mbinfo MBInfo http://www.mechanobio.info/ The mouse Formin mDia1 is a potent actin nucleation factor regulated by autoinhibition. The mouse Formin mDia1 is a potent actin nucleation factor regulated by autoinhibition. Curr. Biol. (0960-9822) 2003 imex curation Li F., Higgs H.N. Interaction detection: SIze-exclusion chromatography. Participant detection: Eluted fractions were monitored by absorption at 220 nm and verified by coomassie staining. The interaction of GST-RhoA with mDia1-N (Fig 4E) could not be curated since the source of GST-RhoA could not be traced. henry.n.higgs@dartmouth.edu 15-Nov-2012: Contacted by pporras. Only protein-protein interactions in vitro In vitro chromatography chromatography technology column chromatography chromatography weight identificat confirmation by molecular weight weight identificat Li F., Higgs H.N. Interaction detection: SIze-exclusion chromatography. Participant detection: Eluted fractions were monitored by absorption at 220 nm and verified by coomassie staining. The interaction of GST-RhoA with mDia1-N (Fig 4E) could not be curated since the source of GST-RhoA could not be traced. henry.n.higgs@dartmouth.edu Accepted 2012-OCT-15 by ORCHARD 15-Nov-2012: Contacted by pporras. Curr. Biol. (0960-9822) 2003 Only protein-protein interactions imex curation diap1_mouse Protein diaphanous homolog 1 Diaphanous-related formin-1 p140mDIA Diaph1 Diap1 protein protein mouse Mus musculus Mouse MEPSGGGLGPGRGTRDKKKGRSPDELPATGGDGGKHKKFLERFTSMRIKKEKEKPNSAHRNSSASYGDDPTAQSLQDISDEQVLVLFEQMLVDMNLNEEKQQPLREKDIVIKREMVSQYLHTSKAGMNQKESSRSAMMYIQELRSGLRDMHLLSCLESLRVSLNNNPVSWVQTFGAEGLASLLDILKRLHDEKEETSGNYDSRNQHEIIRCLKAFMNNKFGIKTMLETEEGILLLVRAMDPAVPNMMIDAAKLLSALCILPQPEDMNERVLEAMTERAEMDEVERFQPLLDGLKSGTSIALKVGCLQLINALITPAEELDFRVHIRSELMRLGLHQVLQELREIENEDMKVQLCVFDEQGDEDFFDLKGRLDDIRMEMDDFGEVFQIILNTVKDSKAEPHFLSILQHLLLVRNDYEARPQYYKLIEECVSQIVLHKNGTDPDFKCRHLQIDIERLVDQMIDKTKVEKSEAKATELEKKLDSELTARHELQVEMKKMENDFEQKLQDLQGEKDALDSEKQQITAQKQDLEAEVSKLTGEVAKLSKELEDAKNEMASLSAVVVAPSVSSSAAVPPAPPLPGDSGTVIPPPPPPPPLPGGVVPPSPPLPPGTCIPPPPPLPGGACIPPPPQLPGSAAIPPPPPLPGVASIPPPPPLPGATAIPPPPPLPGATAIPPPPPLPGGTGIPPPPPPLPGSVGVPPPPPLPGGPGLPPPPPPFPGAPGIPPPPPGMGVPPPPPFGFGVPAAPVLPFGLTPKKVYKPEVQLRRPNWSKFVAEDLSQDCFWTKVKEDRFENNELFAKLTLAFSAQTKTSKAKKDQEGGEEKKSVQKKKVKELKVLDSKTAQNLSIFLGSFRMPYQEIKNVILEVNEAVLTESMIQNLIKQMPEPEQLKMLSELKEEYDDLAESEQFGVVMGTVPRLRPRLNAILFKLQFSEQVENIKPEIVSVTAACEELRKSENFSSLLELTLLVGNYMNAGSRNAGAFGFNISFLCKLRDTKSADQKMTLLHFLAELCENDHPEVLKFPDELAHVEKASRVSAENLQKSLDQMKKQIADVERDVQNFPAATDEKDKFVEKMTSFVKDAQEQYNKLRMMHSNMETLYKELGDYFVFDPKKLSVEEFFMDLHNFRNMFLQAVKENQKRRETEEKMRRAKLAKEKAEKERLEKQQKREQLIDMNAEGDETGVMDSLLEALQSGAAFRRKRGPRQVNRKAGCAVTSLLASELTKDDAMAPGPVKVPKKSEGVPTILEEAKELVGRAS DIP protein O08808 original sequence version: 62 DIP protein O08808 original sequence version: 141 09404164873CA7C1 diaph1-diaph1-1 1 2 unspecified role unspecified role neutral component neutral component n-terminal region sufficient to bind sufficient binding region sufficient to bind n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) 2 unspecified role unspecified role neutral component neutral component c-terminal region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain This region includes the FH2 domain of the mouse mDia1 protein. ecoli Escherichia coli (strain K12) 5 7 physical association physical association Fig 4C.