UniProt UniProt http://www.uniprot.org The brefeldin A-inhibited GDP/GTP exchange factor 2, a protein involved in vesicular trafficking, interacts with the beta subunits of the GABA receptors. The brefeldin A-inhibited GDP/GTP exchange factor 2, a protein involved in vesicular trafficking, interacts with the beta subunits of the GABA receptors. J. Neurochem. (0022-3042) 2004 imex curation Charych E.I., Yu W., Miralles C.P., Serwanski D.R., Li X., Rubio M., De Blas A.L. Origin of GABA(A)R constructs according pmid 2548852. Binding sites for Gabrb1 and Gabrb2 (IL = intracellular/cytoplasmic loops) according UniProt). Colocalization studies using unspecific anti-beta2/3 GABA(A)R antibodies have been omitted. deblas@oracle.pnb.uconn.edu 19-Sept-2012: Contacted by IntAct-Help. Only protein-protein interactions human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. imaging technique imaging technique microscopy immunostaining immunostaining Charych E.I., Yu W., Miralles C.P., Serwanski D.R., Li X., Rubio M., De Blas A.L. Origin of GABA(A)R constructs according pmid 2548852. Binding sites for Gabrb1 and Gabrb2 (IL = intracellular/cytoplasmic loops) according UniProt). Colocalization studies using unspecific anti-beta2/3 GABA(A)R antibodies have been omitted. deblas@oracle.pnb.uconn.edu Accepted 2012-AUG-07 by JYOTI 19-Sept-2012: Contacted by IntAct-Help. J. Neurochem. (0022-3042) 2004 Only protein-protein interactions imex curation The brefeldin A-inhibited GDP/GTP exchange factor 2, a protein involved in vesicular trafficking, interacts with the beta subunits of the GABA receptors. The brefeldin A-inhibited GDP/GTP exchange factor 2, a protein involved in vesicular trafficking, interacts with the beta subunits of the GABA receptors. J. Neurochem. (0022-3042) 2004 imex curation Charych E.I., Yu W., Miralles C.P., Serwanski D.R., Li X., Rubio M., De Blas A.L. Origin of GABA(A)R constructs according pmid 2548852. Binding sites for Gabrb1 and Gabrb2 (IL = intracellular/cytoplasmic loops) according UniProt). Colocalization studies using unspecific anti-beta2/3 GABA(A)R antibodies have been omitted. deblas@oracle.pnb.uconn.edu 19-Sept-2012: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro far western blotting far western blotting Affinity blotting Affinity blotting western blot western blot Immuno blot Immuno blot Charych E.I., Yu W., Miralles C.P., Serwanski D.R., Li X., Rubio M., De Blas A.L. Origin of GABA(A)R constructs according pmid 2548852. Binding sites for Gabrb1 and Gabrb2 (IL = intracellular/cytoplasmic loops) according UniProt). Colocalization studies using unspecific anti-beta2/3 GABA(A)R antibodies have been omitted. deblas@oracle.pnb.uconn.edu Accepted 2012-AUG-07 by JYOTI 19-Sept-2012: Contacted by IntAct-Help. J. Neurochem. (0022-3042) 2004 Only protein-protein interactions imex curation The brefeldin A-inhibited GDP/GTP exchange factor 2, a protein involved in vesicular trafficking, interacts with the beta subunits of the GABA receptors. The brefeldin A-inhibited GDP/GTP exchange factor 2, a protein involved in vesicular trafficking, interacts with the beta subunits of the GABA receptors. J. Neurochem. (0022-3042) 2004 imex curation Charych E.I., Yu W., Miralles C.P., Serwanski D.R., Li X., Rubio M., De Blas A.L. Origin of GABA(A)R constructs according pmid 2548852. Binding sites for Gabrb1 and Gabrb2 (IL = intracellular/cytoplasmic loops) according UniProt). Colocalization studies using unspecific anti-beta2/3 GABA(A)R antibodies have been omitted. deblas@oracle.pnb.uconn.edu 19-Sept-2012: Contacted by IntAct-Help. Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Charych E.I., Yu W., Miralles C.P., Serwanski D.R., Li X., Rubio M., De Blas A.L. Origin of GABA(A)R constructs according pmid 2548852. Binding sites for Gabrb1 and Gabrb2 (IL = intracellular/cytoplasmic loops) according UniProt). Colocalization studies using unspecific anti-beta2/3 GABA(A)R antibodies have been omitted. deblas@oracle.pnb.uconn.edu Accepted 2012-AUG-07 by JYOTI 19-Sept-2012: Contacted by IntAct-Help. J. Neurochem. (0022-3042) 2004 Only protein-protein interactions imex curation The brefeldin A-inhibited GDP/GTP exchange factor 2, a protein involved in vesicular trafficking, interacts with the beta subunits of the GABA receptors. The brefeldin A-inhibited GDP/GTP exchange factor 2, a protein involved in vesicular trafficking, interacts with the beta subunits of the GABA receptors. J. Neurochem. (0022-3042) 2004 imex curation Charych E.I., Yu W., Miralles C.P., Serwanski D.R., Li X., Rubio M., De Blas A.L. Origin of GABA(A)R constructs according pmid 2548852. Binding sites for Gabrb1 and Gabrb2 (IL = intracellular/cytoplasmic loops) according UniProt). Colocalization studies using unspecific anti-beta2/3 GABA(A)R antibodies have been omitted. deblas@oracle.pnb.uconn.edu 19-Sept-2012: Contacted by IntAct-Help. Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Charych E.I., Yu W., Miralles C.P., Serwanski D.R., Li X., Rubio M., De Blas A.L. Origin of GABA(A)R constructs according pmid 2548852. Binding sites for Gabrb1 and Gabrb2 (IL = intracellular/cytoplasmic loops) according UniProt). Colocalization studies using unspecific anti-beta2/3 GABA(A)R antibodies have been omitted. deblas@oracle.pnb.uconn.edu Accepted 2012-AUG-07 by JYOTI 19-Sept-2012: Contacted by IntAct-Help. J. Neurochem. (0022-3042) 2004 Only protein-protein interactions imex curation The brefeldin A-inhibited GDP/GTP exchange factor 2, a protein involved in vesicular trafficking, interacts with the beta subunits of the GABA receptors. The brefeldin A-inhibited GDP/GTP exchange factor 2, a protein involved in vesicular trafficking, interacts with the beta subunits of the GABA receptors. J. Neurochem. (0022-3042) 2004 imex curation Charych E.I., Yu W., Miralles C.P., Serwanski D.R., Li X., Rubio M., De Blas A.L. Origin of GABA(A)R constructs according pmid 2548852. Binding sites for Gabrb1 and Gabrb2 (IL = intracellular/cytoplasmic loops) according UniProt). Colocalization studies using unspecific anti-beta2/3 GABA(A)R antibodies have been omitted. deblas@oracle.pnb.uconn.edu 19-Sept-2012: Contacted by IntAct-Help. Only protein-protein interactions rat-brain-hippocampus Hippocampus of rat brain rat-brain-hippocampus Hippocampus of rat brain anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Charych E.I., Yu W., Miralles C.P., Serwanski D.R., Li X., Rubio M., De Blas A.L. Origin of GABA(A)R constructs according pmid 2548852. Binding sites for Gabrb1 and Gabrb2 (IL = intracellular/cytoplasmic loops) according UniProt). Colocalization studies using unspecific anti-beta2/3 GABA(A)R antibodies have been omitted. deblas@oracle.pnb.uconn.edu Accepted 2012-AUG-07 by JYOTI 19-Sept-2012: Contacted by IntAct-Help. J. Neurochem. (0022-3042) 2004 Only protein-protein interactions imex curation gbrb3_rat Gamma-aminobutyric acid receptor subunit beta-3 Gabrb3 Gabrb-3 GABA(A) receptor subunit beta-3 protein protein rat Rattus norvegicus (Rat) Rat MWGFAGGRLFGIFSAPVLVAVVCCAQSVNDPGNMSFVKETVDKLLKGYDIRLRPDFGGPPVCVGMNIDIASIDMVSEVNMDYTLTMYFQQYWRDKRLAYSGIPLNLTLDNRVADQLWVPDTYFLNDKKSFVHGVTVKNRMIRLHPDGTVLYGLRITTTAACMMDLRRYPLDEQNCTLEIESYGYTTDDIEFYWRGGDKAVTGVERIELPQFSIVEHRLVSRNVVFATGAYPRLSLSFRLKRNIGYFILQTYMPSILITILSWVSFWINYDASAARVALGITTVLTMTTINTHLRETLPKIPYVKAIDMYLMGCFVFVFLALLEYAFVNYIFFGRGPQRQKKLAEKTAKAKNDRSKSEINRVDAHGNILLAPMDVHNEMNEVAGSVGDTRNSAISFDNSGIQYRKQSMPKEGHGRYMGDRSIPHKKTHLRRRSSQLKIKIPDLTDVNAIDRWSRIVFPFTFSLFNLVYWLYYVN DIP protein P63079 original sequence version: 84 6A5ABD8A9143FE45 big2_rat Brefeldin A-inhibited guanine nucleotide-exchange protein 2 ADP-ribosylation factor guanine nucleotide-exchange factor 2 Arfgep2 Arfgef2 Big2 protein protein rat Rattus norvegicus (Rat) Rat MQESQTKSMFVSRALEKILADKEVKRPQHSQLRRACQVALDEIKAELEKQRLGAAAPPKANFIEADKYFLPFELACQSKSPRVVSTSLDCLQKLIAYGHITGNAPDSGAPGKRLIDRIVETVCNCFQGPQTDEGVQLQIIKALLTAVTSPHIEIHEGTILQTVRTCYNIYLASKNLINQTTAKATLTQMLNVIFTRMENQVLQEARELEKPIQSKPQSPVIQATAGSPKFSRLKQSQAQSKPTTPEKTELPNGDHARSSLGKVNSENGEAHRERGSSISGRAEPSGGSDNGAQEVVKDILEDVVTSAVKEAAEKQGLPEPDQAPGVPECQECTVPPAVDENSQTNGIADDRQSLSSADNLEPDAQGHPVAARFSHILQKDAFLVFRSLCKLSMKPLGEGPPDPKSHELRSKVVSLQLLLSVLQNAGPVFRSHEMFVTAIKQYLCVALSKNGVSSVPDVFELSLAIFLTLLSNFKMHLKMQIEVFFKEIFLNILETSTSSFEHRWMVIQTLTRICADAQCVVDIYVNYDCDLNAANIFERLVNDLSKIAQGRSGHELGMTPLQELSLRKKGLECLVSILKCMVEWSKDLYVNPNHQATLGQERLPDQEMGDGKGLDMARRCSVTSVESTVSSGTQTAIPDDPEQFEVIKQQKEIIEHGIELFNKKPKRGIQFLQEQGMLGAAVEDIAQFLHQEERLDSTQVGEFLGDSTRFNKEVMYAYVDQLDFCEKEFVSALRTFLEGFRLPGEAQKIDRLMEKFAARYIECNQGQTLFASADTAYVLAYSIIMLTTDLHSPQVKNKMTKEQYIKMNRGINDSKDLPEEYLSSIYEEIEGKKIAMKETKEHTMATKSTKQNVASEKQRRLLYNVEMEQMAKTAKALMEAVSHAKAPFTSATHLDHVRPMFKLVWTPLLAAYSIGLQNCDDTEVASLCLEGIRCAVRIACIFGMQLERDAYVQALARFSLLTASSSITEMKQKNIDTIKTLITVAHTDGNYLGNSWHEILKCISQLELAQLIGTGVKTRYLSGSGREREGSLKGHSLAGEEFMGLGLGNLVSGGVDKRQMASFQESVGETSSQSVVVAVDRIFTGSTRLDGNAIVDFVRWLCAVSMDELASPHHPRMFSLQKIVEISYYNMNRIRLQWSRIWHVIGDHFNKVGCNPNEDVAIFAVDSLRQLSMKFLEKGELANFRFQKDFLRPFEHIMKKNRSPTIRDMVIRCIAQMVSSQAANIRSGWKNIFAVFHQAASDHDGNIVELAFQTTGHIVSTIFQHHFPAAIDSFQDAVKCLSEFACNAAFPDTSMEAIRLIRFCGKYVSERPRVLQEYTSDDMNVAPGDRVWVRGWFPILFELSCIINRCKLDVRTRGLTVMFEIMKSYGHTFAKHWWQDLFRIVFRIFDNMKLPEQQSEKSEWMTTTCNHALYAICDVFTQFYEALHEVLLSDVFAQLQWCVKQDNEQLARSGTNCLENLVISNGEKFSPAVWDETCNCMLDIFRTTIPHVLLTWRPAGMEEEVSDRHLDVDLDRQSLSSIDRNASERGQSQLSNPTDDSWKGAPYANQKLLASLLIKCVVQLELIQTIDNIVFYPATSKKEDAEHMVAAQQDTLDADIHIETENQGMYKFMSSQHLFKLLDCLQESHSFSKAFNSNYEQRTVLWRAGFKGKSKPNLLKQETSSLACCLRILFRMYVDENRRDSWGEIQQRLLTVCSEALAYFITVNSESHREAWTSLLLLLLTKTLKINDEKFKAHASVYYPYLCEMMQFDLIPELRAVLRKFFLRIGLVYKIWVPEEPSQVPAASTAW 397CB889C2ABF569 gbrb1_rat Gamma-aminobutyric acid receptor subunit beta-1 Gabrb1 Gabrb-1 GABA(A) receptor subunit beta-1 protein protein rat Rattus norvegicus (Rat) Rat MWTVQNRESLGLLSFPVMVAMVCCAHSSNEPSNMSYVKETVDRLLKGYDIRLRPDFGGPPVDVGMRIDVASIDMVSEVNMDYTLTMYFQQSWKDKRLSYSGIPLNLTLDNRVADQLWVPDTYFLNDKKSFVHGVTVKNRMIRLHPDGTVLYGLRITTTAACMMDLRRYPLDEQNCTLEIESYGYTTDDIEFYWNGGEGAVTGVNKIELPQFSIVDYKMVSKKVEFTTGAYPRLSLSFRLKRNIGYFILQTYMPSTLITILSWVSFWINYDASAARVALGITTVLTMTTISTHLRETLPKIPYVKAIDIYLMGCFVFVFLALLEYAFVNYIFFGKGPQKKGASKQDQSANEKNKLEMNKVQVDAHGNILLSTLEIRNETSGSEVLTGVSDPKATMYSYDSASIQYRKPLSSREGFGRGLDRHGVPGKGRIRRRASQLKVKIPDLTDVNSIDKWSRMFFPITFSLFNVVYWLYYVH 986277FDBD66377A gbrb2_rat Gamma-aminobutyric acid receptor subunit beta-2 Gabrb2 Gabrb-2 GABA(A) receptor subunit beta-2 protein protein rat Rattus norvegicus (Rat) Rat MWRVRKRGYFGIWSFPLIIAAVCAQSVNDPSNMSLVKETVDRLLKGYDIRLRPDFGGPPVAVGMNIDIASIDMVSEVNMDYTLTMYFQQAWRDKRLSYNVIPLNLTLDNRVADQLWVPDTYFLNDKKSFVHGVTVKNRMIRLHPDGTVLYGLRITTTAACMMDLRRYPLDEQNCTLEIESYGYTTDDIEFYWRGDDNAVTGVTKIELPQFSIVDYKLITKKVVFSTGSYPRLSLSFKLKRNIGYFILQTYMPSILITILSWVSFWINYDASAARVALGITTVLTMTTINTHLRETLPKIPYVKAIDMYLMGCFVFVFMALLEYALVNYIFFGRGPQRQKKAAEKAANANNEKMRLDVNKMDPHENILLSTLEIKNEMATSEAVMGLGDPRSTMLAYDASSIQYRKAGLPRHSFGRNALERHVAQKKSRLRRRASQLKITIPDLTDVNAIDRWSRIFFPVVFSFFNIVYWLYYVN 8A815982EB70933E gbra1_rat Gamma-aminobutyric acid receptor subunit alpha-1 Gabra1 Gabra-1 GABA(A) receptor subunit alpha-1 protein protein rat Rattus norvegicus (Rat) Rat MKKSRGLSDYLWAWTLILSTLSGRSYGQPSQDELKDNTTVFTRILDRLLDGYDNRLRPGLGERVTEVKTDIFVTSFGPVSDHDMEYTIDVFFRQSWKDERLKFKGPMTVLRLNNLMASKIWTPDTFFHNGKKSVAHNMTMPNKLLRITEDGTLLYTMRLTVRAECPMHLEDFPMDAHACPLKFGSYAYTRAEVVYEWTREPARSVVVAEDGSRLNQYDLLGQTVDSGIVQSSTGEYVVMTTHFHLKRKIGYFVIQTYLPCIMTVILSQVSFWLNRESVPARTVFGVTTVLTMTTLSISARNSLPKVAYATAMDWFIAVCYAFVFSALIEFATVNYFTKRGYAWDGKSVVPEKPKKVKDPLIKKNNTYAPTATSYTPNLARGDPGLATIAKSATIEPKEVKPETKPPEPKKTFNSVSKIDRLSRIAFPLLFGIFNLVYWATYLNREPQLKAPTPHQ A270B43423B4086E gbrb3_human Gamma-aminobutyric acid receptor subunit beta-3 GABRB3 GABA(A) receptor subunit beta-3 protein protein human Homo sapiens Human MWGLAGGRLFGIFSAPVLVAVVCCAQSVNDPGNMSFVKETVDKLLKGYDIRLRPDFGGPPVCVGMNIDIASIDMVSEVNMDYTLTMYFQQYWRDKRLAYSGIPLNLTLDNRVADQLWVPDTYFLNDKKSFVHGVTVKNRMIRLHPDGTVLYGLRITTTAACMMDLRRYPLDEQNCTLEIESYGYTTDDIEFYWRGGDKAVTGVERIELPQFSIVEHRLVSRNVVFATGAYPRLSLSFRLKRNIGYFILQTYMPSILITILSWVSFWINYDASAARVALGITTVLTMTTINTHLRETLPKIPYVKAIDMYLMGCFVFVFLALLEYAFVNYIFFGRGPQRQKKLAEKTAKAKNDRSKSESNRVDAHGNILLTSLEVHNEMNEVSGGIGDTRNSAISFDNSGIQYRKQSMPREGHGRFLGDRSLPHKKTHLRRRSSQLKIKIPDLTDVNAIDRWSRIVFPFTFSLFNLVYWLYYVN DIP protein P28472 original sequence version: 151 DIP protein P28472-1 original sequence version: 149 D63597F7FBC69E71 gbra1_human Gamma-aminobutyric acid receptor subunit alpha-1 GABRA1 GABA(A) receptor subunit alpha-1 protein protein human Homo sapiens Human MRKSPGLSDCLWAWILLLSTLTGRSYGQPSLQDELKDNTTVFTRILDRLLDGYDNRLRPGLGERVTEVKTDIFVTSFGPVSDHDMEYTIDVFFRQSWKDERLKFKGPMTVLRLNNLMASKIWTPDTFFHNGKKSVAHNMTMPNKLLRITEDGTLLYTMRLTVRAECPMHLEDFPMDAHACPLKFGSYAYTRAEVVYEWTREPARSVVVAEDGSRLNQYDLLGQTVDSGIVQSSTGEYVVMTTHFHLKRKIGYFVIQTYLPCIMTVILSQVSFWLNRESVPARTVFGVTTVLTMTTLSISARNSLPKVAYATAMDWFIAVCYAFVFSALIEFATVNYFTKRGYAWDGKSVVPEKPKKVKDPLIKKNNTYAPTATSYTPNLARGDPGLATIAKSATIEPKEVKPETKPPEPKKTFNSVSKIDRLSRIAFPLLFGIFNLVYWATYLNREPQLKAPTPHQ F81EC9ECBE64E94D gabrb3-arfgef2 3 7 unspecified role unspecified role prey prey c-terminal sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 6 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association no figure gabrb3-arfgef2-1 4 6 unspecified role unspecified role bait bait region required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain 7 unspecified role unspecified role prey prey c-terminal required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain physical association physical association 2b and c gabrb1-gabrb3 4 7 unspecified role unspecified role prey prey c-terminal sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain 8 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association 2b gabrb1-gabrb2 4 7 unspecified role unspecified role prey prey c-terminal sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain 9 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association 2b arfgef2-gabrb3 2 6 unspecified role unspecified role prey prey purified purified n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) 7 unspecified role unspecified role bait bait purified purified region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) direct interaction direct interaction 3a gabra1-arfgef2 5 10 unspecified role unspecified role bait bait 7 unspecified role unspecified role prey prey physical association physical association 3c arfgef2-gabra1 1 12 unspecified role unspecified role neutral component neutral component 7 unspecified role unspecified role neutral component neutral component nucl transfection nucleic acid transfection nucl transfection n-terminus ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range c-terminal sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 11 unspecified role unspecified role neutral component neutral component colocalization colocalization 4