UniProt UniProt http://www.uniprot.org Bi-modal regulation of a formin by srGAP2. Bi-modal regulation of a formin by srGAP2. J. Biol. Chem. (0021-9258) 2011 mimix curation Mason F.M., Heimsath E.G., Higgs H.N., Soderling S.H. Authors could not provide information concerning the origin of the Rac constructs s.soderling@cellbio.duke.edu in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Mason F.M., Heimsath E.G., Higgs H.N., Soderling S.H. Authors could not provide information concerning the origin of the Rac constructs s.soderling@cellbio.duke.edu Accepted 2012-JUL-05 by JYOTI J. Biol. Chem. (0021-9258) 2011 mimix curation Bi-modal regulation of a formin by srGAP2. Bi-modal regulation of a formin by srGAP2. J. Biol. Chem. (0021-9258) 2011 mimix curation Mason F.M., Heimsath E.G., Higgs H.N., Soderling S.H. Authors could not provide information concerning the origin of the Rac constructs s.soderling@cellbio.duke.edu yeast Saccharomyces cerevisiae Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Mason F.M., Heimsath E.G., Higgs H.N., Soderling S.H. Authors could not provide information concerning the origin of the Rac constructs s.soderling@cellbio.duke.edu Accepted 2012-JUL-05 by JYOTI J. Biol. Chem. (0021-9258) 2011 mimix curation Bi-modal regulation of a formin by srGAP2. Bi-modal regulation of a formin by srGAP2. J. Biol. Chem. (0021-9258) 2011 mimix curation Mason F.M., Heimsath E.G., Higgs H.N., Soderling S.H. Authors could not provide information concerning the origin of the Rac constructs s.soderling@cellbio.duke.edu in vitro In vitro peptide array peptide array autoradiography autoradiography Mason F.M., Heimsath E.G., Higgs H.N., Soderling S.H. Authors could not provide information concerning the origin of the Rac constructs s.soderling@cellbio.duke.edu Accepted 2012-JUL-05 by JYOTI J. Biol. Chem. (0021-9258) 2011 mimix curation Bi-modal regulation of a formin by srGAP2. Bi-modal regulation of a formin by srGAP2. J. Biol. Chem. (0021-9258) 2011 mimix curation Mason F.M., Heimsath E.G., Higgs H.N., Soderling S.H. Authors could not provide information concerning the origin of the Rac constructs s.soderling@cellbio.duke.edu human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Mason F.M., Heimsath E.G., Higgs H.N., Soderling S.H. Authors could not provide information concerning the origin of the Rac constructs s.soderling@cellbio.duke.edu Accepted 2012-JUL-05 by JYOTI J. Biol. Chem. (0021-9258) 2011 mimix curation Bi-modal regulation of a formin by srGAP2. Bi-modal regulation of a formin by srGAP2. J. Biol. Chem. (0021-9258) 2011 mimix curation Mason F.M., Heimsath E.G., Higgs H.N., Soderling S.H. Authors could not provide information concerning the origin of the Rac constructs s.soderling@cellbio.duke.edu human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Mason F.M., Heimsath E.G., Higgs H.N., Soderling S.H. Authors could not provide information concerning the origin of the Rac constructs s.soderling@cellbio.duke.edu Accepted 2012-JUL-05 by JYOTI J. Biol. Chem. (0021-9258) 2011 mimix curation Bi-modal regulation of a formin by srGAP2. Bi-modal regulation of a formin by srGAP2. J. Biol. Chem. (0021-9258) 2011 mimix curation Mason F.M., Heimsath E.G., Higgs H.N., Soderling S.H. Authors could not provide information concerning the origin of the Rac constructs s.soderling@cellbio.duke.edu yeast Saccharomyces cerevisiae Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Mason F.M., Heimsath E.G., Higgs H.N., Soderling S.H. Authors could not provide information concerning the origin of the Rac constructs s.soderling@cellbio.duke.edu Accepted 2012-JUL-05 by JYOTI J. Biol. Chem. (0021-9258) 2011 mimix curation robo2_mouse Roundabout homolog 2 Robo2 Kiaa1568 protein protein mouse Mus musculus Mouse MNPLMFTLLLLFGFLCIQIDGSRLRQEDFPPRIVEHPSDVIVSKGEPTTLNCKAEGRPTPTIEWYKDGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRRSKPDEGSYVCVARNYLGEAVSRNASLEVALLRDDFRQNPTDVVVAAGEPAILECQPPRGHPEPTIYWKKDKVRIDDKEERISIRGGKLMISNTRKSDAGMYTCVGTNMVGERDSDPAELTVFERPTFLRRPINQVVLEEEAVEFRCQVQGDPQPTVRWKKDDADLPRGRYDIKDDYTLRIKKAMSTDEGTYVCIAENRVGKVEASATLTVRVRPVAPPQFVVRPRDQIVAQGRTVTFPCETKGNPQPAVFWQKEGSQNLLFPNQPQQPNSRCSVSPTGDLTITNIQRSDAGYYICQALTVAGSILAKAQLEVTDVLTDRPPPIILQGPINQTLAVDGTALLKCKATGEPLPVISWLKEGFTFLGRDPRATIQDQGTLQIKNLRISDTGTYTCVATSSSGETSWSAVLDVTESGATISKNYDMNDLPGPPSKPQVTDVSKNSVTLSWQPGTPGVLPASAYIIEAFSQSVSNSWQTVANHVKTTLYTVRGLRPNTIYLFMVRAINPQGLSDPSPMSDPVRTQDISPPAQGVDHRQVQKELGDVVVRLHNPVVLTPTTVQVTWTVDRQPQFIQGYRVMYRQTSGLQASTVWQNLDAKVPTERSAVLVNLKKGVTYEIKVRPYFNEFQGMDSESKTVRTTEEAPSAPPQSVTVLTVGSHNSTSISVSWDPPPADHQNGIIQEYKIWCLGNETRFHINKTVDAAIRSVVIGGLFPGIQYRVEVAASTSAGVGVKSEPQPIIIGGRNEVVITENNNSITEQITDVVKQPAFIAGIGGACWVILMGFSIWLYWRRKKRKGLSNYAVTFQRGDGGLMSNGSRPGLLNAGDPNYPWLADSWPATSLPVNNSNSGPNEIGNFGRGDVLPPVPGQGDKTATMLSDGAIYSSIDFTTKTTYNSSSQITQATPYATTQILHSNSIHELAVDLPDPQWKSSVQQKTDLMGFGYSLPDQNKGNNGGKGGKKKKTKNSSKAQKNNGSTWANVPLPPPPVQPLPGTELGHYAAEQENGYDSDSWCPPLPVQTYLHQGMEDELEEDEDRVPTPPVRGVASSPAISFGQQSTATLTPSPREEMQPMLQAHLDELTRAYQFDIAKQTWHIQSNTPPPQPPAPPLGYVSGALISDLETDVPDEDADDEEEPLEIPRPLRALDQTPGSSMDNLDSSVTGKAFSSSQRQRPTSPFSTDSNTSAAQNQSQRPRPTKKHKGGRMDPQPVLPHRREGMPDDLPPPPDPPPGQGLRQQIGLSQHSGNVENSTERKGSSLERQQAANLEDTKSSLDCPAKTVLEWQRQTQDWINSTERQEETRKAPHKQGVGSEESLVPYSKPSFPSPGGHSSSGTSSSKGSTGPRKADVLRGSHQRNANDLLDIGYVGSNSQGQFTE 3C61DE40DA39109E fmnl1_mouse Formin-like protein 1 Formin-related protein Fmnl1 Frl Frl1 protein protein mouse Mus musculus Mouse MGNAAGSAEQPAGPTASPPKQPAVPKQPMPAAGELEERFTRVLNCMNLPPDKVQLLSQYDNEKKWELICDQERFQVKNPPAAYIQKLKSYLDTGGVSRKVASDWMSNLGFKRRVQESTQVLRELETSLRTNHIGWVQEFLNEENRGLDVLLEYLAFAQCSVAYDMESTDSVASGAEKSKPLDQSVEDLSKAPPSSVPKSRLTIKLTPAHSRKALRNSRIVSQKDDVHVCIMCLRAIMNYQSGFSLVMNHPACVNEIALSLNNKSPRTKALVLELLAAVCLVRGGHDIILAAFDNFKEVCGEQHRFEKLMEYFRHEDSNIDFMVACMQFINIVVHSVENMNFRVFLQYEFTHLGLDLYLERLRLTESDKLQVQIQAYLDNVFDVGTLLEETETKNAVLEHMEELQEQVATLTERLRDTENDSMAKIAELEKQLSQARKELETLRERFSESTPMGTSRRIPEPEKVPVPTVVRPSALELKVEELEEKGLIRILRGPGDVVSIEILPGAAATPSGDDAQAPRVSTDSPSTAESIPEAASPPPPPPPPPPPLPNLQSQQEAPPSAPPLAPPLPGCAEPPPAPPLPGDLPPPPPPPPLGTDGPVPPPPPPPPGGPPDILGGQGPDIGPGVKAKKPIQTKFRMPLLNWVALKPSQITGTVFTELNDEKVLQELDMNDFEEHFKTKSQGPCLDISALKGKASQKAPTKTILIEANRAKNLAITLRKGNLGADRICQAIETYDLQTLSLDFLELLTRFLPTDYERSLIARFEKEQRPMEELSEEDRFMLRFSRIQRLPERMNTLTFLGNFPDTAQLLMPQLNAIIAASMSIKSSDKLRQILEIVLAFGNYMNSSKRGAAYGFRLQSLDALLEMKSTDRKQTLLHYLVKVIAEKYPQLTGFHSDLHFLDKAGSVSLDSVLGDVRSLQRGLELTQREFVRQDDCLVLKEFLRANSPTMDKLLADSKTAQEAYESVVEYFGENPKTTSPSMFFSLFSRFTKAYKKAEQEVEQWKKEAAADTSGREEPPTPKSPPKARRQQMDLISELKRKQQKEPLIYESDRDGAIEDIITDLRNQPYIRADTGRRSARRRPPGPPLPVTTDLAL 23A4BF24FB8F7A68 fmnl1_human Formin-like protein 1 Leukocyte formin CLL-associated antigen KW-13 FMNL1 C17orf1 C17orf1B FMNL FRL1 protein protein human Homo sapiens Human MGNAAGSAEQPAGPAAPPPKQPAPPKQPMPAAGELEERFNRALNCMNLPPDKVQLLSQYDNEKKWELICDQERFQVKNPPAAYIQKLKSYVDTGGVSRKVAADWMSNLGFKRRVQESTQVLRELETSLRTNHIGWVQEFLNEENRGLDVLLEYLAFAQCSVTYDMESTDNGASNSEKNKPLEQSVEDLSKGPPSSVPKSRHLTIKLTPAHSRKALRNSRIVSQKDDVHVCIMCLRAIMNYQSGFSLVMNHPACVNEIALSLNNKNPRTKALVLELLAAVCLVRGGHDIILAAFDNFKEVCGEQHRFEKLMEYFRNEDSNIDFMVACMQFINIVVHSVENMNFRVFLQYEFTHLGLDLYLERLRLTESDKLQVQIQAYLDNIFDVGALLEDTETKNAVLEHMEELQEQVALLTERLRDAENESMAKIAELEKQLSQARKELETLRERFSESTAMGPSRRPPEPEKAPPAAPTRPSALELKVEELEEKGLIRILRGPGDAVSIEILPVAVATPSGGDAPTPGVPTGSPSPDLAPAAEPAPGAAPPPPPPLPGLPSPQEAPPSAPPQAPPLPGSPEPPPAPPLPGDLPPPPPPPPPPPGTDGPVPPPPPPPPPPPGGPPDALGRRDSELGPGVKAKKPIQTKFRMPLLNWVALKPSQITGTVFTELNDEKVLQELDMSDFEEQFKTKSQGPSLDLSALKSKAAQKAPSKATLIEANRAKNLAITLRKGNLGAERICQAIEAYDLQALGLDFLELLMRFLPTEYERSLITRFEREQRPMEELSEEDRFMLCFSRIPRLPERMTTLTFLGNFPDTAQLLMPQLNAIIAASMSIKSSDKLRQILEIVLAFGNYMNSSKRGAAYGFRLQSLDALLEMKSTDRKQTLLHYLVKVIAEKYPQLTGFHSDLHFLDKAGSVSLDSVLADVRSLQRGLELTQREFVRQDDCMVLKEFLRANSPTMDKLLADSKTAQEAFESVVEYFGENPKTTSPGLFFSLFSRFIKAYKKAEQEVEQWKKEAAAQEAGADTPGKGEPPAPKSPPKARRPQMDLISELKRRQQKEPLIYESDRDGAIEDIITVIKTVPFTARTGKRTSRLLCEASLGEEMPL 91F9CE2B3B526FFC fmnl3_mouse Formin-like protein 3 Fmnl3 Kiaa2014 Frl2 protein protein mouse Mus musculus Mouse MGNLESTDGGPGEPPSVPLLLPPGKTPMPEPCELEERFALVLSSMNLPPDKARLLRQYDNEKKWDLICDQERFQVKNPPHTYIQKLQSFLDPNVTRKKFRRRVQESTKVLRELEISLRTNHIGWVREFLNDENKGLDVLVDYLSFAQCSVMFDFEGLESGDDGAFDKLRSWSRSIEDLQPPNALSAPFTNSLARSARQSVLRYSTLPGRRALKNSRLVSQKDDVHVCILCLRAIMNYQYGFNLVMSHPHAVNEIALSLNNKNPRTKALVLELLAAVCLVRGGHEIILAAFDNFKEVCKELHRFEKLMEYFRNEDSNIDFMVACMQFINIVVHSVEDMNFRVHLQYEFTKLGLEEFLQKSRHTESEKLQVQIQAYLDNVFDVGGLLEDAETKNVALEKVEELEEHVSHLTEKLLDLENENMMRVAELEKQLLQREKELESIKETYENTSNQVHTLRRLIKEKEEAFQRRCHLEPSARGLESMGGEALARVGPTELTEGIPPSDLDLLAPAPPTEETLPLPPPPAPPLPPPPPPLPDKCPPAPPLPGAAPSVVLTVGLSAIRIKKPIKTKFRLPVFNWTALKPNQINGTVFSELDDEKILEDLDLDRFEELFKTKAQGPALDLICSKNKTAQKAASKVTLLEANRAKNLAITLRKAGRSAEEICRAIHTFDLQTLPVDFVECLMRFLPTEAEVKLLRQYERERQPLEELAAEDRFMLLFSKVERLTQRMAGMAFLGNFQDNLQMLTPQLNAIIAASASVKSSQKLKQMLEIILALGNYMNSSKRGAVYGFKLQSLDLLLDTKSTDRKMTLLHFIALTVKEKYPELANFWQELHFVEKAAAVSLENVLLDVKELGRGMELIRRECSIHDNSVLRNFLSTNEGKLDKLQRDAKTAEEAYNAVVRYFGESPKTTPPSVFFPVFVRFIRSYKEAEQENEARKKQEEVMREKQLAQEAKKLDAKTPSQRNKWQQQELIAELRRRQAKEHRPVYEGKDGTIEDIITVLKSVPFTARTAKRGSRFFCDAAHHDESNC BBA0436746934A3B srgp2_human SLIT-ROBO Rho GTPase-activating protein 2 Formin-binding protein 2 SRGAP2 FNBP2 KIAA0456 ARHGAP34 Rho GTPase-activating protein 34 SRGAP2A protein protein human Homo sapiens Human MTSPAKFKKDKEIIAEYDTQVKEIRAQLTEQMKCLDQQCELRVQLLQDLQDFFRKKAEIEMDYSRNLEKLAERFLAKTRSTKDQQFKKDQNVLSPVNCWNLLLNQVKRESRDHTTLSDIYLNNIIPRFVQVSEDSGRLFKKSKEVGQQLQDDLMKVLNELYSVMKTYHMYNADSISAQSKLKEAEKQEEKQIGKSVKQEDRQTPRSPDSTANVRIEEKHVRRSSVKKIEKMKEKRQAKYTENKLKAIKARNEYLLALEATNASVFKYYIHDLSDLIDQCCDLGYHASLNRALRTFLSAELNLEQSKHEGLDAIENAVENLDATSDKQRLMEMYNNVFCPPMKFEFQPHMGDMASQLCAQQPVQSELVQRCQQLQSRLSTLKIENEEVKKTMEATLQTIQDIVTVEDFDVSDCFQYSNSMESVKSTVSETFMSKPSIAKRRANQQETEQFYFTKMKEYLEGRNLITKLQAKHDLLQKTLGESQRTDCSLARRSSTVRKQDSSQAIPLVVESCIRFISRHGLQHEGIFRVSGSQVEVNDIKNAFERGEDPLAGDQNDHDMDSIAGVLKLYFRGLEHPLFPKDIFHDLMACVTMDNLQERALHIRKVLLVLPKTTLIIMRYLFAFLNHLSQFSEENMMDPYNLAICFGPSLMSVPEGHDQVSCQAHVNELIKTIIIQHENIFPSPRELEGPVYSRGGSMEDYCDSPHGETTSVEDSTQDVTAEHHTSDDECEPIEAIAKFDYVGRTARELSFKKGASLLLYQRASDDWWEGRHNGIDGLIPHQYIVVQDTEDGVVERSSPKSEIEVISEPPEEKVTARAGASCPSGGHVADIYLANINKQRKRPESGSIRKTFRSDSHGLSSSLTDSSSPGVGASCRPSSQPIMSQSLPKEGPDKCSISGHGSLNSISRHSSLKNRLDSPQIRKTATAGRSKSFNNHRPMDPEVIAQDIEATMNSALNELRELERQSSVKHTPDVVLDTLEPLKTSPVVAPTSEPSSPLHTQLLKDPEPAFQRSASTAGDIACAFRPVKSVKMAAPVKPPATRPKPTVFPKTNATSPGVNSSTSPQSTDKSCTV DIP protein O75044 original sequence version: 137 5693E6AF33A03E97 fmnl1-srgap2 5 9 unspecified role unspecified role bait bait 11 unspecified role unspecified role prey prey physical association physical association fig 2D srgap2-fmnl3-2 2 7 unspecified role unspecified role prey prey 11 unspecified role unspecified role bait bait 10 unspecified role unspecified role prey prey physical association physical association fig 1A fmnl3-srgap2-1 6 11 unspecified role unspecified role bait bait 10 unspecified role unspecified role prey prey physical association physical association fig 1B fmnl1-srgap2 3 8 unspecified role unspecified role bait bait 10 unspecified role unspecified role bait bait 11 unspecified role unspecified role prey prey direct interaction direct interaction fig 2A fmnl1-srgap2-2 4 11 unspecified role unspecified role prey prey 9 unspecified role unspecified role bait bait physical association physical association fig 2C and 4D srgap2-fmnl1 1 9 unspecified role unspecified role prey prey 11 unspecified role unspecified role bait bait direct interaction direct interaction fig 4C