mpidb The Microbial Protein Interaction Database Structure and protein-protein interaction studies on Chlamydia trachomatis protein CT670 (YscO Homolog). Structure and protein-protein interaction studies on Chlamydia trachomatis protein CT670 (YscO Homolog). J. Bacteriol. (0021-9193) 2010 imex curation Lorenzini E., Singer A., Singh B., Lam R., Skarina T., Chirgadze N.Y., Savchenko A., Gupta R.S. Only protein-protein interactions ecolx Escherichia coli solution sedimentati cosedimentation in solution solution sedimentati predetermined predetermined participant predetermined Lorenzini E., Singer A., Singh B., Lam R., Skarina T., Chirgadze N.Y., Savchenko A., Gupta R.S. Accepted 2013-FEB-19 by ORCHARD J. Bacteriol. (0021-9193) 2010 Only protein-protein interactions imex curation Structure and protein-protein interaction studies on Chlamydia trachomatis protein CT670 (YscO Homolog). Structure and protein-protein interaction studies on Chlamydia trachomatis protein CT670 (YscO Homolog). J. Bacteriol. (0021-9193) 2010 imex curation Lorenzini E., Singer A., Singh B., Lam R., Skarina T., Chirgadze N.Y., Savchenko A., Gupta R.S. Only protein-protein interactions ecolx Escherichia coli molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column weight by comassie molecular weight estimation by coomasie staining weight by comassie Lorenzini E., Singer A., Singh B., Lam R., Skarina T., Chirgadze N.Y., Savchenko A., Gupta R.S. Accepted 2013-FEB-19 by ORCHARD J. Bacteriol. (0021-9193) 2010 Only protein-protein interactions imex curation Structure and protein-protein interaction studies on Chlamydia trachomatis protein CT670 (YscO Homolog). Structure and protein-protein interaction studies on Chlamydia trachomatis protein CT670 (YscO Homolog). J. Bacteriol. (0021-9193) 2010 imex curation Lorenzini E., Singer A., Singh B., Lam R., Skarina T., Chirgadze N.Y., Savchenko A., Gupta R.S. Only protein-protein interactions ecolx Escherichia coli pull down pull down western blot western blot Immuno blot Immuno blot Lorenzini E., Singer A., Singh B., Lam R., Skarina T., Chirgadze N.Y., Savchenko A., Gupta R.S. Accepted 2013-FEB-19 by ORCHARD J. Bacteriol. (0021-9193) 2010 Only protein-protein interactions imex curation Structure and protein-protein interaction studies on Chlamydia trachomatis protein CT670 (YscO Homolog). Structure and protein-protein interaction studies on Chlamydia trachomatis protein CT670 (YscO Homolog). J. Bacteriol. (0021-9193) 2010 imex curation Lorenzini E., Singer A., Singh B., Lam R., Skarina T., Chirgadze N.Y., Savchenko A., Gupta R.S. Only protein-protein interactions ecolx Escherichia coli 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Lorenzini E., Singer A., Singh B., Lam R., Skarina T., Chirgadze N.Y., Savchenko A., Gupta R.S. Accepted 2013-FEB-19 by ORCHARD J. Bacteriol. (0021-9193) 2010 Only protein-protein interactions imex curation o84677_chltr CT_670 protein protein chltr chltr MVRYPLEPVLSIKKDRVDRAEKVVKEKRRLLELEQEKLRERESERDKVKNHYMQKIRQLREQLDDGTTSDAILKMKAYIKVVAIQLSEEEEKVNKQKENVLAASKELERAEVELTKRRKEEEKTRLHKEEWMKEALKEEARQEEKEQDEMGQLLHQLHKQKQRESGEN EA85C79076D3024C y671_chltr Uncharacterized protein CT_671 CT_671 protein protein chltr chltr MELNKTSESLFSAKIDHNHPRTEAHEPRDQREVRVFSLEGRSSTRQEKADRMPGRTSSRQESSKGSEEGAVHESTAGVSSKEEEESKGDGFFTGGNPTSGMALVETPMAVVSEAMVETSTMTVSQVDLQWVEQLVTSTVESLLVADIDGKQLVEIVLDNSNTVPAAFCGANLTLVQTGEEISVSFSNFVDQAQLTEATQLVQQNPKQLVSLVESLKARQLNLTELVVGNVAVSLPTIEKIETPLHMIAATIRHHDQEGDQEGEGRQDQHQGQHQEKKVEEAHI F695BEB81FAA6B36 o84677-o84677-2 2 5 unspecified role unspecified role neutral component neutral component n-terminal O84677-- his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 5 unspecified role unspecified role neutral component neutral component n-terminal O84677-- his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Figure 2A o84677-y671 4 5 unspecified role unspecified role bait bait 6 unspecified role unspecified role prey prey physical association physical association Table 3 y671-o84677 3 6 unspecified role unspecified role bait bait n-terminal strep ii tag strep ii tag Strep (II) Strep II Strep (II) Strep II n-term range n-terminal range n-term range n-term range n-terminal range n-term range 5 unspecified role unspecified role prey prey n-terminal O84677-- his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Figure 5 o84677-o84677-3 1 5 unspecified role unspecified role neutral component neutral component n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range 5 unspecified role unspecified role neutral component neutral component n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range physical association physical association Figure 3E and 3F