UniProt UniProt http://www.uniprot.org Regulation of mu opioid receptor internalization by the scaffold protein RanBPM. Regulation of mu opioid receptor internalization by the scaffold protein RanBPM. Neurosci. Lett. (0304-3940) 2009 imex curation Talbot J.N., Skifter D.A., Bianchi E., Monaghan D.T., Toews M.L., Murrin L.C. Authors have been contacted to clarify/confirm origin of constructs, but did not reply. The MOP construct used in Y2H exp could not be mapped to UniProt. Origin of FLAG-MOP (fig 2) according UniProt and pmid 10899953 (W. Sadee used HEK293 cells transfected with human FLAG-MOP.) Origin of transfected RanBPM (fig 2) according prey protein in Y2H exp. cmurrin@unmc.edu 18-Dec-2012: Contacted by IntAct-Help. Only protein-protein interactions human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Talbot J.N., Skifter D.A., Bianchi E., Monaghan D.T., Toews M.L., Murrin L.C. Authors have been contacted to clarify/confirm origin of constructs, but did not reply. The MOP construct used in Y2H exp could not be mapped to UniProt. Origin of FLAG-MOP (fig 2) according UniProt and pmid 10899953 (W. Sadee used HEK293 cells transfected with human FLAG-MOP.) Origin of transfected RanBPM (fig 2) according prey protein in Y2H exp. cmurrin@unmc.edu Accepted 2012-NOV-19 by MGT 18-Dec-2012: Contacted by IntAct-Help. Neurosci. Lett. (0304-3940) 2009 Only protein-protein interactions imex curation Regulation of mu opioid receptor internalization by the scaffold protein RanBPM. Regulation of mu opioid receptor internalization by the scaffold protein RanBPM. Neurosci. Lett. (0304-3940) 2009 imex curation Talbot J.N., Skifter D.A., Bianchi E., Monaghan D.T., Toews M.L., Murrin L.C. Authors have been contacted to clarify/confirm origin of constructs, but did not reply. The MOP construct used in Y2H exp could not be mapped to UniProt. Origin of FLAG-MOP (fig 2) according UniProt and pmid 10899953 (W. Sadee used HEK293 cells transfected with human FLAG-MOP.) Origin of transfected RanBPM (fig 2) according prey protein in Y2H exp. cmurrin@unmc.edu 18-Dec-2012: Contacted by IntAct-Help. Only protein-protein interactions human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. anti bait coip anti bait coimmunoprecipitation anti bait coip anti tag western anti tag western blot anti tag western Talbot J.N., Skifter D.A., Bianchi E., Monaghan D.T., Toews M.L., Murrin L.C. Authors have been contacted to clarify/confirm origin of constructs, but did not reply. The MOP construct used in Y2H exp could not be mapped to UniProt. Origin of FLAG-MOP (fig 2) according UniProt and pmid 10899953 (W. Sadee used HEK293 cells transfected with human FLAG-MOP.) Origin of transfected RanBPM (fig 2) according prey protein in Y2H exp. cmurrin@unmc.edu Accepted 2012-NOV-19 by MGT 18-Dec-2012: Contacted by IntAct-Help. Neurosci. Lett. (0304-3940) 2009 Only protein-protein interactions imex curation ranb9_human Ran-binding protein 9 RanBP7 Ran-binding protein M BPM90 BPM-L RANBP9 RANBPM protein protein human Homo sapiens Human MSGQPPPPPPQQQQQQQQLSPPPPAALAPVSGVVLPAPPAVSAGSSPAGSPGGGAGGEGLGAAAAALLLHPPPPPPPATAAPPPPPPPPPPPASAAAPASGPPAPPGLAAGPGPAGGAPTPALVAGSSAAAPFPHGDSALNEQEKELQRRLKRLYPAVDEQETPLPRSWSPKDKFSYIGLSQNNLRVHYKGHGKTPKDAASVRATHPIPAACGIYYFEVKIVSKGRDGYMGIGLSAQGVNMNRLPGWDKHSYGYHGDDGHSFCSSGTGQPYGPTFTTGDVIGCCVNLINNTCFYTKNGHSLGIAFTDLPPNLYPTVGLQTPGEVVDANFGQHPFVFDIEDYMREWRTKIQAQIDRFPIGDREGEWQTMIQKMVSSYLVHHGYCATAEAFARSTDQTVLEELASIKNRQRIQKLVLAGRMGEAIETTQQLYPSLLERNPNLLFTLKVRQFIEMVNGTDSEVRCLGGRSPKSQDSYPVSPRPFSSPSMSPSHGMNIHNLASGKGSTAHFSGFESCSNGVISNKAHQSYCHSNKHQSSNLNVPELNSINMSRSQQVNNFTSNDVDMETDHYSNGVGETSSNGFLNGSSKHDHEMEDCDTEMEVDSSQLRRQLCGGSQAAIERMIHFGRELQAMSEQLRRDCGKNTANKKMLKDAFSLLAYSDPWNSPVGNQLDPIQREPVCSALNSAILETHNLPKQPPLALAMGQATQCLGLMARSGIGSCAFATVEDYLH 50DF1127B7FDA6C8 oprm_human Mu-type opioid receptor OPRM1 MOR1 Mu opioid receptor Mu opiate receptor protein protein human Homo sapiens Human MDSSAAPTNASNCTDALAYSSCSPAPSPGSWVNLSHLDGNLSDPCGPNRTDLGGRDSLCPPTGSPSMITAITIMALYSIVCVVGLFGNFLVMYVIVRYTKMKTATNIYIFNLALADALATSTLPFQSVNYLMGTWPFGTILCKIVISIDYYNMFTSIFTLCTMSVDRYIAVCHPVKALDFRTPRNAKIINVCNWILSSAIGLPVMFMATTKYRQGSIDCTLTFSHPTWYWENLLKICVFIFAFIMPVLIITVCYGLMILRLKSVRMLSGSKEKDRNLRRITRMVLVVVAVFIVCWTPIHIYVIIKALVTIPETTFQTVSWHFCIALGYTNSCLNPVLYAFLDENFKRCFREFCIPTSSNIEQQNSTRIRQNTRDHPSTANTVDRTNHQLENLEAETAPLP 1DABEBEC9AFF6F66 oprm1-ranbp9-2 1 4 unspecified role unspecified role bait bait nucl transfection nucleic acid transfection nucl transfection region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 3 unspecified role unspecified role prey prey nucl transfection nucleic acid transfection nucl transfection physical association physical association 2A and B ranbp9-oprm1 2 4 unspecified role unspecified role prey prey nucl transfection nucleic acid transfection nucl transfection region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 3 unspecified role unspecified role bait bait nucl transfection nucleic acid transfection nucl transfection physical association physical association 2A