mpidb The Microbial Protein Interaction Database Specific interactions between four molybdenum-binding proteins contribute to Mo-dependent gene regulation in Rhodobacter capsulatus. Specific interactions between four molybdenum-binding proteins contribute to Mo-dependent gene regulation in Rhodobacter capsulatus. J. Bacteriol. (0021-9193) 2009 imex curation Wiethaus J., Müller A., Neumann M., Neumann S., Leimkühler S., Narberhaus F., Masepohl B. Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Wiethaus J., Müller A., Neumann M., Neumann S., Leimkühler S., Narberhaus F., Masepohl B. Accepted 2013-FEB-10 by ORCHARD J. Bacteriol. (0021-9193) 2009 Only protein-protein interactions imex curation Specific interactions between four molybdenum-binding proteins contribute to Mo-dependent gene regulation in Rhodobacter capsulatus. Specific interactions between four molybdenum-binding proteins contribute to Mo-dependent gene regulation in Rhodobacter capsulatus. J. Bacteriol. (0021-9193) 2009 imex curation Wiethaus J., Müller A., Neumann M., Neumann S., Leimkühler S., Narberhaus F., Masepohl B. Only protein-protein interactions in vitro In vitro crosslink cross-linking study crosslink anti tag western anti tag western blot anti tag western Wiethaus J., Müller A., Neumann M., Neumann S., Leimkühler S., Narberhaus F., Masepohl B. Accepted 2013-FEB-10 by ORCHARD J. Bacteriol. (0021-9193) 2009 Only protein-protein interactions imex curation Specific interactions between four molybdenum-binding proteins contribute to Mo-dependent gene regulation in Rhodobacter capsulatus. Specific interactions between four molybdenum-binding proteins contribute to Mo-dependent gene regulation in Rhodobacter capsulatus. J. Bacteriol. (0021-9193) 2009 imex curation Wiethaus J., Müller A., Neumann M., Neumann S., Leimkühler S., Narberhaus F., Masepohl B. Only protein-protein interactions ecolx Escherichia coli pull down pull down anti tag western anti tag western blot anti tag western Wiethaus J., Müller A., Neumann M., Neumann S., Leimkühler S., Narberhaus F., Masepohl B. Accepted 2013-FEB-10 by ORCHARD J. Bacteriol. (0021-9193) 2009 Only protein-protein interactions imex curation Specific interactions between four molybdenum-binding proteins contribute to Mo-dependent gene regulation in Rhodobacter capsulatus. Specific interactions between four molybdenum-binding proteins contribute to Mo-dependent gene regulation in Rhodobacter capsulatus. J. Bacteriol. (0021-9193) 2009 imex curation Wiethaus J., Müller A., Neumann M., Neumann S., Leimkühler S., Narberhaus F., Masepohl B. Only protein-protein interactions in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Wiethaus J., Müller A., Neumann M., Neumann S., Leimkühler S., Narberhaus F., Masepohl B. Accepted 2013-FEB-10 by ORCHARD J. Bacteriol. (0021-9193) 2009 Only protein-protein interactions imex curation mopa_rhoca Molybdenum-pterin-binding protein MopA mopA protein protein rhoca Rhodobacter capsulatus Rhodopseudomonas capsulata MNEQPLIAALSLQRAGAPRVGGDRIRLLEAIARHGTIAGAAREVGLSYKTAWDAVGTLNNLFEQPLVEAAPGGRTGGNARVTEAGQALIAGFGLLEGALTKALGVLEGGVSAPEKALNTLWSLTMRTSNRNTLRCTVTRVTLGAVNAEVELALTDGHSLTAVITERSATEMGLAPGVEVFALIKASFVMLAAGGDPGRISACNRLTGIVAARTDGPVNTEIILDLGNCKSITAVITHTSADALGLAPGVPATALFKASHVILAMP 7EB89FC938CAF326 d5arw3_rhocb RCAP_rcc03261 protein protein rhocb Rhodobacter capsulatus (strain ATCC BAA-309 / NBRC 16581 / SB1003) MKLSARNILAGKVTAVETGNVTTHVKIDIGGTVVTASITNEAAADLALKVGDEACAIIKASDVIVGKN 0267E96B01385155 modc_rhoca Molybdenum import ATP-binding protein ModC modC molD protein protein rhoca Rhodobacter capsulatus Rhodopseudomonas capsulata MISARFSGRQGDFTLDAAFDVPGQGVTALFGPSGCGKTTVLRCMAGLTRLPGGHLVVNGVTWQEGRQITPPHRRAVGYVFQEASLFTHLSVRENLVYGLRRARGPLRISEAEVTQLLGIDPLLRRPTATLSGGERQRVAIGRALLSQPELLLMDEPLSALDRISRDEILPYLERLHASLQMPVILVSHDLSEVERLADTLVLMEAGRVRAAGPIAAMQADPNLPLIHRPDLAAVIEGVVIALDPAYGLSTLQVPGGRIVVPGNLGPIGARRRLRVPATDVSLGRHAPTDTTILNALPAVILGAEAAEGYQITVRLALGASGEGASLLARVSRKSFDLLGFQPGEQVVARLKAMALSAPAQTGG 43CD25E9FB7DDB9A mopb_rhoca Molybdenum-pterin-binding protein MopB mopB protein protein rhoca Rhodobacter capsulatus Rhodopseudomonas capsulata MAATKQGGGDDGRCARGVVLERTGARMGAERVALLAAIGRTGSISAAAREVGLSYKAAWDGVQAMNNLLAAPVVTAAPGGKAGGGAVLTPAGEKLIAAYGAIEAGVAKLLSSFEKSLNLDPAEVLRGLSLRTSARNAWACKVWSVAADDVAAQVRMRLGEGQDLTAVITARSAAEMRLAPGSEVLALVKSNFVLLAGAGVPERLSVRNRVRGRVIERIDAPLSSEVTLDLGGGKTITATITRDSAEMLDLHPGVETTALIKSSHVILALP 2FE3B9FC21701156 modc-mopa 1 5 unspecified role unspecified role prey prey 7 unspecified role unspecified role bait bait physical association physical association figure 2 d5arw3-d5arw3 1 6 unspecified role unspecified role prey prey 6 unspecified role unspecified role bait bait physical association physical association figure 2 d5arw3-mopb 1 6 unspecified role unspecified role bait bait 8 unspecified role unspecified role prey prey physical association physical association figure 2 mopb-d5arw3 1 8 unspecified role unspecified role bait bait 6 unspecified role unspecified role prey prey physical association physical association figure 2 mopb-mopb 1 8 unspecified role unspecified role bait bait 8 unspecified role unspecified role prey prey physical association physical association figure 2 mopb-mopa 1 8 unspecified role unspecified role bait bait 5 unspecified role unspecified role prey prey physical association physical association figure 2 mopa-mopb 1 5 unspecified role unspecified role bait bait 8 unspecified role unspecified role prey prey physical association physical association figure 2 mopa-mopa 1 5 unspecified role unspecified role prey prey region Q08385-- sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 5 unspecified role unspecified role bait bait region Q08385-- sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 32 34 physical association physical association figure 2 d5arw3-d5arw3-6 3 6 unspecified role unspecified role prey prey region D5ARW3-- his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 6 unspecified role unspecified role bait bait region D5ARW3-- his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association figure 3 mopb-d5arw3-1 3 6 unspecified role unspecified role prey prey region D5ARW3-- strep ii tag strep ii tag Strep (II) Strep II Strep (II) Strep II undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 8 unspecified role unspecified role bait bait region Q08386-- his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association figure 4 mopb-mopa-1 3 5 unspecified role unspecified role prey prey region Q08385-- strep ii tag strep ii tag Strep (II) Strep II Strep (II) Strep II undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 8 unspecified role unspecified role bait bait region Q08386-- his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association figure 4 mopb-mopb-1 3 8 unspecified role unspecified role prey prey region Q08386-- strep ii tag strep ii tag Strep (II) Strep II Strep (II) Strep II undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 8 unspecified role unspecified role bait bait q08386-- Q08386-- his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association figure 4 mopa-mopb-1 3 8 unspecified role unspecified role prey prey region Q08386-- strep ii tag strep ii tag Strep (II) Strep II Strep (II) Strep II undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 5 unspecified role unspecified role bait bait region Q08385-- his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association figure 4 mopa-mopa-1 3 5 unspecified role unspecified role bait bait region Q08385-- his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 5 unspecified role unspecified role prey prey q08385-- Q08385-- strep ii tag strep ii tag Strep (II) Strep II Strep (II) Strep II undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association figure 4 d5arw3-d5arw3-4 2 6 unspecified role unspecified role neutral component neutral component region D5ARW3-- his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 6 unspecified role unspecified role neutral component neutral component region D5ARW3-- his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined direct interaction direct interaction figure 6 mopb-mopb-4 2 8 unspecified role unspecified role neutral component neutral component region Q08386-- his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecolx Escherichia coli 8 unspecified role unspecified role neutral component neutral component region Q08386-- his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecolx Escherichia coli direct interaction direct interaction figure 6 mopa-mopa-5 2 5 unspecified role unspecified role neutral component neutral component region Q08385-- his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecolx Escherichia coli 5 unspecified role unspecified role neutral component neutral component region Q08385-- his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecolx Escherichia coli direct interaction direct interaction figure 6 mopa-mopa-4 1 5 unspecified role unspecified role bait bait region Q08385-- sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 5 unspecified role unspecified role prey prey region Q08385-- sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 82 84 physical association physical association figure 2, 5 d5arw3-d5arw3-5 4 6 unspecified role unspecified role neutral component neutral component region D5ARW3-- his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 6 unspecified role unspecified role neutral component neutral component region D5ARW3-- his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined direct interaction direct interaction figure 6 mopa-mopa-6 4 5 unspecified role unspecified role neutral component neutral component region Q08385-- his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecolx Escherichia coli direct interaction direct interaction figure 6 mopb-mopb-5 4 8 unspecified role unspecified role neutral component neutral component region Q08386-- his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecolx Escherichia coli direct interaction direct interaction figure 6