UniProt UniProt http://www.uniprot.org Frodo links Dishevelled to the p120-catenin/Kaiso pathway: distinct catenin subfamilies promote Wnt signals. Frodo links Dishevelled to the p120-catenin/Kaiso pathway: distinct catenin subfamilies promote Wnt signals. Dev. Cell (1534-5807) 2006 imex curation Park J.I., Ji H., Jun S., Gu D., Hikasa H., Li L., Sokol S.Y., McCrea P.D. pdmccrea@mdanderson.org 19-Sept-2012: Contacted by IntAct-Help. Only protein-protein interactions human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 imaging technique imaging technique microscopy immunostaining immunostaining Park J.I., Ji H., Jun S., Gu D., Hikasa H., Li L., Sokol S.Y., McCrea P.D. pdmccrea@mdanderson.org Accepted 2012-AUG-06 by ORCHARD 19-Sept-2012: Contacted by IntAct-Help. Dev. Cell (1534-5807) 2006 Only protein-protein interactions imex curation Frodo links Dishevelled to the p120-catenin/Kaiso pathway: distinct catenin subfamilies promote Wnt signals. Frodo links Dishevelled to the p120-catenin/Kaiso pathway: distinct catenin subfamilies promote Wnt signals. Dev. Cell (1534-5807) 2006 imex curation Park J.I., Ji H., Jun S., Gu D., Hikasa H., Li L., Sokol S.Y., McCrea P.D. pdmccrea@mdanderson.org 19-Sept-2012: Contacted by IntAct-Help. Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Park J.I., Ji H., Jun S., Gu D., Hikasa H., Li L., Sokol S.Y., McCrea P.D. pdmccrea@mdanderson.org Accepted 2012-AUG-06 by ORCHARD 19-Sept-2012: Contacted by IntAct-Help. Xenopus gastrula cDNA Dev. Cell (1534-5807) 2006 Only protein-protein interactions imex curation Frodo links Dishevelled to the p120-catenin/Kaiso pathway: distinct catenin subfamilies promote Wnt signals. Frodo links Dishevelled to the p120-catenin/Kaiso pathway: distinct catenin subfamilies promote Wnt signals. Dev. Cell (1534-5807) 2006 imex curation Park J.I., Ji H., Jun S., Gu D., Hikasa H., Li L., Sokol S.Y., McCrea P.D. pdmccrea@mdanderson.org 19-Sept-2012: Contacted by IntAct-Help. Only protein-protein interactions xenla-embryo Xenopus laevis embryo embryo Embryo Defined as the development stage prior to the initiation of tissue differentiation. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Park J.I., Ji H., Jun S., Gu D., Hikasa H., Li L., Sokol S.Y., McCrea P.D. pdmccrea@mdanderson.org Accepted 2012-AUG-06 by ORCHARD 19-Sept-2012: Contacted by IntAct-Help. Dev. Cell (1534-5807) 2006 Only protein-protein interactions imex curation Frodo links Dishevelled to the p120-catenin/Kaiso pathway: distinct catenin subfamilies promote Wnt signals. Frodo links Dishevelled to the p120-catenin/Kaiso pathway: distinct catenin subfamilies promote Wnt signals. Dev. Cell (1534-5807) 2006 imex curation Park J.I., Ji H., Jun S., Gu D., Hikasa H., Li L., Sokol S.Y., McCrea P.D. pdmccrea@mdanderson.org 19-Sept-2012: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Park J.I., Ji H., Jun S., Gu D., Hikasa H., Li L., Sokol S.Y., McCrea P.D. pdmccrea@mdanderson.org Accepted 2012-AUG-06 by ORCHARD 19-Sept-2012: Contacted by IntAct-Help. Dev. Cell (1534-5807) 2006 Only protein-protein interactions imex curation Frodo links Dishevelled to the p120-catenin/Kaiso pathway: distinct catenin subfamilies promote Wnt signals. Frodo links Dishevelled to the p120-catenin/Kaiso pathway: distinct catenin subfamilies promote Wnt signals. Dev. Cell (1534-5807) 2006 imex curation Park J.I., Ji H., Jun S., Gu D., Hikasa H., Li L., Sokol S.Y., McCrea P.D. pdmccrea@mdanderson.org 19-Sept-2012: Contacted by IntAct-Help. Only protein-protein interactions xenla-embryo Xenopus laevis embryo embryo Embryo Defined as the development stage prior to the initiation of tissue differentiation. anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Park J.I., Ji H., Jun S., Gu D., Hikasa H., Li L., Sokol S.Y., McCrea P.D. pdmccrea@mdanderson.org Accepted 2012-AUG-06 by ORCHARD 19-Sept-2012: Contacted by IntAct-Help. Dev. Cell (1534-5807) 2006 Only protein-protein interactions imex curation Frodo links Dishevelled to the p120-catenin/Kaiso pathway: distinct catenin subfamilies promote Wnt signals. Frodo links Dishevelled to the p120-catenin/Kaiso pathway: distinct catenin subfamilies promote Wnt signals. Dev. Cell (1534-5807) 2006 imex curation Park J.I., Ji H., Jun S., Gu D., Hikasa H., Li L., Sokol S.Y., McCrea P.D. pdmccrea@mdanderson.org 19-Sept-2012: Contacted by IntAct-Help. Only protein-protein interactions xenla-embryo Xenopus laevis embryo embryo Embryo Defined as the development stage prior to the initiation of tissue differentiation. anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Park J.I., Ji H., Jun S., Gu D., Hikasa H., Li L., Sokol S.Y., McCrea P.D. pdmccrea@mdanderson.org Accepted 2012-AUG-06 by ORCHARD 19-Sept-2012: Contacted by IntAct-Help. Dev. Cell (1534-5807) 2006 Only protein-protein interactions imex curation dvl2_xenla Segment polarity protein dishevelled homolog DVL-2 dvl2 dsh DSH homolog 2 Xdsh protein protein xenla Xenopus laevis (African clawed frog) African clawed frog MAETKVIYHLDEEETPYLVKVPVPATDIRLRDFKAALGRGHAKYFFKAMDQDFGVVKEEISDDNAKLPCFNDRVVSWLASSEGSQPDSAPPAPATEVRPEPPPPVPPPIPPPPAERTSGIGDSRPPSFHPNVSGSTEQLDQDNESVISMRRDRVRRRESSEQAGVGRGVNGRTERHLSGYESSSTLLTSEIETSICDSEEDDTMSRFSSSTEQSSASRLLKRHRRRRKQRPPRLERTSSFSSVTDSTMSLNIITVTLNMEKYNFLGISIVGQSNERGDGGIYIGSIMKGGAVAADGRIEPGDMLLQVNDINFENMSNDDAVRVLRDIVHKPGPIVLTVAKCWDPSPQGYFTLPRNEPIHPIDPAAWVSHSAALSGSFPVYPGSASMSSMTSSTSVTETELSHALPPVSLFSLSVHTDLASVVKVMASPESGLEVRDRMWLKITIPNAFLGSDVVDWLYHHVEGFQDRREARKFASNLLKAGFIRHTVNKITFSEQCYYIFGDLTGCENYMTNLSLNDNDGSSGASDQDTLAPLPLPGASPWPLLPTFSYQYQAPHPYSTQPPAYHELSSYSYGMGSAGSQHSEGSRSSGSNRSDGGRGMQKDDRSGVAGVGGGDSKSGSGSESEYSTRSSIRRVGGGEAGPPSERSTSSRLPPHHPPSVHSYAAPGVPLSYNPMMLMMMPPPPLPPPGVCPPNSSVPPGAPPLVRDLASVPPELTATRQSFHMAMGNPSEFFVDVM AF6C9A1662DD7CEB ctnd1_xenla Catenin delta-1 ctnnd1 Xp120(ctn) p120 catenin protein protein xenla Xenopus laevis (African clawed frog) African clawed frog MDEPESESPASILASVRAQEAQFELLSRALEEERRHVTAQLDRVWVTPQEPPLANGSLTRRQQDGLPFLYTPTRMEAHSVHADERYVIDDSSYKSCTLTDESRCSEIPIQTVVGYSQTLDRPYREAAGSGGAYTTVPRNYHFRGPGVDSTMPLISQSPHPGYSSLSRPNQRYRSVDPFRGPGYGPQPQVRGGGLGGSQSDLLSGRIYGSEDAYGLEDDRRSLGGFIDGPDYATTGRRGANGGDPRRRLRSYEDPLSDHFGSIPTSGSLSSLGPAPLTLAPNSGPWRHPELPEVLAMLSYTLDAVRLNAAAYLQHLSYRNEDVKREVCRLRGIPPLISLLEDPRAPIRLAACGALKNLSYGPARENKMAVKNCDGVPALARLLRRRGEGIEGRELAECVTGTLWNLSSLDSVKMELVDQALYTLTQEILVPHSGWQQDGGMQDRVEGKPRHVEWEPALVNTTGCLRNISSERSEARRKMRECEGLVDSVVHILRSEVSHGLVDSKLLENVVCLLRNISYHVHREIPHAEKYMESPQNASAETQNPSCFGVRRGKGKKASEEAVDTVDFPKQTMPAQGYDLLFQPEIVRLYISLVKSSHTPAVLEASAGAIQNLCAGNWVYGRCIRAAVRQEKGLSSLADHLTHESERVVRAICGALRNLCGDNRNRELIGKHALNSLVARLSSSSAQSSALSEDTNVCVINTIHEVISGNLEAAKRLRESQGIERLVLINKGGGRSEREIRAAGFCLQTIWGYKELRRPLEKDGWKKSDFQVSTAATSSVQGYDDSTLPLIDRNLKNEKKSVNADIPLNDFIADQFTHNGDSSNRLPTRSDELSELDPLKECSVSAGGSLNLGDAEDQRV 7FE6744BCECF8C41 dct1a_xenla Dapper 1-A Dapper1b dact1-a Functional regulator of dsh in ontogenesis protein protein xenla Xenopus laevis (African clawed frog) African clawed frog MKPIPSPEPPGQLRLTPRRKDKGEAEWERHRTRERLEATLAGLAELDCLRHRQQLLVQNVLSPGTHGQDAAPTGDSPRSDEQKLLEENISLLKKQLNCLRKRDAGLLSQLHELDKQINDLKIDSEKTEETDSRPSSGFYELSDGTSGSLSNSSNSVFSECLSSCHSSTCFCNPLETSLNLTDGQAKSAEDFLEWLDYRESQHETGTVRCSFSAPHSNSVEVTADVHPKYQCDLVSKNGNDVYRYPSPLHAVAVQSPMFLISVMGNIKAEEPEEEIGPNDNDDCIVPELDHLKDEDSFLHQSSLCSLPLSSGKKMDGYILSIIHKKAHPVRTNKPRTSVNADPGKGILRHGSICVKQTVGVSQSNAVNLKNSKQTCLHSAGMISVDNSTYPSLKPCSKESLSEQLESKRMPSISTYPSCNVNELQSQNNSRNTVKSVCQGLARGSVAMTSNVQKENVTPNAPANLPNASSSVCNGSPRESTQNSALLPQEIKVVPPVKRVSLKNTLLSYHESSSFEERPPLDFKSEGSSSQSLDEGMLVNAHYIPAQQHGVKLHKNTKNVKIVKSSTLKHRADVQYVLENGSQTLKEKSKVVGKKCRFPEDLDTNKKVKKSTPRVKKIVHPHFEPAAVGRNPVAVRSGIKSHGHPKDVVLAKPKHKRSDYRRWKSSAEISYEEALRRARRRVQREMMGVCAQVPFSHASPYAYIASDSEYSAECESLFHSTVVDTSEDEQSNYTTNCFGDSESSLSEVEFVGESTTSSDTDESGGLIWSQFVHTLPMQATATAELQTTAKAFIKIKASHNLKKKILRFRSGSLKLMTTV FBBF0F3B193416A6 kaiso_xenla Transcriptional regulator Kaiso zbtb33 kaiso Zinc finger and BTB domain-containing protein 33 protein protein xenla Xenopus laevis (African clawed frog) African clawed frog METKKLITATDTQYSGILLNALNDQRIQGLYCDVTVIVEDRKFRAHRNILSACSTYFHQLFSVAGQVVELNFVKADIFAEILNYIYSSKIVRVRCDMLEELIKSGKLLGVPFIAELGIPLSQVKSISGAGGKDGGTDAPSNPDHKAPEPQKSSDSPLPCTVKIKADVKTEMPVITESFSLSSDDYKDKKASGSQDHNSEKEDDDDDVIFCSEIVSSKQAPAERKEAAQTQIPPDNEQVPEVKKVTPSSQVQLTQNSLPTNQQSSKNTSSTTQKFTPPVNANISKNPTPAANGFLSPTAQKQGTPNAVQNQHSQNITSGNALPQQKPVVNFSSIKPQQISAIKPKTEVIIHGNGLSPPSSSVIPLGQQPVTPKHISFDGVQKKQVVTFTQGSPSKPGEFKIKIADVVSGSSLDSFKDSEPRRIIDGKKIITLDTASEIEGLSTGCKVYANIGEDTYDIVIPIKEDPEEGEAKLDLDGLPNRKRMKLKHDDHYELIVDGRVYYICIVCKRSYVCLTSLRRHFNVHSWEKKYPCRYCERVFPLAEYRTKHEIHHTGERRYQCLTCGSSFINYQVMASHIRSVHSLDPSGDSKLYRLNPCKTLQIRQYAYVNNSTNGTVINDGAINVPVITDGGINVPVINDGGIVYDIDPDEPQQPASEGNHANSATKPVNWDNIFIQQSNQNMFKLNTSEGGTEFEFVIPESY 7556FC6F1049855F dact1_a-ctnnd1 3 8 unspecified role unspecified role prey prey 9 unspecified role unspecified role bait bait physical association physical association 1A dact1_a-ctnnd1-1 6 9 unspecified role unspecified role bait bait nucl microinjection nucleic acid microinjection nucl microinjection region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 8 unspecified role unspecified role prey prey physical association physical association 1B dact1_a-ctnnd1-2 5 9 unspecified role unspecified role bait bait nucl microinjection nucleic acid microinjection nucl microinjection region required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain n-terminal myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain 8 unspecified role unspecified role prey prey nucl microinjection nucleic acid microinjection nucl microinjection region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association 1C and E dact1_a-ctnnd1-3 2 8 unspecified role unspecified role prey prey c-terminal sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined c-term range C-terminal range c-term range 9 unspecified role unspecified role bait bait physical association physical association no figure zbtb33-ctnnd1-3 5 10 unspecified role unspecified role bait bait nucl microinjection nucleic acid microinjection nucl microinjection region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 8 unspecified role unspecified role prey prey nucl microinjection nucleic acid microinjection nucl microinjection region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association 5D dact1-a/frodo (UniProt Ac Q8JJ48) ctnnd1-dvl2 5 7 unspecified role unspecified role prey prey nucl microinjection nucleic acid microinjection nucl microinjection region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 8 unspecified role unspecified role bait bait nucl microinjection nucleic acid microinjection nucl microinjection region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association 6H dvl2-ctnnd1 5 7 unspecified role unspecified role bait bait nucl microinjection nucleic acid microinjection nucl microinjection region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 8 unspecified role unspecified role prey prey nucl microinjection nucleic acid microinjection nucl microinjection region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association 6H ctnnd1-zbtb33-5 4 10 unspecified role unspecified role unspecified role unspecified role in vitro translated in vitro translated protein in vitro translated region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined rabit-reticulocyte Oryctolagus cuniculus reticulocyte reticulocyte Reticulocytes 8 unspecified role unspecified role bait bait in vitro translated in vitro translated protein in vitro translated region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined rabit-reticulocyte Oryctolagus cuniculus reticulocyte reticulocyte Reticulocytes physical association physical association 6I ctnnd1-dact1_a 4 9 unspecified role unspecified role prey prey in vitro translated in vitro translated protein in vitro translated region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined rabit-reticulocyte Oryctolagus cuniculus reticulocyte reticulocyte Reticulocytes 8 unspecified role unspecified role bait bait in vitro translated in vitro translated protein in vitro translated region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined rabit-reticulocyte Oryctolagus cuniculus reticulocyte reticulocyte Reticulocytes physical association physical association 6I ctnnd1-dact1_a-1 1 9 unspecified role unspecified role neutral component neutral component nucl transfection nucleic acid transfection nucl transfection region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 8 unspecified role unspecified role neutral component neutral component nucl transfection nucleic acid transfection nucl transfection colocalization colocalization S1