mpidb The Microbial Protein Interaction Database Allosteric regulation of the primase (DnaG) activity by the clamp-loader (tau) in vitro. Allosteric regulation of the primase (DnaG) activity by the clamp-loader (tau) in vitro. Mol. Microbiol. (0950-382X) 2009 imex curation Chintakayala K., Machón C., Haroniti A., Larson M.A., Hinrichs S.H., Griep M.A., Soultanas P. Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Chintakayala K., Machón C., Haroniti A., Larson M.A., Hinrichs S.H., Griep M.A., Soultanas P. Accepted 2013-FEB-12 by ORCHARD Mol. Microbiol. (0950-382X) 2009 Only protein-protein interactions imex curation Allosteric regulation of the primase (DnaG) activity by the clamp-loader (tau) in vitro. Allosteric regulation of the primase (DnaG) activity by the clamp-loader (tau) in vitro. Mol. Microbiol. (0950-382X) 2009 imex curation Chintakayala K., Machón C., Haroniti A., Larson M.A., Hinrichs S.H., Griep M.A., Soultanas P. Only protein-protein interactions in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Chintakayala K., Machón C., Haroniti A., Larson M.A., Hinrichs S.H., Griep M.A., Soultanas P. Accepted 2013-FEB-12 by ORCHARD Mol. Microbiol. (0950-382X) 2009 Only protein-protein interactions imex curation dpo3x_bacsu DNA polymerase III subunit gamma/tau dnaH BSU00190 dnaX protein protein bacsu Bacillus subtilis MSYQALYRVFRPQRFEDVVGQEHITKTLQNALLQKKFSHAYLFSGPRGTGKTSAAKIFAKAVNCEHAPVDEPCNECAACKGITNGSISDVIEIDAASNNGVDEIRDIRDKVKFAPSAVTYKVYIIDEVHMLSIGAFNALLKTLEEPPEHCIFILATTEPHKIPLTIISRCQRFDFKRITSQAIVGRMNKIVDAEQLQVEEGSLEIIASAADGGMRDALSLLDQAISFSGDILKVEDALLITGAVSQLYIGKLAKSLHDKNVSDALETLNELLQQGKDPAKLIEDMIFYFRDMLLYKTAPGLEGVLEKVKVDETFRELSEQIPAQALYEMIDILNKSHQEMKWTNHPRIFFEVAVVKICQTSHQSAADLPEVDMLMKKIQQLEQEVERLKTTGIKAAAESPKKEAPRVPKGGKSNYKAPVGRIHEILKEATRPDLDLLRNSWGKLLAHLKQQNKVSHAALLNDSEPVAAGSAAFVLKFKYEIHCKMVAEDNNGVRTNLEQILESMLGKRMDLIGVPEAQWGKIREEFLEDHQQENEGSNEPAEEDPLIAEAKKLVGADLIEIKD F5975C246622E4F5 q9x4c9_geose Replicative DNA helicase dnaB protein protein geose Geobacillus stearothermophilus Geobacillus stearothermophilus Bacillus stearothermophilus MSELFSERIPPQSIEAEQAVLGAVFLDPAALVPASEILIPEDFYRAAHQKIFHAMLRVADRGEPVDLVTVTAELAASEQLEEIGGVSYLSELADAVPTAANVEYYARIVEEKSVLRRLIRTATSIAQDGYTREDEIDVLLDEADRKIMEVSQRKHSGAFKNIKDILVQTYDNIEMLHNRDGEITGIPTGFTELDRMTSGFQRSDLIIVAARPSVGKTAFALNIAQNVATKTNENVAIFSLEMSAQQLVMRMLCAEGNINAQNLRTGKLTPEDWGKLTMAMGSLSNAGIYIDDTPSIRVSDIRAKCRRLKQESGLGMIVIDYLQLIQGSGRSKENRQQEVSEISRSLKALARELEVPVIALSQLSRSVEQRQDKRPMMSDIRESGSIEQDADIVAFLYRDDYYNKDSENKNIIEIIIAKQRNGPVGTVQLAFIKEYNKFVNLERRFDEAQIPPGA DIP protein Q9X4C9 original sequence version: 62 D6115B868E11BAC0 dnag_geose DNA primase dnaG protein protein geose Geobacillus stearothermophilus Geobacillus stearothermophilus Bacillus stearothermophilus MGHRIPEETIEAIRRGVDIVDVIGEYVQLKRQGRNYFGLCPFHGEKTPSFSVSPEKQIFHCFGCGAGGNAFTFLMDIEGIPFVEAAKRLAAKAGVDLSVYELDVRGRDDGQTDEAKAMTEAHALLKRFYHHLLVHTKEGQAALDYLQARGWTKETIDRFEIGYAPDAPDAAAKLLESHSFSLPVMEKAGLLTKKEDGRYVGRFRNRIMFPIHDHRGETVGFSGRLLGEGHPKYVNSPETPVFRKGAILYHFHAARVPIRKRQEALLVEGFADVISAAQAGIDYAIATMGTSLTEEQARILRPCDTITICYDGDRAGIEAAWAAAEQLSALGCRVKVASLPNGLDPDEYIRVYGGERFAGEAGCRRPLVAFKMAYLRRGKNLQHEGERLRYIDEALREIGKLSSPVEQDYYLRQLAEEFSLSLSALHEQLSRSQRERTKPREAPDGETARPMLAKKLLPAFQNAERLLLAHMMRSRDVALVVQERIGGRFNIEEHRALAAYIYAFYEEGHEADPGALISRIPGELQPLASDVSLLLIADDVSEQELEDYIRHVLNRPKWLMLKVKEQEKTEAERRKDFLTAARIAKEMIEMKKMLSSS DIP protein Q9X4D0 original sequence version: 74 02FA690CB6798447 dnab-dnag-1 2 4 unspecified role unspecified role neutral component neutral component 5 unspecified role unspecified role neutral component neutral component physical association physical association Fig. 1F dnab-dnag-1 2 3 unspecified role unspecified role neutral component neutral component 4 unspecified role unspecified role neutral component neutral component 5 unspecified role unspecified role neutral component neutral component physical association physical association Fig. 1B dnax-dnab 1 3 unspecified role unspecified role bait bait P09122:p.Ala550Val mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain A V Wed May 02 16:16:35 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ala550val' Tue Jun 30 17:12:27 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ala550Val' P09122:p.Asp510Ser mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain D S Wed May 02 19:16:27 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asp510ser' Tue Jun 30 20:19:52 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asp510Ser' P09122:p.Gly492Arg mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain G R Wed May 02 20:10:06 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gly492arg' Tue Jun 30 19:23:57 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly492Arg' P09122:p.Trp519Arg mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain W R Wed May 02 14:40:49 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'trp519arg' Tue Jun 30 16:27:11 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Trp519Arg' P09122:p.Phe473Leu mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain F L Wed May 02 16:28:21 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'phe473leu' Tue Jun 30 18:27:26 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Phe473Leu' P09122:p.Cys483Ser mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain C S Wed May 02 15:14:57 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'cys483ser' Tue Jun 30 18:26:14 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Cys483Ser' 4 unspecified role unspecified role prey prey physical association physical association Fig. 3