mpidb The Microbial Protein Interaction Database The N-terminal domain of Aliivibrio fischeri LuxR is a target of the GroEL chaperonin. The N-terminal domain of Aliivibrio fischeri LuxR is a target of the GroEL chaperonin. J. Bacteriol. (ISSN:0021-9193) 2010 imex curation Manukhov IV, Melkina OE, Goryanin II, Baranova AV, Zavilgelsky GB manukhov@genetika.ru 18-Dec-2012: Contacted by IntAct-Help. Only protein-protein interactions ecolx Escherichia coli affinity chrom affinity chromatography technology Affinity purification Affinity purification affinity chrom ms participant Identification by mass spectrometry ms participant Manukhov IV, Melkina OE, Goryanin II, Baranova AV, Zavilgelsky GB manukhov@genetika.ru Accepted 2012-DEC-14 by ORCHARD 18-Dec-2012: Contacted by IntAct-Help. J. Bacteriol. (ISSN:0021-9193) 2010 Only protein-protein interactions imex curation luxr_alifs Transcriptional activator protein LuxR luxR protein protein alifs alifs Vibrio fischeri MKNINADDTYRIINKIKACRSNNDINQCLSDMTKMVHCEYYLLAIIYPHSMVKSDISILDNYPKKWRQYYDDANLIKYDPIVDYSNSNHSPINWNIFENNAVNKKSPNVIKEAKTSGLITGFSFPIHTANNGFGMLSFAHSEKDNYIDSLFLHACMNIPLIVPSLVDNYRKINIANNKSNNDLTKREKECLAWACEGKSSWDISKILGCSERTVTFHLTNAQMKLNTTNRCQSISKAILTGAIDCPYFKN BDB636192F5E1C64 ch60_ecoli Chaperonin GroEL groEL 60 kDa chaperonin Chaperonin-60 GroEL protein groL mopA JW4103 b4143 protein protein ecoli Escherichia coli (strain K12) MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSFGAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITEGLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGKLIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESPFILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAVKAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIIDGVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEKKARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAPLRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAASVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM CD3A0FB505F74AD1 CD3A0FB505F74AD1 luxr-grol-1 1 3 unspecified role unspecified role prey prey 2 unspecified role unspecified role bait bait region P12746-- gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region P12746-- sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association Figure 3