" refType="gene"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0003924" version="SP_118"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0005525" version="SP_118"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0008270" version="SP_118"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0016151" version="SP_118"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0051604" version="SP_118"/>
          <secondaryRef db="interpro" dbAc="MI:0449" id="IPR003495" version="SP_118"/>
          <secondaryRef db="interpro" dbAc="MI:0449" id="IPR004392" version="SP_118"/>
          <secondaryRef db="interpro" dbAc="MI:0449" id="IPR027417" version="SP_118"/>
          <secondaryRef db="rcsb pdb" dbAc="MI:0460" id="4LPS" version="SP_118"/>
          <secondaryRef db="refseq" dbAc="MI:0481" id="NP_207693.1"/>
          <secondaryRef db="mint" dbAc="MI:0471" id="O25560"/>
          <secondaryRef db="refseq" dbAc="MI:0481" id="WP_000003622.1"/>
          <secondaryRef db="dip" dbAc="MI:0465" id="DIP-3235N"/>
        </xref>
        <interactorType>
          <names>
            <shortLabel>protein</shortLabel>
            <fullName>protein</fullName>
          </names>
          <xref>
            <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0326" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="intact" dbAc="MI:0469" id="EBI-619654" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
            <secondaryRef db="so" dbAc="MI:0601" id="SO:0000358" refType="see-also" refTypeAc="MI:0361"/>
          </xref>
        </interactorType>
        <organism ncbiTaxId="85962">
          <names>
            <shortLabel>helpy</shortLabel>
            <fullName>Helicobacter pylori</fullName>
            <alias type="synonym" typeAc="MI:1041">Campylobacter pylori</alias>
          </names>
        </organism>
        <sequence>MSEQRQESLQNNPNLSKKDVKIVEKILSKNDIKAAEMKERYLKEGLYVLNFMSSPGSGKTTMLENLADFKDFKFCVVEGDLQTNRDADRLRKKGVSAHQITTGEACHLEASMIEGAFDLLKDEGALEKSDFLIIENVGNLVCPSSYNLGAAMNIVLLSVPEGDDKVLKYPTMFMCADAVIISKADMVEVFNFRVSQVKEDMQKLKPEAPIFLMSSKDPKSLEDFKNFLLEKKRENYQSTHSF</sequence>
        <attributeList>
          <attribute name="crc64">7C505390FB5C0142</attribute>
        </attributeList>
      </interactor>
    </interactorList>
    <interactionList>
      <interaction id="3" imexId="IM-18387-1">
        <names>
          <shortLabel>hypb-3</shortLabel>
        </names>
        <xref>
          <primaryRef db="mpidb" dbAc="MI:0903" id="MPIDB-INT-1510" refType="identity" refTypeAc="MI:0356"/>
          <secondaryRef db="intact" dbAc="MI:0469" id="EBI-6413787" refType="identity" refTypeAc="MI:0356"/>
          <secondaryRef db="imex" dbAc="MI:0670" id="IM-18387-1" refType="imex-primary" refTypeAc="MI:0662"/>
        </xref>
        <experimentList>
          <experimentRef>1</experimentRef>
        </experimentList>
        <participantList>
          <participant id="4">
            <interactorRef>2</interactorRef>
            <biologicalRole>
              <names>
                <shortLabel>unspecified role</shortLabel>
                <fullName>unspecified role</fullName>
              </names>
              <xref>
                <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0499" refType="identity" refTypeAc="MI:0356"/>
                <secondaryRef db="intact" dbAc="MI:0469" id="EBI-77781" refType="identity" refTypeAc="MI:0356"/>
                <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
              </xref>
            </biologicalRole>
            <experimentalRoleList>
              <experimentalRole>
                <names>
                  <shortLabel>neutral component</shortLabel>
                  <fullName>neutral component</fullName>
                </names>
                <xref>
                  <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0497" refType="identity" refTypeAc="MI:0356"/>
                  <secondaryRef db="intact" dbAc="MI:0469" id="EBI-55" refType="identity" refTypeAc="MI:0356"/>
                  <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                </xref>
              </experimentalRole>
            </experimentalRoleList>
            <featureList>
              <feature id="5">
                <names>
                  <shortLabel>O25560:p.Cys106_His107delinsAlaAla</shortLabel>
                </names>
                <xref>
                  <primaryRef db="intact" dbAc="MI:0469" id="EBI-6449867" refType="identity" refTypeAc="MI:0356"/>
                </xref>
                <featureType>
                  <names>
                    <shortLabel>mutation decreasing strength</shortLabel>
                    <fullName>mutation decreasing interaction strength</fullName>
                    <alias type="synonym" typeAc="MI:1041">mutation decreasing strength</alias>
                  </names>
                  <xref>
                    <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:1133" refType="identity" refTypeAc="MI:0356"/>
                    <secondaryRef db="intact" dbAc="MI:0469" id="EBI-5528801" refType="identity" refTypeAc="MI:0356"/>
                    <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                  </xref>
                </featureType>
                <featureRangeList>
                  <featureRange>
                    <startStatus>
                      <names>
                        <shortLabel>certain</shortLabel>
                        <fullName>certain sequence position</fullName>
                        <alias type="synonym" typeAc="MI:1041">certain</alias>
                      </names>
                      <xref>
                        <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0335" refType="identity" refTypeAc="MI:0356"/>
                        <secondaryRef db="intact" dbAc="MI:0469" id="EBI-540564" refType="identity" refTypeAc="MI:0356"/>
                        <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                      </xref>
                    </startStatus>
                    <begin position="106"/>
                    <endStatus>
                      <names>
                        <shortLabel>certain</shortLabel>
                        <fullName>certain sequence position</fullName>
                        <alias type="synonym" typeAc="MI:1041">certain</alias>
                      </names>
                      <xref>
                        <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0335" refType="identity" refTypeAc="MI:0356"/>
                        <secondaryRef db="intact" dbAc="MI:0469" id="EBI-540564" refType="identity" refTypeAc="MI:0356"/>
                        <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                      </xref>
                    </endStatus>
                    <end position="107"/>
                    <resultingSequence>
                      <originalSequence>CH</originalSequence>
                      <newSequence>AA</newSequence>
                    </resultingSequence>
                  </featureRange>
                </featureRangeList>
                <attributeList>
                  <attribute name="remark-internal">Thu Jul 07 12:07:04 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was cys106_his107ala</attribute>
                  <attribute name="remark-internal">Fri Jul 22 10:59:17 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'cys106ala,his107ala'</attribute>
                  <attribute name="remark-internal">Wed May 02 16:48:05 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'cys_his106-107ala_ala'</attribute>
                  <attribute name="remark-internal">Tue Jun 30 17:41:28 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Cys106_His107delinsAlaAla'</attribute>
                </attributeList>
              </feature>
            </featureList>
            <stoichiometry value="2"/>
          </participant>
        </participantList>
        <interactionType>
          <names>
            <shortLabel>physical association</shortLabel>
            <fullName>physical association</fullName>
          </names>
          <xref>
            <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0915" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="intact" dbAc="MI:0469" id="EBI-1813147" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
          </xref>
        </interactionType>
        <attributeList>
          <attribute name="figure legend" nameAc="MI:0599">figure 4</attribute>
          <attribute name="comment" nameAc="MI:0612">Metal-dependent dimerization observed in the presence of Ni(II) but not Zn(II).</attribute>
        </attributeList>
      </interaction>
    </interactionList>
  </entry>
</entrySet>