IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ The Groucho ortholog UNC-37 interacts with the short Groucho-like protein LSY-22 to control developmental decisions in C. elegans. The Groucho ortholog UNC-37 interacts with the short Groucho-like protein LSY-22 to control developmental decisions in C. elegans. Development (0950-1991) 2010 imex curation Flowers E.B., Poole R.J., Tursun B., Bashllari E., Pe'er I., Hobert O. or38@columbia.edu 18-Dec-2012: Contacted by IntAct-Help. Only protein-protein interactions caeel Caenorhabditis elegans anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Flowers E.B., Poole R.J., Tursun B., Bashllari E., Pe'er I., Hobert O. or38@columbia.edu Accepted 2012-NOV-16 by MGT 18-Dec-2012: Contacted by IntAct-Help. Development (0950-1991) 2010 Only protein-protein interactions imex curation The Groucho ortholog UNC-37 interacts with the short Groucho-like protein LSY-22 to control developmental decisions in C. elegans. The Groucho ortholog UNC-37 interacts with the short Groucho-like protein LSY-22 to control developmental decisions in C. elegans. Development (0950-1991) 2010 imex curation Flowers E.B., Poole R.J., Tursun B., Bashllari E., Pe'er I., Hobert O. or38@columbia.edu 18-Dec-2012: Contacted by IntAct-Help. Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Flowers E.B., Poole R.J., Tursun B., Bashllari E., Pe'er I., Hobert O. or38@columbia.edu Accepted 2012-NOV-16 by MGT 18-Dec-2012: Contacted by IntAct-Help. Development (0950-1991) 2010 Only protein-protein interactions imex curation g5ege2_caeel cog-1 CELE_R03C1.3 R03C1.3 protein protein caeel Caenorhabditis elegans MSLIMSEPPQLTTPDDKDVENRKHSSTYSISNLLLEKKESSPSGSSSEDDSASSNDDDQRSPSATSSFIFPPASSSSSSESATSPTQIMAQLMANGGAVDPGLQAYFFLLQSQLNSQSMMNRVTQENVSRALTSFNLLNNLPGALPSLSPMGRLQHSMQLSPNSLNMQKKQSRPTFTGHQIYQLERKFEQTKYLAGADRAQLAQELNMSESQVKVWFQNRRTKWRKKEAADNALVKRGASGDKSPCSLSPFLSAGI E961B929941CC719 unc37_caeel Transcription factor unc-37 unc-37 esg W02D3.9 Uncoordinated protein 37 protein protein caeel Caenorhabditis elegans MKASYLETLDRIKDEHAEMSKHVNQQRSDIEKVALEKENMNRSYMTYAEVSNTLRSDLRKAEEINKRLQEFIMQSLAPQLSQDNQANCLAALEAFKTASPRENGNGAPALPPGFPPGAAGMLGMMPNMPFGMSPAMSQLFNQFASPHVNGGDGAGGSSGGASEAKKAKLEDPDDGELEIDVTNDDHPSTASNGGAANKNGRDSTNSVASSGASTPSIASNSRARQQQQPLAGLQGLEQMNFLAGFNPNLLRQASAAGGFNFLNDPHAQARLAAAIGQIGSRPAYSFKIVDGGVPTPTSFPPDAQKGPGIPTGLKKKMELNHGEVVCAATISRDNSRVYTGGKGCVKIWDVKESDISGATVVNRPPIASLDCLKENYIRSCKLFEDGNTLLIGGEASTVALWDLTTETKTLDLETDSQACYALAMSPDEKLLFACLADGNILIYDIHNKVKVGTLPGHQDGASCLDLSKDGTKLWSGGLDNSVRCWDLAQRKEVAKHDFASQVFSLGCCPNDEWVAVGMENNYVEVLSTTGKEKYQLTQHESCVLSLKFAHSGKFFISTGKDNALNAWRTPYGASLFQLKENSSVLSCDISFDDSLIVTGSGEKKATLYAVEY D3AFF39E526F8249 q93616_caeel CELE_F27D4.2 F27D4.2 lsy-22 protein protein caeel Caenorhabditis elegans MSLADHIDAIKKEMTSLSSQFNSNKSELERSKQDLSSMQLQMQQQMNDSVQQDLQKAHDTSKKLDNLARTFLALITDPSQQSQLLAEFESISKNGTPQMPVMTPPMMNAVAAMQPHMMMRNPMLNAMGGASPRVSGKGGAPQNGGMNGMSMMQHMQMQAFQAQMMQAQMFQQMPMMMPGMVPGMPPGMAGLPGMMPPNNMAAAMQHFNAFQQMAAAASVAVSTPSRNPSTSGAASRTRTPLTTSATNSPRPTTEPTIKEEEPQTMEHDVAAAVAATTATATIKQEENSTVAVA 4865932D2ACC9C8E lsy_22-unc_37 2 4 unspecified role unspecified role prey prey 5 unspecified role unspecified role bait bait physical association physical association Fig. 4A lsy_22-lsy_22 2 5 unspecified role unspecified role bait bait 5 unspecified role unspecified role prey prey physical association physical association Fig. 4A lsy_22-unc_37-1 1 5 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region venus venus fluorescent protein tag venus undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 4 unspecified role unspecified role prey prey physical association physical association Fig. 4A unc_37-cog_1 2 3 unspecified role unspecified role prey prey 4 unspecified role unspecified role bait bait physical association physical association Fig. 4A unc_37-lsy_22-2 2 5 unspecified role unspecified role prey prey 4 unspecified role unspecified role bait bait physical association physical association Fig. 4A