mpidb The Microbial Protein Interaction Database Transcriptional modulator NusA interacts with translesion DNA polymerases in Escherichia coli. Transcriptional modulator NusA interacts with translesion DNA polymerases in Escherichia coli. J. Bacteriol. (0021-9193) 2009 imex curation Cohen S.E., Godoy V.G., Walker G.C. Only protein-protein interactions in vitro In vitro far western blotting far western blotting Affinity blotting Affinity blotting weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Cohen S.E., Godoy V.G., Walker G.C. Accepted 2013-JAN-31 by ORCHARD J. Bacteriol. (0021-9193) 2009 Only protein-protein interactions imex curation Transcriptional modulator NusA interacts with translesion DNA polymerases in Escherichia coli. Transcriptional modulator NusA interacts with translesion DNA polymerases in Escherichia coli. J. Bacteriol. (0021-9193) 2009 imex curation Cohen S.E., Godoy V.G., Walker G.C. Only protein-protein interactions ecoli Escherichia coli (strain K12) affinity chrom affinity chromatography technology Affinity purification Affinity purification affinity chrom edman degradation edman degradation Cohen S.E., Godoy V.G., Walker G.C. Accepted 2013-JAN-31 by ORCHARD J. Bacteriol. (0021-9193) 2009 Only protein-protein interactions imex curation Transcriptional modulator NusA interacts with translesion DNA polymerases in Escherichia coli. Transcriptional modulator NusA interacts with translesion DNA polymerases in Escherichia coli. J. Bacteriol. (0021-9193) 2009 imex curation Cohen S.E., Godoy V.G., Walker G.C. Only protein-protein interactions in vitro In vitro peptide array peptide array western blot western blot Immuno blot Immuno blot Cohen S.E., Godoy V.G., Walker G.C. Accepted 2013-JAN-31 by ORCHARD J. Bacteriol. (0021-9193) 2009 Only protein-protein interactions imex curation dpo4_ecoli DNA polymerase IV Translesion synthesis polymerase IV dinB dinP b0231 JW0221 protein protein ecoli Escherichia coli (strain K12) MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTANYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSLDEAYLDVTDSVHCHGSATLIAQEIRQTIFNELQLTASAGVAPVKFLAKIASDMNKPNGQFVITPAEVPAFLQTLPLAKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVMLLKRFGKFGRILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAKVKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKADLIATARKTWDERRGGRGVRLVGLHVTLLDPQMERQLVLGL 74DF44DCA18D405F nusa_ecoli Transcription termination/antitermination protein NusA N utilization substance protein A Transcription termination/antitermination L factor JW3138 b3169 nusA protein protein ecoli Escherichia coli (strain K12) MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEIDVRVQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTFDRITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLDLGNNAEAVILREDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGEEVIEIKAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSTELGGERIDIVLWDDNPAQFVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGWELNVMTVDDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKELLEIEGLDEPTVEALRERAKNALATIAQAQEESLGDNKPADDLLNLEGVDRDLAFKLAARGVCTLEDLAEQGIDDLADIEGLTDEKAGALIMAARNICWFGDEA DIP protein P0AFF6 original sequence version: 45 DIP protein P0AFF6 original sequence version: 60 This sequence has been withdrawn from Uniprot. 7D4DD019172FBAD0 ch60_ecoli Chaperonin GroEL groEL 60 kDa chaperonin Chaperonin-60 GroEL protein groL mopA JW4103 b4143 protein protein ecoli Escherichia coli (strain K12) MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSFGAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITEGLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGKLIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESPFILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAVKAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIIDGVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEKKARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAPLRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAASVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM CD3A0FB505F74AD1 CD3A0FB505F74AD1 dinb-grol 2 4 unspecified role unspecified role bait bait region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 5 unspecified role unspecified role prey prey 6 unspecified role unspecified role prey prey association association figure 1A q47155-nusa-1 1 4 unspecified role unspecified role bait bait region 32p radiolabel 32p radiolabel P32 32P 32P P32 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecolx Escherichia coli 5 unspecified role unspecified role prey prey region P0AFF6-232-495 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli physical association physical association figure 1C q47155-nusa-2 3 4 unspecified role unspecified role bait bait 5 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined chemical synthesis Chemical synthesis (Chemical synthesis) direct interaction direct interaction figure 2