mpidb The Microbial Protein Interaction Database Agrobacterium VirB10 domain requirements for type IV secretion and T pilus biogenesis. Agrobacterium VirB10 domain requirements for type IV secretion and T pilus biogenesis. Mol. Microbiol. (0950-382X) 2009 imex curation Jakubowski S.J., Kerr J.E., Garza I., Krishnamoorthy V., Bayliss R., Waksman G., Christie P.J. 04-Feb-2013: Contacted by IntAct-Help. Only protein-protein interactions rhird Rhizobium radiobacter (Agrobacterium tumefaciens) Agrobacterium radiobacter Agrobacterium tumefaciens anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Jakubowski S.J., Kerr J.E., Garza I., Krishnamoorthy V., Bayliss R., Waksman G., Christie P.J. Accepted 2013-JAN-18 by ORCHARD 04-Feb-2013: Contacted by IntAct-Help. Mol. Microbiol. (0950-382X) 2009 Only protein-protein interactions imex curation vird4_rhird Protein VirD4 virD4 protein protein rhird Rhizobium radiobacter (Agrobacterium tumefaciens) Agrobacterium radiobacter Agrobacterium tumefaciens MNSSKTSPQRMTLSIVCSLAAGFCAASCYVTFRRGFNGEAIMTFDVFAFWYETPLYLGYASTVFWRGLSVVIFTSLAVLSSQLIISLRNQKHHGTARWAEIGEMQHAGYLQRYSRIKGPIFGKTCGPLWFGSYLTNGEQPHSLVVAPTRAGKGVGIVIPTLLTFKGSVIALDVKGELFELTSRARKASGDAVFKFSPLDSERKTHCYNPVLDIATLPPERQFTETRRLAANLITAKGKGAEGFIDGARDLFVAGILTCIERGTPTIGAVYDLFAQPGEKYKLFAQLAEESLNKEAQRIFDNMAGNDTKILTSYTSVLGDGGLNLWADPLIKAATSRSDFSVYDLRRKKTCIYLCVSPNDLEVLAPLMRLLFQQLVSILQRSLPGKDECHEVLFLLDEFKHLGKLEAIETAITTIAGYKGRFMFIIQSLSALTGTYDDAGKQNFLSNTGVQVFMATADDETPTYISKAIGEYTFKARSTSYSQASMFDHNIQISDQGAALLRPEQVRLLDDQSEIVLIKGRPPLKLRKVQYYSDRTLKRLFERQMGSLPEPAPLMLSDYSNDQVQSHLAEIANFNEDAAPRNRTVAEDHGSVKVGADIPERVMGINGDEDQADAREIPPELGCASRIDAGSGRSTAIVGPDYCTSAKIEVRTGTACEMKLNWVQFVSGGMLRFDKVYARSQLSLYEVSV DIP protein P09817 original sequence version: 49 43BF81126FFC3C46 virba_rhird Protein virB10 virB10 protein protein rhird Rhizobium radiobacter (Agrobacterium tumefaciens) Agrobacterium radiobacter Agrobacterium tumefaciens MNNDSQQAAHEVDASGSLVSDKHRRRLSGSQKLIVGGVVLALSLSLIWLGGRQKKVNDNASPSTLIAANTKPFHPAPIEVPPDTPAVQEAVQPTVPQPPRGEPERMSHGRKKHRFLHIAVAIKGSASAPVRATWAEDKKTSVTTTPCRMRSVRRERFVDTYETHRAAAQQGTLLPHPDFMVTQGTIIPCILQTAIDTNLAGYVKCVLPQDIRGTTNNIVLLDRGTTVVGEIQRGLQQGDERVFVLWDRAETPDHAMISLTSPSADELGRPGLPGSVDSHFWQRFSGAMLLSAVQGAFQAASTYAGSSGGGMSFNSFQNNGEQTTETALKATINIPPTLKKNQGDTVSIFVARDLDFFGVYQLRLTGGAARGRNRRS B3B68F89B6967047 virb7_rhird Outer membrane lipoprotein virB7 virB7 protein protein rhird Rhizobium radiobacter (Agrobacterium tumefaciens) Agrobacterium radiobacter Agrobacterium tumefaciens MKYCLLCLVVALSGCQTNDTIASCKGPIFPLNVGRWQPTPSDLQLRNSGGRYDGA 87909B60286A29B9 virb9_rhird Protein virB9 virB9 protein protein rhird Rhizobium radiobacter (Agrobacterium tumefaciens) Agrobacterium radiobacter Agrobacterium tumefaciens MTRKALFILACLFAAATGAEAEDTPMAGKLDPRMRYLAYNPDQVVRLSTAVGATLVVTFATNETVTSVAVSNSKDLAALPRGNYLFFKASQVLTPQPVIVLTASDSGMRRYVFSISSKTLSHLDKEQPDLYYSVQFAYPADDAAARRREAQQRAVVDRLHAEAQYQRKAEDLLDQPVTALGATDSNWHYVAQGDRSLLPLEVFDNGFTTVFHFPGNVRIPSIYTINPDGKEAVANYSVKGSDVEISSVSRGWRLRDGHTVLCIWNAAYDPVGQRPQTGTVRPDVKRVLKGAKG 1BA2E48058E7DD19 virb10-vird4 1 2 unspecified role unspecified role prey prey 3 unspecified role unspecified role bait bait region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain physical association physical association Fig. 2A virb10-virb7 1 5 unspecified role unspecified role prey prey 3 unspecified role unspecified role bait bait 4 unspecified role unspecified role prey prey association association Experiments were carried out under non-reducing conditions for co-precipitation of the VirB7–VirB9 dimer complex Fig. 2, 5B