mpidb The Microbial Protein Interaction Database SepL resembles an aberrant effector in binding to a class 1 type III secretion chaperone and carrying an N-terminal secretion signal. SepL resembles an aberrant effector in binding to a class 1 type III secretion chaperone and carrying an N-terminal secretion signal. J. Bacteriol. (0021-9193) 2010 imex curation Younis R., Bingle L.E., Rollauer S., Munera D., Busby S.J., Johnson S., Deane J.E., Lea S.M., Frankel G., Pallen M.J. 18-Dec-2012: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Younis R., Bingle L.E., Rollauer S., Munera D., Busby S.J., Johnson S., Deane J.E., Lea S.M., Frankel G., Pallen M.J. Accepted 2012-DEC-14 by ORCHARD 18-Dec-2012: Contacted by IntAct-Help. J. Bacteriol. (0021-9193) 2010 Only protein-protein interactions imex curation SepL resembles an aberrant effector in binding to a class 1 type III secretion chaperone and carrying an N-terminal secretion signal. SepL resembles an aberrant effector in binding to a class 1 type III secretion chaperone and carrying an N-terminal secretion signal. J. Bacteriol. (0021-9193) 2010 imex curation Younis R., Bingle L.E., Rollauer S., Munera D., Busby S.J., Johnson S., Deane J.E., Lea S.M., Frankel G., Pallen M.J. 18-Dec-2012: Contacted by IntAct-Help. Only protein-protein interactions eco57 Escherichia coli O157:H7 pull down pull down ms participant Identification by mass spectrometry ms participant Younis R., Bingle L.E., Rollauer S., Munera D., Busby S.J., Johnson S., Deane J.E., Lea S.M., Frankel G., Pallen M.J. Accepted 2012-DEC-14 by ORCHARD 18-Dec-2012: Contacted by IntAct-Help. J. Bacteriol. (0021-9193) 2010 Only protein-protein interactions imex curation SepL resembles an aberrant effector in binding to a class 1 type III secretion chaperone and carrying an N-terminal secretion signal. SepL resembles an aberrant effector in binding to a class 1 type III secretion chaperone and carrying an N-terminal secretion signal. J. Bacteriol. (0021-9193) 2010 imex curation Younis R., Bingle L.E., Rollauer S., Munera D., Busby S.J., Johnson S., Deane J.E., Lea S.M., Frankel G., Pallen M.J. 18-Dec-2012: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro pull down pull down weight by comassie molecular weight estimation by coomasie staining weight by comassie Younis R., Bingle L.E., Rollauer S., Munera D., Busby S.J., Johnson S., Deane J.E., Lea S.M., Frankel G., Pallen M.J. Accepted 2012-DEC-14 by ORCHARD 18-Dec-2012: Contacted by IntAct-Help. J. Bacteriol. (0021-9193) 2010 Only protein-protein interactions imex curation ch60_eco57 Chaperonin GroEL groEL 60 kDa chaperonin Chaperonin-60 groL mopA ECs5124 Z5748 protein protein eco57 Escherichia coli O157:H7 MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSFGAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITEGLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGKLIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESPFILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAVKAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIIDGVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEKKARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAPLRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAASVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM CD3A0FB505F74AD1 q7db69_eco57 cesL E5F08_27450 ECs_4570 mpc protein protein eco57 Escherichia coli O157:H7 MNLLVKRNVEEFLRLLGNDFYLFDNRVEIDFNGFSFFIEIIDNNVFVTFALEYNENAFFSFFSALAPERTQGVIEHIFVYDNKLCLSCLLTNIDVFFLMNTFQQHVQIIERVRRMTS B0FC23C7C15DFD5A q7db65_eco57 E5F08_27470 sepD ECs_4574 protein protein eco57 Escherichia coli O157:H7 MNNNNGIAKNDCDWLTALDFVKDVNGSPTHLTFYIYQKNAFLHDFGNYWVLYIELSGDFRQVPTDTFIRLCNILAVSNEYKQMGIFLSNKKWYLCQIFHKDNNHRANMSKAIMQHTLASLLDKQFDKLEQLSSSDTMMPPTHLFSDIGRIV A11A6F09942B6774 rl1_eco57 50S ribosomal protein L1 rplA Z5557 ECs4907 protein protein eco57 Escherichia coli O157:H7 MAKLTKRMRVIREKVDATKQYDINEAIALLKELATAKFVESVDVAVNLGIDARKSDQNVRGATVLPHGTGRSVRVAVFTQGANAEAAKAAGAELVGMEDLADQIKKGEMNFDVVIASPDAMRVVGQLGQVLGPRGLMPNPKVGTVTPNVAEAVKNAKAGQVRYRNDKNGIIHTTIGKVDFDADKLKENLEALLVALKKAKPTQAKGVYIKKVSISTTMGAGVAVDQAGLSASVN D751704B34748D53 odp1_eco57 Pyruvate dehydrogenase E1 component aceE Z0124 ECs0118 protein protein eco57 Escherichia coli O157:H7 MSERFPNDVDPIETRDWLQAIESVIREEGVERAQYLIDQLLAEARKGGVNVAAGTGISNYINTIPVEEQPEYPGNLELERRIRSAIRWNAIMTVLRASKKDLELGGHMASFQSSATIYDVCFNHFFRARNEQDGGDLVYFQGHISPGVYARAFLEGRLTQEQLDNFRQEVHGNGLSSYPHPKLMPEFWQFPTVSMGLGPIGAIYQAKFLKYLEHRGLKDTSKQTVYAFLGDGEMDEPESKGAITIATREKLDNLVFVINCNLQRLDGPVTGNGKIINELEGIFEGAGWNVIKVMWGSRWDELLRKDTSGKLIQLMNETVDGDYQTFKSKDGAYVREHFFGKYPETAALVADWTDEQIWALNRGGHDPKKIYAAFKKAQETKGKATVILAHTIKGYGMGDAAEGKNIAHQVKKMNMDGVRHIRDRFNVPVSDADIEKLPYITFPEGSEEHTYLHAQRQKLHGYLPSRQPNFTEKLELPSLQDFGALLEEQSKEISTTIAFVRALNVMLKNKSIKDRLVPIIADEARTFGMEGLFRQIGIYSPNGQQYTPQDREQVAYYKEDEKGQILQEGINELGAGCSWLAAATSYSTNNLPMIPFYIYYSMFGFQRIGDLCWAAGDQQARGFLIGGTSGRTTLNGEGLQHEDGHSHIQSLTIPNCISYDPAYAYEVAVIMHDGLERMYGEKQENVYYYITTLNENYHMPAMPEGAEEGIRKGIYKLETIEGSKGKVQLLGSGSILRHVREAAEILAKDYGVGSDVYSVTSFTELARDGQDCERWNMLHPLETPRVPYIAQVMNDAPAVASTDYMKLFAEQVRTYVPADDYRVLGTDGFGRSDSRENLRHHFEVDASYVVVAALGELAKRGEIDKKVVADAIAKFNIDADKVNPRLA 7FB3811DE11BDD02 q7db79_eco57 E5F08_27385 sepL ECs_4557 protein protein eco57 Escherichia coli O157:H7 MANGIEFNQNPASVFNSNSLDFELESQQLTQKNSSNISSPLINLQNELAMITSSSLSETIEGLSLGYRKGSARKEEEGSTIEKLLNDMQELLTLTDSDKIKELSLKNSGLLEQHDPTLAMFGNMPKGEIVALISSLLQSKFVKIELKKKYARLLLDLLGEDDWELALLSWLGVGELNQEGIQKIKKLYEKAKDEDSENGASLLDWFMEIKDLPEREKHLKVIIRALSFDLSYMSSFEDKVKTSSIISDLCRVIIFLSLDNYADIISISIKKDKDIILNEVLSIIEHVWLTEDWLLESPSRVSIVEDKHIYYFHLLKDFFTSLPDACFIDSEQRENALLMIGKVIDYKEEII 0C4A570E183349EC q7db51_eco57 E5F08_27540 ler ECs_4588 protein protein eco57 Escherichia coli O157:H7 MNMENNSHTTSPYIQLIEQIAVLQQEAKRLREQEVQSVIESIQKQITYYNITLQELGYTNVPDDGLARRNSSKGVYYRNEEGQTWSGVGRQPRWLKEALLNGMKKEDFLVKDTEEEIIPLKNI 0C8C70492A83FC37 q7db69-acee-2 2 10 unspecified role unspecified role prey prey 4 unspecified role unspecified role prey prey 8 unspecified role unspecified role prey prey 7 unspecified role unspecified role prey prey 5 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 9 unspecified role unspecified role prey prey 6 unspecified role unspecified role prey prey association association Figure 3 sepl-q7db65 3 6 unspecified role unspecified role prey prey ecolx Escherichia coli 9 unspecified role unspecified role bait bait ecolx Escherichia coli direct interaction direct interaction Fig. 4 sepl-q7db65-1 3 5 unspecified role unspecified role prey prey ecolx Escherichia coli 6 unspecified role unspecified role prey prey ecolx Escherichia coli 9 unspecified role unspecified role bait bait ecolx Escherichia coli association association Fig. 4 q7db65-q7db69 1 5 unspecified role unspecified role neutral component neutral component ecolx Escherichia coli 9 unspecified role unspecified role neutral component neutral component ecolx Escherichia coli 6 unspecified role unspecified role neutral component neutral component ecolx Escherichia coli physical association physical association Fig. 5 q7db65-sepl-1 1 9 unspecified role unspecified role neutral component neutral component ecolx Escherichia coli 6 unspecified role unspecified role neutral component neutral component ecolx Escherichia coli direct interaction direct interaction Fig. 5