UniProt UniProt http://www.uniprot.org ZNF366 is an estrogen receptor corepressor that acts through CtBP and histone deacetylases. ZNF366 is an estrogen receptor corepressor that acts through CtBP and histone deacetylases. Nucleic Acids Res. (0305-1048) 2006 mimix curation Lopez-Garcia J., Periyasamy M., Thomas R.S., Christian M., Leao M., Jat P., Kindle K.B., Heery D.M., Parker M.G., Buluwela L., Kamalati T., Ali S. Origin of ERalpha according sequence length. Origin of CTBP1 according ref 40 (pmid 8440238). Origin of RIP140 according sequence length. Exp. with radiolabeled HDAC proteins (fig. 6C) omitted as species origin cannot be established. simak.ali@imperial.ac.uk 14-Mar-2012: Contacted by JYOTI. human placental cDNA expression library (BD Biosciences, UK). chlae-cos_1 Chlorocebus aethiops COS-1 cells Grivet cos_1 CV-1 Simian cells (cercopithecus aethiops) transformed by SV40 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Lopez-Garcia J., Periyasamy M., Thomas R.S., Christian M., Leao M., Jat P., Kindle K.B., Heery D.M., Parker M.G., Buluwela L., Kamalati T., Ali S. Origin of ERalpha according sequence length. Origin of CTBP1 according ref 40 (pmid 8440238). Origin of RIP140 according sequence length. Exp. with radiolabeled HDAC proteins (fig. 6C) omitted as species origin cannot be established. simak.ali@imperial.ac.uk Accepted 2012-JAN-31 by MGT 14-Mar-2012: Contacted by JYOTI. human placental cDNA expression library (BD Biosciences, UK). Nucleic Acids Res. (0305-1048) 2006 mimix curation ZNF366 is an estrogen receptor corepressor that acts through CtBP and histone deacetylases. ZNF366 is an estrogen receptor corepressor that acts through CtBP and histone deacetylases. Nucleic Acids Res. (0305-1048) 2006 mimix curation Lopez-Garcia J., Periyasamy M., Thomas R.S., Christian M., Leao M., Jat P., Kindle K.B., Heery D.M., Parker M.G., Buluwela L., Kamalati T., Ali S. Origin of ERalpha according sequence length. Origin of CTBP1 according ref 40 (pmid 8440238). Origin of RIP140 according sequence length. Exp. with radiolabeled HDAC proteins (fig. 6C) omitted as species origin cannot be established. simak.ali@imperial.ac.uk 14-Mar-2012: Contacted by JYOTI. human placental cDNA expression library (BD Biosciences, UK). chlae-cos_1 Chlorocebus aethiops COS-1 cells Grivet cos_1 CV-1 Simian cells (cercopithecus aethiops) transformed by SV40 anti bait coip anti bait coimmunoprecipitation anti bait coip anti tag western anti tag western blot anti tag western Lopez-Garcia J., Periyasamy M., Thomas R.S., Christian M., Leao M., Jat P., Kindle K.B., Heery D.M., Parker M.G., Buluwela L., Kamalati T., Ali S. Origin of ERalpha according sequence length. Origin of CTBP1 according ref 40 (pmid 8440238). Origin of RIP140 according sequence length. Exp. with radiolabeled HDAC proteins (fig. 6C) omitted as species origin cannot be established. simak.ali@imperial.ac.uk Accepted 2012-JAN-31 by MGT 14-Mar-2012: Contacted by JYOTI. human placental cDNA expression library (BD Biosciences, UK). Nucleic Acids Res. (0305-1048) 2006 mimix curation ZNF366 is an estrogen receptor corepressor that acts through CtBP and histone deacetylases. ZNF366 is an estrogen receptor corepressor that acts through CtBP and histone deacetylases. Nucleic Acids Res. (0305-1048) 2006 mimix curation Lopez-Garcia J., Periyasamy M., Thomas R.S., Christian M., Leao M., Jat P., Kindle K.B., Heery D.M., Parker M.G., Buluwela L., Kamalati T., Ali S. Origin of ERalpha according sequence length. Origin of CTBP1 according ref 40 (pmid 8440238). Origin of RIP140 according sequence length. Exp. with radiolabeled HDAC proteins (fig. 6C) omitted as species origin cannot be established. simak.ali@imperial.ac.uk 14-Mar-2012: Contacted by JYOTI. human placental cDNA expression library (BD Biosciences, UK). yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Lopez-Garcia J., Periyasamy M., Thomas R.S., Christian M., Leao M., Jat P., Kindle K.B., Heery D.M., Parker M.G., Buluwela L., Kamalati T., Ali S. Origin of ERalpha according sequence length. Origin of CTBP1 according ref 40 (pmid 8440238). Origin of RIP140 according sequence length. Exp. with radiolabeled HDAC proteins (fig. 6C) omitted as species origin cannot be established. simak.ali@imperial.ac.uk Accepted 2012-JAN-31 by MGT 14-Mar-2012: Contacted by JYOTI. human placental cDNA expression library (BD Biosciences, UK). Nucleic Acids Res. (0305-1048) 2006 mimix curation ZNF366 is an estrogen receptor corepressor that acts through CtBP and histone deacetylases. ZNF366 is an estrogen receptor corepressor that acts through CtBP and histone deacetylases. Nucleic Acids Res. (0305-1048) 2006 mimix curation Lopez-Garcia J., Periyasamy M., Thomas R.S., Christian M., Leao M., Jat P., Kindle K.B., Heery D.M., Parker M.G., Buluwela L., Kamalati T., Ali S. Origin of ERalpha according sequence length. Origin of CTBP1 according ref 40 (pmid 8440238). Origin of RIP140 according sequence length. Exp. with radiolabeled HDAC proteins (fig. 6C) omitted as species origin cannot be established. simak.ali@imperial.ac.uk 14-Mar-2012: Contacted by JYOTI. human placental cDNA expression library (BD Biosciences, UK). in vitro In vitro pull down pull down weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Lopez-Garcia J., Periyasamy M., Thomas R.S., Christian M., Leao M., Jat P., Kindle K.B., Heery D.M., Parker M.G., Buluwela L., Kamalati T., Ali S. Origin of ERalpha according sequence length. Origin of CTBP1 according ref 40 (pmid 8440238). Origin of RIP140 according sequence length. Exp. with radiolabeled HDAC proteins (fig. 6C) omitted as species origin cannot be established. simak.ali@imperial.ac.uk Accepted 2012-JAN-31 by MGT 14-Mar-2012: Contacted by JYOTI. human placental cDNA expression library (BD Biosciences, UK). Nucleic Acids Res. (0305-1048) 2006 mimix curation ZNF366 is an estrogen receptor corepressor that acts through CtBP and histone deacetylases. ZNF366 is an estrogen receptor corepressor that acts through CtBP and histone deacetylases. Nucleic Acids Res. (0305-1048) 2006 mimix curation Lopez-Garcia J., Periyasamy M., Thomas R.S., Christian M., Leao M., Jat P., Kindle K.B., Heery D.M., Parker M.G., Buluwela L., Kamalati T., Ali S. Origin of ERalpha according sequence length. Origin of CTBP1 according ref 40 (pmid 8440238). Origin of RIP140 according sequence length. Exp. with radiolabeled HDAC proteins (fig. 6C) omitted as species origin cannot be established. simak.ali@imperial.ac.uk 14-Mar-2012: Contacted by JYOTI. human placental cDNA expression library (BD Biosciences, UK). chlae-cos_1 Chlorocebus aethiops COS-1 cells Grivet cos_1 CV-1 Simian cells (cercopithecus aethiops) transformed by SV40 anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Lopez-Garcia J., Periyasamy M., Thomas R.S., Christian M., Leao M., Jat P., Kindle K.B., Heery D.M., Parker M.G., Buluwela L., Kamalati T., Ali S. Origin of ERalpha according sequence length. Origin of CTBP1 according ref 40 (pmid 8440238). Origin of RIP140 according sequence length. Exp. with radiolabeled HDAC proteins (fig. 6C) omitted as species origin cannot be established. simak.ali@imperial.ac.uk Accepted 2012-JAN-31 by MGT 14-Mar-2012: Contacted by JYOTI. human placental cDNA expression library (BD Biosciences, UK). Nucleic Acids Res. (0305-1048) 2006 mimix curation ZNF366 is an estrogen receptor corepressor that acts through CtBP and histone deacetylases. ZNF366 is an estrogen receptor corepressor that acts through CtBP and histone deacetylases. Nucleic Acids Res. (0305-1048) 2006 mimix curation Lopez-Garcia J., Periyasamy M., Thomas R.S., Christian M., Leao M., Jat P., Kindle K.B., Heery D.M., Parker M.G., Buluwela L., Kamalati T., Ali S. Origin of ERalpha according sequence length. Origin of CTBP1 according ref 40 (pmid 8440238). Origin of RIP140 according sequence length. Exp. with radiolabeled HDAC proteins (fig. 6C) omitted as species origin cannot be established. simak.ali@imperial.ac.uk 14-Mar-2012: Contacted by JYOTI. human placental cDNA expression library (BD Biosciences, UK). yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Lopez-Garcia J., Periyasamy M., Thomas R.S., Christian M., Leao M., Jat P., Kindle K.B., Heery D.M., Parker M.G., Buluwela L., Kamalati T., Ali S. Origin of ERalpha according sequence length. Origin of CTBP1 according ref 40 (pmid 8440238). Origin of RIP140 according sequence length. Exp. with radiolabeled HDAC proteins (fig. 6C) omitted as species origin cannot be established. simak.ali@imperial.ac.uk Accepted 2012-JAN-31 by MGT human placental cDNA expression library (BD Biosciences, UK). Nucleic Acids Res. (0305-1048) 2006 mimix curation zn366_human Zinc finger protein 366 ZNF366 Dendritic cell-specific transcript protein protein protein human Homo sapiens Human MQKEMKMIKDEDVHFDLAVKKTPSFPHCLQPVASRGKAPQRHPFPEALRGPFSQFRYEPPPGDLDGFPGVFEGAGSRKRKSMPTKMPYNHPAEEVTLALHSEENKNHGLPNLPLLFPQPPRPKYDSQMIDLCNVGFQFYRSLEHFGGKPVKQEPIKPSAVWPQPTPTPFLPTPYPYYPKVHPGLMFPFFVPSSSPFPFSRHTFLPKQPPEPLLPRKAEPQESEETKQKVERVDVNVQIDDSYYVDVGGSQKRWQCPTCEKSYTSKYNLVTHILGHSGIKPHACTHCGKLFKQLSHLHTHMLTHQGTRPHKCQVCHKAFTQTSHLKRHMMQHSEVKPHNCRVCGRGFAYPSELKAHEAKHASGRENICVECGLDFPTLAQLKRHLTTHRGPIQYNCSECDKTFQYPSQLQNHMMKHKDIRPYICSECGMEFVQPHHLKQHSLTHKGVKEHKCGICGREFTLLANMKRHVLIHTNIRAYQCHLCYKSFVQKQTLKAHMIVHSDVKPFKCKLCGKEFNRMHNLMGHMHLHSDSKPFKCLYCPSKFTLKGNLTRHMKVKHGVMERGLHSQGLGRGRIALAQTAGVLRSLEQEEPFDLSQKRRAKVPVFQSDGESAQGSHCHEEEEEDNCYEVEPYSPGLAPQSQQLCTPEDLSTKSEHAPEVLEEACKEEKEDASKGEWEKRSKGDLGAEGGQERDCAGRDECLSLRAFQSTRRGPSFSDYLYFKHRDESLKELLERKMEKQAVLLGI D4E12259280727B1 nrip1_human Nuclear receptor-interacting protein 1 Nuclear factor RIP140 Receptor-interacting protein 140 NRIP1 protein protein human Homo sapiens Human MTHGEELGSDVHQDSIVLTYLEGLLMHQAAGGSGTAVDKKSAGHNEEDQNFNISGSAFPTCQSNGPVLNTHTYQGSGMLHLKKARLLQSSEDWNAAKRKRLSDSIMNLNVKKEALLAGMVDSVPKGKQDSTLLASLLQSFSSRLQTVALSQQIRQSLKEQGYALSHDSLKVEKDLRCYGVASSHLKTLLKKSKVKDQKPDTNLPDVTKNLIRDRFAESPHHVGQSGTKVMSEPLSCAARLQAVASMVEKRASPATSPKPSVACSQLALLLSSEAHLQQYSREHALKTQNANQAASERLAAMARLQENGQKDVGSYQLPKGMSSHLNGQARTSSSKLMASKSSATVFQNPMGIIPSSPKNAGYKNSLERNNIKQAANNSLLLHLLKSQTIPKPMNGHSHSERGSIFEESSTPTTIDEYSDNNPSFTDDSSGDESSYSNCVPIDLSCKHRTEKSESDQPVSLDNFTQSLLNTWDPKVPDVDIKEDQDTSKNSKLNSHQKVTLLQLLLGHKNEENVEKNTSPQGVHNDVSKFNTQNYARTSVIESPSTNRTTPVSTPPLLTSSKAGSPINLSQHSLVIKWNSPPYVCSTQSEKLTNTASNHSMDLTKSKDPPGEKPAQNEGAQNSATFSASKLLQNLAQCGMQSSMSVEEQRPSKQLLTGNTDKPIGMIDRLNSPLLSNKTNAVEENKAFSSQPTGPEPGLSGSEIENLLERRTVLQLLLGNPNKGKSEKKEKTPLRDESTQEHSERALSEQILMVKIKSEPCDDLQIPNTNVHLSHDAKSAPFLGMAPAVQRSAPALPVSEDFKSEPVSPQDFSFSKNGLLSRLLRQNQDSYLADDSDRSHRNNEMALLESKNLCMVPKKRKLYTEPLENPFKKMKNNIVDAANNHSAPEVLYGSLLNQEELKFSRNDLEFKYPAGHGSASESEHRSWARESKSFNVLKQLLLSENCVRDLSPHRSNSVADSKKKGHKNNVTNSKPEFSISSLNGLMYSSTQPSSCMDNRTFSYPGVVKTPVSPTFPEHLGCAGSRPESGLLNGCSMPSEKGPIKWVITDAEKNEYEKDSPRLTKTNPILYYMLQKGGNSVTSRETQDKDIWREASSAESVSQVTAKEELLPTAETKASFFNLRSPYNSHMGNNASRPHSANGEVYGLLGSVLTIKKESE 81FC424968E9A5F6 ctbp1_human C-terminal-binding protein 1 CTBP1 CTBP protein protein human Homo sapiens Human MGSSHLLNKGLPLGVRPPIMNGPLHPRPLVALLDGRDCTVEMPILKDVATVAFCDAQSTQEIHEKVLNEAVGALMYHTITLTREDLEKFKALRIIVRIGSGFDNIDIKSAGDLGIAVCNVPAASVEETADSTLCHILNLYRRATWLHQALREGTRVQSVEQIREVASGAARIRGETLGIIGLGRVGQAVALRAKAFGFNVLFYDPYLSDGVERALGLQRVSTLQDLLFHSDCVTLHCGLNEHNHHLINDFTVKQMRQGAFLVNTARGGLVDEKALAQALKEGRIRGAALDVHESEPFSFSQGPLKDAPNLICTPHAAWYSEQASIEMREEAAREIRRAITGRIPDSLKNCVNKDHLTAATHWASMDPAVVHPELNGAAYRYPPGVVGVAPTGIPAAVEGIVPSAMSLSHGLPPVAHPPHAPSPGQTVKPEADRDHASDQL DIP protein Q13363 original sequence version: 176 F071DD30B385603F esr1_human Estrogen receptor ESR1 ESR NR3A1 Estradiol receptor ER-alpha Nuclear receptor subfamily 3 group A member 1 protein protein human Homo sapiens Human MTMTLHTKASGMALLHQIQGNELEPLNRPQLKIPLERPLGEVYLDSSKPAVYNYPEGAAYEFNAAAAANAQVYGQTGLPYGPGSEAAAFGSNGLGGFPPLNSVSPSPLMLLHPPPQLSPFLQPHGQQVPYYLENEPSGYTVREAGPPAFYRPNSDNRRQGGRERLASTNDKGSMAMESAKETRYCAVCNDYASGYHYGVWSCEGCKAFFKRSIQGHNDYMCPATNQCTIDKNRRKSCQACRLRKCYEVGMMKGGIRKDRRGGRMLKHKRQRDDGEGRGEVGSAGDMRAANLWPSPLMIKRSKKNSLALSLTADQMVSALLDAEPPILYSEYDPTRPFSEASMMGLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEILMIGLVWRSMEHPGKLLFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEFVCLKSIILLNSGVYTFLSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRLAQLLLILSHIRHMSNKGMEHLYSMKCKNVVPLYDLLLEMLDAHRLHAPTSRGGASVEETDQSHLATAGSTSSHSLQKYYITGEAEGFPATV DIP protein P03372 original sequence version: 214 DIP protein P03372 original sequence version: 139 DIP protein P03372 original sequence version: 167 5455C57AB0CCCAA7 esr1-znf366 6 7 unspecified role unspecified role prey prey 10 unspecified role unspecified role bait bait physical association physical association no figure znf366-esr1 5 10 unspecified role unspecified role prey prey 7 unspecified role unspecified role bait bait physical association physical association 2A esr1-znf366-1 2 10 unspecified role unspecified role bait bait 7 unspecified role unspecified role prey prey physical association physical association 2B esr1-znf366-2 3 7 unspecified role unspecified role prey prey 10 unspecified role unspecified role bait bait physical association physical association 2C znf366-esr1-1 4 7 unspecified role unspecified role bait bait 10 unspecified role unspecified role prey prey direct interaction direct interaction 3C and D nrip1-znf366 4 8 unspecified role unspecified role bait bait 7 unspecified role unspecified role prey prey direct interaction direct interaction 5A nrip1-ctbp1 4 9 unspecified role unspecified role prey prey 8 unspecified role unspecified role bait bait direct interaction direct interaction 5A znf366-nrip1 4 7 unspecified role unspecified role bait bait 8 unspecified role unspecified role prey prey direct interaction direct interaction 5B znf366-ctbp1 4 7 unspecified role unspecified role bait bait 9 unspecified role unspecified role prey prey direct interaction direct interaction 5B ctbp1-znf366 4 7 unspecified role unspecified role prey prey 9 unspecified role unspecified role bait bait direct interaction direct interaction 5C znf366-ctbp1-1 1 7 unspecified role unspecified role bait bait 9 unspecified role unspecified role prey prey physical association physical association 5D ctbp1-znf366-1 2 9 unspecified role unspecified role bait bait 7 unspecified role unspecified role prey prey physical association physical association 5F znf366-ctbp1-2 5 7 unspecified role unspecified role bait bait 9 unspecified role unspecified role prey prey physical association physical association 5G esr1-znf366-1 4 10 unspecified role unspecified role bait bait 7 unspecified role unspecified role prey prey direct interaction direct interaction 5A