UniProt UniProt http://www.uniprot.org Dpr Acts as a molecular switch, inhibiting Wnt signaling when unphosphorylated, but promoting Wnt signaling when phosphorylated by casein kinase Idelta/epsilon. Dpr Acts as a molecular switch, inhibiting Wnt signaling when unphosphorylated, but promoting Wnt signaling when phosphorylated by casein kinase Idelta/epsilon. PLoS ONE 2009 mimix curation Teran E., Branscomb A.D., Seeling J.M. joni.seeling@qc.cuny.edu 19-Sept-2012: Contacted by IntAct-Help. in vitro In vitro anti bait coip anti bait coimmunoprecipitation anti bait coip anti tag western anti tag western blot anti tag western Teran E., Branscomb A.D., Seeling J.M. joni.seeling@qc.cuny.edu Accepted 2012-JUL-31 by ORCHARD 19-Sept-2012: Contacted by IntAct-Help. PLoS ONE 2009 mimix curation Dpr Acts as a molecular switch, inhibiting Wnt signaling when unphosphorylated, but promoting Wnt signaling when phosphorylated by casein kinase Idelta/epsilon. Dpr Acts as a molecular switch, inhibiting Wnt signaling when unphosphorylated, but promoting Wnt signaling when phosphorylated by casein kinase Idelta/epsilon. PLoS ONE 2009 mimix curation Teran E., Branscomb A.D., Seeling J.M. joni.seeling@qc.cuny.edu 19-Sept-2012: Contacted by IntAct-Help. in vitro In vitro in gel kinase assay in-gel kinase assay in gel kinase assay predetermined predetermined participant predetermined Teran E., Branscomb A.D., Seeling J.M. joni.seeling@qc.cuny.edu Accepted 2012-JUL-31 by ORCHARD 19-Sept-2012: Contacted by IntAct-Help. PLoS ONE 2009 mimix curation kc1d_rat Casein kinase I isoform delta Csnk1d Hckid Tau-protein kinase CSNK1D protein protein rat Rattus norvegicus (Rat) Rat MELRVGNRYRLGRKIGSGSFGDIYLGTDIAAGEEVAIKLECVKTKHPQLHIESKIYKMMQGGVGIPTIRWCGAEGDYNVMVMELLGPSLEDLFNFCSRKFSLKTVLLLADQMISRIEYIHSKNFIHRDVKPDNFLMGLGKKGNLVYIIDFGLAKKYRDARTHQHIPYRENKNLTGTARYASINTHLGIEQSRRDDLESLGYVLMYFNLGSLPWQGLKAATKRQKYERISEKKMSTPIEVLCKGYPSEFATYLNFCRSLRFDDKPDYSYLRQLFRNLFHRQGFSYDYVFDWNMLKFGASRAADDAERERRDREERLRHSRNPATRGLPSTASGRLRGTQEVAPPTPLTPTSHTANTSPRPVSGMERERKVSMRLHRGAPVNVSSSDLTGRQDTSRMSTSQIPGRVASSGLQSVVHR B97F04AF9EB466D2 dct1b_xenla Dapper 1-B Dapper1a dact1-b protein protein xenla Xenopus laevis (African clawed frog) African clawed frog MKPIPAAPEPLGQHQDSPRRKDKGEAESERQRTRERLEATLAGLAELGHLRHRQEVLIKSVLSPGTRTHGDAAARTGDNPRSLEEKFLEDNILLLKKQLNCLRKRDAGLLSQLHELDKQINDLRIDVEKTEEHLETDSRPSSGFYELSDGTSGSLSNSSNSVFSECLSSCHSSTCFCNPLETSLNLTDGQAKSADDFLEWLDYRESQHETGTVRRSFSAPHSNSVDIEADVHPKYQCDLVSKNGNDIYRYPSPLHAVAVQSPMFLLSVMGNIKAEEPEEGIDHNDNDDCIVPELDHLKDEDSFLHQSSLCSLPLSSAKKMDGYILSIIQKKAHPVRTNKPRTSVNADPGKGILRHGSMCVKQTGGVSQSNAVNLKNSKQTCLHSTGMIAVDNSTYPSLKQCSKESLSEQLESKRMPSISTYPSCNVNELQSQNNSRNTVKSVCQGLARGSVAMTSNVQKENVTPNALANLSNTSSSVCNVTPGESMQNSPLLPQEIKVVPPVKRVSPQNTLLSYHASSSFDERPPLDFKSEGSSSQSLDEGLLVNAHYIPAQQQGVKLHKHTKYVKIVKSSTLKHRANVQYVAENGSQTLKEKSKVVGKKCRFPDDLDTNKKVKKSTLRVKKTAHPHFEPAVVGRNPVAVRSGSKSHGHSKDVVLAKPKHKRGDYRRWKSSAEISYEEALRRARRRAQGEMVGVYAQVPFPYSSPYAYIASDSEYSAECESLFHSTVVDTSEDEQSNYTTNCFGDSESSLSEVEFVGESTTSSDTDESGGLIWSQFVQTLPMQATATAELQTTAKAFVKIKASHNLKKKILRFRSGSLKLMTTV F2172CD999DF7814 dvl2_xenla Segment polarity protein dishevelled homolog DVL-2 dvl2 dsh DSH homolog 2 Xdsh protein protein xenla Xenopus laevis (African clawed frog) African clawed frog MAETKVIYHLDEEETPYLVKVPVPATDIRLRDFKAALGRGHAKYFFKAMDQDFGVVKEEISDDNAKLPCFNDRVVSWLASSEGSQPDSAPPAPATEVRPEPPPPVPPPIPPPPAERTSGIGDSRPPSFHPNVSGSTEQLDQDNESVISMRRDRVRRRESSEQAGVGRGVNGRTERHLSGYESSSTLLTSEIETSICDSEEDDTMSRFSSSTEQSSASRLLKRHRRRRKQRPPRLERTSSFSSVTDSTMSLNIITVTLNMEKYNFLGISIVGQSNERGDGGIYIGSIMKGGAVAADGRIEPGDMLLQVNDINFENMSNDDAVRVLRDIVHKPGPIVLTVAKCWDPSPQGYFTLPRNEPIHPIDPAAWVSHSAALSGSFPVYPGSASMSSMTSSTSVTETELSHALPPVSLFSLSVHTDLASVVKVMASPESGLEVRDRMWLKITIPNAFLGSDVVDWLYHHVEGFQDRREARKFASNLLKAGFIRHTVNKITFSEQCYYIFGDLTGCENYMTNLSLNDNDGSSGASDQDTLAPLPLPGASPWPLLPTFSYQYQAPHPYSTQPPAYHELSSYSYGMGSAGSQHSEGSRSSGSNRSDGGRGMQKDDRSGVAGVGGGDSKSGSGSESEYSTRSSIRRVGGGEAGPPSERSTSSRLPPHHPPSVHSYAAPGVPLSYNPMMLMMMPPPPLPPPGVCPPNSSVPPGAPPLVRDLASVPPELTATRQSFHMAMGNPSEFFVDVM AF6C9A1662DD7CEB csnk1d-dact1_b-1 2 4 enzyme target enzyme target substrate substrate neutral component neutral component rabit-reticulocyte Oryctolagus cuniculus reticulocyte reticulocyte Reticulocytes 5 ancillary ancillary neutral component neutral component 3 enzyme enzyme neutral component neutral component region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined in vitro In vitro Rat CKIalpha lacking its autoinhibtory domain was used (cannot not be mapped to UniProt. phosphorylation phosphorylation reaction phosphorylation 2A dact1_b-dvl2 1 4 unspecified role unspecified role bait bait region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 5 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association 4A CKI-delta