UniProt UniProt http://www.uniprot.org Arabidopsis protein kinases GRIK1 and GRIK2 specifically activate SnRK1 by phosphorylating its activation loop. Arabidopsis protein kinases GRIK1 and GRIK2 specifically activate SnRK1 by phosphorylating its activation loop. Plant Physiol. (0032-0889) 2009 imex curation Shen W., Reyes M.I., Hanley-Bowdoin L. wshen@ncsu.edu 18-Dec-2012: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro protein kinase assay protein kinase assay weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Shen W., Reyes M.I., Hanley-Bowdoin L. wshen@ncsu.edu Accepted 2012-DEC-14 by ORCHARD 18-Dec-2012: Contacted by IntAct-Help. Plant Physiol. (0032-0889) 2009 Only protein-protein interactions imex curation Arabidopsis protein kinases GRIK1 and GRIK2 specifically activate SnRK1 by phosphorylating its activation loop. Arabidopsis protein kinases GRIK1 and GRIK2 specifically activate SnRK1 by phosphorylating its activation loop. Plant Physiol. (0032-0889) 2009 imex curation Shen W., Reyes M.I., Hanley-Bowdoin L. wshen@ncsu.edu 18-Dec-2012: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Shen W., Reyes M.I., Hanley-Bowdoin L. wshen@ncsu.edu Accepted 2012-DEC-14 by ORCHARD 18-Dec-2012: Contacted by IntAct-Help. Plant Physiol. (0032-0889) 2009 Only protein-protein interactions imex curation grik1_arath Serine/threonine-protein kinase GRIK1 Protein GEMINIVIRUS REP INTERACTING KINASE 1 SNAK2 SnRK1-activating protein kinase 2 F18N11 T14D3.180 At3g45240 GRIK1 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MFCDSFAFAQVMSCFGCFGGSERSRHSPNPYDDDTYSHDSGETSNPGGDDEEGEEEEEVEELSRSKRSEEILKCKLQNGLVCRQFPVKETNKLTRGEDEDGNKTINEFVRERKIGSGSYGKVVLYRSTVDDKHYAIKAFHKSHLSRLRVAPSETAMGDVLREVMIMKTLEHPNIVNLIEVIDDPEFDDFYMVLEYVDGKWAYDDSGPPGALGEITARKYLRDVVAGLMYLHAHNVIHGDIKPDNLLVTSTGRVKIGDFSVSQVFKDDDDQLRRSPGTPVFTAPECCLGITYSGRSADTWAVGVTLYCMILGQYPFLGDTLQDTYDKIVHNPLIIPEGLNPRLRDLIEGLLCKDPNQRMTLKAVAEHPWITGEDGAISEYCCWCKRKAEEEEDQNHS F3C56641D73006B4 grik2_arath Serine/threonine-protein kinase GRIK2 GRIK2 Protein GEMINIVIRUS REP INTERACTING KINASE 2 SNAK1 SnRK1-activating protein kinase 1 MUF9.18 At5g60550 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MFRDSFLFARTIGCFGCFGSSGSRNQQSPKPYDDDTHSCDSDVTSTARGEEEEDEEEVEQKSRSKRSEEILKYRLDNGLICRHIPVRETNELIRGEDENGDKTINEYVRVCKIGSGSYGKVVLYRSTLDGQYYAIKAFHKSHLLRLRVAPSETAMSDVLREVMIMKILEHPNIVNLIEVIDDPETDHFYMVLEYVDGKWVYDGSGPPGALGEKTARKYLRDIVTGLMYLHAHDVIHGDIKPDNLLVTSSGTVKIGDFSVSQVFKDDDDQLRRSPGTPVFTAPECCLVSGITYSGRAADTWAVGVTLYCMILGQYPFLADTLQDTYDKIVNNPLIIPDGLNPLLRDLIEGLLCKDPSQRMTLKNVSEHPWVIGEDGHVPEYFCWCKRNAASKIEEGEANGISETSDPN FD7832EE2990BB11 kin11_arath SNF1-related protein kinase catalytic subunit alpha KIN11 KIN11 AKIN alpha-1 AKIN11 SNF1-related kinase 1.2 SNR1 SNRK1.2 MXE2.16 At3g29160 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MDHSSNRFGNNGVESILPNYKLGKTLGIGSFGKVKIAEHVVTGHKVAIKILNRRKIKNMEMEEKVRREIKILRLFMHPHIIRQYEVIETTSDIYVVMEYVKSGELFDYIVEKGRLQEDEARNFFQQIISGVEYCHRNMVVHRDLKPENLLLDSRCNIKIADFGLSNVMRDGHFLKTSCGSPNYAAPEVISGKLYAGPEVDVWSCGVILYALLCGTLPFDDENIPNLFKKIKGGIYTLPSHLSSEARDLIPRMLIVDPVKRITIPEIRQHRWFQTHLPRYLAVSPPDTVEQAKKINEEIVQEVVNMGFDRNQVLESLRNRTQNDATVTYYLLLDNRFRVPSGYLESEFQETTDSGSNPMRTPEAGASPVGHWIPAHVDHYGLGARSQVPVDRKWALGLQSHAHPREIMNEVLKALQELNVCWKKIGHYNMKCRWVPGLADGQNTMVNNQLHFRDESSIIEDDCAMTSPTVIKFELQLYKAREEKYLLDIQRVNGPQFLFLDLCAAFLTELRVI 4FBEF55D3EC60F87 q38997-2 SNF1-related protein kinase catalytic subunit alpha KIN10 KIN10 AK21 AKIN10 SKIN10 SNR2 SNRK1.1 T4P13.22 At3g01090 AKIN alpha-2 SNF1-related kinase 1.1 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MDGSGTGSRSGVESILPNYKLGRTLGIGSFGRVKIAEHALTGHKVAIKILNRRKIKNMEMEEKVRREIKILRLFMHPHIIRLYEVIETPTDIYLVMEYVNSGELFDYIVEKGRLQEDEARNFFQQIISGVEYCHRNMVVHRDLKPENLLLDSKCNVKIADFGLSNIMRDGHFLKTSCGSPNYAAPEVISGKLYAGPEVDVWSCGVILYALLCGTLPFDDENIPNLFKKIKGGIYTLPSHLSPGARDLIPRMLVVDPMKRVTIPEIRQHPWFQAHLPRYLAVPPPDTVQQAKKIDEEILQEVINMGFDRNHLIESLRNRTQNDGTVTYYLILDNRFRASSGYLGAEFQETMEGTPRMHPAESVASPVSHRLPGLMEYQGVGLRSQYPVERKWALGLQSRAHPREIMTEVLKALQDLNVCWKKIGHYNMKCRWVPNSSADGMLSNSMHDNNYFGDESSIIENEAAVKSPNVVKFEIQLYKTRDDKYLLDLQRVQGPQFLFLDLCAAFLAQLRVL 5A18655A0AA506DF grik1-kin10 2 6 unspecified role unspecified role prey prey region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined kd activation domain activation domain tag activation domain certain certain sequence position certain certain certain sequence position certain in vitro In vitro 3 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Q93V58:p.Lys137Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain K A Wed May 02 16:36:31 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys137ala' Tue Jun 30 16:42:57 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys137Ala' in vitro In vitro physical association physical association Fig. 3 grik2-kin10 2 6 unspecified role unspecified role prey prey region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined kd activation domain activation domain tag activation domain certain certain sequence position certain certain certain sequence position certain in vitro In vitro 4 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Q5HZ38:p.Lys136Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain K A Wed May 02 15:10:04 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys136ala' Tue Jun 30 17:46:22 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys136Ala' in vitro In vitro physical association physical association Fig. 3 grik1-grik1-1 1 3 putative self putative self putative self putative self Q93V58:p.Lys137Ala mutation disrupting rate mutation disrupting interaction rate mutation disrupting rate certain certain sequence position certain certain certain sequence position certain K A Wed May 02 20:19:00 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys137ala' Tue Jun 30 17:31:38 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys137Ala' region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) phosphorylation phosphorylation reaction phosphorylation true Fig. 1A, FIg.4A grik1-kin10-1 1 3 enzyme enzyme unspecified role unspecified role Q93V58:p.Lys137Ala mutation disrupting rate mutation disrupting interaction rate mutation disrupting rate certain certain sequence position certain certain certain sequence position certain K A Wed May 02 16:21:55 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys137ala' Tue Jun 30 17:04:43 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys137Ala' region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 6 enzyme target enzyme target substrate substrate unspecified role unspecified role kd activation domain activation domain tag activation domain certain certain sequence position certain certain certain sequence position certain Q38997-2:p.Thr175Ala mutation disrupting rate mutation disrupting interaction rate mutation disrupting rate certain certain sequence position certain certain certain sequence position certain T A Wed May 02 17:07:07 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'thr198ala' Tue Jun 30 18:04:55 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Thr175Ala' Q38997-2:p.Lys48Ala mutation mutation certain certain sequence position certain certain certain sequence position certain K A Wed May 02 18:11:44 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys71ala' Tue Jun 30 16:54:40 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys48Ala' region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) phosphorylation phosphorylation reaction phosphorylation Fig. 1A, Fig. 4A grik1-kin11 1 5 enzyme target enzyme target substrate substrate unspecified role unspecified role kd activation domain activation domain tag activation domain certain certain sequence position certain certain certain sequence position certain region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined P92958:p.Lys49Ala mutation mutation certain certain sequence position certain certain certain sequence position certain K A Wed May 02 15:56:50 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys49ala' Tue Jun 30 17:00:44 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys49Ala' ecoli Escherichia coli (strain K12) 3 enzyme enzyme unspecified role unspecified role Q93V58:p.Lys137Ala mutation disrupting rate mutation disrupting interaction rate mutation disrupting rate certain certain sequence position certain certain certain sequence position certain K A Wed May 02 19:32:45 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys137ala' Tue Jun 30 17:23:36 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys137Ala' region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) phosphorylation phosphorylation reaction phosphorylation Fig. 1A snak1-snak1 1 4 putative self putative self putative self putative self region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Q5HZ38:p.Lys136Ala mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate certain certain sequence position certain certain certain sequence position certain K A Wed May 02 16:46:24 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys136ala' Tue Jun 30 20:16:07 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys136Ala' ecoli Escherichia coli (strain K12) phosphorylation phosphorylation reaction phosphorylation true Fig. 1B, Fig. 4B grik2-kin10 1 6 enzyme target enzyme target substrate substrate unspecified role unspecified role region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Q38997-2:p.Lys48Ala mutation mutation certain certain sequence position certain certain certain sequence position certain K A Wed May 02 15:22:16 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys71ala' Tue Jun 30 16:08:05 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys48Ala' Q38997-2:p.Thr175Ala mutation disrupting rate mutation disrupting interaction rate mutation disrupting rate certain certain sequence position certain certain certain sequence position certain T A Wed May 02 14:31:08 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'thr198ala' Tue Jun 30 18:48:25 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Thr175Ala' kd activation domain activation domain tag activation domain certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 4 enzyme enzyme unspecified role unspecified role region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Q5HZ38:p.Lys136Ala mutation disrupting rate mutation disrupting interaction rate mutation disrupting rate certain certain sequence position certain certain certain sequence position certain K A Wed May 02 17:31:09 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys136ala' Tue Jun 30 19:11:45 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys136Ala' ecoli Escherichia coli (strain K12) phosphorylation phosphorylation reaction phosphorylation Fig. 1B, Fig. 4B grik2-kin11 1 5 enzyme target enzyme target substrate substrate unspecified role unspecified role P92958:p.Lys49Ala mutation mutation certain certain sequence position certain certain certain sequence position certain K A Wed May 02 18:28:57 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys49ala' Tue Jun 30 19:44:33 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys49Ala' kd activation domain activation domain tag activation domain certain certain sequence position certain certain certain sequence position certain region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 4 enzyme enzyme unspecified role unspecified role region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Q5HZ38:p.Lys136Ala mutation disrupting rate mutation disrupting interaction rate mutation disrupting rate certain certain sequence position certain certain certain sequence position certain K A Wed May 02 18:17:44 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys136ala' Tue Jun 30 18:02:14 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys136Ala' ecoli Escherichia coli (strain K12) phosphorylation phosphorylation reaction phosphorylation Fig. 1B