mpidb The Microbial Protein Interaction Database Biochemical dissection of the ATPase TraB, the VirB4 homologue of the Escherichia coli pKM101 conjugation machinery. Biochemical dissection of the ATPase TraB, the VirB4 homologue of the Escherichia coli pKM101 conjugation machinery. J. Bacteriol. (0021-9193) 2010 imex curation Durand E., Oomen C., Waksman G. Only protein-protein interactions ecolx Escherichia coli dls dynamic light scattering dls predetermined predetermined participant predetermined Durand E., Oomen C., Waksman G. Accepted 2013-FEB-18 by ORCHARD J. Bacteriol. (0021-9193) 2010 Only protein-protein interactions imex curation Biochemical dissection of the ATPase TraB, the VirB4 homologue of the Escherichia coli pKM101 conjugation machinery. Biochemical dissection of the ATPase TraB, the VirB4 homologue of the Escherichia coli pKM101 conjugation machinery. J. Bacteriol. (0021-9193) 2010 imex curation Durand E., Oomen C., Waksman G. Only protein-protein interactions ecolx Escherichia coli molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Durand E., Oomen C., Waksman G. Accepted 2013-FEB-18 by ORCHARD J. Bacteriol. (0021-9193) 2010 Only protein-protein interactions imex curation Biochemical dissection of the ATPase TraB, the VirB4 homologue of the Escherichia coli pKM101 conjugation machinery. Biochemical dissection of the ATPase TraB, the VirB4 homologue of the Escherichia coli pKM101 conjugation machinery. J. Bacteriol. (0021-9193) 2010 imex curation Durand E., Oomen C., Waksman G. Only protein-protein interactions ecolx Escherichia coli bn-page blue native page bn-page weight by comassie molecular weight estimation by coomasie staining weight by comassie Durand E., Oomen C., Waksman G. Accepted 2013-FEB-18 by ORCHARD J. Bacteriol. (0021-9193) 2010 Only protein-protein interactions imex curation q0zkr5_ecolx traB DNQ45_22565 CDC27_28750 GFY34_23065 GFY34_26265 RCS44_P0031 EIZ93_17590 protein protein ecolx Escherichia coli MRAATATKPKKIDAYRKEPSVNKKYLPYSYHLNDYVISMENGDLMAFFKLDGRTHDCASDRELVTWHKDLNTLVKSFGTDHVELWTHEYHHEAKEYPDGEYDHFFPAYIDQYNRKLHGDSKQLINDLYLTVIYKQVGDKTQKFLAKFEKPTRDEIQQMQNEALEGLEDISEQILEAMKPYGIQQLGIYYRDKRGVEIPAPDKKEREELAEVDESDIFDEAIVIERNEPEPSQAHAYSKALEFLYFLANMEWAIVPVCRDRIREYIMDNRPVSSLWGDVVQIRTVDHNFYTTGIEFREYEEDTEPGQLNMLKEADFEYLLTQSFSCLSESSAKTFLTHQEKSLQETRDRAQSQLAQLGTALDMLTSREFVMGYHHGTVHVWDNDQNAVQRKARRVKVMLTGCGVVGGTLSLASEAAYYARLPGNQKWAPRPVPINSWNFLHFSPFHNFMRGKPDNNPWGPALTMFRTISGTPLYFNFHVTPLEELSYGKRPLGHALITGMSGEGKTTLLNFLLAQSMKYNPRLFVYDRDRGMEPFIRSVGGYYKVLQQGMPSGFAPLQIEPTKRNIALIKNLFRICVETTNNGPISATMATELAEGVDAVMGEGSLIPREARTVTILDGYVNEVVENGVSLKGLLREWTREGQYGWLFDNDKDSLDLSANDIFGFDLSEFIAAKEEVSSPARTPLMMYLLYRVRDSIDGKRRVIQCFDEFHAYLDDPVIEREVKRGIKTDRKKDAIYVFATQEPNDALSSRIGRTIMSQTVTKICLRDPEAIREDYAFLTDAEYDALMSITEHSRQFLVKQGQQSAIASFNLYPRNSDDIDADIKTMDNVLSVLSGEPQNAEIAHELVERLGNDPEVWLKEYWRLTA 35BB5AE5170040BD trab-1 1 4 unspecified role unspecified role neutral component neutral component region Q0ZKR5-1-448 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain Wed Jul 06 16:55:10 BST 2016 Sequence change details about this feature cannot be ascertained or do not fit with the current version of the referenced protein, so they have been deleted as a result of our quality control procedures. The original label was 'region' physical association physical association Table 1 trab-trab-7 1 4 unspecified role unspecified role neutral component neutral component region Q0ZKR5-442-866 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association Table 1 trab-trab-2 3 4 unspecified role unspecified role unspecified role unspecified role region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range physical association physical association Supp. Fig 2C trab-trab-4 3 4 unspecified role unspecified role neutral component neutral component n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range physical association physical association Supplemental figure 2B trab-trab-5 2 4 unspecified role unspecified role unspecified role unspecified role region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association Supplemental figure 2C trab-trab-6 2 4 unspecified role unspecified role unspecified role unspecified role region Q0ZKR5-1-448 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association Supplemental figure 2C