UniProt UniProt http://www.uniprot.org RGS9-2 is a negative modulator of mu-opioid receptor function. RGS9-2 is a negative modulator of mu-opioid receptor function. J. Neurochem. (0022-3042) 2007 imex curation Psifogeorgou K., Papakosta P., Russo S.J., Neve R.L., Kardassis D., Gold S.J., Zachariou V. HA-MOR plasmid provided by von Zastrow who worked with mouse ( eg. see pmid 12764121 citing pmid 7797593) . Origin of RGS9_2 according pmid 10066255. Co-transfected Gbeta5 could not be mapped (not used as bait or prey protein, respectively). vzachar@med.upc.gr 20-Jun-2012: Contacted by JYOTI. Only protein-protein interactions rat-pc12 Rattus norvegicus PC12 cells pc12 Rat adrenal pheochromocytoma cells anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Psifogeorgou K., Papakosta P., Russo S.J., Neve R.L., Kardassis D., Gold S.J., Zachariou V. HA-MOR plasmid provided by von Zastrow who worked with mouse ( eg. see pmid 12764121 citing pmid 7797593) . Origin of RGS9_2 according pmid 10066255. Co-transfected Gbeta5 could not be mapped (not used as bait or prey protein, respectively). vzachar@med.upc.gr Accepted 2012-APR-30 by PPORRAS 20-Jun-2012: Contacted by JYOTI. J. Neurochem. (0022-3042) 2007 Only protein-protein interactions imex curation RGS9-2 is a negative modulator of mu-opioid receptor function. RGS9-2 is a negative modulator of mu-opioid receptor function. J. Neurochem. (0022-3042) 2007 imex curation Psifogeorgou K., Papakosta P., Russo S.J., Neve R.L., Kardassis D., Gold S.J., Zachariou V. HA-MOR plasmid provided by von Zastrow who worked with mouse ( eg. see pmid 12764121 citing pmid 7797593) . Origin of RGS9_2 according pmid 10066255. Co-transfected Gbeta5 could not be mapped (not used as bait or prey protein, respectively). vzachar@med.upc.gr 20-Jun-2012: Contacted by JYOTI. Only protein-protein interactions rat-pc12 Rattus norvegicus PC12 cells pc12 Rat adrenal pheochromocytoma cells anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Psifogeorgou K., Papakosta P., Russo S.J., Neve R.L., Kardassis D., Gold S.J., Zachariou V. HA-MOR plasmid provided by von Zastrow who worked with mouse ( eg. see pmid 12764121 citing pmid 7797593) . Origin of RGS9_2 according pmid 10066255. Co-transfected Gbeta5 could not be mapped (not used as bait or prey protein, respectively). vzachar@med.upc.gr Accepted 2012-APR-30 by PPORRAS 20-Jun-2012: Contacted by JYOTI. J. Neurochem. (0022-3042) 2007 Only protein-protein interactions imex curation RGS9-2 is a negative modulator of mu-opioid receptor function. RGS9-2 is a negative modulator of mu-opioid receptor function. J. Neurochem. (0022-3042) 2007 imex curation Psifogeorgou K., Papakosta P., Russo S.J., Neve R.L., Kardassis D., Gold S.J., Zachariou V. HA-MOR plasmid provided by von Zastrow who worked with mouse ( eg. see pmid 12764121 citing pmid 7797593) . Origin of RGS9_2 according pmid 10066255. Co-transfected Gbeta5 could not be mapped (not used as bait or prey protein, respectively). vzachar@med.upc.gr 20-Jun-2012: Contacted by JYOTI. Only protein-protein interactions rat-pc12 Rattus norvegicus PC12 cells pc12 Rat adrenal pheochromocytoma cells anti bait coip anti bait coimmunoprecipitation anti bait coip anti tag western anti tag western blot anti tag western Psifogeorgou K., Papakosta P., Russo S.J., Neve R.L., Kardassis D., Gold S.J., Zachariou V. HA-MOR plasmid provided by von Zastrow who worked with mouse ( eg. see pmid 12764121 citing pmid 7797593) . Origin of RGS9_2 according pmid 10066255. Co-transfected Gbeta5 could not be mapped (not used as bait or prey protein, respectively). vzachar@med.upc.gr Accepted 2012-APR-30 by PPORRAS 20-Jun-2012: Contacted by JYOTI. J. Neurochem. (0022-3042) 2007 Only protein-protein interactions imex curation RGS9-2 is a negative modulator of mu-opioid receptor function. RGS9-2 is a negative modulator of mu-opioid receptor function. J. Neurochem. (0022-3042) 2007 imex curation Psifogeorgou K., Papakosta P., Russo S.J., Neve R.L., Kardassis D., Gold S.J., Zachariou V. HA-MOR plasmid provided by von Zastrow who worked with mouse ( eg. see pmid 12764121 citing pmid 7797593) . Origin of RGS9_2 according pmid 10066255. Co-transfected Gbeta5 could not be mapped (not used as bait or prey protein, respectively). vzachar@med.upc.gr 20-Jun-2012: Contacted by JYOTI. Only protein-protein interactions rat-pc12 Rattus norvegicus PC12 cells pc12 Rat adrenal pheochromocytoma cells confocal microscopy confocal microscopy immunostaining immunostaining Psifogeorgou K., Papakosta P., Russo S.J., Neve R.L., Kardassis D., Gold S.J., Zachariou V. HA-MOR plasmid provided by von Zastrow who worked with mouse ( eg. see pmid 12764121 citing pmid 7797593) . Origin of RGS9_2 according pmid 10066255. Co-transfected Gbeta5 could not be mapped (not used as bait or prey protein, respectively). vzachar@med.upc.gr Accepted 2012-APR-30 by PPORRAS J. Neurochem. (0022-3042) 2007 Only protein-protein interactions imex curation o54828-1 Regulator of G-protein signaling 9 RGS9-2 Rgs9 protein protein mouse Mus musculus Mouse MTIRHQGQQYRPRMAFLQKIEALVKDMQNPETGVRMHNQRVLVTSVPHAMTGGDVLQWITQRLWISNLEAQNLGNFIVKYGYIYPLQDPKNLILKPDSSLYRFQTPYFWPTQQWPAEDTDYAIYLAKRNIKKKGILEEYEKENYDFLNKKINYKWDFVIMQAKEQYRTGKERNKADRYALDCQEKAYWLVHRSPPGMNNVLDYGLDRVTNPNEVKKQTVTAVRKEIMYYQQALMRSTVKSSVSLGGIVKYSEQFSSNDAIMSGCLPSNPWITDDTQFWDLNAKLVEIPTKMRVERWAFNFSELIRDPKGRQSFQYFLKKEFSGENLGFWEACEDLKYGDQSKVKEKAEEIYKLFLAPGARRWINIDGKTMDITVKGLRHPHRYVLDAAQTHIYMLMKKDSYARYLKSPIYKEMLAKAIEPQETTKRSSTLPFMRRHLRSSPSPVILRQLEEEEKAREAANTVDITQPGQHLAPSPHLAVYTGTCVPPSPSSPFSPSCRSPRKPFASPSRFIRRPSIAICPSPSRVALEGSSGLEPKGEASWSGANSGPSVTENREPSADHSRPQPRAPPKARAALSLGRFLRRGCLASPVFARLSPKCPSVSHGKVQPLGDMGQQLPRLKPKKVANFFQIKMEMPTDSGTCLMDSDDPRAGESGDQTTEKEVICPWESLAEGKAG 72F1652D7D59EB05 arrb2_rat Beta-arrestin-2 Arrb2 Arrestin beta-2 protein protein rat Rattus norvegicus (Rat) Rat MGEKPGTRVFKKSSPNCKLTVYLGKRDFVDHLDKVDPVDGVVLVDPDYLKDRKVFVTLTCAFRYGREDLDVLGLSFRKDLFIATYQAFPPMPNPPRPPTRLQDRLLKKLGQHAHPFFFTIPQNLPCSVTLQPGPEDTGKACGVDFEIRAFCAKSIEEKSHKRNSVRLIIRKVQFAPETPGPQPSAETTRHFLMSDRRSLHLEASLDKELYYHGEPLNVNVHVTNNSAKTVKKIRVSVRQYADICLFSTAQYKCPVAQLEQDDQVSPSSTFCKVYTITPLLSDNREKRGLALDGQLKHEDTNLASSTIVKEGANKEVLGILVSYRVKVKLVVSRGGDVSVELPFVLMHPKPHDHITLPRPQSAPREIDIPVDTNLIEFDTNYATDDDIVFEDFARLRLKGMKDDDCDDQFC DIP protein P29067 original sequence version: 115 DIP protein P29067 original sequence version: 112 DIP protein P29067 original sequence version: 100 0DFA6A897C2B86BA oprm_mouse Mu-type opioid receptor Oprm1 Mor Oprm protein protein mouse Mus musculus Mouse MDSSAGPGNISDCSDPLAPASCSPAPGSWLNLSHVDGNQSDPCGPNRTGLGGSHSLCPQTGSPSMVTAITIMALYSIVCVVGLFGNFLVMYVIVRYTKMKTATNIYIFNLALADALATSTLPFQSVNYLMGTWPFGNILCKIVISIDYYNMFTSIFTLCTMSVDRYIAVCHPVKALDFRTPRNAKIVNVCNWILSSAIGLPVMFMATTKYRQGSIDCTLTFSHPTWYWENLLKICVFIFAFIMPVLIITVCYGLMILRLKSVRMLSGSKEKDRNLRRITRMVLVVVAVFIVCWTPIHIYVIIKALITIPETTFQTVSWHFCIALGYTNSCLNPVLYAFLDENFKRCFREFCIPTSSTIEQQNSARIRQNTREHPSTANTVDRTNHQLENLEAETAPLP DIP protein P42866 original sequence version: 69 DIP protein P42866 original sequence version: 70 DFE1C758E2DA197E oprm1-rgs9 4 5 unspecified role unspecified role neutral component neutral component nucl transfection nucleic acid transfection nucl transfection region egfp tag enhanced green fluorescent protein tag enhanced green fluorescent protein EGFP enhanced green fluorescent protein EGFP egfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 7 unspecified role unspecified role neutral component neutral component nucl transfection nucleic acid transfection nucl transfection region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined colocalization colocalization 3 Morphine treatment rgs9-oprm1 3 5 unspecified role unspecified role bait bait nucl transfection nucleic acid transfection nucl transfection 7 unspecified role unspecified role prey prey nucl transfection nucleic acid transfection nucl transfection region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association 4a Morphine treatment Gbeta5 co-transfected rgs9-arrb2 1 5 unspecified role unspecified role bait bait nucl transfection nucleic acid transfection nucl transfection 6 unspecified role unspecified role prey prey physical association physical association 4b Morphine treatment Gbeta5 and HA-MOR co-transfected oprm1-arrb2 2 6 unspecified role unspecified role prey prey 7 unspecified role unspecified role bait bait nucl transfection nucleic acid transfection nucl transfection region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association 4c Overexpression (co-transfection) of RGS9-2 does not affect the interaction after morphine treatment. Morphine treatment.