mbinfo MBInfo http://www.mechanobio.info/ LL5beta directs the translocation of filamin A and SHIP2 to sites of phosphatidylinositol 3,4,5-triphosphate (PtdIns(3,4,5)P3) accumulation, and PtdIns(3,4,5)P3 localization is mutually modified by co-recruited SHIP2. LL5beta directs the translocation of filamin A and SHIP2 to sites of phosphatidylinositol 3,4,5-triphosphate (PtdIns(3,4,5)P3) accumulation, and PtdIns(3,4,5)P3 localization is mutually modified by co-recruited SHIP2. J. Biol. Chem. (0021-9258) 2010 imex curation Takabayashi T., Xie M.J., Takeuchi S., Kawasaki M., Yagi H., Okamoto M., Tariqur R.M., Malik F., Kuroda K., Kubota C., Fujieda S., Nagano T., Sato M. The colocalization of ARNO-PH with LL5-beta (Fig 1C) was not curated since ARNO-PH was mainly used as an indicator of PIP3 localization and did not suggest an interaction. makosato@u-fukui.ac.jp 04-Feb-2013: Contacted by IntAct-Help. Only protein-protein interactions chlae-cos_7 Cercopithecus aethiops simian cells transformed with SV40 Grivet cos_7 cercopithecus aethiops, by transformation with SV40 anti tag coip anti tag coimmunoprecipitation anti tag coip ms participant Identification by mass spectrometry ms participant Takabayashi T., Xie M.J., Takeuchi S., Kawasaki M., Yagi H., Okamoto M., Tariqur R.M., Malik F., Kuroda K., Kubota C., Fujieda S., Nagano T., Sato M. Participant detection: MALDI-TOF mass spectrometry was used to identify filamin A. The colocalization of ARNO-PH with LL5-beta (Fig 1C) was not curated since ARNO-PH was mainly used as an indicator of PIP3 localization and did not suggest an interaction. makosato@u-fukui.ac.jp Accepted 2013-JAN-09 by PPORRAS 04-Feb-2013: Contacted by IntAct-Help. J. Biol. Chem. (0021-9258) 2010 Only protein-protein interactions imex curation LL5beta directs the translocation of filamin A and SHIP2 to sites of phosphatidylinositol 3,4,5-triphosphate (PtdIns(3,4,5)P3) accumulation, and PtdIns(3,4,5)P3 localization is mutually modified by co-recruited SHIP2. LL5beta directs the translocation of filamin A and SHIP2 to sites of phosphatidylinositol 3,4,5-triphosphate (PtdIns(3,4,5)P3) accumulation, and PtdIns(3,4,5)P3 localization is mutually modified by co-recruited SHIP2. J. Biol. Chem. (0021-9258) 2010 imex curation Takabayashi T., Xie M.J., Takeuchi S., Kawasaki M., Yagi H., Okamoto M., Tariqur R.M., Malik F., Kuroda K., Kubota C., Fujieda S., Nagano T., Sato M. The colocalization of ARNO-PH with LL5-beta (Fig 1C) was not curated since ARNO-PH was mainly used as an indicator of PIP3 localization and did not suggest an interaction. makosato@u-fukui.ac.jp 04-Feb-2013: Contacted by IntAct-Help. Only protein-protein interactions chlae-cos_7 Cercopithecus aethiops simian cells transformed with SV40 Grivet cos_7 cercopithecus aethiops, by transformation with SV40 anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Takabayashi T., Xie M.J., Takeuchi S., Kawasaki M., Yagi H., Okamoto M., Tariqur R.M., Malik F., Kuroda K., Kubota C., Fujieda S., Nagano T., Sato M. The colocalization of ARNO-PH with LL5-beta (Fig 1C) was not curated since ARNO-PH was mainly used as an indicator of PIP3 localization and did not suggest an interaction. makosato@u-fukui.ac.jp Accepted 2013-JAN-09 by PPORRAS 04-Feb-2013: Contacted by IntAct-Help. J. Biol. Chem. (0021-9258) 2010 Only protein-protein interactions imex curation LL5beta directs the translocation of filamin A and SHIP2 to sites of phosphatidylinositol 3,4,5-triphosphate (PtdIns(3,4,5)P3) accumulation, and PtdIns(3,4,5)P3 localization is mutually modified by co-recruited SHIP2. LL5beta directs the translocation of filamin A and SHIP2 to sites of phosphatidylinositol 3,4,5-triphosphate (PtdIns(3,4,5)P3) accumulation, and PtdIns(3,4,5)P3 localization is mutually modified by co-recruited SHIP2. J. Biol. Chem. (0021-9258) 2010 imex curation Takabayashi T., Xie M.J., Takeuchi S., Kawasaki M., Yagi H., Okamoto M., Tariqur R.M., Malik F., Kuroda K., Kubota C., Fujieda S., Nagano T., Sato M. The colocalization of ARNO-PH with LL5-beta (Fig 1C) was not curated since ARNO-PH was mainly used as an indicator of PIP3 localization and did not suggest an interaction. makosato@u-fukui.ac.jp 04-Feb-2013: Contacted by IntAct-Help. Only protein-protein interactions chlae-cos_7 Cercopithecus aethiops simian cells transformed with SV40 Grivet cos_7 cercopithecus aethiops, by transformation with SV40 anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Takabayashi T., Xie M.J., Takeuchi S., Kawasaki M., Yagi H., Okamoto M., Tariqur R.M., Malik F., Kuroda K., Kubota C., Fujieda S., Nagano T., Sato M. The colocalization of ARNO-PH with LL5-beta (Fig 1C) was not curated since ARNO-PH was mainly used as an indicator of PIP3 localization and did not suggest an interaction. The colocalization of ARNO-PH with LL5-beta (Fig 1C) was not curated since ARNO-PH was mainly used as an indicator of PIP3 localization and did not suggest an interaction. makosato@u-fukui.ac.jp Accepted 2013-JAN-09 by PPORRAS 04-Feb-2013: Contacted by IntAct-Help. J. Biol. Chem. (0021-9258) 2010 Only protein-protein interactions imex curation LL5beta directs the translocation of filamin A and SHIP2 to sites of phosphatidylinositol 3,4,5-triphosphate (PtdIns(3,4,5)P3) accumulation, and PtdIns(3,4,5)P3 localization is mutually modified by co-recruited SHIP2. LL5beta directs the translocation of filamin A and SHIP2 to sites of phosphatidylinositol 3,4,5-triphosphate (PtdIns(3,4,5)P3) accumulation, and PtdIns(3,4,5)P3 localization is mutually modified by co-recruited SHIP2. J. Biol. Chem. (0021-9258) 2010 imex curation Takabayashi T., Xie M.J., Takeuchi S., Kawasaki M., Yagi H., Okamoto M., Tariqur R.M., Malik F., Kuroda K., Kubota C., Fujieda S., Nagano T., Sato M. The colocalization of ARNO-PH with LL5-beta (Fig 1C) was not curated since ARNO-PH was mainly used as an indicator of PIP3 localization and did not suggest an interaction. makosato@u-fukui.ac.jp 04-Feb-2013: Contacted by IntAct-Help. Only protein-protein interactions chlae-cos_7 Cercopithecus aethiops simian cells transformed with SV40 Grivet cos_7 cercopithecus aethiops, by transformation with SV40 fluorescence imaging fluorescence microscopy fluorescence imaging immunostaining immunostaining Takabayashi T., Xie M.J., Takeuchi S., Kawasaki M., Yagi H., Okamoto M., Tariqur R.M., Malik F., Kuroda K., Kubota C., Fujieda S., Nagano T., Sato M. The colocalization of ARNO-PH with LL5-beta (Fig 1C) was not curated since ARNO-PH was mainly used as an indicator of PIP3 localization and did not suggest an interaction. makosato@u-fukui.ac.jp Accepted 2013-JAN-09 by PPORRAS 04-Feb-2013: Contacted by IntAct-Help. J. Biol. Chem. (0021-9258) 2010 Only protein-protein interactions imex curation phlb2_human Pleckstrin homology-like domain family B member 2 LL5B Protein LL5-beta PHLDB2 protein protein human Homo sapiens Human MEEHSYIQKELDLQNGSLEEDSVVHSVENDSQNMMESLSPKKYSSSLRFKANGDYSGSYLTLSQPVPAKRSPSPLGTSVRSSPSLAKIQGSKQFSYDGTDKNIPMKPPTPLLNTTSSLSGYPLGRADFDHYTGRDSERALRLSEKPPYSKYSSRHKSHDNVYSLGGLEGRKASGSLLAMWNGSSLSDAGPPPISRSGAASMPSSPKQARKMSIQDSLALQPKLTRHKELASENINLRTRKYSSSSLSHMGAYSRSLPRLYRATENQLTPLSLPPRNSLGNSKRTKLGEKDLPHSVIDNDNYLNFSSLSSGALPYKTSASEGNPYVSSTLSVPASPRVARKMLLASTSSCASDDFDQASYVGTNPSHSLLAGESDRVFATRRNFSCGSVEFDEADLESLRQASGTPQPALRERKSSISSISGRDDLMDYHRRQREERLREQEMERLERQRLETILSLCAEYTKPDSRLSTGTTVEDVQKINKELEKLQLSDEESVFEEALMSPDTRYRCHRKDSLPDADLASCGSLSQSSASFFTPRSTRNDELLSDLTRTPPPPSSTFPKASSESSYLSILPKTPEGISEEQRSQELAAMEETRIVILNNLEELKQKIKDINDQMDESFRELDMECALLDGEQKSETTELMKEKEILDHLNRKIAELEKNIVGEKTKEKVKLDAEREKLERLQELYSEQKTQLDNCPESMREQLQQQLKRDADLLDVESKHFEDLEFQQLEHESRLDEEKENLTQQLLREVAEYQRNIVSRKEKISALKKQANHIVQQAQREQDHFVKEKNNLIMMLQREKENLCNLEKKYSSLSGGKGFPVNPNTLKEGYISVNEINEPCGNSTNLSPSTQFPADADAVATEPATAVLASQPQSKEHFRSLEERKKQHKEGLYLSDTLPRKKTTSSISPHFSSATMGRSITPKAHLPLGQSNSCGSVLPPSLAAMAKDSESRRMLRGYNHQQMSEGHRQKSEFYNRTASESNVYLNSFHYPDHSYKDQAFDTLSLDSSDSMETSISACSPDNISSASTSNIARIEEMERLLKQAHAEKTRLLESREREMEAKKRALEEEKRRREILEKRLQEETSQRQKLIEKEVKIRERQRAQARPLTRYLPVRKEDFDLRSHVETAGHNIDTCYHVSITEKTCRGFLIKMGGKIKTWKKRWFVFDRNKRTFSYYADKHETKLKGVIYFQAIEEVYYDHLKNANKSPNPLLTFSVKTHDRIYYMVAPSPEAMRIWMDVIVTGAEGYTHFLL DIP protein Q86SQ0 original sequence version: 61 D0950E96AEE1B020 phlb2_chlae_protein Pleckstrin homology-like domain family B member 2 (chlorocebus) LL5B Protein LL5-beta (chlorocebus) protein protein chlae Chlorocebus aethiops Cercopithecus aethiops Grivet Green monkey flna_human Filamin-A FLNA FLN FLN1 Alpha-filamin Filamin-1 Endothelial actin-binding protein Actin-binding protein 280 Non-muscle filamin protein protein human Homo sapiens Human MSSSHSRAGQSAAGAAPGGGVDTRDAEMPATEKDLAEDAPWKKIQQNTFTRWCNEHLKCVSKRIANLQTDLSDGLRLIALLEVLSQKKMHRKHNQRPTFRQMQLENVSVALEFLDRESIKLVSIDSKAIVDGNLKLILGLIWTLILHYSISMPMWDEEEDEEAKKQTPKQRLLGWIQNKLPQLPITNFSRDWQSGRALGALVDSCAPGLCPDWDSWDASKPVTNAREAMQQADDWLGIPQVITPEEIVDPNVDEHSVMTYLSQFPKAKLKPGAPLRPKLNPKKARAYGPGIEPTGNMVKKRAEFTVETRSAGQGEVLVYVEDPAGHQEEAKVTANNDKNRTFSVWYVPEVTGTHKVTVLFAGQHIAKSPFEVYVDKSQGDASKVTAQGPGLEPSGNIANKTTYFEIFTAGAGTGEVEVVIQDPMGQKGTVEPQLEARGDSTYRCSYQPTMEGVHTVHVTFAGVPIPRSPYTVTVGQACNPSACRAVGRGLQPKGVRVKETADFKVYTKGAGSGELKVTVKGPKGEERVKQKDLGDGVYGFEYYPMVPGTYIVTITWGGQNIGRSPFEVKVGTECGNQKVRAWGPGLEGGVVGKSADFVVEAIGDDVGTLGFSVEGPSQAKIECDDKGDGSCDVRYWPQEAGEYAVHVLCNSEDIRLSPFMADIRDAPQDFHPDRVKARGPGLEKTGVAVNKPAEFTVDAKHGGKAPLRVQVQDNEGCPVEALVKDNGNGTYSCSYVPRKPVKHTAMVSWGGVSIPNSPFRVNVGAGSHPNKVKVYGPGVAKTGLKAHEPTYFTVDCAEAGQGDVSIGIKCAPGVVGPAEADIDFDIIRNDNDTFTVKYTPRGAGSYTIMVLFADQATPTSPIRVKVEPSHDASKVKAEGPGLSRTGVELGKPTHFTVNAKAAGKGKLDVQFSGLTKGDAVRDVDIIDHHDNTYTVKYTPVQQGPVGVNVTYGGDPIPKSPFSVAVSPSLDLSKIKVSGLGEKVDVGKDQEFTVKSKGAGGQGKVASKIVGPSGAAVPCKVEPGLGADNSVVRFLPREEGPYEVEVTYDGVPVPGSPFPLEAVAPTKPSKVKAFGPGLQGGSAGSPARFTIDTKGAGTGGLGLTVEGPCEAQLECLDNGDGTCSVSYVPTEPGDYNINILFADTHIPGSPFKAHVVPCFDASKVKCSGPGLERATAGEVGQFQVDCSSAGSAELTIEICSEAGLPAEVYIQDHGDGTHTITYIPLCPGAYTVTIKYGGQPVPNFPSKLQVEPAVDTSGVQCYGPGIEGQGVFREATTEFSVDARALTQTGGPHVKARVANPSGNLTETYVQDRGDGMYKVEYTPYEEGLHSVDVTYDGSPVPSSPFQVPVTEGCDPSRVRVHGPGIQSGTTNKPNKFTVETRGAGTGGLGLAVEGPSEAKMSCMDNKDGSCSVEYIPYEAGTYSLNVTYGGHQVPGSPFKVPVHDVTDASKVKCSGPGLSPGMVRANLPQSFQVDTSKAGVAPLQVKVQGPKGLVEPVDVVDNADGTQTVNYVPSREGPYSISVLYGDEEVPRSPFKVKVLPTHDASKVKASGPGLNTTGVPASLPVEFTIDAKDAGEGLLAVQITDPEGKPKKTHIQDNHDGTYTVAYVPDVTGRYTILIKYGGDEIPFSPYRVRAVPTGDASKCTVTVSIGGHGLGAGIGPTIQIGEETVITVDTKAAGKGKVTCTVCTPDGSEVDVDVVENEDGTFDIFYTAPQPGKYVICVRFGGEHVPNSPFQVTALAGDQPSVQPPLRSQQLAPQYTYAQGGQQTWAPERPLVGVNGLDVTSLRPFDLVIPFTIKKGEITGEVRMPSGKVAQPTITDNKDGTVTVRYAPSEAGLHEMDIRYDNMHIPGSPLQFYVDYVNCGHVTAYGPGLTHGVVNKPATFTVNTKDAGEGGLSLAIEGPSKAEISCTDNQDGTCSVSYLPVLPGDYSILVKYNEQHVPGSPFTARVTGDDSMRMSHLKVGSAADIPINISETDLSLLTATVVPPSGREEPCLLKRLRNGHVGISFVPKETGEHLVHVKKNGQHVASSPIPVVISQSEIGDASRVRVSGQGLHEGHTFEPAEFIIDTRDAGYGGLSLSIEGPSKVDINTEDLEDGTCRVTYCPTEPGNYIINIKFADQHVPGSPFSVKVTGEGRVKESITRRRRAPSVANVGSHCDLSLKIPEISIQDMTAQVTSPSGKTHEAEIVEGENHTYCIRFVPAEMGTHTVSVKYKGQHVPGSPFQFTVGPLGEGGAHKVRAGGPGLERAEAGVPAEFSIWTREAGAGGLAIAVEGPSKAEISFEDRKDGSCGVAYVVQEPGDYEVSVKFNEEHIPDSPFVVPVASPSGDARRLTVSSLQESGLKVNQPASFAVSLNGAKGAIDAKVHSPSGALEECYVTEIDQDKYAVRFIPRENGVYLIDVKFNGTHIPGSPFKIRVGEPGHGGDPGLVSAYGAGLEGGVTGNPAEFVVNTSNAGAGALSVTIDGPSKVKMDCQECPEGYRVTYTPMAPGSYLISIKYGGPYHIGGSPFKAKVTGPRLVSNHSLHETSSVFVDSLTKATCAPQHGAPGPGPADASKVVAKGLGLSKAYVGQKSSFTVDCSKAGNNMLLVGVHGPRTPCEEILVKHVGSRLYSVSYLLKDKGEYTLVVKWGDEHIPGSPYRVVVP DIP protein P21333 original sequence version: 185 DIP protein P21333-1 original sequence version: 187 DIP protein P21333 original sequence version: 115 DIP protein P21333 original sequence version: 144 DIP protein P21333-1 original sequence version: 156 DIP protein P21333 original sequence version: 201 DIP protein P21333 original sequence version: 197 DIP protein P21333 original sequence version: 170 6C1A07041DF50142 flna_chlae_protein Filamin A (chlorocebus) protein protein chlae Chlorocebus aethiops Cercopithecus aethiops Grivet Green monkey phldb2-flna 2 7 unspecified role unspecified role prey prey n-terminal region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range filamin a(delta3) sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis range range range range range range range range This region includes the actin binding sites 1 and 2, the hinge 2 region and the filamin repeat 24. 5 unspecified role unspecified role bait bait n-terminal region egfp tag enhanced green fluorescent protein tag enhanced green fluorescent protein EGFP enhanced green fluorescent protein EGFP egfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range deltaph region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis range range range range physical association physical association Fig 1E, 1G, 1H, 2B. flna-phldb2 2 5 unspecified role unspecified role prey prey n-terminal region egfp tag enhanced green fluorescent protein tag enhanced green fluorescent protein EGFP enhanced green fluorescent protein EGFP egfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range 7 unspecified role unspecified role bait bait region v5 tag v5 tag GKPIPNPLLGLDST epitope tag GKPIPNPLLGLDST epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined The filamin A constructs were V5-His-tagged. fr24 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain The feature includes the filamin repeat 24, which corresponds to the sequence range [2552-2646] as inferred from uniprot. ch1-ch2 region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis range range range range The feature includes the actin binding regions 1 and 2, which corresponds to sequence range [43-266] as inferred from uniprot. physical association physical association Fig 1F. flna_chlae_pr-phldb2 4 8 unspecified role unspecified role neutral component neutral component 5 unspecified role unspecified role neutral component neutral component n-terminal region egfp tag enhanced green fluorescent protein tag enhanced green fluorescent protein EGFP enhanced green fluorescent protein EGFP egfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range colocalization colocalization Fig 1B (Middle panel), 2E, 2G. EGFP-LL5-beta and endogenous filamin A colocalized at the plasma membrane following EGF stimulation (Fig 1B). EGFP-LL5-beta colocalized with filamin A in a punctate distribution within cytoplasm in cells treated with PI3K inhibitor Wortmannin (Fig 2E). EGFP-LL5-beta-delta PH colocalized with filamin A in the cytoplasm in a punctate manner (Fig 2G). flna_chlae-phlb2_chl 4 6 unspecified role unspecified role neutral component neutral component 8 unspecified role unspecified role neutral component neutral component colocalization colocalization Fig 1B (Right panel). LL5-beta colocalized with filamin A at the plasma membrane in serum-stimulated cells. phldb2-flna_chlae_pr 3 5 unspecified role unspecified role bait bait n-terminal region egfp tag enhanced green fluorescent protein tag enhanced green fluorescent protein EGFP enhanced green fluorescent protein EGFP egfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range deltan2-e required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain 8 unspecified role unspecified role prey prey physical association physical association Fig 1D, 2C. phldb2-flna_chlae_-1 1 8 unspecified role unspecified role prey prey 5 unspecified role unspecified role bait bait n-terminal region egfp tag enhanced green fluorescent protein tag enhanced green fluorescent protein EGFP enhanced green fluorescent protein EGFP egfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range deltan2-e sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association Fig 2D.